Amplicon-based profiling of bacteria in raw and secondary treated wastewater from treatment plants across Australia.

abstract：:The operational temperature of microbial fuel cell reactors influences biofilm development, and this has an impact on anodic biocatalytic activity. In this study, we compared three microbial fuel cell (MFC) reactors acclimated at 10°C, 20°C and 35°C to investigate the effect on biomass development, methanogenesis and ...

journal_title：Applied microbiology and biotechnology

authors： Michie IS,Kim JR,Dinsdale RM,Guwy AJ,Premier GC

更新日期：2011-10-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:The primary explosive found in most land mines, 2,4,6-trinitrotoluene (2,4,6-TNT), is often accompanied by 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB) impurities. The latter two compounds, being more volatile, have been reported to slowly leak through land mine covers and permeate the soil under whic...

journal_title：Applied microbiology and biotechnology

authors： Yagur-Kroll S,Lalush C,Rosen R,Bachar N,Moskovitz Y,Belkin S

更新日期：2014-01-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the...

journal_title：Applied microbiology and biotechnology

authors： Parhiz H,Ketcham SA,Zou G,Ghosh B,Fratz-Berilla EJ,Ashraf M,Ju T,Madhavarao CN

更新日期：2019-08-01 00:00:00

abstract：:Organophosphate hydrolase containing hexahistidine tag at the N-terminus of recombinant protein (His6-OPH) and expressed in Escherichia coli cells was purified using supermacroporous polyacrylamide-based monolith columns with immobilized metal affinity matrices [Me2+-iminodiacetic acid (IDA)-polyacrylamide cryogel (PA...

journal_title：Applied microbiology and biotechnology

authors： Efremenko E,Votchitseva Y,Plieva F,Galaev I,Mattiasson B

更新日期：2006-05-01 00:00:00

abstract：:Biochemical processes in biogas plants are still not fully understood. Especially, the identification of possible bottlenecks in the complex fermentation processes during biogas production might provide potential to increase the performance of biogas plants. To shed light on the question which group of organism consti...

journal_title：Applied microbiology and biotechnology

authors： Refai S,Wassmann K,Deppenmeier U

更新日期：2014-08-01 00:00:00

abstract：:Sulfation is an important way for detoxifying xenobiotics and endobiotics including catechols. Enzymatic sulfation occurs usually with high chemo- and/or regioselectivity under mild reaction conditions. In this study, a two-step p-NPS-4-AAP screening system for laboratory evolution of aryl sulfotransferase B (ASTB) wa...

journal_title：Applied microbiology and biotechnology

authors： Ji Y,Islam S,Mertens AM,Sauer DF,Dhoke GV,Jakob F,Schwaneberg U

更新日期：2019-05-01 00:00:00

abstract：:Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from ...

journal_title：Applied microbiology and biotechnology

authors： Leite HA,Silva AB,Gomes FP,Gramacho KP,Faria JC,de Souza JT,Loguercio LL

更新日期：2013-03-01 00:00:00

abstract：:Exposure to bioaerosols can adversely influence human health through respiratory tract, eye, and skin irritation. Bioaerosol composition is unique on the International Space Station (ISS), where the size distribution of particles in the air differs from those on Earth. This is due to the lack of gravitational settling...

journal_title：Applied microbiology and biotechnology

authors： Haines SR,Bope A,Horack JM,Meyer ME,Dannemiller KC

更新日期：2019-09-01 00:00:00

abstract：:Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S...

journal_title：Applied microbiology and biotechnology

authors： Bräu B,Hilgenfeld R,Schlingmann M,Marquardt R,Birr E,Wohlleben W,Aufderheide K,Pühler A

更新日期：1991-01-01 00:00:00

abstract：:Escherichia coli-the powerhouse for recombinant protein production-is rapidly gaining status as a reliable and efficient host for secretory expression. An improved understanding of protein translocation processes and its mechanisms has inspired and accelerated the development of new tools and applications in this fiel...

journal_title：Applied microbiology and biotechnology

authors： Low KO,Muhammad Mahadi N,Md Illias R

更新日期：2013-05-01 00:00:00

abstract：:The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

journal_title：Applied microbiology and biotechnology

authors： Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

更新日期：2014-04-01 00:00:00

abstract：:Soil metagenome constitutes a reservoir for discovering novel enzymes from the unculturable microbial diversity. From three plant rhizosphere metagenomic libraries comprising a total of 142,900 members of recombinant plasmids, we obtained 14 recombinant fosmids that exhibited lipolytic activity. A selected recombinant...

journal_title：Applied microbiology and biotechnology

authors： Lee MH,Hong KS,Malhotra S,Park JH,Hwang EC,Choi HK,Kim YS,Tao W,Lee SW

更新日期：2010-11-01 00:00:00

abstract：:Economical production of medium-chain length polyhydroxyalkanoates (mcl-PHA) is dependent on efficient cultivation processes. This work describes growth and mcl-PHA synthesis characteristics of Pseudomonas putida LS46 when grown on medium-chain length fatty acids (octanoic acid) and lower-cost long-chain fatty acids (...

journal_title：Applied microbiology and biotechnology

authors： Blunt W,Dartiailh C,Sparling R,Gapes D,Levin DB,Cicek N

更新日期：2018-08-01 00:00:00

abstract：:The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 d...

journal_title：Applied microbiology and biotechnology

authors： Corchero JL,Carbonell X,Benito A,Villaverde A

更新日期：1995-03-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:Pneumocandin B0, the precursor of the antifungal drug caspofungin, is a secondary metabolite of the fungus Glarea lozoyensis. In this study, we investigated the effects of mannitol as the sole carbon source on pneumocandin B0 production by G. lozoyensis. The osmotic pressure is more important in enhancing pneumocandin...

journal_title：Applied microbiology and biotechnology

authors： Song P,Huang B,Zhang S,Zhang K,Yuan K,Ji X,Ren L,Wen J,Huang H

更新日期：2018-12-01 00:00:00

abstract：:Atrazine is transformed to N-isopropylammelide through hydroxyatrazine as an intermediate as indicated by high-performance liquid chromatography/mass spectroscopy in culture filtrates of Frankia alni ACN14a and Frankia sp. EuI1c. Both Frankia strains have the ability to degrade atrazine via dechlorination and dealkyla...

journal_title：Applied microbiology and biotechnology

authors： Rehan M,Kluge M,Fränzle S,Kellner H,Ullrich R,Hofrichter M

更新日期：2014-07-01 00:00:00

abstract：:Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

journal_title：Applied microbiology and biotechnology

authors： Peirotén Á,Landete JM

更新日期：2020-05-01 00:00:00

abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Zhao X,Bai F

更新日期：2013-05-01 00:00:00

abstract：:Aliphatic ketones, such as 2-butanone and 3-hexanone, with only one carbon difference among side chains adjacent to the carbonyl carbon are difficult to be reduced enantioselectively. In this study, we utilized an acetophenone reductase from Geotrichum candidum NBRC 4597 (GcAPRD) to reduce challenging aliphatic ketone...

journal_title：Applied microbiology and biotechnology

authors： Koesoema AA,Sugiyama Y,Xu Z,Standley DM,Senda M,Senda T,Matsuda T

更新日期：2019-12-01 00:00:00

abstract：:Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

journal_title：Applied microbiology and biotechnology

authors： Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

更新日期：2001-07-01 00:00:00

abstract：:A thermophilic Bacillus coagulans WCP10-4 with tolerance to high concentration of glucose was isolated from soil and used to produce optically pure L-lactic acid from glucose and starch. In batch fermentation at pH 6.0, 240 g/L of glucose was completely consumed giving 210 g/L of L-lactic acid with a yield of 95 % and...

journal_title：Applied microbiology and biotechnology

authors： Zhou X,Ye L,Wu JC

更新日期：2013-05-01 00:00:00

abstract：:As an alternative class of antimicrobial agents used to overcome drug-resistant infections, antimicrobial peptides (AMPs) have recently gained significant attention. In this study, we designed an improved antimicrobial peptide, K1K8, based on the molecular template of Hp1404. Compared to the wild-type Hp1404, K1K8 sho...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Liu G,Meng L,Yu W,Xu X,Li W,Wu Y,Cao Z

更新日期：2016-06-01 00:00:00

abstract：:The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic ni...

journal_title：Applied microbiology and biotechnology

authors： Saha BC

更新日期：2006-10-01 00:00:00

abstract：:The insertion of peptides is a biotechnology tool widely used to improve the nutraceutical properties of proteins. Because the effect of these insertions in protein stability and function is difficult to predict, it should be determined experimentally. In this study, we created two variants of amarantin acidic subunit...

journal_title：Applied microbiology and biotechnology

authors： Morales-Camacho JI,Espinosa-Hernández E,Fernández-Velasco DA,Benítez-Cardoza CG,Luna-Suárez S

更新日期：2018-11-01 00:00:00

abstract：:Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-E...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Guo C,Zhou L,Zhang Z,Huang Y,Yang J,Bai X,Yang K

更新日期：2015-09-01 00:00:00

abstract：:Penicillin G acylase from Achromobacter sp. (NPGA) was studied in the enzymatic synthesis of β-lactam antibiotics by kinetically controlled N-acylation. When compared with penicillin acylase of Escherichia coli (PGA), the NPGA was significantly more efficient at syntheses of ampicillin and amoxicillin (higher S/H rati...

journal_title：Applied microbiology and biotechnology

authors： Bečka S,Štěpánek V,Vyasarayani RW,Grulich M,Maršálek J,Plháčková K,Dobišová M,Marešová H,Plačková M,Valešová R,Palyzová A,Datla A,Ashar TK,Kyslík P

更新日期：2014-02-01 00:00:00

abstract：:Cell proliferation is achieved through numerous enzyme reactions. Temperature governs the activity of each enzyme, ultimately determining the optimal growth temperature. The synthesis of useful chemicals and fuels utilizes a fraction of available metabolic pathways, primarily central metabolic pathways including glyco...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Toyoda K,Matsuzawa H,Mizuno H,Ninomiya K,Takahashi K,Inui M,Tsuge Y

更新日期：2020-08-01 00:00:00