Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller".

abstract：:Thirty-four strains of lactic acid bacteria (seven Bifidobacterium, 11 Lactobacillus, six Lactococcus, and 10 Streptococcus thermophilus) were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation (TAA(AA) and TAA(LA)), trolox-equivalent antioxidant capacity (TEAC), intracellular glut...

journal_title：Applied microbiology and biotechnology

authors： Amaretti A,di Nunzio M,Pompei A,Raimondi S,Rossi M,Bordoni A

更新日期：2013-01-01 00:00:00

abstract：:The genes encoding the mevalonate-based farnesyl pyrophosphate (FPP) biosynthetic pathway were encoded in two operons and expressed in Escherichia coli to increase the production of sesquiterpenes. Inefficient translation of several pathway genes created bottlenecks and led to the accumulation of several pathway inter...

journal_title：Applied microbiology and biotechnology

authors： Nowroozi FF,Baidoo EE,Ermakov S,Redding-Johanson AM,Batth TS,Petzold CJ,Keasling JD

更新日期：2014-02-01 00:00:00

abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Zhao X,Bai F

更新日期：2013-05-01 00:00:00

abstract：:The substrate selectivity of numerous commercially available lipases from microorganisms, plants and animal tissue towards 9-octadecenoic acids with respect to the cis/trans configuration of the C=C double bond was examined by the esterification of cis- and trans-9-octadecanoic acid (oleic and elaidic acid respectivel...

journal_title：Applied microbiology and biotechnology

authors： Borgdorf R,Warwel S

更新日期：1999-04-01 00:00:00

abstract：:Polyunsaturated fatty acids, docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3), and arachidonic acid (ARA, 20:4 n-6), have multiple beneficial effects on human health and can be used as an important ingredient in dietary supplements, food, feed and pharmaceuticals. A variety of microorganis...

journal_title：Applied microbiology and biotechnology

authors： Wu L,Roe CL,Wen Z

更新日期：2013-09-01 00:00:00

abstract：:Lipoxygenase (LOX; EC 1.13.11.12) is an enzyme which is widely used in food industry to improve aroma and rheological or baking properties of foods. A series of studies have proven that the flexible regions negatively relates to the thermal stability of enzymes. In this study, two highly flexible regions, residues(20-...

journal_title：Applied microbiology and biotechnology

authors： Lu X,Liu S,Feng Y,Rao S,Zhou X,Wang M,Du G,Chen J

更新日期：2014-02-01 00:00:00

abstract：:The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular mass of 50,805 Da. The open reading fra...

journal_title：Applied microbiology and biotechnology

authors： Arai A,Masuda S,Matsuyama A,Murakami S,Nakajima M

更新日期：1998-03-01 00:00:00

abstract：:This study monitored changes in bacterial and fungal structure in a mine water in a monthly basis over 4 months. Over the 4-month study period, mine water samples contained more bacteria (91.06 %) compared to fungi (8.94 %). For bacteria, mine water samples were dominated by Proteobacteria (39.14 to 65.06 %) followed ...

journal_title：Applied microbiology and biotechnology

authors： Kamika I,Azizi S,Tekere M

更新日期：2016-07-01 00:00:00

abstract：:For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement...

journal_title：Applied microbiology and biotechnology

authors： Sindelar G,Wendisch VF

更新日期：2007-09-01 00:00:00

abstract：:Microbial aggregates of an aerobic granular sludge can be used for the treatment of industrial or municipal wastewater, but their formation from a microbial activated sludge requires several weeks. Therefore, the aim of this research was the selection of microbial cultures to shorten the granule-forming period from se...

journal_title：Applied microbiology and biotechnology

authors： Ivanov V,Wang XH,Tay ST,Tay JH

更新日期：2006-04-01 00:00:00

abstract：:High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...

journal_title：Applied microbiology and biotechnology

authors： Hua D,Ma C,Song L,Lin S,Zhang Z,Deng Z,Xu P

更新日期：2007-03-01 00:00:00

abstract：:Nitrile-converting enzymes are becoming commonplace in the synthesis of pharmaceuticals and commodity chemicals. These versatile biocatalysts have potential applications in different fields including synthetic biocatalysis and bioremediation. This review attempts to describe in detail the three major classes of nitril...

journal_title：Applied microbiology and biotechnology

authors： Banerjee A,Sharma R,Banerjee UC

更新日期：2002-10-01 00:00:00

abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

journal_title：Applied microbiology and biotechnology

authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

更新日期：2002-10-01 00:00:00

abstract：:For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The su...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Xie Y,Yu Z,Meng G,Wu Z

更新日期：2019-10-01 00:00:00

abstract：:Batch assays are currently used to study the kinetic behavior of microbial growth. However, it has been shown that the outcome of batch experiments is greatly influenced by the initial ratio of substrate concentration (S(o)) to biomass concentration (Xo). Substrate-sufficient batch culture is known to have mechanisms ...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Chen GH,Rols JL

更新日期：1999-11-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00

abstract：:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatograph...

journal_title：Applied microbiology and biotechnology

authors： Wu G,Wang X,Deng X,Wu P,Xue X,Yan X,Wang X

更新日期：2012-03-01 00:00:00

abstract：:By damaging DNA molecules, genotoxicants cause genetic mutations and also increase human susceptibility to cancers and genetic diseases. Over the past four decades, several assays have been developed in the budding yeast Saccharomyces cerevisiae to screen potential genotoxic substances and provide alternatives to anim...

journal_title：Applied microbiology and biotechnology

authors： Eki T

更新日期：2018-03-01 00:00:00

abstract：:A Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally hig...

journal_title：Applied microbiology and biotechnology

authors： Stankovic N,Radulovic V,Petkovic M,Vuckovic I,Jadranin M,Vasiljevic B,Nikodinovic-Runic J

更新日期：2012-12-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00

abstract：:Quorum sensing gives rise to biofilm formation on the membrane surface, which in turn causes a loss of water permeability in membrane bioreactors (MBRs) for wastewater treatment. Enzymatic quorum quenching was reported to successfully inhibit the formation of biofilm in MBRs through the decomposition of signal molecul...

journal_title：Applied microbiology and biotechnology

authors： Kim HW,Oh HS,Kim SR,Lee KB,Yeon KM,Lee CH,Kim S,Lee JK

更新日期：2013-05-01 00:00:00

abstract：:Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1)...

journal_title：Applied microbiology and biotechnology

authors： Chandrakant P,Bisaria VS

更新日期：2000-03-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:The goal of sustainable production of biochemicals and biofuels has driven the engineering of microbial cell as factories that convert low-value substrates to high-value products. Xylose is the second most abundant sugar substrate in lignocellulosic hydrolysates. We analyzed the mechanisms of xylose metabolism using g...

journal_title：Applied microbiology and biotechnology

authors： Kim D,Woo HM

更新日期：2018-11-01 00:00:00

abstract：:Kimchi, a traditional Korean food made by the fermentation of vegetables, has become popular globally because of its organoleptic, beneficial, and nutritional properties. Spontaneous kimchi fermentation in unsterilized raw materials leads to the growth of various lactic acid bacteria (LAB), which results in variations...

journal_title：Applied microbiology and biotechnology

authors： Jung JY,Lee SH,Jeon CO

更新日期：2014-03-01 00:00:00

abstract：:Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from ...

journal_title：Applied microbiology and biotechnology

authors： Leite HA,Silva AB,Gomes FP,Gramacho KP,Faria JC,de Souza JT,Loguercio LL

更新日期：2013-03-01 00:00:00

abstract：:Protein turnover through de novo synthesis is critical for sustainable cellular functions. We previously found that glucose consumption rate in Corynebacterium glutamicum under anaerobic conditions increased at temperature higher than the upper limit of growth temperature. Here, we showed that production of lactic and...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Ninomiya K,Takahashi K,Tsuge Y

更新日期：2020-05-01 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00