当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Enhanced thermal stability of Pseudomonas aeruginosa lipoxygenase through modification of two highly flexible regions.

Enhanced thermal stability of Pseudomonas aeruginosa lipoxygenase through modification of two highly flexible regions.

Abstract：

:Lipoxygenase (LOX; EC 1.13.11.12) is an enzyme which is widely used in food industry to improve aroma and rheological or baking properties of foods. A series of studies have proven that the flexible regions negatively relates to the thermal stability of enzymes. In this study, two highly flexible regions, residues(20-49) and residues(201-206), were modified to improve the thermal stability of LOX from Pseudomonas aeruginosa. Deletion of the first 20 and 30 residues of the former region increased the thermal stability of the LOX by 1.3- and 2.1-fold, respectively. Although deletion of the residues(201-206) led to a sharp reduction of both thermal stability and catalytic activity of the enzyme, the residue substitutions with the glycines (G204P, G206P, and G204P/G206P) or even glycine-rich linker (L6/PT) within this region increased the thermal stability of LOX by values ranging from 0.46- to 3.45-fold. To be noted, over 85% of the specific activity was maintained in all thermally stabilized LOX mutants. Circular dichroism and fluorescence analysis showed that the overall secondary and tertiary structures were not significantly changed by these modifications. To the best of our knowledge, this is the first report on increasing the thermal stability of LOX by protein engineering without remarkably affecting the catalytic rate.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Lu X,Liu S,Feng Y,Rao S,Zhou X,Wang M,Du G,Chen J

doi

10.1007/s00253-013-5039-y

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

1663-9

issue

4

eissn

0175-7598

issn

1432-0614

journal_volume

98

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • New aspects and strategies for methane mitigation from ruminants.

    abstract：:The growing demand for sustainable animal production is compelling researchers to explore the potential approaches to reduce emissions of greenhouse gases from livestock that are mainly produced by enteric fermentation. Some potential solutions, for instance, the use of chemical inhibitors to reduce methanogenesis, ar...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-013-5365-0

    authors： Kumar S,Choudhury PK,Carro MD,Griffith GW,Dagar SS,Puniya M,Calabro S,Ravella SR,Dhewa T,Upadhyay RC,Sirohi SK,Kundu SS,Wanapat M,Puniya AK

    更新日期：2014-01-01 00:00:00

  • Synergistic antidigestion effect of Lactobacillus rhamnosus and bovine colostrums in simulated gastrointestinal tract (in vitro).

    abstract：:Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-0861-8

    authors： Wei H,Xu Y,Xiong Y,Xu F,Liu G

    更新日期：2007-06-01 00:00:00

  • Tandem repeat-tRNA (TRtRNA) PCR method for the molecular typing of non-Saccharomyces subspecies.

    abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3714-4

    authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

    更新日期：2012-01-01 00:00:00

  • Identification of a chitosanase from the marine metagenome and its molecular improvement based on evolution data.

    abstract：:Chitooligosaccharides have important application value in the fields of food and agriculture. Chitosanase can degrade chitosan to obtain chitooligosaccharides. The marine metagenome contains many genes related to the degradation of chitosan. However, it is difficult to mine valuable genes from large gene resources. Th...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10715-8

    authors： Han Y,Guan F,Sun J,Wu N,Tian J

    更新日期：2020-08-01 00:00:00

  • Differential modulation of avian β-defensin and Toll-like receptor expression in chickens infected with infectious bronchitis virus.

    abstract：:The host innate immune response either clears invading viruses or allows the adaptive immune system to establish an effective antiviral response. In this study, both pathogenic (passage 3, P3) and attenuated (P110) infectious bronchitis virus (IBV) strains were used to study the immune responses of chicken to IBV infe...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6786-8

    authors： Xu Y,Zhang T,Xu Q,Han Z,Liang S,Shao Y,Ma D,Liu S

    更新日期：2015-11-01 00:00:00

  • Carbon flux to growth or polyhydroxyalkanoate synthesis under microaerophilic conditions is affected by fatty acid chain-length in Pseudomonas putida LS46.

    abstract：:Economical production of medium-chain length polyhydroxyalkanoates (mcl-PHA) is dependent on efficient cultivation processes. This work describes growth and mcl-PHA synthesis characteristics of Pseudomonas putida LS46 when grown on medium-chain length fatty acids (octanoic acid) and lower-cost long-chain fatty acids (...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9055-9

    authors： Blunt W,Dartiailh C,Sparling R,Gapes D,Levin DB,Cicek N

    更新日期：2018-08-01 00:00:00

  • Biosynthesis of nervonic acid and perspectives for its production by microalgae and other microorganisms.

    abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-8859-y

    authors： Fan Y,Meng HM,Hu GR,Li FL

    更新日期：2018-04-01 00:00:00

  • Expression, purification, and characterization of the native intracellular domain of human epidermal growth factor receptors 1 and 2 in Escherichia coli.

    abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-10116-6

    authors： Seetaha S,Ratanabanyong S,Choowongkomon K

    更新日期：2019-10-01 00:00:00

  • L-valine, an antialgal amino acid from Streptomyces jiujiangensis JXJ 0074(T).

    abstract：:An antialgal compound was isolated from the cultured broth of Streptomyces jiujiangensis JXJ 0074(T) by using bioassay methods. Based on the data of (1)H-NMR, (13)C-NMR, ESI-MS, and thin layer chromatography, the active compound was identified as L-valine, which showed antialgal activity mainly against Microcystis. L-...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7150-8

    authors： Zhang BH,Chen W,Li HQ,Yang JY,Zha DM,Duan YQ,N Hozzein W,Xiao M,Gao R,Li WJ

    更新日期：2016-05-01 00:00:00

  • Improved sandwich-hybridization assay for an electrical DNA-chip-based monitoring of bioprocess-relevant marker genes.

    abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1347-z

    authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

    更新日期：2008-03-01 00:00:00

  • Cytochromes P450 as promising catalysts for biotechnological application: chances and limitations.

    abstract：:Cytochromes P450 (CYPs) belong to the superfamily of heme b containing monooxygenases with currently more than 21,000 members. These enzymes accept a vast range of organic molecules and catalyze diverse reactions. These extraordinary capabilities of CYP systems that are unmet by other enzymes make them attractive for ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-014-5767-7

    authors： Bernhardt R,Urlacher VB

    更新日期：2014-01-01 00:00:00

  • Cell-targeting aptamers act as intracellular delivery vehicles.

    abstract：:Aptamers are single-stranded nucleic acids or peptides identified from a randomized combinatorial library through specific interaction with the target of interest. Targets can be of any size, from small molecules to whole cells, attesting to the versatility of aptamers for binding a wide range of targets. Aptamers sho...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-016-7686-2

    authors： Gopinath SC,Lakshmipriya T,Chen Y,Arshad MK,Kerishnan JP,Ruslinda AR,Al-Douri Y,Voon CH,Hashim U

    更新日期：2016-08-01 00:00:00

  • Introduction of green fluorescent protein gene into phenol-degrading Alcaligenes faecalis cells and their monitoring in phenol-contaminated soil.

    abstract：:Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530100614

    authors： Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

    更新日期：2001-07-01 00:00:00

  • A single-plasmid vector for transgene amplification using short hairpin RNA targeting the 3'-UTR of amplifiable dhfr.

    abstract：:Gene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorp...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6856-y

    authors： Kang SY,Kim YG,Lee HW,Lee EG

    更新日期：2015-12-01 00:00:00

  • Effect of the osmotic conditions during sporulation on the subsequent resistance of bacterial spores.

    abstract：:The causes of Bacillus spore resistance remain unclear. Many structures including a highly compact envelope, low hydration of the protoplast, high concentrations of Ca-chelated dipicolinic acid, and the presence of small acid-soluble spore proteins seem to contribute to resistance. To evaluate the role of internal pro...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1519-x

    authors： Nguyen Thi Minh H,Guyot S,Perrier-Cornet JM,Gervais P

    更新日期：2008-08-01 00:00:00

  • Generation of biologically contained, readily transformable, and genetically manageable mutants of the biotechnologically important Bacillus pumilus.

    abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-4935-5

    authors： Wemhoff S,Meinhardt F

    更新日期：2013-09-01 00:00:00

  • From photons to biomass and biofuels: evaluation of different strategies for the improvement of algal biotechnology based on comparative energy balances.

    abstract：:Microalgal based biofuels are discussed as future sustainable energy source because of their higher photosynthetic and water use efficiency to produce biomass. In the context of climate CO2 mitigation strategies, algal mass production is discussed as a potential CO2 sequestration technology which uses CO2 emissions to...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-011-3627-2

    authors： Wilhelm C,Jakob T

    更新日期：2011-12-01 00:00:00

  • Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase.

    abstract：:Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4542-x

    authors： Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

    更新日期：2013-08-01 00:00:00

  • Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5.

    abstract：:The upstream region of the cellobiohydrolase gene cbhA of Clostridium thermocellum F7 was sequenced. It was found that this region contains the previously sequenced gene celK encoding an enzyme closely related to CbhA (cellulosomal subunit S3). The presence of a putative transcription terminator in the 524-bp intergen...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051473

    authors： Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

    更新日期：1999-06-01 00:00:00

  • Bacterial growth in space flight: logistic growth curve parameters for Escherichia coli and Bacillus subtilis.

    abstract：:Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051386

    authors： Kacena MA,Merrell GA,Manfredi B,Smith EE,Klaus DM,Todd P

    更新日期：1999-02-01 00:00:00

  • Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries.

    abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0347-0

    authors： Krüger C,Hultberg A,Marcotte H,Hermans P,Bezemer S,Frenken LG,Hammarström L

    更新日期：2006-10-01 00:00:00

  • Development of chitosan-coated agar-gelatin particles for probiotic delivery and targeted release in the gastrointestinal tract.

    abstract：:This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10632-w

    authors： Albadran HA,Monteagudo-Mera A,Khutoryanskiy VV,Charalampopoulos D

    更新日期：2020-07-01 00:00:00

  • Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Müller".

    abstract：:Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00180575

    authors： Bräu B,Hilgenfeld R,Schlingmann M,Marquardt R,Birr E,Wohlleben W,Aufderheide K,Pühler A

    更新日期：1991-01-01 00:00:00

  • Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation.

    abstract：:Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1409-2

    authors： Barrios-González J,Baños JG,Covarrubias AA,Garay-Arroyo A

    更新日期：2008-05-01 00:00:00

  • Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field.

    abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9347-0

    authors： Shen G,Zhang S,Liu X,Jiang Q,Ding W

    更新日期：2018-11-01 00:00:00

  • Chemical diversity of biologically active metabolites in the sclerotia of Inonotus obliquus and submerged culture strategies for up-regulating their production.

    abstract：:Inonotus obliquus (Fr.) Pilat is a white rot fungus belonging to the family Hymenochaetaceae in the Basidiomycota. In nature, this fungus rarely forms a fruiting body but usually an irregular shape of sclerotial conk called 'Chaga'. Characteristically, I. obliquus produces massive melanins released to the surface of C...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-010-2682-4

    authors： Zheng W,Miao K,Liu Y,Zhao Y,Zhang M,Pan S,Dai Y

    更新日期：2010-07-01 00:00:00

  • Bioleaching review part B: progress in bioleaching: applications of microbial processes by the minerals industries.

    abstract：:This review describes the historical development and current state of metals leaching and sulfide mineral biooxidation by the minerals industries. During the past 20 years commercial processes employing microorganisms for mineral recovery have progressed from rather uncontrolled copper dump leaching to mineral oxidati...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-003-1404-6

    authors： Olson GJ,Brierley JA,Brierley CL

    更新日期：2003-12-01 00:00:00

  • Changes in intestinal bacterial communities are closely associated with shrimp disease severity.

    abstract：:Increasing evidence has revealed a close association between intestinal bacterial communities and human health. However, given that host phylogeny shapes the composition of intestinal microbiota, it is unclear whether changes in intestinal microbiota structure in relation to shrimp health status. In this study, we col...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6632-z

    authors： Xiong J,Wang K,Wu J,Qiuqian L,Yang K,Qian Y,Zhang D

    更新日期：2015-08-01 00:00:00

  • Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana.

    abstract：:Genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as Beauveria bassiana. To date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4426-0

    authors： Ying SH,Feng MG,Keyhani NO

    更新日期：2013-04-01 00:00:00

  • Direct electrochemical immunoassay based on a silica nanoparticles/sol-gel composite architecture for encapsulation of immunoconjugate.

    abstract：:A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol-gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this membrane could be used for reagen...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0353-2

    authors： Wang FC,Yuan R,Chai YQ

    更新日期：2006-10-01 00:00:00