Direct electrochemical immunoassay based on a silica nanoparticles/sol-gel composite architecture for encapsulation of immunoconjugate.

abstract：:The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

journal_title：Applied microbiology and biotechnology

authors： Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

更新日期：2014-04-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permea...

journal_title：Applied microbiology and biotechnology

authors： Pérez-García F,Peters-Wendisch P,Wendisch VF

更新日期：2016-09-01 00:00:00

abstract：:We describe the discovery, isolation and characterization of a highly thermostable alditol oxidase from Acidothermus cellulolyticus 11B. This protein was identified by searching the genomes of known thermophiles for enzymes homologous to Streptomyces coelicolor A3(2) alditol oxidase (AldO). A gene (sharing 48% protein...

journal_title：Applied microbiology and biotechnology

authors： Winter RT,Heuts DP,Rijpkema EM,van Bloois E,Wijma HJ,Fraaije MW

更新日期：2012-07-01 00:00:00

abstract：:A large percentage of organic fuel consumed in a microbial fuel cell (MFC) is lost as a result of oxygen transfer through the cathode. In order to understand how this oxygen transfer affects the microbial community structure, reactors were operated in duplicate using three configurations: closed circuit (CC; with curr...

journal_title：Applied microbiology and biotechnology

authors： Shehab N,Li D,Amy GL,Logan BE,Saikaly PE

更新日期：2013-11-01 00:00:00

abstract：:The objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. We identified and selected specific genes to detect Yersinia pestis, Francisella tularensis, Burkholderia mallei, Burkhol...

journal_title：Applied microbiology and biotechnology

authors： Carrera M,Sagripanti JL

更新日期：2009-01-01 00:00:00

abstract：:Two enzymatic systems were developed for the efficient synthesis of glycoside products of α-mangostin, a natural xanthonoid exhibiting anti-oxidant, antibacterial, anti-inflammatory, and anticancer activities. In these systems, one-pot reactions for the synthesis of UDP-α-D-glucose and UDP-α-D-2-deoxyglucose were modi...

journal_title：Applied microbiology and biotechnology

authors： Le TT,Pandey RP,Gurung RB,Dhakal D,Sohng JK

更新日期：2014-10-01 00:00:00

abstract：:Yarrowia lipolytica is an industrial yeast that has been used in the sustainable production of fatty acid-derived and lipid compounds due to its high growth capacity, genetic tractability, and oleaginous properties. This investigation examines the possibility of utilizing urea or urine as an alternative to ammonium su...

journal_title：Applied microbiology and biotechnology

authors： Brabender M,Hussain MS,Rodriguez G,Blenner MA

更新日期：2018-03-01 00:00:00

abstract：:Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were...

journal_title：Applied microbiology and biotechnology

authors： Zheng Y,Yang W,Sun X,Wang SP,Rui YC,Luo CY,Guo LD

更新日期：2012-03-01 00:00:00

abstract：:The fungus Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean (Phaseolus vulgaris), and anthracnose is one of the most devastating diseases of this plant species. However, little is known about the proteins that are essential for the fungus-plant interactions. Knowledge of the fungus' ...

journal_title：Applied microbiology and biotechnology

authors： de Queiroz CB,Correia HLN,Santana MF,Batista DS,Vidigal PMP,Brommonschenkel SH,de Queiroz MV

更新日期：2019-03-01 00:00:00

abstract：:Due to their high secretion capacity, Gram-positive bacteria from the genus Bacillus are important expression hosts for the high-yield production of enzymes in industrial biotechnology; however, to date, strains from only few Bacillus species are used for enzyme production at industrial scale. Herein, we introduce Pae...

journal_title：Applied microbiology and biotechnology

authors： Heinze S,Zimmermann K,Ludwig C,Heinzlmeir S,Schwarz WH,Zverlov VV,Liebl W,Kornberger P

更新日期：2018-12-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:The present study emphasised on the development of new mathematical models based on mass balance and stoichiometry of nitrogen removal in anammox hybrid reactor (AHR). The performance of AHR at varying hydraulic retention times (HRTs) and nitrogen loading rates (NLRs) revealed that nitrogen removal efficiency (NRE) in...

journal_title：Applied microbiology and biotechnology

authors： Tomar S,Gupta SK

更新日期：2015-11-01 00:00:00

abstract：:Bioconversion of cassava-derived glucose to 2-keto-D-gluconic acid (2-KDG) using resting cells of immobilized Pseudomonas aeruginosa IFO 3448 was investigated. The tuberous roots of cassava were selected as the feedstock as they are inexpensive and widely available, and possess high amounts of starch (approximately 70...

journal_title：Applied microbiology and biotechnology

authors： Chia M,Van Nguyen TB,Choi WJ

更新日期：2008-04-01 00:00:00

abstract：:In this study, a high yield production bioprocess with recombinant Bacillus megaterium for the production of the extracellular enzyme levansucrase (SacB) was developed. For basic optimization of culture parameters and nutrients, a recombinant B. megaterium reporter strain that produced green fluorescent protein under ...

journal_title：Applied microbiology and biotechnology

authors： Korneli C,Biedendieck R,David F,Jahn D,Wittmann C

更新日期：2013-04-01 00:00:00

abstract：:Internal fragments of alpha- and beta-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5'- and 3'-rapid amplification of cDNA ends. Phytophthora capsici alpha- and beta-tubulin specific primers were then used to generate full-length cDNA b...

journal_title：Applied microbiology and biotechnology

authors： Koo BS,Park H,Kalme S,Park HY,Han JW,Yeo YS,Yoon SH,Kim SJ,Lee CM,Yoon MY

更新日期：2009-03-01 00:00:00

abstract：:The effects of three heavy metals (Co, Ni and Cd) on the growth kinetics of five bacterial strains with different characteristics (Pseudomonas sp., Phaeobacter sp. strain 27-4, Listonella anguillarum, Carnobacterium piscicola and Leuconostoc mesenteroides subsp. lysis) were studied in a batch system. A bivariate model...

journal_title：Applied microbiology and biotechnology

authors： Rial D,Vázquez JA,Murado MA

更新日期：2011-05-01 00:00:00

abstract：:Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylation profile with that of higher eukaryotes. The yeast strain Pichia pastoris is an industrially important organ...

journal_title：Applied microbiology and biotechnology

authors： Hopkins D,Gomathinayagam S,Lynaugh H,Stadheim TA,Hamilton SR

更新日期：2014-03-01 00:00:00

abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...

journal_title：Applied microbiology and biotechnology

authors： Fan Y,Meng HM,Hu GR,Li FL

更新日期：2018-04-01 00:00:00

abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

journal_title：Applied microbiology and biotechnology

authors： Ulrih NP,Gmajner D,Raspor P

更新日期：2009-08-01 00:00:00

abstract：:Carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (PCB) by Arthrobacter sp. strain B1B. This study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of Aroclor-1242-contaminated soil. Control treatments compare...

journal_title：Applied microbiology and biotechnology

authors： Gilbert ES,Crowley DE

更新日期：1998-10-01 00:00:00

abstract：:Immobilization has been reported as an efficient technique to address the bacterial vulnerability for application in bio self-healing concrete. In this study, for the first time, magnetic iron oxide nanoparticles (IONs) are being practically employed as the protective vehicle for bacteria to evaluate the self-healing ...

journal_title：Applied microbiology and biotechnology

authors： Seifan M,Sarmah AK,Ebrahiminezhad A,Ghasemi Y,Samani AK,Berenjian A

更新日期：2018-03-01 00:00:00

abstract：:Malate synthase (Mls), a key enzyme in the glyoxylate cycle, is required for virulence in microbial pathogens. In this study, we identified the AoMls gene from the nematode-trapping fungus Arthobotrys oligospora. The gene contains 4 introns and encodes a polypeptide of 540 amino acids. To characterize the function of ...

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Wang Y,Zhao Y,Huang Y,Zhang KQ,Yang J

更新日期：2014-03-01 00:00:00

abstract：:The wide application of water-soluble linear polyacrylamides (PAMs) can cause serious environmental pollution. Biological treatment of PAMs receives very limited efficiency due to their recalcitrance to the microbial degradation. Here, we show the bioelectrochemical system (BES) can be used as an effective strategy to...

journal_title：Applied microbiology and biotechnology

authors： Cui YZ,Zhang J,Sun M,Zhai LF

更新日期：2015-01-01 00:00:00

abstract：:Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibiti...

journal_title：Applied microbiology and biotechnology

authors： Hongzhuan Z,Ying T,Xia S,Jinsong G,Zhenhua Z,Beiyu J,Yanyan C,Lulu L,Jue Z,Bing Y,Jing F

更新日期：2020-01-01 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yea...

journal_title：Applied microbiology and biotechnology

authors： Walfridsson M,Anderlund M,Bao X,Hahn-Hägerdal B

更新日期：1997-08-01 00:00:00

abstract：:Arabinofuranose substitutions on xylan are known to interfere with enzymatic hydrolysis of this primary hemicellulose. In this work, two novel α-L-arabinofuranosidases (ABFs), TtABF51A from Thielavia terrestris and EpABF62C from Eupenicillium parvum, were characterized and functionally analyzed. From sequences analyse...

journal_title：Applied microbiology and biotechnology

authors： Long L,Sun L,Lin Q,Ding S,St John FJ

更新日期：2020-10-01 00:00:00

abstract：:We report the successful high-yield expression of Candida utilis uricase in Escherichia coli and the establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein, which was confirmed to be C. utilis uricase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis an...

journal_title：Applied microbiology and biotechnology

authors： Liu X,Wen M,Li J,Zhai F,Ruan J,Zhang L,Li S

更新日期：2011-11-01 00:00:00

abstract：:The TM1072 gene from Thermotoga maritima codifies for a putative form of a rhamnulose-1-phosphate aldolase (Rha-1PA Tm). To investigate this enzyme further, its gene was cloned and expressed in Escherichia coli. The purified enzyme was activated by Co(2+) as a divalent metal ion cofactor, instead of Zn(2+) as its E. c...

journal_title：Applied microbiology and biotechnology

authors： Oroz-Guinea I,Sánchez-Moreno I,Mena M,García-Junceda E

更新日期：2015-04-01 00:00:00

abstract：:Recently, a new gene encoding beta-glucuronidase from Streptococcus equi subsp. zooepidemicus (SEZ) was identified and expressed in Escherichia coli. In this paper, the characterization of the enzyme is described. Specific enzyme activity was 120,000 U/mg purified protein at 37 degrees C and pH = 7.0. The temperature ...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Krahulcová J

更新日期：2007-04-01 00:00:00

abstract：:Empirical approaches to discover anticancer drugs and cancer treatments have made limited progress in the past several decades in finding a cure for cancer. The expanded knowledge of the molecular basis of tumorigenesis and metastasis, together with the inherently vast structural diversity of natural compounds found i...

journal_title：Applied microbiology and biotechnology

authors： Zaidman BZ,Yassin M,Mahajna J,Wasser SP

更新日期：2005-06-01 00:00:00