Production of 10(S)-hydroxy-8(E)-octadecenoic and 7,10(S,S)-hydroxy-8(E)-octadecenoic ethyl esters by Novozym 435 in solvent-free media.

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:D-Tagatose is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and is rarely found in nature. Recently, there has been industrial interest in D-tagatose as a low-calorie sugar-substituting sweetener. This article describes the properties and metabolism of tagatose as well as its commercial impo...

journal_title：Applied microbiology and biotechnology

authors： Kim P

更新日期：2004-08-01 00:00:00

abstract：:Microbial cell factories offer an economic approach for synthesizing "natural'" aromatic flavor compounds. During their fermentation process, the inefficient synthesis pathway and product cytotoxicity are the major barriers to the high-level production. This study combined metabolic engineering and tolerance engineeri...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-12-01 00:00:00

abstract：:A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose conce...

journal_title：Applied microbiology and biotechnology

authors： Santos LO,Gonzales TA,Ubeda BT,Monte Alegre R

更新日期：2007-12-01 00:00:00

abstract：:β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the...

journal_title：Applied microbiology and biotechnology

authors： Parhiz H,Ketcham SA,Zou G,Ghosh B,Fratz-Berilla EJ,Ashraf M,Ju T,Madhavarao CN

更新日期：2019-08-01 00:00:00

abstract：:Production of bacterial cellulose (BC) using sugar cane molasses (MO) with/without the presence of lignosulfonate (MOL) as a sole carbon source in a Hestrin-Schramm medium (HS) was investigated. Six strains of Acetobacter xylinum [American Type Culture Collection 10245 and Institute of Fermentation in Osaka (IFO) 1369...

journal_title：Applied microbiology and biotechnology

authors： Keshk S,Sameshima K

更新日期：2006-09-01 00:00:00

abstract：:Over 9,000 flavonoid compounds have been found in various plants, comprising one of the largest families of natural products. Flavonoids are an essential factor in plant interactions with the environment, often serving as the first line of defense against UV irradiation and pathogen attacks. Flavonoids are also major ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Chen S,Yu O

更新日期：2011-08-01 00:00:00

abstract：:Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable ...

journal_title：Applied microbiology and biotechnology

authors： Renicke C,Taxis C

更新日期：2016-04-01 00:00:00

abstract：:The morbidity and mortality associated with bacterial infections have remained significant despite chemotherapeutic advances. With the emergence of drug-resistant bacterial strains, the situation has become a serious threat to the public health. Thus, there is an urgent need to identify novel antibacterials. The major...

journal_title：Applied microbiology and biotechnology

authors： Akbar N,Siddiqui R,Sagathevan KA,Khan NA

更新日期：2019-05-01 00:00:00

abstract：:2-Hydroxyalkanoates (2HAs) have become the new monomeric constituents of bacterial polyhydroxyalkanoates (PHAs). PHAs containing 2HA monomers, lactate (LA), glycolate (GL), and 2-hydroxybutyrate (2HB) can be synthesized by engineered microbes in which the broad substrate specificities of PHA synthase and propionyl-CoA...

journal_title：Applied microbiology and biotechnology

authors： Matsumoto K,Taguchi S

更新日期：2013-09-01 00:00:00

abstract：:With a cell concentration of 125 g dry biomass l-1 and a dilution rate of 0.1 h-1, Propionibacterium acidipropionici produces 30 g propionic acid l-1 from sugar with a productivity of 3 g l-1 h-1. The yield of propionic acid is approx. 0.36-0.45 g propionic acid g-1 sucrose and is independent of the dilution rate and ...

journal_title：Applied microbiology and biotechnology

authors： Quesada-Chanto A,Afschar AS,Wagner F

更新日期：1994-06-01 00:00:00

abstract：:Sulfate (SO42-) is an often-utilized and well-understood inorganic sulfur source in microorganism culture. Recently, another inorganic sulfur source, thiosulfate (S2O32-), was proposed to be more advantageous in microbial growth and biotechnological applications. Although its assimilation pathway is known to depend on...

journal_title：Applied microbiology and biotechnology

authors： Kawano Y,Onishi F,Shiroyama M,Miura M,Tanaka N,Oshiro S,Nonaka G,Nakanishi T,Ohtsu I

更新日期：2017-09-01 00:00:00

abstract：:Understanding of the effects of perturbation strategies on soil microorganisms is helpful in optimizing bioremediation systems and enhancing their efficiency. Four soil mesocosms were constructed for bioremediation of highly polycyclic aromatic hydrocarbon-contaminated soil using the flowering plant Orychophragmus vio...

journal_title：Applied microbiology and biotechnology

authors： Sun GD,Xu Y,Liu Y,Liu ZP

更新日期：2014-12-01 00:00:00

abstract：:The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These advantages include better source material availability, improved quality control, and reduced concerns about animal virus or prion impurities. A bioengineered hep...

journal_title：Applied microbiology and biotechnology

authors： Wang Z,Yang B,Zhang Z,Ly M,Takieddin M,Mousa S,Liu J,Dordick JS,Linhardt RJ

更新日期：2011-07-01 00:00:00

abstract：:Even though salbutamol (SAL) had remarkable effects on the enhancement of growth rate and carcass composition in different livestock species such as cattle, pigs, sheep and poultry, it was banned as a growth promoter because of its adverse effects on health. However, the specific mechanism by which salbutamol enhances...

journal_title：Applied microbiology and biotechnology

authors： Lu C,Zhou J,Li Y,Zhang D,Wang Z,Li Y,Cheong L,Zhang C,Su X

更新日期：2017-07-01 00:00:00

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:Lactococcus lactis is the most important starter culture organism used in the dairy industry. Although L. lactis species have been awarded Qualified Presumption of Safety status by the European Food Safety Authority, and Generally Regarded as Safe status by the US Food and Drug Administration, some strains can produce...

journal_title：Applied microbiology and biotechnology

authors： del Rio B,Linares DM,Ladero V,Redruello B,Fernández M,Martin MC,Alvarez MA

更新日期：2015-01-01 00:00:00

abstract：:A simple, two-step efficient method to perform multiple-site mutagenesis of a gene from bacterial genome was developed. The method was named polyacrylamide gel electrophoresis (PAGE)-mediated overlap extension polymerase chain reaction (PCR) (POEP). The first step involves synthesis of individual fragments containing ...

journal_title：Applied microbiology and biotechnology

authors： Peng RH,Xiong AS,Yao QH

更新日期：2006-11-01 00:00:00

abstract：:Live attenuated bacteria delivered orally are interesting tools for mucosal immunization. The objective of this study was to construct a novel counter-selection platform based on an attenuated wild-type Escherichia coli (E. coli) strain and to utilize it for the delivery of LTR192G-STaA13Q fusion protein as an oral va...

journal_title：Applied microbiology and biotechnology

authors： Liu W,Yuan C,Bao J,Guan W,Zhao Z,Li X,Tang J,Li D,Shi D

更新日期：2015-01-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:Lantibiotic nukacin ISK-1 is produced by Staphylococcus warneri ISK-1. The dual functional transporter NukT, an ABC transporter maturation and secretion protein, contributes to cleavage of the leader peptide from the prepeptide (modified NukA) and the final transport of nukacin ISK-1. NukT consists of an N-terminal pe...

journal_title：Applied microbiology and biotechnology

authors： Zheng S,Nagao JI,Nishie M,Zendo T,Sonomoto K

更新日期：2018-01-01 00:00:00

abstract：:The main objective of this work was the optimization of the production of the beta-ketolase, acetopyruvate hydrolase, from Pseudomonas putida O1. Orcinol was used as an inducer for enzyme production. The growth medium was optimized in two steps. In the first step, screening for optimal glucose concentration was perfor...

journal_title：Applied microbiology and biotechnology

authors： Hofer H,Mandl T,Steiner W

更新日期：2002-11-01 00:00:00

abstract：:Plant health is determined by the comprehensive effect of soil physicochemical and biological properties. In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plan...

journal_title：Applied microbiology and biotechnology

authors： Meng T,Wang Q,Abbasi P,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:In this study, the kinetic behaviors between n-butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for f...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Hwang ID

更新日期：2006-11-01 00:00:00

abstract：:The rapid global urbanization and other extensive anthropogenic activities exacerbated the worldwide human health risks induced by antibiotic resistance genes (ARGs). Knowledge of the origins and dissemination of ARGs is essential for understanding modern resistome, while little information is known regarding the over...

journal_title：Applied microbiology and biotechnology

authors： Ouyang WY,Huang FY,Zhao Y,Li H,Su JQ

更新日期：2015-07-01 00:00:00

abstract：:Pyruvate carboxylase is an important anaplerotic enzyme replenishing oxaloacetate consumed for biosynthesis during growth, or lysine and glutamic acid production in industrial fermentations. We used regions of homology from pyruvate carboxylase sequences of 12 different species (corresponding to the ATP- and pyruvate-...

journal_title：Applied microbiology and biotechnology

authors： Koffas MA,Ramamoorthi R,Pine WA,Sinskey AJ,Stephanopoulos G

更新日期：1998-09-01 00:00:00

abstract：:In order to achieve efficient homo L-lactic acid fermentation from xylose, we first carried out addition of xylose assimilation ability to Lactococcus lactis IL 1403 by introducing a plasmid carrying the xylRAB genes from L. lactis IO-1 (pXylRAB). Then modification of xylose assimilation pathway was carried out. L. la...

journal_title：Applied microbiology and biotechnology

authors： Shinkawa S,Okano K,Yoshida S,Tanaka T,Ogino C,Fukuda H,Kondo A

更新日期：2011-09-01 00:00:00

abstract：:2-Phenylethanol (2-PE) is an important flavor compound but also impairs cell growth severely, which in turn blocks its bioproduction. However, the molecular mechanism of 2-PE tolerance is unclear. In this study, a superb 2-PE stress-tolerant and producing yeast, Candida glycerinogenes, was selected to uncover the unde...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-05-01 00:00:00

abstract：:There is an error in the Original Publication of this paper for "Acknowledgements" section was missing. ...

journal_title：Applied microbiology and biotechnology

authors： Hauschild P,Röttig A,Madkour MH,Al-Ansari AM,Almakishah NH,Steinbüchel A

更新日期：2020-03-01 00:00:00

abstract：:In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...

journal_title：Applied microbiology and biotechnology

authors： Kim JY,Kim YG,Han YK,Choi HS,Kim YH,Lee GM

更新日期：2011-03-01 00:00:00