Influence of culture conditions on glutathione production by Saccharomyces cerevisiae.

abstract：:Due to the increasing demand for recombinant proteins, the interest in mammalian cell culture, especially of Chinese hamster ovary cells, grows rapidly. This is accompanied by the desire to improve cell lines in order to achieve higher titers and a better product quality. Until recently, most cell line development pro...

journal_title：Applied microbiology and biotechnology

authors： Krämer O,Klausing S,Noll T

更新日期：2010-09-01 00:00:00

abstract：:The aim of this study was to determine the antimicrobial effects of lemongrass essential oil (C. flexuosus) and to determine cytotoxic effects of both test compounds on human dermal fibroblasts. Antimicrobial susceptibility screening was carried out using the disk diffusion method. Antimicrobial resistance was observe...

journal_title：Applied microbiology and biotechnology

authors： Adukwu EC,Bowles M,Edwards-Jones V,Bone H

更新日期：2016-11-01 00:00:00

abstract：:Fossil fuel reserves depletion, global warming, unrelenting population growth, and costly and problematic waste recycling call for renewable resources of energy and consumer products. As an alternative to the 100 % oil economy, production processes based on biomass can be developed. Huge amounts of lignocellulosic was...

journal_title：Applied microbiology and biotechnology

authors： Liguori R,Amore A,Faraco V

更新日期：2013-07-01 00:00:00

abstract：:Excess biomass buildup in biotrickling filters leads to low performance. The effect of biomass accumulation in a biotrickling filter (BTF) packed with polyurethane foam (PUF) was assessed in terms of hydrodynamics and void space availability in a system treating dimethyl disulfide (DMDS) vapors with an alkaliphilic co...

journal_title：Applied microbiology and biotechnology

authors： Arellano-García L,Dorado AD,Morales-Guadarrama A,Sacristan E,Gamisans X,Revah S

更新日期：2015-01-01 00:00:00

abstract：:Microbial biosensors are compact, portable, cost effective, and simple to use, making them seem eminently suitable for the in situ monitoring of environmental pollution. One promising approach for such applications is the fusion of reporter genes with regulatory genes that are dose-dependently responsive to the target...

journal_title：Applied microbiology and biotechnology

authors： Shin HJ

更新日期：2011-02-01 00:00:00

abstract：:Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD 1 and nylE 1 , responsible for 6-aminohexanoate metabolism on the basis of the draft genomic DNA sequence of strain KI72. We amplifie...

journal_title：Applied microbiology and biotechnology

authors： Takehara I,Fujii T,Tanimoto Y,Kato DI,Takeo M,Negoro S

更新日期：2018-01-01 00:00:00

abstract：:A three-dimensional biofilm-electrode reactor (3DBER) was integrated with sulfur autotrophic denitrification (SAD) to improve nitrogen removal performance for wastewater reclamation. The impacts of influent carbon/nitrogen (C/N) ratio, electric current, and hydraulic retention time (HRT) were evaluated. The new proces...

journal_title：Applied microbiology and biotechnology

authors： Hao R,Meng C,Li J

更新日期：2016-08-01 00:00:00

abstract：:Resolution of (R,S)-ibuprofen enantiomers by esterification in different organic solvents was studied using Candida cylindracea lipase. This enzyme preparation had high enantiospecificity for S(+)-ibuprofen in the esterification reaction of a racemic ibuprofen with primary alcohols. The esterification yields of second...

journal_title：Applied microbiology and biotechnology

authors： Mustranta A

更新日期：1992-10-01 00:00:00

abstract：:L-Asparaginases (EC 3.5.1.1) are enzymes that catalyze the hydrolysis of L-asparagine to L-aspartic acid and found in a variety of organisms from microorganisms to mammals. However, they are mainly expressed and produced by microorganisms. Microbial L-asparaginases have received sustained attention due to their irrepl...

journal_title：Applied microbiology and biotechnology

authors： Zuo S,Zhang T,Jiang B,Mu W

更新日期：2015-02-01 00:00:00

abstract：:Nutrition-based strategies to optimize xylose to ethanol conversion by Pichia stipitis were identified in growing and stationary-phase cultures provided with a defined medium varied in nitrogen, vitamin, purine/pyrimidine, and mineral content via full or partial factorial designs. It is surprising to note that station...

journal_title：Applied microbiology and biotechnology

authors： Slininger PJ,Dien BS,Gorsich SW,Liu ZL

更新日期：2006-10-01 00:00:00

abstract：:A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

journal_title：Applied microbiology and biotechnology

authors： Melin ES,Ferguson JF,Puhakka JA

更新日期：1997-06-01 00:00:00

abstract：:Malaria is a widespread and infectious disease that is a leading cause of death in many parts of the world. Eradication of malaria has been a major world health goal for decades, but one that still remains elusive. Other diseases have been eradicated using vaccination, but traditional vaccination methods have thus far...

journal_title：Applied microbiology and biotechnology

authors： Jones CS,Luong T,Hannon M,Tran M,Gregory JA,Shen Z,Briggs SP,Mayfield SP

更新日期：2013-03-01 00:00:00

abstract：:Lactic acid is a versatile organic acid, which finds major application in the food, pharmaceuticals, and chemical industries. Microbial fermentation has the advantage that by choosing a strain of lactic acid bacteria producing only one of the isomers, an optically pure product can be obtained. The production of L: (+)...

journal_title：Applied microbiology and biotechnology

authors： Panesar PS,Kennedy JF,Knill CJ,Kosseva MR

更新日期：2007-02-01 00:00:00

abstract：:Cryparin is an abundant cell-wall-associated hydrophobin of Cryphonectria parasitica. Although cryparin is encoded as a single copy gene, it is the most abundant protein produced by this fungus when grown in liquid culture. Studies to characterize the transcriptional regulatory element(s) found that the fragment betwe...

journal_title：Applied microbiology and biotechnology

authors： Kwon BR,Kim MJ,Park JA,Chung HJ,Kim JM,Park SM,Yun SH,Yang MS,Kim DH

更新日期：2009-05-01 00:00:00

abstract：:The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an optimal C. glutamicum genome, a precise site-directed gene integration method was developed by using a pair of mutant lox sites, one a right element (RE) mutant lox site and the other a left element (LE)...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Inui M,Yukawa H

更新日期：2007-12-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) play an important role in the formation and activity of biofilms in wastewater treatment (WWT). The EPS of the denitrifying biomarker Comamonas denitrificans strain 110, produced in different culture media and growth modes, were characterized. The EPS mainly contained protein (...

journal_title：Applied microbiology and biotechnology

authors： Andersson S,Dalhammar G,Land CJ,Kuttuva Rajarao G

更新日期：2009-03-01 00:00:00

abstract：:Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

journal_title：Applied microbiology and biotechnology

authors： Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

更新日期：2019-04-01 00:00:00

abstract：:Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...

journal_title：Applied microbiology and biotechnology

authors： Zhang Q,Wu ZM,Hao CL,Tang XL,Zheng RC,Zheng YG

更新日期：2019-07-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:Denitrifying sulfide removal (DSR) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. This investigation utilizes a dilution-to-extinction approach at 10(-2) to 10(-6) dilutions to elucidate the c...

journal_title：Applied microbiology and biotechnology

authors： Chen C,Ren N,Wang A,Liu L,Lee DJ

更新日期：2010-03-01 00:00:00

abstract：:Saccharomyces bayanus, being of interest for wine-making, is not as well known as S. cerevisiae and, due to many changes in the yeast classification, accurate data concerning its metabolic activity are difficult to find. In order to produce this yeast as an active dry yeast to be used as a starter in wine-making, its ...

journal_title：Applied microbiology and biotechnology

authors： Serra A,Strehaiano P,Taillandier P

更新日期：2003-10-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative aerobic soil bacterium which can digest crystalline cellulose completely through a strategy different from that of the well-studied cellulolytic aerobic fungi and anaerobic bacteria. However, despite the availability of a published genome sequence, studies on this organism hav...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Ji X,Chen N,Li P,Liu W,Lu X

更新日期：2012-01-01 00:00:00

abstract：:The production of shrimp waste from shrimp processing industries has undergone a dramatic increase in recent years. Continued production of this biomaterial without corresponding development of utilizing technology has resulted in waste collection, disposal, and pollution problems. Currently used chemical process rele...

journal_title：Applied microbiology and biotechnology

authors： Kandra P,Challa MM,Jyothi HK

更新日期：2012-01-01 00:00:00

abstract：:Streptococcus thermophilus (ST) is a lactic acid bacterium widely used in dairy industry and displays several properties which could be beneficial for host. The objective of this study was to investigate, in vitro, the implication of sortase A (SrtA) and sortase-dependent proteins (SDPs) in the adhesion of ST LMD-9 st...

journal_title：Applied microbiology and biotechnology

authors： Kebouchi M,Galia W,Genay M,Soligot C,Lecomte X,Awussi AA,Perrin C,Roux E,Dary-Mourot A,Le Roux Y

更新日期：2016-04-01 00:00:00

abstract：:The effects of copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb) and zinc (Zn) on the biotransformation of organic acids (acetate, propionate and butyrate) and H2 were assessed in serum-bottle microcosms. Experiments were performed over a range of metal concentrations (20-200 mg/l) using biomass from an anaerobic bi...

journal_title：Applied microbiology and biotechnology

authors： Kong IC,Hubbard JS,Jones WJ

更新日期：1994-11-01 00:00:00

abstract：:Nanosized elemental sulfur (ES) is already reported to exert superior antimicrobial efficacy than micron-sized ES, which encourages their use in drugs and therapeutics. The aim of the present study is to explore the possible route and mode of antimicrobial action of orthorhombic (α-SNPs) and monoclinic (β-SNPs) allotr...

journal_title：Applied microbiology and biotechnology

authors： Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

更新日期：2013-07-01 00:00:00

abstract：:The hom-thrB operon (homoserine dehydrogenase/homoserine kinase) and the thrC gene (threonine synthase) of Corynebacterium glutamicum ATCC 13,032 and the homFBR (homoserine dehydrogenase resistant to feedback inhibition by threonine) alone as well as homFBR-thrB operon of C. glutamicum DM 368-3 were cloned separately ...

journal_title：Applied microbiology and biotechnology

authors： Eikmanns BJ,Metzger M,Reinscheid D,Kircher M,Sahm H

更新日期：1991-02-01 00:00:00

abstract：:Acrylamide is an important bulk chemical used for producing polyacrylamide, which is widely applied in diverse fields, such as enhanced oil recovery and water treatment. Acrylamide production with a superior biocatalyst, free-resting Rhodococcus cells containing nitrile hydratase (NHase), has been proven to be simple ...

journal_title：Applied microbiology and biotechnology

authors： Jiao S,Li F,Yu H,Shen Z

更新日期：2020-02-01 00:00:00

abstract：:Production of gluconic acid by cells of Aspergillus niger immobilized on polyurethane foam was studied in repeated-batch shake-flask and bubble-column fermentations. For passive immobilization, various amounts of polyurethane foam and spore suspension were tested in order to obtain a suitable combination for optimal c...

journal_title：Applied microbiology and biotechnology

authors： Vassilev NB,Vassileva MC,Spassova DI

更新日期：1993-06-01 00:00:00