Amplification of three threonine biosynthesis genes in Corynebacterium glutamicum and its influence on carbon flux in different strains.

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As ...

journal_title：Applied microbiology and biotechnology

authors： Johnigk SA,Ecke F,Poehling M,Ehlers RU

更新日期：2004-06-01 00:00:00

abstract：:Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi ( Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus an...

journal_title：Applied microbiology and biotechnology

authors： Aggelis G,Ehaliotis C,Nerud F,Stoychev I,Lyberatos G,Zervakis GI

更新日期：2002-07-01 00:00:00

abstract：:Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

journal_title：Applied microbiology and biotechnology

authors： Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

更新日期：2001-07-01 00:00:00

abstract：:The accumulation of toxic inorganic nitrogen is one of the major water quality problems in intensive aquaculture systems, thus the N removal in aquaculture systems is an important issue for the sustainable development of aquaculture. To understand one of the major microbial N removal processes, anaerobic ammonium oxid...

journal_title：Applied microbiology and biotechnology

authors： Li M,Gu JD

更新日期：2016-10-01 00:00:00

abstract：:Betalamic acid is the structural unit of all the natural pigments betalains. These are nitrogen-containing water-soluble compounds with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales. The formation of betalamic acid from the precursor amino acid 3,4-dihydroxy-L-phenylalani...

journal_title：Applied microbiology and biotechnology

authors： Gandía-Herrero F,García-Carmona F

更新日期：2014-02-01 00:00:00

abstract：:Promoters of sorbose dehydrogenase gene sdh and sorbosone dehydrogenase gene sndh (Psdh and Psndh) in Ketogulonicigenium vulgare DSM 4025 were identified. The transcription initiation site (TIS) of Psdh was guanine 74 bp upstream of the start codon of sdh and the TIS of Psndh was adenine 113 bp upstream of the first c...

journal_title：Applied microbiology and biotechnology

authors： Fu S,Zhang W,Guo A,Wang J

更新日期：2007-07-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) are storage polymers accumulated by numerous prokaryotes in form of intracellular granules. Native PHA granules are formed by amorphous polymer which reveals considerably higher elasticity and flexibility as compared to crystalline pure PHA polymers. The fact that bacteria store PHA in amor...

journal_title：Applied microbiology and biotechnology

authors： Sedlacek P,Slaninova E,Enev V,Koller M,Nebesarova J,Marova I,Hrubanova K,Krzyzanek V,Samek O,Obruca S

更新日期：2019-02-01 00:00:00

abstract：:Red koji or red mold rice is prepared by growing a genus Monascus on steamed rice. For centuries, it has been used in Asia for the production of fermented foods including red rice wine and fermented tofu. Although red koji is an important source of various hydrolytic enzymes critical for food fermentation, information...

journal_title：Applied microbiology and biotechnology

authors： Yasuda M,Tachibana S,Kuba-Miyara M

更新日期：2012-10-01 00:00:00

abstract：:Even though salbutamol (SAL) had remarkable effects on the enhancement of growth rate and carcass composition in different livestock species such as cattle, pigs, sheep and poultry, it was banned as a growth promoter because of its adverse effects on health. However, the specific mechanism by which salbutamol enhances...

journal_title：Applied microbiology and biotechnology

authors： Lu C,Zhou J,Li Y,Zhang D,Wang Z,Li Y,Cheong L,Zhang C,Su X

更新日期：2017-07-01 00:00:00

abstract：:Psc-AFP, isolated from the seeds of Psoralea corylifolia L., is an antimicrobial protein with trypsin inhibitor activity. Its encoding gene was cloned by 3'- rapid amplification of cDNA ends (RACE) combined with Y-shaped adaptor-dependent extension (YADE) method. The gene Psc-AFP encodes a protein of 203 amino acids w...

journal_title：Applied microbiology and biotechnology

authors： Luo XM,Xie CJ,Wang,Wei YM,Cai J,Cheng SS,Yang X-,Sui A-

更新日期：2017-02-01 00:00:00

abstract：:Rhodococcus erythropolis U23A is a polychlorinated biphenyl (PCB)-degrading bacterium isolated from the rhizosphere of plants grown on a PCB-contaminated soil. Strain U23A bphA exhibited 99% identity with bphA1 of Rhodococcus globerulus P6. We grew Arabidopsis thaliana in a hydroponic axenic system, collected, and con...

journal_title：Applied microbiology and biotechnology

authors： Toussaint JP,Pham TT,Barriault D,Sylvestre M

更新日期：2012-09-01 00:00:00

abstract：:Overuse of broad-spectrum antibiotics to control human and plant pathogens greatly accelerated the development of antibiotic resistance among bacteria and fungi. Therefore, usage of new approaches is necessary to control outbreaks of phytopathogenic diseases as well as multidrug-resistant human pathogens. Many of the ...

journal_title：Applied microbiology and biotechnology

authors： Olishevska S,Nickzad A,Déziel E

更新日期：2019-02-01 00:00:00

abstract：:This research investigated the effect of microwave irradiation (MWI) on cell disintegration in municipal secondary sludge (MSS). A representative MSS Gram-positive bacterium (Bacillus subtilis) and Gram-negative bacteria (Acinetobacter calcoaceticus and Pseudomonas aeruginosa) were pure cultured separately and treated...

journal_title：Applied microbiology and biotechnology

authors： Zhou BW,Shin SG,Hwang K,Ahn JH,Hwang S

更新日期：2010-06-01 00:00:00

abstract：:Stereoselective carbon-carbon bond formation with aldolases has become an indispensable tool in preparative synthetic chemistry. In particular, the dihydroxyacetone phosphate (DHAP)-dependent aldolases are attractive because four different types are available that allow access to a complete set of diastereomers of vic...

journal_title：Applied microbiology and biotechnology

authors： Schümperli M,Pellaux R,Panke S

更新日期：2007-05-01 00:00:00

abstract：:The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 ...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Machaku D,Wei P,Huang L,Cai J,Xu Z

更新日期：2017-06-01 00:00:00

abstract：:The interest in industrial biotechnology and its importance opens up challenging possibilities of research in this area. Surfactants have long been among the most versatile of process chemicals. Their market is extremely competitive and manufacturers will have to expand their arsenal to develop products for the year 2...

journal_title：Applied microbiology and biotechnology

authors： Cameotra SS,Makkar RS

更新日期：1998-11-01 00:00:00

abstract：:Population dynamics was studied in a 52-l biotrickling filter (BTF) operated for 182 days and used to clean air contaminated with styrene vapors. In the BTF, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. PCR-amplified 16S rDNA fragments from planktonic and sessile cells within...

journal_title：Applied microbiology and biotechnology

authors： Tresse O,Lorrain MJ,Rho D

更新日期：2002-08-01 00:00:00

abstract：:One solution to the global crisis of antibiotic resistance is the discovery of novel antimicrobial compounds for clinical application. Marine organisms are an attractive and, as yet, relatively untapped resource of new natural products. Cell extracts from the marine diatom, Phaeodactylum tricornutum, have antibacteria...

journal_title：Applied microbiology and biotechnology

authors： Desbois AP,Lebl T,Yan L,Smith VJ

更新日期：2008-12-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:Microbial transglutaminase (mTGase) is commonly known in the food industry as meat glue due to its incredible ability to "glue" meat proteins together. Aside from being widely exploited in the meat processing industries, mTGase is also widely applied in other food and textile industries by catalysing the formation of ...

journal_title：Applied microbiology and biotechnology

authors： Chan SK,Lim TS

更新日期：2019-04-01 00:00:00

abstract：:In this study, eight commercially available, chemically defined Chinese hamster ovary (CHO) cell culture media from different vendors were evaluated in batch culture using an IgG-producing CHO DG44 cell line as a model. Medium adaptation revealed that the occurrence of even small aggregates might be a good indicator o...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Damjanovic L,Kaisermayer C,Kunert R

更新日期：2015-06-01 00:00:00

abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

journal_title：Applied microbiology and biotechnology

authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

更新日期：2016-07-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:Chlorogenate hydrolase (EC 3.1.1.42, CHase) was highly induced in mycelia of Aspergillus sojae AKU 3312 grown in Czapek medium containing either instant coffee powder or coffee pulp as inducer. No CHase formation was observed in the mycelia when cultivated without the inducer. CHase was purified readily from CHase-ind...

journal_title：Applied microbiology and biotechnology

authors： Adachi O,Ano Y,Akakabe Y,Shinagawa E,Matsushita K

更新日期：2008-11-01 00:00:00

abstract：:We report the study of the dynamics of substrate utilization by the genetic modified strain Yarrowia lipolytica H222-S4(p67ICL1) T5. In contrast to its wild-type equivalent, this recombinant strain is able to excrete the sucrose cleaving enzyme invertase. Both the sucrose degradation rate and the glucose and fructose ...

journal_title：Applied microbiology and biotechnology

authors： Moeller L,Zehnsdorf A,Aurich A,Bley T,Strehlitz B

更新日期：2012-02-01 00:00:00

abstract：:Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-guided synthetic transcription a...

journal_title：Applied microbiology and biotechnology

authors： Schüller A,Wolansky L,Berger H,Studt L,Gacek-Matthews A,Sulyok M,Strauss J

更新日期：2020-11-01 00:00:00

abstract：:Five yeast strains, Saccharomyces cerevisiae D8, M12, and S13; Hanseniaspora uvarum S6; and Issatchenkia orientalis KMBL5774, isolated from Korean grapes, were entrapped in Ca-alginate beads, which are non-toxic, simple to use, and economical. Ca-alginate beads containing yeast cells were soaked in protective solution...

journal_title：Applied microbiology and biotechnology

authors： Kim DH,Lee SB,Park HD

更新日期：2017-01-01 00:00:00

abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...

journal_title：Applied microbiology and biotechnology

authors： Ehrenreich A

更新日期：2006-11-01 00:00:00

abstract：:Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical mice...

journal_title：Applied microbiology and biotechnology

authors： Mohanty S,Mukherji S

更新日期：2012-04-01 00:00:00