Biosynthesis of isocitric acid in repeated-batch culture and testing of its stress-protective activity.

abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

journal_title：Applied microbiology and biotechnology

authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

更新日期：2012-01-01 00:00:00

abstract：:A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothri...

journal_title：Applied microbiology and biotechnology

authors： Ying SH,Feng MG

更新日期：2006-08-01 00:00:00

abstract：:Cheese whey (CW), the liquid resulting from the precipitation and removal of milk casein during cheese-making, and the second cheese whey (SCW) derived from the production of cottage and ricotta cheeses are the main byproducts of dairy industry. The major constituent of CW and SCW is lactose, contributing to the high ...

journal_title：Applied microbiology and biotechnology

authors： Zotta T,Solieri L,Iacumin L,Picozzi C,Gullo M

更新日期：2020-04-01 00:00:00

abstract：:Short interfering RNA (siRNA) is one of the members of the family of RNA interference (RNAi). Coupled with the RNA-induced silencing complex (RISC), siRNA is able to trigger the cleavage of target RNAs which serve as a defensive system against pathogens. Meanwhile, siRNA in gene silencing opens a new avenue for the tr...

journal_title：Applied microbiology and biotechnology

authors： Tam C,Wong JH,Cheung RCF,Zuo T,Ng TB

更新日期：2017-10-01 00:00:00

abstract：:Potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. Potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan I type pectin which has previously been shown to ...

journal_title：Applied microbiology and biotechnology

authors： Thomassen LV,Vigsnæs LK,Licht TR,Mikkelsen JD,Meyer AS

更新日期：2011-05-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Inonotus obliquus (Fr.) Pilat is a white rot fungus belonging to the family Hymenochaetaceae in the Basidiomycota. In nature, this fungus rarely forms a fruiting body but usually an irregular shape of sclerotial conk called 'Chaga'. Characteristically, I. obliquus produces massive melanins released to the surface of C...

journal_title：Applied microbiology and biotechnology

authors： Zheng W,Miao K,Liu Y,Zhao Y,Zhang M,Pan S,Dai Y

更新日期：2010-07-01 00:00:00

abstract：:Heparin is a highly sulfated polysaccharide which belongs to the family of glycosaminoglycans. It is involved in various important biological activities. The major biological purpose is the inhibition of the coagulation cascade to maintain the blood flow in the vasculature. These properties are employed in several the...

journal_title：Applied microbiology and biotechnology

authors： Bolten SN,Rinas U,Scheper T

更新日期：2018-10-01 00:00:00

abstract：:India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleu...

journal_title：Applied microbiology and biotechnology

authors： Hemaiswarya S,Raja R,Carvalho IS,Ravikumar R,Zambare V,Barh D

更新日期：2012-12-01 00:00:00

abstract：:Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals L: -(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over th...

journal_title：Applied microbiology and biotechnology

authors： Meussen BJ,de Graaff LH,Sanders JP,Weusthuis RA

更新日期：2012-05-01 00:00:00

abstract：:Enzymes are natural catalysts highly specific to the substrate type and operate under mild conditions of temperature, pressure, and pH with high conversion rates, which makes them more efficient than conventional chemical catalysts. The enzymes can be obtained from various sources, animal, vegetable, and microbiologic...

journal_title：Applied microbiology and biotechnology

authors： Filho DG,Silva AG,Guidini CZ

更新日期：2019-09-01 00:00:00

abstract：:An immobilized-cell biofilm reactor was used for the continuous production of lactic acid by Lactobacillus casei subsp. rhamnosus (ATCC 11443). At Iowa State University, a unique plastic composite support (PCS) that stimulates biofilm formation has been developed. The optimized PCS blend for Lactobacillus contains 50%...

journal_title：Applied microbiology and biotechnology

authors： Cotton JC,Pometto AL 3rd,Gvozdenovic-Jeremic J

更新日期：2001-12-01 00:00:00

abstract：:Pseudomonas strain CT14 was isolated from activated sludge. Strain CT14 contained a 55, 216 bp plasmid that was characterized by sequence analysis. The plasmid had a modular structure with 51 open reading frames (ORFs) that were distributed between two clearly demarcated domains. Domain I primarily contained genes for...

journal_title：Applied microbiology and biotechnology

authors： Bramucci M,Chen M,Nagarajan V

更新日期：2006-06-01 00:00:00

abstract：:This paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in Corynebacterium glutamicum. In the presence of 8.5 mM phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. In addit...

journal_title：Applied microbiology and biotechnology

authors： Lee SY,Kim YH,Min J

更新日期：2010-01-01 00:00:00

abstract：:The thioredoxin system plays a critical role in maintaining the cytoplasm redox state, participating in functions that are important to the cellular viability of fungi. Although functional and structural information on targets in human pathogenic fungi has been scarcely described in the literature, such studies are es...

journal_title：Applied microbiology and biotechnology

authors： Godoy JS,Kioshima ÉS,Abadio AK,Felipe MS,de Freitas SM,Svidzinski TI

更新日期：2016-05-01 00:00:00

abstract：:The construction of a new phagemid vector for display of peptides on the pVIII major coat protein of filamentous bacteriophage is described, in which expression of pVIII-peptide fusions was placed under the control of the arabinose-inducible P(BAD) promoter. The new phagemid showed excellent capacity for the regulatio...

journal_title：Applied microbiology and biotechnology

authors： Fagerlund A,Myrset AH,Kulseth MA

更新日期：2008-10-01 00:00:00

abstract：:Corynebacterium glutamicum ORF NCgl0328, designated noxA, encodes an NADH oxidase enzyme. The noxA gene, which was preferentially expressed in the log growth phase, was found to be under the control of the whcA, whcB, and whcE genes, which play regulatory roles in cells under oxidative stress. While noxA transcription...

journal_title：Applied microbiology and biotechnology

authors： Park JC,Kim Y,Lee HS

更新日期：2015-02-01 00:00:00

abstract：:Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

journal_title：Applied microbiology and biotechnology

authors： Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

更新日期：2007-07-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:Anode properties are critical for the performance of microbial electrolysis cells (MECs). In the present study, Fe nanoparticle-modified graphite disks were used as anodes to investigate the effects of nanoparticles on the performance of Shewanella oneidensis MR-1 in MECs. Results demonstrated that the average current...

journal_title：Applied microbiology and biotechnology

authors： Xu S,Liu H,Fan Y,Schaller R,Jiao J,Chaplen F

更新日期：2012-01-01 00:00:00

abstract：:There is currently an increasing number of cytochrome P450 (CYP450) monooxygenase encoding genes becoming available from various genome-sequencing projects. These enzymes require association with cytochrome P450 reductase (CPR) to achieve optimal activities. In this study, the CYP53B1 gene, which encodes a benzoate pa...

journal_title：Applied microbiology and biotechnology

authors： Shiningavamwe A,Obiero G,Albertyn J,Nicaud JM,Smit M

更新日期：2006-09-01 00:00:00

abstract：:Fidaxomicin, an 18-membered macrolide antibiotic, is highly active against Clostridium difficile, the most common cause of diarrhea in hospitalized patients. Though the biosynthetic mechanism of fidaxomicin has been well studied, little is known about its regulatory mechanism. Here, we reported that FadR1, a LAL famil...

journal_title：Applied microbiology and biotechnology

authors： Li YP,Yu P,Li JF,Tang YL,Bu QT,Mao XM,Li YQ

更新日期：2019-09-01 00:00:00

abstract：:An enzyme preparation from the thermophilic fungus Humicola insolens, Ultraflo L, was able to solubilise more than half of the biomass of brewer's grain and wheat bran, two agro-industrial co-products. While almost all of the ferulic acid was released in the free form, the majority of diferulates were released still a...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

更新日期：2004-06-01 00:00:00

abstract：:Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4)....

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Wang Z,Li W,Zhuang B,Qi H

更新日期：2008-02-01 00:00:00

abstract：:Phytoplankton blooms are predictable features of marine and freshwater habitats. Despite a good knowledge base of the environmental factors controlling blooms, complex interactions between the bacterial and archaeal communities and phytoplankton bloom taxa are only now emerging. Here, the current research on bacterial...

journal_title：Applied microbiology and biotechnology

authors： Song L

更新日期：2017-06-01 00:00:00

abstract：:Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

journal_title：Applied microbiology and biotechnology

authors： Peirotén Á,Landete JM

更新日期：2020-05-01 00:00:00

abstract：:Lactobacillus plantarum MA2 was isolated from traditional Chinese Tibet kefir grains, which possess several excellent properties and functions. We previously demonstrated the antioxidant activities of this bacterium in vitro. However, the maintenance and survival of L. plantarum MA2 inside the murine intestinal tract,...

journal_title：Applied microbiology and biotechnology

authors： Tang W,Xing Z,Hu W,Li C,Wang J,Wang Y

更新日期：2016-08-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:Tributyl phosphate (TBP) is a toxic organophosphorous compound widely used in nuclear fuel processing and chemical industries. Rhodopseudomonas palustris, one of the most metabolically versatile photosynthetic bacteria, is shown here to degrade TBP efficiently under photosynthetic conditions. This study shows that thi...

journal_title：Applied microbiology and biotechnology

authors： Berne C,Pignol D,Lavergne J,Garcia D

更新日期：2007-11-01 00:00:00

abstract：:The influence of low temperature (5-29 degrees C) on the methanogenic activity of non-adapted digested sewage sludge and on temperature/leachate-adapted biomass was assayed by using municipal landfill leachate, intermediates of anaerobic degradation (propionate) and methane precursors (acetate, H2/CO2) as substrates. ...

journal_title：Applied microbiology and biotechnology

authors： Kettunen RH,Rintala JA

更新日期：1997-10-01 00:00:00