Abstract:
:The influence of low temperature (5-29 degrees C) on the methanogenic activity of non-adapted digested sewage sludge and on temperature/leachate-adapted biomass was assayed by using municipal landfill leachate, intermediates of anaerobic degradation (propionate) and methane precursors (acetate, H2/CO2) as substrates. The temperature dependence of methanogenic activity could be described by Arrhenius-derived models. However, both substrate and adaptation affected the temperature dependence. The adaptation of biomass in a leachate-fed upflow anaerobic sludge-blanket reactor at approximately 20 degrees C for 4 months resulted in a sevenfold and fivefold increase of methanogenic activity at 11 degrees C and 22 degrees C respectively. Both acetate and H2/CO2 were methanized even at 5 degrees C. At 22 degrees C, methanogenic activities (acetate 4.8-84 mM) were 1.6-5.2 times higher than those at 11 degrees C. The half-velocity constant (Ks) of acetate utilization at 11 degrees C was one-third of that at 22 degrees C while a similar Ki was obtained at both temperatures. With propionate (1.1-5.5 mM) as substrate, methanogenic activities at 11 degrees C were half those at 22 degrees C. Furthermore, the residual concentration of the substrates was not dependent on temperature. The results suggest that the adaptation of biomass enables the achievement of a high treatment capacity in the anaerobic process even under psychrophilic conditions.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Kettunen RH,Rintala JAdoi
10.1007/s002530051098subject
Has Abstractpub_date
1997-10-01 00:00:00pages
570-6issue
4eissn
0175-7598issn
1432-0614journal_volume
48pub_type
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