Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana.

Abstract:

:A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species.

authors

Ying SH,Feng MG

doi

10.1007/s00253-006-0447-x

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

206-210

issue

1

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-006-0447-x

journal_volume

72

pub_type

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