Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches.

abstract：:Three bacterial strains utilizing paracetamol as the sole carbon, nitrogen, and energy source were isolated from a paracetamol-degrading aerobic aggregate, and assigned to species of the genera Stenotrophomonas and Pseudomonas. The Stenotrophomonas species have not included any known paracetamol degraders until now. I...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Hu J,Zhu R,Zhou Q,Chen J

更新日期：2013-04-01 00:00:00

abstract：:Biogranulation is a promising biotechnology developed for wastewater treatment. Biogranules exhibit a matrix microbial structure, and intensive research has shown that extracellular polymeric substances (EPS) are a major component of the biogranule matrix material in both anaerobic and aerobic granules. This paper aim...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Liu Y,Tay JH

更新日期：2004-08-01 00:00:00

abstract：:Exposure to bioaerosols can adversely influence human health through respiratory tract, eye, and skin irritation. Bioaerosol composition is unique on the International Space Station (ISS), where the size distribution of particles in the air differs from those on Earth. This is due to the lack of gravitational settling...

journal_title：Applied microbiology and biotechnology

authors： Haines SR,Bope A,Horack JM,Meyer ME,Dannemiller KC

更新日期：2019-09-01 00:00:00

abstract：:Polygalacturonases (PG) have evolved in the past years from a pectinase "simply" being used for food processing to an important parameter in plant-fungal interaction. PG-inhibiting proteins (PGIP) that are synthesised in plants as a specific response to PGs of pathogenic fungi, have become a focus as a possible target...

journal_title：Applied microbiology and biotechnology

authors： Lang C,Dörnenburg H

更新日期：2000-04-01 00:00:00

abstract：:The phenomenon of the cell density effect is not readily explained by an obvious nutrient limitation, and a recent study has suggested that for recombinant Autographa californica multiple nucleopolyhedrovirus (rAcMNPV)-infected Sf9 cells, a drop in messenger RNA (mRNA) levels may be sufficient to explain the cell dens...

journal_title：Applied microbiology and biotechnology

authors： Huynh HT,Tran TT,Chan LC,Nielsen LK,Reid S

更新日期：2015-02-01 00:00:00

abstract：:The use of antibiotics may accelerate the development of antibiotic resistance genes (ARGs) and bacteria which shade health risks to humans and animals. The emerging of ARGs in the water environment is becoming an increasing worldwide concern. Hundreds of various ARGs encoding resistance to a broad range of antibiotic...

journal_title：Applied microbiology and biotechnology

authors： Zhang XX,Zhang T,Fang HH

更新日期：2009-03-01 00:00:00

abstract：:Rhodococcus erythropolis cells contain a large set of enzymes that allow them to carry out an enormous number of bioconversions and degradations. Oxidations, dehydrogenations, epoxidations, hydrolysis, hydroxylations, dehalogenations and desulfurisations have been reported to be performed by R. erythropolis cells or e...

journal_title：Applied microbiology and biotechnology

authors： de Carvalho CC,da Fonseca MM

更新日期：2005-06-01 00:00:00

abstract：:Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and ...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Shi J,Gao Z,Yangwu R,Jiang H,Che J,Liu Y

更新日期：2015-06-01 00:00:00

abstract：:This study aimed to model the inactivation of Lactobacillus brevis DSM 6235 while retaining the viability of yeasts during washing brewer's yeast with phosphoric acid and chlorine dioxide. The independent variables in the acid washing were pH (1-3) and temperature (1-9 °C), whereas in the washing with chlorine dioxide...

journal_title：Applied microbiology and biotechnology

authors： Munford ARG,Chaves RD,Granato D,Sant'Ana AS

更新日期：2020-05-01 00:00:00

abstract：:The novel influenza A virus (IAV) defective interfering particle "OP7" inhibits IAV replication in a co-infection and was previously suggested as a promising antiviral agent. Here, we report a batch-mode cell culture-based production process for OP7. In the present study, a seed virus containing standard virus (STV) a...

journal_title：Applied microbiology and biotechnology

authors： Hein MD,Kollmus H,Marichal-Gallardo P,Püttker S,Benndorf D,Genzel Y,Schughart K,Kupke SY,Reichl U

更新日期：2021-01-01 00:00:00

abstract：INTRODUCTION:Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase. M...

journal_title：Applied microbiology and biotechnology

authors： Kroll J,Klinter S,Steinbüchel A

更新日期：2011-02-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:The recent use of photosynthetic organisms such as Chlamydomonas reinhardtii in biomedical applications has demonstrated their potential for the treatment of acute and chronic tissue hypoxia. Moreover, transgenic microalgae have been suggested as an alternative in situ drug delivery system. In this study, we set out t...

journal_title：Applied microbiology and biotechnology

authors： Jarquín-Cordero M,Chávez MN,Centeno-Cerdas C,Bohne AV,Hopfner U,Machens HG,Egaña JT,Nickelsen J

更新日期：2020-01-01 00:00:00

abstract：:Lipase has been used widely in industry. In this study, we have constructed two recombinant Saccharomyces cerevisiae strains displaying two active lipases on the cell surface by cell surface engineering. The genes encoding Yarrowia lipolytica lipases Lip7 and Lip8 were fused with the gene encoding small binding subuni...

journal_title：Applied microbiology and biotechnology

authors： Liu WS,Pan XX,Jia B,Zhao HY,Xu L,Liu Y,Yan YJ

更新日期：2010-10-01 00:00:00

abstract：:The lipids (fats and oils) degradation capabilities of soil microorganisms were investigated for possible application in treatment of lipids-contaminated wastewater. We isolated a strain of the bacterium Raoultella planticola strain 232-2 that is capable of efficiently catabolizing lipids under acidic conditions such ...

journal_title：Applied microbiology and biotechnology

authors： Sugimori D,Watanabe M,Utsue T

更新日期：2013-01-01 00:00:00

abstract：:Triacylglycerols (TAG) and steryl esters (SE) are the principal storage lipids in all eukaryotic cells. In yeasts, these storage lipids accumulate within special organelles known as lipid bodies (LB). In the lipid accumulation-oriented metabolism of the oleaginous yeast Yarrowia lipolytica, storage lipids are mostly f...

journal_title：Applied microbiology and biotechnology

authors： Beopoulos A,Haddouche R,Kabran P,Dulermo T,Chardot T,Nicaud JM

更新日期：2012-02-01 00:00:00

abstract：:Tolypocladium guangdongense is a high-value edible fungus with various medicinal and food safety properties. However, its evolutionary and genetic information is still limited. Mitochondrial genomes are potential models for molecular evolution and phylogenetic studies. In this study, we sequenced the complete mitogeno...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Dai Y,Wang G,Wang C,Gao Y,Deng W,Li T

更新日期：2020-11-01 00:00:00

abstract：:A new highly scalable microbial fuel cell (MFC) design, consisting of a series of cassette electrodes (CE), was examined for increasing power production from organic matter in wastewater. Each CE chamber was composed of a box-shaped flat cathode (two air cathodes on both sides) sandwiched in between two proton-exchang...

journal_title：Applied microbiology and biotechnology

authors： Shimoyama T,Komukai S,Yamazawa A,Ueno Y,Logan BE,Watanabe K

更新日期：2008-08-01 00:00:00

abstract：:The present study reports the successful production of human pre-miR-29b both intra- and extracellularly in the marine phototrophic bacterium Rhodovulum sulfidophilum using recombinant RNA technology. In a first stage, the optimal transformation conditions (0.025 μg of plasmid DNA, with a heat-shock of 2 min at 35 °C)...

journal_title：Applied microbiology and biotechnology

authors： Pereira P,Pedro AQ,Tomás J,Maia CJ,Queiroz JA,Figueiras A,Sousa F

更新日期：2016-04-01 00:00:00

abstract：:Gelatin zymography revealed the presence of proteolytic activity in conditioned medium (CM) from a serum-free, non-infected Spodoptera frugiperda, Sf9 insect cell culture. Two peptidase bands at about 49 and 39 kDa were detected and found to be proform and active form of the same enzyme. The 49-kDa form was visible on...

journal_title：Applied microbiology and biotechnology

authors： Lindskog E,Svensson I,Häggström L

更新日期：2006-07-01 00:00:00

abstract：:Preparative-scale fermentation of gallic acid (3,4,5-trihydroxybenzoic acid) (1) with Beauveria sulfurescens ATCC 7159 gave two new glucosidated compounds, 4-(3,4-dihydroxy-6-hydroxymethyl-5-methoxy-tetrahydro-pyran-2-yloxy)-3-hydroxy-5-methoxy-benzoic acid (4), 3-hydroxy-4,5-dimethoxy-benzoic acid 3,4-dihydroxy-6-hyd...

journal_title：Applied microbiology and biotechnology

authors： Hsu FL,Yang LM,Chang SF,Wang LH,Hsu CY,Liu PC,Lin SJ

更新日期：2007-03-01 00:00:00

abstract：:Mitochondrial dysfunction in Saccharomyces cerevisiae was selected as a marker of ion penetration following carbon ion beam (CIB) irradiation. Respiration-deficient mutants were screened. Following confirmation of negligible spontaneous mutation, eight genetically stable S. cerevisiae respiration-deficient mutant stra...

journal_title：Applied microbiology and biotechnology

authors： Guo X,Zhang M,Gao Y,Cao G,Yang Y,Lu D,Li W

更新日期：2019-02-01 00:00:00

abstract：:Phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. We found that the biotechnologically important soil bacterium Corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-h...

journal_title：Applied microbiology and biotechnology

authors： Kallscheuer N,Vogt M,Kappelmann J,Krumbach K,Noack S,Bott M,Marienhagen J

更新日期：2016-02-01 00:00:00

abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

journal_title：Applied microbiology and biotechnology

authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

更新日期：1996-08-01 00:00:00

abstract：:Our previous work showed that there is a limitation in the use of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification systems in dhfr-non-deficient HEK293 cells, as endogenous dhfr may interfere with the amplification process. In the present study, we successfully generated Epstein-Barr virus...

journal_title：Applied microbiology and biotechnology

authors： Park SH,Park JH,Lee JH,Lee HM,Kang YJ,Lee EJ,Shin S,Lee GM,Kim YG

更新日期：2021-01-01 00:00:00

abstract：:The behaviour of dry baker's yeast ( Saccharomyces cerevisiae type II, Sigma) used as biocatalyst without preliminary growth for the synthesis of 2-heptanol from 2-heptanone in a biphasic system is presented. Cells undergo intracellular trehalose consumption with a stoichiometric ethanol production during the first 15...

journal_title：Applied microbiology and biotechnology

authors： Cappaert L,Larroche C

更新日期：2004-06-01 00:00:00

abstract：:Confocal Raman microspectral imaging (CRMI) is an advanced cell-imaging method that maps endogenous molecular compositions with their unique spectral fingerprint indicators. The aim of this work was to provide a visualized understanding of subcellular features of live osteosarcoma cells using a 532-nm laser excitation...

journal_title：Applied microbiology and biotechnology

authors： Li J,Qin J,Zhang X,Wang R,Liang Z,He Q,Wang Z,Wang K,Wang S

更新日期：2019-08-01 00:00:00

abstract：:Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous pr...

journal_title：Applied microbiology and biotechnology

authors： Gu Y,Zheng J,Feng J,Cao M,Gao W,Quan Y,Dang Y,Wang Y,Wang S,Song C

更新日期：2017-05-01 00:00:00

abstract：:The sheep rumen microbial community plays an important role in animal performance and the environment. Few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. A total of 112 healthy Tan sheep were selected and randomly allotted to one of four dietary...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Cao P,Wang L,Zhao Z,Chen Y,Yang Y

更新日期：2017-05-01 00:00:00

abstract：:India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleu...

journal_title：Applied microbiology and biotechnology

authors： Hemaiswarya S,Raja R,Carvalho IS,Ravikumar R,Zambare V,Barh D

更新日期：2012-12-01 00:00:00