Surface display of active lipases Lip7 and Lip8 from Yarrowia lipolytica on Saccharomyces cerevisiae.

abstract：:Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted fo...

journal_title：Applied microbiology and biotechnology

authors： Gajdoš P,Nicaud JM,Rossignol T,Čertík M

更新日期：2015-10-01 00:00:00

abstract：:Protein turnover through de novo synthesis is critical for sustainable cellular functions. We previously found that glucose consumption rate in Corynebacterium glutamicum under anaerobic conditions increased at temperature higher than the upper limit of growth temperature. Here, we showed that production of lactic and...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Ninomiya K,Takahashi K,Tsuge Y

更新日期：2020-05-01 00:00:00

abstract：:Four potential dehydrogenases identified through literature and bioinformatic searches were tested for L-arabonate production from L-arabinose in the yeast Saccharomyces cerevisiae. The most efficient enzyme, annotated as a D-galactose 1-dehydrogenase from the pea root nodule bacterium Rhizobium leguminosarum bv. trif...

journal_title：Applied microbiology and biotechnology

authors： Aro-Kärkkäinen N,Toivari M,Maaheimo H,Ylilauri M,Pentikäinen OT,Andberg M,Oja M,Penttilä M,Wiebe MG,Ruohonen L,Koivula A

更新日期：2014-12-01 00:00:00

abstract：:Ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities...

journal_title：Applied microbiology and biotechnology

authors： Tan JS,Yeo CR,Popovich DG

更新日期：2017-07-01 00:00:00

abstract：:The membraneless bioelectrochemical reactor (Ml-BER) is useful for dark hydrogen fermentation. The effect of the electrochemical reaction on microorganisms in the Ml-BER was investigated using glucose as the substrate and compared with organisms in a membraneless non-bioelectrochemical reactor (Ml-NBER) and bioelectro...

journal_title：Applied microbiology and biotechnology

authors： Sasaki K,Morita M,Sasaki D,Ohmura N,Igarashi Y

更新日期：2013-08-01 00:00:00

abstract：:Enantioselective oxidation of racemic phenyl-1,2-ethanediol into (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1 was demonstrated. It was found that optically active (R)-(-)-mandelic acid (e.e.p > 99.9 %) is produced leaving the other enantiomer (S)-(+)-phenyl-1,2-ethanediol intact. Using f...

journal_title：Applied microbiology and biotechnology

authors： He YC,Ma CL,Zhang X,Li L,Xu JH,Wu MX

更新日期：2013-08-01 00:00:00

abstract：:Grape juice contains about equal amounts of glucose and fructose, but wine strains of Saccharomyces cerevisiae ferment glucose slightly faster than fructose, leading to fructose concentrations that exceed glucose concentrations in the fermenting must. A high fructose/glucose ratio may contribute to sluggish and stuck ...

journal_title：Applied microbiology and biotechnology

authors： Berthels NJ,Cordero Otero RR,Bauer FF,Pretorius IS,Thevelein JM

更新日期：2008-01-01 00:00:00

abstract：:Pseudomonas chlororaphis is a plant-associated bacterium with reported antagonistic activity against different organisms and plant growth-promoting properties. P. chlororaphis possesses exciting biotechnological features shared with another Pseudomonas with a nonpathogenic phenotype. Part of the antagonistic role of P...

journal_title：Applied microbiology and biotechnology

authors： Moreno-Avitia F,Utrilla J,Bolívar F,Nogales J,Escalante A

更新日期：2020-12-01 00:00:00

abstract：:The hom-thrB operon (homoserine dehydrogenase/homoserine kinase) and the thrC gene (threonine synthase) of Corynebacterium glutamicum ATCC 13,032 and the homFBR (homoserine dehydrogenase resistant to feedback inhibition by threonine) alone as well as homFBR-thrB operon of C. glutamicum DM 368-3 were cloned separately ...

journal_title：Applied microbiology and biotechnology

authors： Eikmanns BJ,Metzger M,Reinscheid D,Kircher M,Sahm H

更新日期：1991-02-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:Phytases are important industrial enzymes able to catalyze the release of up to six phosphates from phytate in a stepwise hydrolysis reaction. Phytases are almost exclusively used as a feed supplement. However, phytases are also used in human nutrition, food processing, non-food industrial products, and emerging appli...

journal_title：Applied microbiology and biotechnology

authors： Herrmann KR,Ruff AJ,Infanzón B,Schwaneberg U

更新日期：2019-08-01 00:00:00

abstract：:Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we deve...

journal_title：Applied microbiology and biotechnology

authors： Shishido T,Muraoka M,Ueda M,Seno M,Tanizawa K,Kuroda S,Fukuda H,Kondo A

更新日期：2006-12-01 00:00:00

abstract：:Sugar beet pulp is a natural carbon source composed mainly of pectin and cellulose, which is utilized and degraded by the ascomycete Penicillium purpurogenum. The fungus also grows on and degrades acetylated xylan which lacks cellulose and pectin. Both carbon sources have been used in our laboratory to grow the fungus...

journal_title：Applied microbiology and biotechnology

authors： Navarrete M,Callegari E,Eyzaguirre J

更新日期：2012-01-01 00:00:00

abstract：:This study investigated the effects of vanillin on methanogenesis and rumen fermentation, and the responses of ruminal protein-degrading bacteria to vanillin (at concentrations of 0, 0.76 and 1.52 g/L), essential oils (clove oil, 1 g/L; origanum oil, 0.50 g/L, and peppermint oil, 1 g/L), and quillaja saponin (at conce...

journal_title：Applied microbiology and biotechnology

authors： Patra AK,Yu Z

更新日期：2014-01-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:Fatty acid-binding protein 2 (Fabp2), which is involved in the transport of long-chain fatty acids, is widely studied in mammals. Nevertheless, the role of this protein in teleost fish is mostly unknown. Here, we produced a fabp2-/- zebrafish (KO) animal model. Compared with wild-type zebrafish (WT), KO had a markedly...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Cao X,Fu L,Gao J

更新日期：2020-03-01 00:00:00

abstract：:Evolvulus alsinoides L. is used for preparation of 'Shankhapushpi', an important popular ayurvedic drug that contributes considerably to the improvement of memory power. The improvement is attributed to the presence of furanocoumarin scopoletin, a metabolite with a wide range of biological activities. This report desc...

journal_title：Applied microbiology and biotechnology

authors： Naikawadi VB,Ahire ML,Lahiri A,Nikam TD

更新日期：2016-04-01 00:00:00

abstract：:Endophytic actinobacteria, which exist in the inner tissues of living plants, have attracted increasing attention among taxonomists, ecologists, agronomists, chemists and evolutionary biologists. Numerous studies have indicated that these prolific actinobacteria appear to have a capacity to produce an impressive array...

journal_title：Applied microbiology and biotechnology

authors： Qin S,Xing K,Jiang JH,Xu LH,Li WJ

更新日期：2011-02-01 00:00:00

abstract：:After crude protein of the marine yeast strains maintained in this laboratory was estimated by the method of Kjehldahl, we found that the G7a strain which was identified to be a strain of Cryptococcus aureus according to the routine identification and molecular methods contained high level of protein and could grow on...

journal_title：Applied microbiology and biotechnology

authors： Gao L,Chi Z,Sheng J,Ni X,Wang L

更新日期：2007-12-01 00:00:00

abstract：:Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

journal_title：Applied microbiology and biotechnology

authors： Misra G,Pavlostathis SG,Perdue EM,Araujo R

更新日期：1996-07-01 00:00:00

abstract：:Novel alginate hydrogels with silver nanoparticles (AgNPs) and honey components were produced with the aim to target multidrug-resistant bacterial strains causing nosocomial wound infections. AgNP synthesis was optimized in highly concentrated honey solutions so that a 5-month stable, colloid solution with 50% of hone...

journal_title：Applied microbiology and biotechnology

authors： Stojkovska J,Petrovic P,Jancic I,Milenkovic MT,Obradovic B

更新日期：2019-10-01 00:00:00

abstract：:Biodegradation can achieve complete and cost-effective elimination of aromatic pollutants through harnessing diverse microbial metabolic processes. Aromatics biodegradation plays an important role in environmental cleanup and has been extensively studied since the inception of biodegradation. These studies, however, a...

journal_title：Applied microbiology and biotechnology

authors： Cao B,Nagarajan K,Loh KC

更新日期：2009-11-01 00:00:00

abstract：:Corynebacterium glutamicum ORF NCgl0328, designated noxA, encodes an NADH oxidase enzyme. The noxA gene, which was preferentially expressed in the log growth phase, was found to be under the control of the whcA, whcB, and whcE genes, which play regulatory roles in cells under oxidative stress. While noxA transcription...

journal_title：Applied microbiology and biotechnology

authors： Park JC,Kim Y,Lee HS

更新日期：2015-02-01 00:00:00

abstract：:The ability to produce (R)- or (S)-beta-phenylalanine ethyl ester (3-amino-3-phenylpropionic acid ethyl ester, BPAE) from racemic BPAE through stereoselective hydrolysis was screened for in BPAE-assimilating microorganisms. Sphingobacterium sp. 238C5 and Arthrobacter sp. 219D2 were found to be potential catalysts for ...

journal_title：Applied microbiology and biotechnology

authors： Ogawa J,Mano J,Shimizu S

更新日期：2006-05-01 00:00:00

abstract：:Fungi can grow in extreme habitats, such as natural stone and mineral building materials, sometimes causing deterioration. Efflorescence-concentrated salt deposits-results from water movement through building material; it can damage masonry materials and other bricks. Fungal isolate KUR1, capable of growth on, and dis...

journal_title：Applied microbiology and biotechnology

authors： Masaphy S,Lavi I,Sultz S,Zabari L

更新日期：2014-06-01 00:00:00

abstract：:Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca(2+)]i...

journal_title：Applied microbiology and biotechnology

authors： Awad WA,Smorodchenko A,Hess C,Aschenbach JR,Molnár A,Dublecz K,Khayal B,Pohl EE,Hess M

更新日期：2015-08-01 00:00:00

abstract：:β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the...

journal_title：Applied microbiology and biotechnology

authors： Parhiz H,Ketcham SA,Zou G,Ghosh B,Fratz-Berilla EJ,Ashraf M,Ju T,Madhavarao CN

更新日期：2019-08-01 00:00:00

abstract：:1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metaboli...

journal_title：Applied microbiology and biotechnology

authors： Kanjilal B,Noshadi I,Bautista EJ,Srivastava R,Parnas RS

更新日期：2015-03-01 00:00:00

abstract：:The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

journal_title：Applied microbiology and biotechnology

authors： Lu XM,Lu PZ

更新日期：2014-09-01 00:00:00

abstract：:An immobilized-cell biofilm reactor was used for the continuous production of lactic acid by Lactobacillus casei subsp. rhamnosus (ATCC 11443). At Iowa State University, a unique plastic composite support (PCS) that stimulates biofilm formation has been developed. The optimized PCS blend for Lactobacillus contains 50%...

journal_title：Applied microbiology and biotechnology

authors： Cotton JC,Pometto AL 3rd,Gvozdenovic-Jeremic J

更新日期：2001-12-01 00:00:00