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Acetopyruvate hydrolase production by Pseudomonas putida O1--optimization of batch and fed-batch fermentations.

Abstract：

:The main objective of this work was the optimization of the production of the beta-ketolase, acetopyruvate hydrolase, from Pseudomonas putida O1. Orcinol was used as an inducer for enzyme production. The growth medium was optimized in two steps. In the first step, screening for optimal glucose concentration was performed. In the second step, a central composite design was used to optimize carbon and nitrogen sources in the medium. After this optimization procedure, a medium was obtained which produced seven times more biomass than the initial medium. Acetopyruvate hydrolase enzyme production was optimized by determining the optimal time of feed and amount of orcinol, using statistical methods. In a subsequent step, the maximal orcinol-degradation rate was determined. The results obtained were used to find an optimal feeding profile for enzyme production. By using the optimized fed-batch process, acetopyruvate hydrolase activity was enhanced from 10 units l(-1)to 400 units l(-1), in comparison with previously reported fermentation experiments. Productivity could even be increased by a factor of 75, to a value of 20 units l(-1 )h(-1).

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Hofer H,Mandl T,Steiner W

doi

10.1007/s00253-002-1133-2

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

293-9

issue

3

eissn

0175-7598

issn

1432-0614

journal_volume

60

pub_type

杂志文章

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