Deletion analysis of the superoxide dismutase (sodM) promoter from Aspergillus oryzae.

Abstract:

:The manganese superoxide dismutase gene (sodM) is very highly expressed in Aspergillus oryzae. To elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (GUS) as a reporter. Deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodM promoter caused a drastic decrease in GUS activity. In addition, an electrophoretic gel mobility shift assay (EMSA) implicated a 30-bp element from -209 to -178 containing cis-element(s) in the high-level expression. The results of fine structure deletion analysis of this region were consistent with the EMSA results. To confirm these findings, we constructed enhanced sodM promoters by incorporating tandem repeats of this region, which resulted in an approximate twofold increase in expression relative to the native sodM promoter.

authors

Hisada H,Sano M,Ishida H,Hata Y,Abe Y,Machida M

doi

10.1007/s00253-006-0388-4

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

1048-53

issue

5

eissn

0175-7598

issn

1432-0614

journal_volume

72

pub_type

杂志文章
  • Early stages in biofilm development in methanogenic fluidized-bed reactors.

    abstract::Biofilm development in methanogenic fluidized-bed reactors with sand as the carrier was studied on a laboratory scale. The microorganisms present in consecutive layers of the biofilm of mature sludge granules were preliminarily characterized on the basis of their morphology, element composition and adhesion capacity a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00164535

    authors: Lauwers AM,Heinen W,Gorris LG,van der Drift C

    更新日期:1990-06-01 00:00:00

  • Immunoaffinity layering of enzymes. Stabilization and use in flow injection analysis of glucose and hydrogen peroxide.

    abstract::A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described. Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated by ammoni...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051534

    authors: Farooqi M,Sosnitza P,Saleemuddin M,Ulber R,Scheper T

    更新日期:1999-09-01 00:00:00

  • Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification.

    abstract::Oils and fats are the most important renewable raw materials of the chemical industry. They make available fatty acids in such purity that they may be used for chemical conversions and for the synthesis of chemically pure compounds. Oleic acid (1) from "new sunflower," linoleic acid (2) from soybean, linolenic acid (3...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-006-0335-4

    authors: Metzger JO,Bornscheuer U

    更新日期:2006-06-01 00:00:00

  • Bioflavoring and beer refermentation.

    abstract::Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-003-1340-5

    authors: Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

    更新日期:2003-08-01 00:00:00

  • Aerobic dechlorination of low-chlorinated biphenyls by bacterial biofilms in packed-bed batch bioreactors.

    abstract::Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00578472

    authors: Fava F,Di Gioia D,Marchetti L,Quattroni G

    更新日期:1996-05-01 00:00:00

  • Production of 10(S)-hydroxy-8(E)-octadecenoic and 7,10(S,S)-hydroxy-8(E)-octadecenoic ethyl esters by Novozym 435 in solvent-free media.

    abstract::Novozym 435, lipase B from Candida antarctica, was used in this study for the production of ethyl esters. For the first time, trans-hydroxy-fatty acid ethyl esters were synthesized in vitro in solvent-free media. We studied the effects of the substrate-ethanol molar ratio and enzyme synthetic stability of the biocatal...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5059-7

    authors: Martin-Arjol I,Busquets M,Isbell TA,Manresa A

    更新日期:2013-09-01 00:00:00

  • Effects of oxygen supply on the production of nikkomycin with immobilized cells of Streptomyces tendae.

    abstract::For continuous production of the antibiotic nikkomycin immobilized cells have been used in a fluidized bed bioreactor. Cells of Streptomyces tendae were immobilized on sintered glass particles. Different biomass concentrations on the particles correspond to different thicknesses of mycelial layers because growth occur...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00170913

    authors: Trück HU,Chmiel H,Hammes WP,Trösch W

    更新日期:1990-10-01 00:00:00

  • Heterologous expression, characterization and evaluation of the matrix protein from Newcastle disease virus as a target for antiviral therapies.

    abstract::Newcastle disease virus (NDV) is an infectious agent of a large variety of birds, including chicken, which poses a real threat to the agriculture industry. Matrix (M) proteins of NDV and many other viruses perform critical functions during viral assembly and budding from the host cell. M-proteins are well conserved an...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5043-2

    authors: Iram N,Shah MS,Ismat F,Habib M,Iqbal M,Hasnain SS,Rahman M

    更新日期:2014-02-01 00:00:00

  • Genome shuffling improves pigment and other bioactive compound production in Monascus purpureus.

    abstract::Monascus purpureus, a pigment-producing ascomycetous fungus, has been traditionally used for red rice preparation using solid-state fermentation. The objective of this study was to develop an improved pigment-producing strain of M. purpureus MTCC 1090 through genome shuffling followed by detailed analytical estimation...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10987-0

    authors: Ghosh S,Dam B

    更新日期:2020-12-01 00:00:00

  • A novel cephalosporin deacetylating acetyl xylan esterase from Bacillus subtilis with high activity toward cephalosporin C and 7-aminocephalosporanic acid.

    abstract::A cephalosporin deacetylating acetyl xylan esterase was cloned from the genomic DNA of Bacillus subtilis CICC 20034 and functionally expressed in Escherichia coli. Its gene contained an open reading frame of 957 bp encoding 318 amino acids with a calculated mass of 35,607 Da, and it displayed significant identity to a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5056-x

    authors: Tian Q,Song P,Jiang L,Li S,Huang H

    更新日期:2014-03-01 00:00:00

  • Enrichment of cordycepin for cosmeceutical applications: culture systems and strategies.

    abstract::Cordyceps spp. is the herbal medication initially used in China and has been reported as the unique resource of cordycepin. Cordycepin exhibits many health benefits, including anti-photoaging and anti-pigmentation; therefore, it potentially is a bioactive ingredient of cosmetic products. In order to enrich cordycepin ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-09623-3

    authors: Kunhorm P,Chaicharoenaudomrung N,Noisa P

    更新日期:2019-02-01 00:00:00

  • Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction.

    abstract::When sucrose was used as the carbon source, the Basidiomycete Coprinopsis cinerea showed poor growth and low laccase activity in pure culture, but greatly enhanced the level of laccase activity (>1800 U/L) during coculture with the Mucoromycete Gongronella sp. w5. As a result, the mechanism of laccase overproduction i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9469-4

    authors: Hu J,Zhang Y,Xu Y,Sun Q,Liu J,Fang W,Xiao Y,Kües U,Fang Z

    更新日期:2019-01-01 00:00:00

  • Identification of promoters of two dehydrogenase genes in Ketogulonicigenium vulgare DSM 4025 and their strength comparison in K. vulgare and Escherichia coli.

    abstract::Promoters of sorbose dehydrogenase gene sdh and sorbosone dehydrogenase gene sndh (Psdh and Psndh) in Ketogulonicigenium vulgare DSM 4025 were identified. The transcription initiation site (TIS) of Psdh was guanine 74 bp upstream of the start codon of sdh and the TIS of Psndh was adenine 113 bp upstream of the first c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-0930-z

    authors: Fu S,Zhang W,Guo A,Wang J

    更新日期:2007-07-01 00:00:00

  • Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis.

    abstract::The genes encoding two conjugated polyketone reductases (CPR-C1, CPR-C2) of Candida parapsilosis IFO 0708 were cloned and sequenced. The genes encoded a total of 304 and 307 amino acid residues for CPR-C1 and CPR-C2, respectively. The deduced amino acid sequences of the two enzymes showed high similarity to each other...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1484-3

    authors: Kataoka M,Delacruz-Hidalgo AR,Akond MA,Sakuradani E,Kita K,Shimizu S

    更新日期:2004-04-01 00:00:00

  • Efficient refolding of a recombinant abzyme : structural and catalytic characterizations.

    abstract::Catalytic antibodies are currently being investigated in order to understand their role under physio-pathological situations. To this end, the knowledge of structure-function relationships is of great interest. Recombinant scFv fragments are smaller and easier to genetically manipulate than whole antibodies, making th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4600-4

    authors: Ben Naya R,Matti K,Guellier A,Matagne A,Boquet D,Thomas D,Friboulet A,Avalle B,Padiolleau-Lefèvre S

    更新日期:2013-09-01 00:00:00

  • A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase.

    abstract::Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7850-8

    authors: Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

    更新日期:2016-12-01 00:00:00

  • Glucose consumption rate critically depends on redox state in Corynebacterium glutamicum under oxygen deprivation.

    abstract::Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6540-2

    authors: Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

    更新日期:2015-07-01 00:00:00

  • The quest for microbial reductive dechlorination of C (2) to C (4) chloroalkanes is warranted.

    abstract::C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-002-1174-6

    authors: De Wildeman S,Verstraete W

    更新日期:2003-04-01 00:00:00

  • Utilization of protein-rich residues in biotechnological processes.

    abstract::A drawback of biotechnological processes, where microorganisms convert biomass constituents, such as starch, cellulose, hemicelluloses, lipids, and proteins, into wanted products, is the economic feasibility. Particularly the cost of nitrogen sources in biotechnological processes can make up a large fraction of total ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-015-7278-6

    authors: Pleissner D,Venus J

    更新日期:2016-03-01 00:00:00

  • Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid.

    abstract::An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1280-6

    authors: Yu IS,Yeom SJ,Kim HJ,Lee JK,Kim YH,Oh DK

    更新日期:2008-02-01 00:00:00

  • Genetic analysis of the phosphinothricin-tripeptide biosynthetic pathway of Streptomyces viridochromogenes Tü494.

    abstract::Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

    journal_title:Applied microbiology and biotechnology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1007/BF00169345

    authors: Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

    更新日期:1991-03-01 00:00:00

  • An Indian scenario on renewable and sustainable energy sources with emphasis on algae.

    abstract::India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-012-4487-0

    authors: Hemaiswarya S,Raja R,Carvalho IS,Ravikumar R,Zambare V,Barh D

    更新日期:2012-12-01 00:00:00

  • Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol.

    abstract::Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-0949-1

    authors: Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

    更新日期:2007-07-01 00:00:00

  • rtfA, a putative RNA-Pol II transcription elongation factor gene, is necessary for normal morphological and chemical development in Aspergillus flavus.

    abstract::The filamentous fungus Aspergillus flavus is an agriculturally important opportunistic plant pathogen that produces potent carcinogenic compounds called aflatoxins. We identified the A. flavus rtfA gene, the ortholog of rtf1 in Saccharomyces cerevisiae and rtfA in Aspergillus nidulans. Interestingly, rtfA has multiple...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7418-7

    authors: Lohmar JM,Harris-Coward PY,Cary JW,Dhingra S,Calvo AM

    更新日期:2016-06-01 00:00:00

  • Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor.

    abstract::Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5099-z

    authors: Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

    更新日期:2013-10-01 00:00:00

  • Genetically engineered microbial biosensors for in situ monitoring of environmental pollution.

    abstract::Microbial biosensors are compact, portable, cost effective, and simple to use, making them seem eminently suitable for the in situ monitoring of environmental pollution. One promising approach for such applications is the fusion of reporter genes with regulatory genes that are dose-dependently responsive to the target...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-2990-8

    authors: Shin HJ

    更新日期:2011-02-01 00:00:00

  • Low-serum medium development for human diploid fibroblast microcarrier cultures.

    abstract::A new medium supplement mixture, PPRF92, has been developed to enable the serial subculture of human diploid fibroblasts (MRC-5 cells) on microcarriers. Furthermore, the PPRF92 supplements enable cell growth at serum levels as low as 1%. Through an optimization programme, the PPRF92 supplements have evolved into a sim...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00174462

    authors: Forestell SP,Kalogerakis N,Behie LA

    更新日期:1992-11-01 00:00:00

  • Effects of hydraulic retention time on aerobic granulation and granule growth kinetics at steady state with a fast start-up strategy.

    abstract::A hydraulic retention time (HRT) of 4, 6, and 8 h was employed, respectively, in three reactors to study the effects of HRT on granulation with a newly developed fast granulation strategy, i.e., a strategy by combining strong hydraulic selection pressure with high organic loading rate (OLR). Granules with clear bounda...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6993-3

    authors: Liu YQ,Zhang X,Zhang R,Liu WT,Tay JH

    更新日期:2016-01-01 00:00:00

  • Mining Xanthomonas and Streptomyces genomes for new pectinase-encoding sequences and their heterologous expression in Escherichia coli.

    abstract::Microbial genome sequencing has left a legacy of annotated yet uncharacterized genes or open reading frames, activities that may have useful applications in health and/or the environment. We are interested in the discovery and characterization of potentially new pectinolytic activities for the enzymatic retting of nat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1389-2

    authors: Xiao Z,Boyd J,Grosse S,Beauchemin M,Coupe E,Lau PC

    更新日期:2008-04-01 00:00:00

  • Enhanced leavening properties of baker's yeast by reducing sucrase activity in sweet dough.

    abstract::Leavening ability in sweet dough is required for the commercial applications of baker's yeast. This property depends on many factors, such as glycolytic activity, sucrase activity, and osmotolerance. This study explored the importance of sucrase level on the leavening ability of baker's yeast in sweet dough. Furthermo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7449-0

    authors: Zhang CY,Lin X,Feng B,Liu XE,Bai XW,Xu J,Pi L,Xiao DG

    更新日期:2016-07-01 00:00:00