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Biosynthesis of D-danshensu from L-DOPA using engineered Escherichia coli whole cells.

Abstract：

:D-Danshensu (D-DSS), a traditional Chinese medicine, is used to treat cardiovascular and cerebrovascular diseases. However, current isolation protocols for D-DSS both natural and synthetic are not ideal; therefore, in this study, we have developed a whole-cell biotransformation method to produce D-DSS from L-DOPA. This was done by co-expressing L-amino acid deaminase (aadL), D-lactate dehydrogenase (ldhD), and glucose dehydrogenase (gdh). To begin to optimize the production of D-DSS, varying copy number plasmids were used to express each of the required genes. The resulting strain, Escherichia coli ALG7, which strongly overexpressed aadL, ldhD, and weakly overexpressed gdh, yielded a 378% increase in D-DSS production compared to E. coli ALG1. Furthermore, the optimal reaction conditions for the production of D-DSS were found to be a pH of 7.5, temperature at 35 °C, and 50 g/L wet cells for 12 h. Under these optimized conditions, the D-DSS amount achieved 119.1 mM with an excellent ee (> 99.9%) and a productivity of 9.9 mM/h.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Xiong T,Jiang J,Bai Y,Fan TP,Zhao Y,Zheng X,Cai Y

doi

10.1007/s00253-019-09947-0

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

6097-6105

issue

15

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-019-09947-0

journal_volume

103

pub_type

杂志文章

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