Metal resistance in acidophilic microorganisms and its significance for biotechnologies.

abstract：:The use of surfactin and mycosubtilin as an eco-friendly alternative to control lettuce downy mildew caused by the obligate pathogen Bremia lactucae was investigated. Preliminary ecotoxicity evaluations obtained from three different tests revealed the rather low toxicity of these lipopeptides separately or in combinat...

journal_title：Applied microbiology and biotechnology

authors： Deravel J,Lemière S,Coutte F,Krier F,Van Hese N,Béchet M,Sourdeau N,Höfte M,Leprêtre A,Jacques P

更新日期：2014-01-01 00:00:00

abstract：:Biotin-dependent human acetyl-CoA carboxylases (ACCs) are integral in homeostatic lipid metabolism. By securing posttranslational biotinylation, ACCs perform coordinated catalytic functions allosterically regulated by phosphorylation/dephosphorylation and citrate. The production of authentic recombinant ACCs is heeded...

journal_title：Applied microbiology and biotechnology

authors： Hwang IW,Makishima Y,Kato T,Park S,Terzic A,Park EY

更新日期：2014-10-01 00:00:00

abstract：:The anthropogenic release of toxic metals into the environment poses danger to the health of both humans and the local ecosystem. Biosensors for the detection of metals have been developed to improve our ability to monitor these environmental contaminants, yet most of these sensors use heterotrophic bacterial hosts, w...

journal_title：Applied microbiology and biotechnology

authors： Lacey RF,Ye D,Ruffing AM

更新日期：2019-03-01 00:00:00

abstract：:Intervention strategies for obesity are global issues that require immediate attention. The objective of this study was to assess the possibility that Clostridium butyricum and its potential components could reduce lipogenesis. Co-culture experiments of Caco-2 cells and 1 × 10(6), 1 × 10(7), and 1 × 10(8) CFU/ml of C....

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Guo Y,Liu H,Gao J,Nie W

更新日期：2014-09-01 00:00:00

abstract：:The addition of humic acid (HA) to polycyclic aromatic hydrocarbon (PAH) contaminated systems has been shown to enhance, inhibit, or have no effect on the biodegradation of these PAHs. In this study, the surfactant effects of Elliott soil HA (ESHA) at two pH values were tested. At pH 7.0, ESHA did not behave as a surf...

journal_title：Applied microbiology and biotechnology

authors： Liang Y,Britt DW,McLean JE,Sorensen DL,Sims RC

更新日期：2007-04-01 00:00:00

abstract：:Forty-six pulp-bleaching fungi were screened for production of key enzymes for conversion of polychlorinated dibenzo-p-dioxins--lignin peroxidase (LiP), manganese peroxidase (MnP), and manganese-independent peroxidase (MiP)--under various conditions that would allow their utilization in the environment. Of 38 MnP-prod...

journal_title：Applied microbiology and biotechnology

authors： Manji S,Ishihara A

更新日期：2004-01-01 00:00:00

abstract：:Avian influenza virus (AIV) is spreading worldwide and is a serious threat to the health of poultry and humans. In many countries, low pathogenic AIVs, such as H9N2, have become an enormous economic burden on the commercial poultry industry because they cause mild respiratory disease and decrease egg production. A rec...

journal_title：Applied microbiology and biotechnology

authors： Yang WT,Yang GL,Shi SH,Liu YY,Huang HB,Jiang YL,Wang JZ,Shi CW,Jing YB,Wang CF

更新日期：2017-06-01 00:00:00

abstract：:Wood contains primary extractives, which are present in all woods, and secondary extractives, which are confined in certain wood species. Extractives in wood play a major role in wood-bonding processes, as they can contribute to or determine the bonding relevant properties of wood such as acidity and wettability. Ther...

journal_title：Applied microbiology and biotechnology

authors： Roffael E

更新日期：2016-02-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:Secretion of fusion proteins composed of cytoplasmic protein dihydrofolate reductase (DHFR) and the Escherichia coli alpha-haemolysin (HlyA) C-terminal sequence was examined through the haemolysin secretion machinery of E. coli. DHFR of various lengths was combined with the HlyA C-terminal region, and both secretion a...

journal_title：Applied microbiology and biotechnology

authors： Nakano H,Kawakami Y,Nishimura H

更新日期：1992-09-01 00:00:00

abstract：:Prenyltransferase NovQ is a vital class involved in the biosynthesis of secondary metabolites such as clorobiocin and novobiocin. To investigate the relationship between structure and catalytic properties of NovQ, here, we have analyzed the substrate-binding site, namely PT barrel, and revealed that menadione hydroqui...

journal_title：Applied microbiology and biotechnology

authors： Ni W,Zheng Z,Liu H,Wang P,Wang H,Sun X,Yang Q,Fang Z,Tang H,Zhao G

更新日期：2020-05-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Zearalenone (ZEN) is one of the common mycotoxins with quite high occurrence rate and is harmful to animal and human health. Lactobacillus reuteri is known as a probiotic bacterium with active immune stimulating and high inhibitory activity against pathogenic microorganisms. In this study, we expressed the lactonohydr...

journal_title：Applied microbiology and biotechnology

authors： Liu F,Malaphan W,Xing F,Yu B

更新日期：2019-11-01 00:00:00

abstract：:Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...

journal_title：Applied microbiology and biotechnology

authors： Kobayashi T,Hakamada Y,Hitomi J,Koike K,Ito S

更新日期：1996-03-01 00:00:00

abstract：:The temporal and spatial distribution of the genus Bacillus and Clostridium histolyticum group in swine manure composting was determined by fluorescent in situ hybridization using fluorescently labeled 16S rRNA-targeted oligonucleotide probes LGC353b and Chis150, respectively. The temporal distribution of total bacter...

journal_title：Applied microbiology and biotechnology

authors： Yi J,Zheng R,Li F,Chao Z,Deng CY,Wu J

更新日期：2012-03-01 00:00:00

abstract：:A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...

journal_title：Applied microbiology and biotechnology

authors： Park HY,Kim M,Han J

更新日期：2011-08-01 00:00:00

abstract：:The pyrene-degrading Mycobacterium strain AP1 was found to utilize fluoranthene as a sole source of carbon and energy. Identification of metabolites formed from fluoranthene (by growing cells and washed-cell suspensions), the kinetics of metabolite accumulation, and metabolite-feeding studies all indicated that strain...

journal_title：Applied microbiology and biotechnology

authors： López Z,Vila J,Minguillón C,Grifoll M

更新日期：2006-05-01 00:00:00

abstract：:Baicalin (baicalein 7-O-β-D-glucuronide) is one of the major flavonoid glucuronides found in traditional herbal medicines. Because its aglycone, baicalein, is absorbed more quickly and shows more effective properties than baicalin, the conversion of baicalin into baicalein by β-glucuronidase (GUS) has drawn the attent...

journal_title：Applied microbiology and biotechnology

authors： Sakurama H,Kishino S,Uchibori Y,Yonejima Y,Ashida H,Kita K,Takahashi S,Ogawa J

更新日期：2014-05-01 00:00:00

abstract：:ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA) limits its application in R-amine synthesis. A fluorescence-based TA activity screening system was develo...

journal_title：Applied microbiology and biotechnology

authors： Cheng F,Chen XL,Xiang C,Liu ZQ,Wang YJ,Zheng YG

更新日期：2020-04-01 00:00:00

abstract：:Fatty acid-binding protein 2 (Fabp2), which is involved in the transport of long-chain fatty acids, is widely studied in mammals. Nevertheless, the role of this protein in teleost fish is mostly unknown. Here, we produced a fabp2-/- zebrafish (KO) animal model. Compared with wild-type zebrafish (WT), KO had a markedly...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Cao X,Fu L,Gao J

更新日期：2020-03-01 00:00:00

abstract：:Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) contribute significantly to the nitrogen cycle. The community structure of AOA and AOB is diverse in the different wetlands. Few studies have comparatively investigated the community structure and dynamics of ammonia-oxidizing organisms in the marsh ...

journal_title：Applied microbiology and biotechnology

authors： Gao D,Liu F,Xie Y,Liang H

更新日期：2018-08-01 00:00:00

abstract：:The human intestine hosts various complex microbial communities that are closely associated with multiple health and disease processes. Determining the composition and function of these microbial communities is critical to unveil disease mechanisms and promote human health. Recently, meta-omic strategies have been dev...

journal_title：Applied microbiology and biotechnology

authors： Xiao M,Yang J,Feng Y,Zhu Y,Chai X,Wang Y

更新日期：2017-04-01 00:00:00

abstract：:This research develops a safe, inexpensive, and more accessible source for synthesis of silver nanoparticles. The bioactive silver composites synthesized by Lactococcus lactis 56 KY484989 (LCLB56-AgCs) were characterized by various physico-chemical techniques and investigated for their antimicrobial activity and cytot...

journal_title：Applied microbiology and biotechnology

authors： Viorica RP,Pawel P,Kinga M,Michal Z,Katarzyna R,Boguslaw B

更新日期：2017-10-01 00:00:00

abstract：:Nanobiotechnology raises fascinating possibilities for new analytical assays in various fields such as bioelectronic assembly, biomechanics and sampling techniques, as well as in chips or micromachined devices. Recently, nanotechnology has greatly impacted biotechnological research with its potential applications in s...

journal_title：Applied microbiology and biotechnology

authors： Lee SJ,Lee SY

更新日期：2004-04-01 00:00:00

abstract：:Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. Considering that rich medium favors growth rate and, simultaneously, is...

journal_title：Applied microbiology and biotechnology

authors： Hohnholz R,Pohlmann KJ,Achstetter T

更新日期：2017-12-01 00:00:00

abstract：:The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent. ...

journal_title：Applied microbiology and biotechnology

authors： Carabeo-Pérez A,Guerra-Rivera G,Ramos-Leal M,Jiménez-Hernández J

更新日期：2019-12-01 00:00:00

abstract：:Seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus Neocallimastix frontalis. The fractions (ES1, ES3, ES2U1, ES2U2, ES2U4, ES2U3C1 and ES2U3C2) were separated from each other and from a fraction that could solubilize crystalline cellulose...

journal_title：Applied microbiology and biotechnology

authors： Wood TM,Wilson CA,McCrae SI

更新日期：1995-12-01 00:00:00

abstract：:The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

journal_title：Applied microbiology and biotechnology

authors： Fourage L,Colas B

更新日期：2001-08-01 00:00:00

abstract：:The most notable microbial survival models of disinfection kinetics are the original and modified versions of the static Chick-Watson-Hom's (CWH) initially developed for water chlorination. They can all be viewed as special cases of the Weibull survival model, where the observed static curve is the cumulative form (CD...

journal_title：Applied microbiology and biotechnology

authors： Peleg M

更新日期：2021-01-01 00:00:00

abstract：:The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. The performance of three different inex...

journal_title：Applied microbiology and biotechnology

authors： Sella SR,Guizelini BP,Zanello PH,Vandenberghe LP,Ribeiro CA,Minozzo JC,Soccol CR

更新日期：2012-01-01 00:00:00