Efficient production of deoxynucleoside-5'-monophosphates using deoxynucleoside kinase coupled with a GTP-regeneration system.

abstract：:A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal R...

journal_title：Applied microbiology and biotechnology

authors： Gonzalez-Martinez A,Rodriguez-Sanchez A,Rivadeneyra MA,Rivadeneyra A,Martin-Ramos D,Vahala R,Gonzalez-Lopez J

更新日期：2017-01-01 00:00:00

abstract：:Leuconostoc carnosum 4010 is a protective culture for meat products. It kills the foodborne pathogen Listeria monocytogenes by producing two class IIa (pediocin-like) bacteriocins, leucocin A and leucocin C. The genes for leucocin A production have previously been characterised from Leuconostoc gelidum UAL 187, wherea...

journal_title：Applied microbiology and biotechnology

authors： Wan X,Li R,Saris PE,Takala TM

更新日期：2013-04-01 00:00:00

abstract：:Recombinant DNA technology was used to insert a fetal liver genomic library ApaI fragment encoding for human erythropoietin (Epo) into Bowes melanoma cells. The cells expressed the erythropoietin gene, and Epo was secreted into the culture medium together with the normally-secreted tissue plasminogen activator. Attemp...

journal_title：Applied microbiology and biotechnology

authors： Keay L,Walsh JM,Buchholz RM,Terando JM

更新日期：1990-11-01 00:00:00

abstract：:Bioconversion of cassava-derived glucose to 2-keto-D-gluconic acid (2-KDG) using resting cells of immobilized Pseudomonas aeruginosa IFO 3448 was investigated. The tuberous roots of cassava were selected as the feedstock as they are inexpensive and widely available, and possess high amounts of starch (approximately 70...

journal_title：Applied microbiology and biotechnology

authors： Chia M,Van Nguyen TB,Choi WJ

更新日期：2008-04-01 00:00:00

abstract：:Environmental research delivers valuable bacterial resources for biotechnology. We believe that systematic long-term preservation of bacteria will promote future biotechnological innovations, by safeguarding the accessibility of bacteria already recognized to have interesting features and providing a "pool" of bacteri...

journal_title：Applied microbiology and biotechnology

authors： Heylen K,Hoefman S,Vekeman B,Peiren J,De Vos P

更新日期：2012-05-01 00:00:00

abstract：:Nitrate, injected into oil fields, can oxidize sulfide formed by sulfate-reducing bacteria (SRB) through the action of nitrate-reducing sulfide-oxidizing bacteria (NR-SOB). When reservoir rock contains siderite (FeCO(3)), the sulfide formed is immobilized as iron sulfide minerals, e.g. mackinawite (FeS). The aim of ou...

journal_title：Applied microbiology and biotechnology

authors： Lin S,Krause F,Voordouw G

更新日期：2009-05-01 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00

abstract：:Staphylococcus aureus is a pathogen responsible for severe community- and nosocomially acquired infections. To fight pathogen intrusion, the innate immune system uses a plethora of weapons, with the generation of oxidative and nitrosative stresses among the most efficient. In this work, the S. aureus genome-wide trans...

journal_title：Applied microbiology and biotechnology

authors： Nobre LS,Saraiva LM

更新日期：2013-03-01 00:00:00

abstract：:The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. I...

journal_title：Applied microbiology and biotechnology

authors： Kakeshita H,Kageyama Y,Ara K,Ozaki K,Nakamura K

更新日期：2011-03-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:Pseudomonas chlororaphis GP72 is a root-colonizing biocontrol strain isolated from the green pepper rhizosphere that synthesizes two phenazine derivatives: phenazine-1-carboxylic acid (PCA) and 2-hydroxyphenazine (2-OH-PHZ). The 2-OH-PHZ derivative shows somewhat stronger broad-spectrum antifungal activity than PCA, b...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Chen MM,Wang W,Hu HB,Peng HS,Xu YQ,Zhang XH

更新日期：2011-01-01 00:00:00

abstract：:Catalytic antibodies are currently being investigated in order to understand their role under physio-pathological situations. To this end, the knowledge of structure-function relationships is of great interest. Recombinant scFv fragments are smaller and easier to genetically manipulate than whole antibodies, making th...

journal_title：Applied microbiology and biotechnology

authors： Ben Naya R,Matti K,Guellier A,Matagne A,Boquet D,Thomas D,Friboulet A,Avalle B,Padiolleau-Lefèvre S

更新日期：2013-09-01 00:00:00

abstract：:κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Li G,Mo Z,Mou H

更新日期：2013-12-01 00:00:00

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:We used experimental evolution in order to identify genes involved in the adaptation of Saccharomyces cerevisiae to the early stages of alcoholic fermentation. Evolution experiments were run for about 200 generations, in continuous culture conditions emulating the initial stages of wine fermentation. We performed whol...

journal_title：Applied microbiology and biotechnology

authors： Mangado A,Tronchoni J,Morales P,Novo M,Quirós M,Gonzalez R

更新日期：2015-02-01 00:00:00

abstract：:Due to variations in the production levels, a full-scale sequencing batch reactor (SBR) for post-treatment of tannery wastewater was exposed to low and high ammonia load periods. In order to study how these changes affected the N-removal capacity, the microbiology of the reactor was studied by a diverse set of techniq...

journal_title：Applied microbiology and biotechnology

authors： Cabezas A,Draper P,Etchebehere C

更新日期：2009-10-01 00:00:00

abstract：:Geminiviruses have evolved with tremendous potential of recombination and possess the ability to manipulate several cellular processes of hosts. Chilli leaf curl virus (ChiLCV) is a monopartite Begomovirus (family Geminiviridae) which has emerged as a serious threat to chilli production worldwide. To date, development...

journal_title：Applied microbiology and biotechnology

authors： Kushwaha N,Sahu PP,Prasad M,Chakraborty S

更新日期：2015-06-01 00:00:00

abstract：:The morbidity and mortality associated with bacterial infections have remained significant despite chemotherapeutic advances. With the emergence of drug-resistant bacterial strains, the situation has become a serious threat to the public health. Thus, there is an urgent need to identify novel antibacterials. The major...

journal_title：Applied microbiology and biotechnology

authors： Akbar N,Siddiqui R,Sagathevan KA,Khan NA

更新日期：2019-05-01 00:00:00

abstract：:Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the...

journal_title：Applied microbiology and biotechnology

authors： Modig T,Granath K,Adler L,Lidén G

更新日期：2007-05-01 00:00:00

abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

journal_title：Applied microbiology and biotechnology

authors： Adav SS,Lee DJ

更新日期：2008-03-01 00:00:00

abstract：:Cell-surface functional groups (amino, carboxyl, hydroxyl, as well as phosphate) were chemically modified in various ways to enhance the demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1. Results demonstrated that the demulsifying activity was significantly inhibited by amino enrichment wi...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Liu J,Huang X,Lu L,Peng K

更新日期：2017-05-01 00:00:00

abstract：:We describe a novel chromosome engineering technique for shuffling selected regions of chromosomes from two strains in Saccharomyces cerevisiae: The technique starts with the construction of MATa and MATalpha strains in which a particular chromosome is split at exactly the same site in both strains such that the split...

journal_title：Applied microbiology and biotechnology

authors： Sugiyama M,Yamamoto E,Mukai Y,Kaneko Y,Nishizawa M,Harashima S

更新日期：2006-10-01 00:00:00

abstract：:Alcoholic fermentation of grape must is a complex process, involving several yeast genera and species. The early stages in fermentation are dominated by non-Saccharomyces yeasts that are gradually replaced by the Saccharomyces cerevisiae species, which takes over the fermentation. Quantitative studies have reported th...

journal_title：Applied microbiology and biotechnology

authors： Renault PE,Albertin W,Bely M

更新日期：2013-05-01 00:00:00

abstract：:In this work, fermentation and formulation aspects of the nematophagous fungus Hirsutella rhossiliensis BBA were investigated. When incubated in 2% (w/w) glucose and 0.5% (w/w) yeast extract medium in a 1-L Erlenmeyer flask without baffles, heavy pellet formation was observed. Only 40% of the mycelium had a size less ...

journal_title：Applied microbiology and biotechnology

authors： Patel AV,Jakobs-Schönwandt D,Rose T,Vorlop KD

更新日期：2011-03-01 00:00:00

abstract：:Even though salbutamol (SAL) had remarkable effects on the enhancement of growth rate and carcass composition in different livestock species such as cattle, pigs, sheep and poultry, it was banned as a growth promoter because of its adverse effects on health. However, the specific mechanism by which salbutamol enhances...

journal_title：Applied microbiology and biotechnology

authors： Lu C,Zhou J,Li Y,Zhang D,Wang Z,Li Y,Cheong L,Zhang C,Su X

更新日期：2017-07-01 00:00:00

abstract：:As an alternative class of antimicrobial agents used to overcome drug-resistant infections, antimicrobial peptides (AMPs) have recently gained significant attention. In this study, we designed an improved antimicrobial peptide, K1K8, based on the molecular template of Hp1404. Compared to the wild-type Hp1404, K1K8 sho...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Liu G,Meng L,Yu W,Xu X,Li W,Wu Y,Cao Z

更新日期：2016-06-01 00:00:00

abstract：:D-Tagatose is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and is rarely found in nature. Recently, there has been industrial interest in D-tagatose as a low-calorie sugar-substituting sweetener. This article describes the properties and metabolism of tagatose as well as its commercial impo...

journal_title：Applied microbiology and biotechnology

authors： Kim P

更新日期：2004-08-01 00:00:00

abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

journal_title：Applied microbiology and biotechnology

authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

更新日期：2010-08-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h(-1) but accumulated almost no poly-3-hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (mu = 0....

journal_title：Applied microbiology and biotechnology

authors： Sun Z,Ramsay JA,Guay M,Ramsay BA

更新日期：2007-02-01 00:00:00