Fermentation of protopanaxadiol type ginsenosides (PD) with probiotic Bifidobacterium lactis and Lactobacillus rhamnosus.

Abstract:

:Ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities. In this study, major protopanaxadiol type ginsenosides (PD) Rb1, Rc, and Rb2 were isolated from Panax ginseng and were transformed using two probiotic strains namely Bifidobacterium lactis Bi-07 and Lactobacillus rhamnosus HN001 to obtain specific deglycosylated ginsenosides. It was demonstrated that B. lactis transformed ginsenosides Rb1, Rc, and Rb2 to Rd within 1 h of fermentation and rare ginsenoside F2 by the conversion of Rd after 12-h fermentation. The maximum Rd concentration was 147.52 ± 1.45 μg/mL after 48-h fermentation as compared to 45.85 ± 0.71 μg/mL before fermentation. In contrast, L. rhamnosus transformed Rb1, Rc, and Rb2 into Rd as the final metabolite after 72-h fermentation. B. lactis displayed significantly (p < 0.05) higher β-glucosidase activity against p-nitrophenyl-β-glucopyranoside than L. rhamnosus and higher bioconversion efficiency during fermentation. The present study suggests that the fermentation of major PD type ginsenosides with B. lactis Bi-07 may serve as an effective means to afford bioactive deglycosylated ginsenosides and to create novel ginsenoside extracts.

authors

Tan JS,Yeo CR,Popovich DG

doi

10.1007/s00253-017-8295-4

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

5427-5437

issue

13

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-017-8295-4

journal_volume

101

pub_type

杂志文章
  • Application of urea-agarose gel electrophoresis to select non-redundant 16S rRNAs for taxonomic studies: palladium(II) removal bacteria.

    abstract::The 16S ribosomal RNA (rRNA) gene has been the most commonly used sequence to characterize bacterial communities. The classical approach to obtain gene sequences to study bacterial diversity implies cloning amplicons, selecting clones, and Sanger sequencing cloned fragments. A more recent approach is direct sequencing...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-7163-3

    authors: Assunção A,Costa MC,Carlier JD

    更新日期:2016-03-01 00:00:00

  • Auto-aggregation properties of a novel aerobic denitrifier Enterobacter sp. strain FL.

    abstract::Enterobacter sp. strain FL was newly isolated from activated sludge and exhibited significant capability of auto-aggregation as well as aerobic denitrification. The removal efficiencies of NO3--N, total nitrogen (TN), and TOC by strain FL in batch culture reached 94.6, 63.9, and 72.5% in 24 h, respectively. The produc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8720-8

    authors: Wang X,An Q,Zhao B,Guo JS,Huang YS,Tian M

    更新日期:2018-02-01 00:00:00

  • Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology.

    abstract::The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-2446-1

    authors: Clomburg JM,Gonzalez R

    更新日期:2010-03-01 00:00:00

  • Single cell oil production on molasses by Yarrowia lipolytica strains overexpressing DGA2 in multicopy.

    abstract::Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted fo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6733-8

    authors: Gajdoš P,Nicaud JM,Rossignol T,Čertík M

    更新日期:2015-10-01 00:00:00

  • Redirection of metabolism during nutrient feeding in fed-batch cultures of Bacillus thuringiensis.

    abstract::During sporulation, Bacillus thuringiensis produces insecticidal crystal inclusions (Cry proteins) encoded by cry genes. In fed-batch cultures (FBCs), spores and Cry protein yields are usually low, so we therefore studied the pattern of metabolic changes occurring in batch cultures and FBCs of a B. thuringiensis strai...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1724-1

    authors: López-y-López VE,de la Torre M

    更新日期:2005-04-01 00:00:00

  • Rapid adaptation of Rhodococcus erythropolis cells to salt stress by synthesizing polyunsaturated fatty acids.

    abstract::Bacterial cells are known to adapt to challenging environmental conditions such as osmotic stress. However, most of the work done in this field describes the adaptation of growing populations where the new generations acquire traits that improve their ability to survive. In the present study, the responses of Rhodococ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5549-2

    authors: de Carvalho CC,Marques MP,Hachicho N,Heipieper HJ

    更新日期:2014-06-01 00:00:00

  • Detection of human chorionic gonadotrophin hormone using a label-free epoxysilane-modified capacitive immunosensor.

    abstract::A new and label-free capacitive immunosensor based on antibody-functionalized epoxysilane on a glassy carbon electrode has been developed for quantitative detection of human chorionic gonadotropin (hCG). Monitoring the changes in the capacitance signals of antibodies before and after the binding of the antigen provide...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0778-7

    authors: Liao JY

    更新日期:2007-04-01 00:00:00

  • Bacteriophage-encoded virion-associated enzymes to overcome the carbohydrate barriers during the infection process.

    abstract::Bacteriophages are bacterial viruses that infect the host after successful receptor recognition and adsorption to the cell surface. The irreversible adherence followed by genome material ejection into host cell cytoplasm must be preceded by the passage of diverse carbohydrate barriers such as capsule polysaccharides (...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-017-8224-6

    authors: Latka A,Maciejewska B,Majkowska-Skrobek G,Briers Y,Drulis-Kawa Z

    更新日期:2017-04-01 00:00:00

  • Bivariate genome-wide association study of the growth plasticity of Staphylococcus aureus in coculture with Escherichia coli.

    abstract::Phenotypic plasticity is the capacity to change the phenotype in response to different environments without alteration of the genotype. Despite sufficient evidence that microorganisms have a major role in the fitness and sickness of eukaryotes, there has been little research regarding microbial phenotypic plasticity. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10636-6

    authors: Zheng X,Bai J,Ye M,Liu Y,Jin Y,He X

    更新日期:2020-06-01 00:00:00

  • Intraspecific diversity of Oenococcus oeni strains determined by sequence analysis of target genes.

    abstract::Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0487-2

    authors: Delaherche A,Bon E,Dupé A,Lucas M,Arveiler B,De Daruvar A,Lonvaud-Funel A

    更新日期:2006-11-01 00:00:00

  • Analysis of coenzyme A activated compounds in actinomycetes.

    abstract::Acyl-CoAs are crucial compounds involved in essential metabolic pathways such as the Krebs cycle and lipid, carbohydrate, and amino acid metabolisms, and they are also key signal molecules involved in the transcriptional regulation of lipid biosynthesis in many organisms. In this study, we took advantage of the high s...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7635-0

    authors: Cabruja M,Lyonnet BB,Millán G,Gramajo H,Gago G

    更新日期:2016-08-01 00:00:00

  • The pentose phosphate pathway leads to enhanced succinic acid flux in biofilms of wild-type Actinobacillus succinogenes.

    abstract::Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four d...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7763-6

    authors: Bradfield MF,Nicol W

    更新日期:2016-11-01 00:00:00

  • Perspectives and advances of biological H2 production in microorganisms.

    abstract::The rapid development of clean fuels for the future is a critically important global challenge for two main reasons. First, new fuels are needed to supplement and ultimately replace depleting oil reserves. Second, fuels capable of zero CO2 emissions are needed to slow the impact of global warming. This review summariz...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-006-0528-x

    authors: Rupprecht J,Hankamer B,Mussgnug JH,Ananyev G,Dismukes C,Kruse O

    更新日期:2006-09-01 00:00:00

  • Production and characterization of medium-chain-length polyhydroxyalkanoate with high 3-hydroxytetradecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442.

    abstract::Medium-chain-length polyhydroxyalkanoates (mcl-PHA) consisting of 3-hydroxyhexanoate (HHx), 3-hydroxyoctanoate (HO), 3-hydroxydecanoate, 3-hydroxydodecanoate, and high-content 3-hydroxytetradecanoate (HTD) was produced by knockout mutant Pseudomonas putida KT2442 termed P. putida KTOY06. When grown on 6 to14 g/L singl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1092-8

    authors: Liu W,Chen GQ

    更新日期:2007-10-01 00:00:00

  • β-Glucuronidase from Lactobacillus brevis useful for baicalin hydrolysis belongs to glycoside hydrolase family 30.

    abstract::Baicalin (baicalein 7-O-β-D-glucuronide) is one of the major flavonoid glucuronides found in traditional herbal medicines. Because its aglycone, baicalein, is absorbed more quickly and shows more effective properties than baicalin, the conversion of baicalin into baicalein by β-glucuronidase (GUS) has drawn the attent...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5325-8

    authors: Sakurama H,Kishino S,Uchibori Y,Yonejima Y,Ashida H,Kita K,Takahashi S,Ogawa J

    更新日期:2014-05-01 00:00:00

  • Genetically engineered fusion of allergen and viral-like particle induces a more effective allergen-specific immune response than a combination of them.

    abstract::Chimeric virus-like particles (VLPs) were developed as a candidate for allergen-specific immunotherapy. In this study, hepatitis B core antigen (HBcAg) that genetically fused to Chenopodium album polcalcin (Che a 3)-derived peptide was expressed in E. coli BL21, purified, and VLP formation was evaluated using native a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-11012-0

    authors: Sani MZ,Bargahi A,Momenzadeh N,Dehghani P,Moghadam MV,Maleki SJ,Nabipour I,Shirkani A,Akhtari J,Hesamizadeh K,Heidari S,Omrani F,Akbarzadeh S,Mohammadi M

    更新日期:2021-01-01 00:00:00

  • Heterologous protein secretion in Lactococcus lactis is enhanced by the Bacillus subtilis chaperone-like protein PrsA.

    abstract::The Bacillus subtilis lipoprotein PrsA enhances the yield of several homologous and heterologous exported proteins in B. subtilis by being involved in the posttranslocational stage of the secretion process. In this work, we have studied the effect of B. subtilis PrsA on the secretion of Bacillus amyloliquefaciens alph...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0551-y

    authors: Lindholm A,Ellmén U,Tolonen-Martikainen M,Palva A

    更新日期:2006-12-01 00:00:00

  • Protein expression analysis revealed a fine-tuned mechanism of in situ detoxification pathway for the tolerant industrial yeast Saccharomyces cerevisiae.

    abstract::Inhibitory compounds liberated from lignocellulose pretreatment are representative toxic chemicals that repress microbial growth and metabolism. A tolerant strain of the industrial yeast Saccharomyces cerevisiae is able to detoxify a major class of toxic compounds while producing ethanol. Knowledge on the yeast tolera...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09906-9

    authors: Liu ZL,Huang X,Zhou Q,Xu J

    更新日期:2019-07-01 00:00:00

  • Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis.

    abstract::Polyketides, such as erythromycin, are complex natural products with diverse therapeutic applications. They are synthesized by multi-modular megaenzymes, so-called polyketide synthases (PKSs). The macrolide core of erythromycin, 6-deoxyerythronolide B (6dEB), is produced by the deoxyerythronolide B synthase (DEBS) tha...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6990-6

    authors: Kumpfmüller J,Methling K,Fang L,Pfeifer BA,Lalk M,Schweder T

    更新日期:2016-02-01 00:00:00

  • Phosphate starvation controls lactose metabolism to produce recombinant protein in Escherichia coli.

    abstract::Phosphate is one of the major constituents in growth media. It closely regulates central carbon and energy metabolism. Biochemical reactions in central carbon metabolism are known to be regulated by phosphorylation and dephosphorylation of enzymes. Phosphate scarcity can limit microbial productivity. However, microorg...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10935-y

    authors: Pandi K,Chauhan AS,Khan WH,Rathore AS

    更新日期:2020-11-01 00:00:00

  • An antifungal peptide from baby lima bean.

    abstract::A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0504-5

    authors: Wang HX,Ng TB

    更新日期:2006-12-01 00:00:00

  • Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost.

    abstract::Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compou...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8211-y

    authors: Ravi K,García-Hidalgo J,Gorwa-Grauslund MF,Lidén G

    更新日期:2017-06-01 00:00:00

  • Cellulose production and cellulose synthase gene detection in acetic acid bacteria.

    abstract::The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6198-1

    authors: Valera MJ,Torija MJ,Mas A,Mateo E

    更新日期:2015-02-01 00:00:00

  • Effects of addition of elicitors on rimocidin biosynthesis in Streptomyces rimosus M527.

    abstract::The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, is highly effective against a broad range of fungal plant pathogens, but at low yields. Elicitation is an effective method of stimulating the yield of bioactive secondary metabolites. In this study, the biomass and filtrate of a culture broth of E...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10565-4

    authors: Song Z,Ma Z,Bechthold A,Yu X

    更新日期:2020-05-01 00:00:00

  • Understanding the mechanistic roles of 30Kc6 gene in apoptosis and specific productivity in antibody-producing Chinese hamster ovary cells.

    abstract::Previously, we reported that the expression of Bombyx mori 30Kc6 gene in Chinese hamster ovary (CHO) cells increases recombinant protein production by both inhibiting apoptosis and enhancing specific productivity. In this study, in order to gain a thorough understanding of the roles of 30Kc6 gene in antibody productio...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-3899-1

    authors: Wang Z,Ma X,Fan L,Rhee WJ,Park TH,Zhao L,Tan WS

    更新日期:2012-06-01 00:00:00

  • Mannitol production by heterofermentative Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in free and controlled pH batch fermentations.

    abstract::Certain lactic acid bacteria, especially heterofermentative strains, are capable to produce mannitol under adequate culture conditions. In this study, mannitol production by Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in modified MRS medium containing a mixture of fructose and glucose in a 6.5:1...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3617-4

    authors: Rodríguez C,Rimaux T,Fornaguera MJ,Vrancken G,de Valdez GF,De Vuyst L,Mozzi F

    更新日期:2012-03-01 00:00:00

  • The effect of acetylated xylan and sugar beet pulp on the expression and secretion of enzymes by Penicillium purpurogenum.

    abstract::Sugar beet pulp is a natural carbon source composed mainly of pectin and cellulose, which is utilized and degraded by the ascomycete Penicillium purpurogenum. The fungus also grows on and degrades acetylated xylan which lacks cellulose and pectin. Both carbon sources have been used in our laboratory to grow the fungus...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3744-y

    authors: Navarrete M,Callegari E,Eyzaguirre J

    更新日期:2012-01-01 00:00:00

  • Nitrification and degradation of halogenated hydrocarbons--a tenuous balance for ammonia-oxidizing bacteria.

    abstract::The process of nitrification has the potential for the in situ bioremediation of halogenated compounds provided a number of challenges can be overcome. In nitrification, the microbial process where ammonia is oxidized to nitrate, ammonia-oxidizing bacteria (AOB) are key players and are capable of carrying out the biod...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-010-2454-1

    authors: Sayavedra-Soto LA,Gvakharia B,Bottomley PJ,Arp DJ,Dolan ME

    更新日期:2010-03-01 00:00:00

  • Synthesis of biosurfactants in extreme conditions.

    abstract::The interest in industrial biotechnology and its importance opens up challenging possibilities of research in this area. Surfactants have long been among the most versatile of process chemicals. Their market is extremely competitive and manufacturers will have to expand their arsenal to develop products for the year 2...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s002530051329

    authors: Cameotra SS,Makkar RS

    更新日期:1998-11-01 00:00:00

  • Treatment of cyanide-containing wastewater from the food industry in a laboratory-scale fixed-bed methanogenic reactor.

    abstract::During the process of producing cassava starch from Manihot esculenta roots, large amounts of cyanoglycosides were released, which rapidly decayed to CN- following enzymatic hydrolysis. Depending on the varying cyanoglycoside content of the cassava varieties, the cyanide concentration in the wastewater was as high as ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051161

    authors: Siller H,Winter J

    更新日期:1998-02-01 00:00:00