Introduction of heterologous transcription factors and their target genes into Penicillium oxalicum leads to increased lignocellulolytic enzyme production.

abstract：:We used biodegradable plastics as fermentation substrates for the filamentous fungus Aspergillus oryzae. This fungus could grow under culture conditions that contained emulsified poly-(butylene succinate) (PBS) and emulsified poly-(butylene succinate-co-adipate) (PBSA) as the sole carbon source, and could digest PBS a...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Yamagata Y,Abe K,Hasegawa F,Machida M,Ishioka R,Gomi K,Nakajima T

更新日期：2005-06-01 00:00:00

abstract：:Extremophilic microorganisms are adapted to survive in ecological niches such as at high temperatures, extremes of pH, high salt concentrations and high pressure. These microorganisms produce unique biocatalysts that function under extreme conditions comparable to those prevailing in various industrial processes. Some...

journal_title：Applied microbiology and biotechnology

authors： Niehaus F,Bertoldo C,Kähler M,Antranikian G

更新日期：1999-06-01 00:00:00

abstract：:Regulatable promoters are important genetic tools, particularly for assigning function to essential and redundant genes. They can also be used to control the expression of enzymes that influence metabolic flux or protein secretion, thereby optimizing product yield in bioindustry. This review will focus on regulatable ...

journal_title：Applied microbiology and biotechnology

authors： Fuller KK,Dunlap JC,Loros JJ

更新日期：2018-05-01 00:00:00

abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

journal_title：Applied microbiology and biotechnology

authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

更新日期：2014-08-01 00:00:00

abstract：:Nanobiotechnology raises fascinating possibilities for new analytical assays in various fields such as bioelectronic assembly, biomechanics and sampling techniques, as well as in chips or micromachined devices. Recently, nanotechnology has greatly impacted biotechnological research with its potential applications in s...

journal_title：Applied microbiology and biotechnology

authors： Lee SJ,Lee SY

更新日期：2004-04-01 00:00:00

abstract：:Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates. Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function, which...

journal_title：Applied microbiology and biotechnology

authors： Tiemeyer A,Link H,Weuster-Botz D

更新日期：2007-08-01 00:00:00

abstract：:Targeted gene mutation by allelic replacement is important for functional genomic analysis and metabolic engineering. However, it is challenging in mutating the essential genes with the traditional method by using a selection marker, since the first step of essential gene knockout will result in a lethal phenotype. He...

journal_title：Applied microbiology and biotechnology

authors： Yang S,Cao X,Yu W,Li S,Zhou YJ

更新日期：2020-04-01 00:00:00

abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

journal_title：Applied microbiology and biotechnology

authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

更新日期：2002-10-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00

abstract：:Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett-Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these wer...

journal_title：Applied microbiology and biotechnology

authors： Minocha N,Kaur P,Satyanarayana T,Kunze G

更新日期：2007-08-01 00:00:00

abstract：:The behavior of Streptomyces peucetius var. caesius N47 was studied in a glucose limited chemostat with a complex cultivation medium. The steady-state study yielded the characteristic constants mu (max) over 0.10 h(-1), Y (XS) 0.536 g g(-1), and m(S) 0.54 mg g(-1) h(-1). The product of secondary metabolism, epsilon-rh...

journal_title：Applied microbiology and biotechnology

authors： Kiviharju K,Moilanen U,Leisola M,Eerikäinen T

更新日期：2007-01-01 00:00:00

abstract：:A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some...

journal_title：Applied microbiology and biotechnology

authors： Zhu M,Hu X,Cao G,Xue R,Gong C

更新日期：2018-08-01 00:00:00

abstract：:The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

journal_title：Applied microbiology and biotechnology

authors： Valera MJ,Torija MJ,Mas A,Mateo E

更新日期：2015-02-01 00:00:00

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:Monascus purpureus, a pigment-producing ascomycetous fungus, has been traditionally used for red rice preparation using solid-state fermentation. The objective of this study was to develop an improved pigment-producing strain of M. purpureus MTCC 1090 through genome shuffling followed by detailed analytical estimation...

journal_title：Applied microbiology and biotechnology

authors： Ghosh S,Dam B

更新日期：2020-12-01 00:00:00

abstract：:The L-rhamnose isomerase gene (L-rhi) encoding for L-rhamnose isomerase (L-RhI) from Bacillus pallidus Y25, a facultative thermophilic bacterium, was cloned and overexpressed in Escherichia coli with a cooperation of the 6xHis sequence at a C-terminal of the protein. The open reading frame of L-rhi consisted of 1,236 ...

journal_title：Applied microbiology and biotechnology

authors： Poonperm W,Takata G,Okada H,Morimoto K,Granström TB,Izumori K

更新日期：2007-10-01 00:00:00

abstract：:The oxidizing activity of CYP109B1 from Bacillus subtilis was reconstituted in vitro with various artificial redox proteins including putidaredoxin reductase and putidaredoxin from Pseudomonas putida, truncated bovine adrenodoxin reductase and adrenodoxin, flavodoxin reductase and flavodoxin from Escherichia coli, and...

journal_title：Applied microbiology and biotechnology

authors： Girhard M,Klaus T,Khatri Y,Bernhardt R,Urlacher VB

更新日期：2010-06-01 00:00:00

abstract：:Extremely acidophilic microorganisms have an optimal pH of <3 and are found in all three domains of life. As metals are more soluble at acid pH, acidophiles are often challenged by very high metal concentrations. Acidophiles are metal-tolerant by both intrinsic, passive mechanisms as well as active systems. Passive me...

journal_title：Applied microbiology and biotechnology

authors： Dopson M,Holmes DS

更新日期：2014-10-01 00:00:00

abstract：:Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

journal_title：Applied microbiology and biotechnology

authors： McIntyre DA,Harlander SK

更新日期：1993-11-01 00:00:00

abstract：:Catechin is a plant polyphenol composed of epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG) as diastereoisomers. Among the various classes of flavonoids, catechin was found to be the most powerful free radical scavenger, scavenging the reactive oxygen species (RO...

journal_title：Applied microbiology and biotechnology

authors： Fathima A,Rao JR

更新日期：2016-07-01 00:00:00

abstract：:In this study, an evolved Saccharomyces cerevisiae HJ7-14 with high ability of algae-based ethanol production was characterized by multi-omic approaches. Genome sequencing of the HJ7-14 revealed a point mutation in the GAL83 gene (G703A) involved in the catabolite repression as well as the galactose metabolism. Cultur...

journal_title：Applied microbiology and biotechnology

authors： Kim SJ,Lee JE,Lee DY,Park H,Kim KH,Park YC

更新日期：2018-10-01 00:00:00

abstract：:Forty-six pulp-bleaching fungi were screened for production of key enzymes for conversion of polychlorinated dibenzo-p-dioxins--lignin peroxidase (LiP), manganese peroxidase (MnP), and manganese-independent peroxidase (MiP)--under various conditions that would allow their utilization in the environment. Of 38 MnP-prod...

journal_title：Applied microbiology and biotechnology

authors： Manji S,Ishihara A

更新日期：2004-01-01 00:00:00

abstract：:The hemiascomycete Ashbya gossypii develops a mycelium. Nutritional stress leads to its differentiation into sporangia. These generate spores. In parallel, the yellow pigment riboflavin is produced. Intracellularly accumulated riboflavin, made visible as a bright green fluorescence, was observed in only 60% of the hyp...

journal_title：Applied microbiology and biotechnology

authors： Nieland S,Stahmann KP

更新日期：2013-12-01 00:00:00

abstract：:Globotriose (Galα1-4Galβ1-4Glc) is an important cell surface epitope that acts as the receptor for Shiga-like toxins, and it is also the core structure of Globo H and SSEA4 that are tumor-associated glycans. Hence, the enzymatic synthesis of globotriose would be necessary for the development of carbohydrate-based ther...

journal_title：Applied microbiology and biotechnology

authors： Gong W,Xu L,Gu G,Lu L,Xiao M

更新日期：2016-08-01 00:00:00

abstract：:Potentiometric biosensors for the determination of organophosphorus (OP) insecticides were developed by applying either immobilized whole cells or cytoplasmic membrane fractions of wild-type Flavobacterium sp. on the surface of a glass pH electrode. The ability of Flavobacterium sp. to degrade OP compounds as sole car...

journal_title：Applied microbiology and biotechnology

authors： Gäberlein S,Spener F,Zaborosch C

更新日期：2000-11-01 00:00:00

abstract：:Malate synthase (Mls), a key enzyme in the glyoxylate cycle, is required for virulence in microbial pathogens. In this study, we identified the AoMls gene from the nematode-trapping fungus Arthobotrys oligospora. The gene contains 4 introns and encodes a polypeptide of 540 amino acids. To characterize the function of ...

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Wang Y,Zhao Y,Huang Y,Zhang KQ,Yang J

更新日期：2014-03-01 00:00:00

abstract：:The engineered Saccharomyces cerevisiae strain △faa1△faa4 [Acot5s] was demonstrated to accumulate more free fatty acids (FFA) previously. Here, comparative proteomic analysis was performed to get a global overview of metabolic regulation in the strain. Over 500 proteins were identified, and 82 of those proteins were f...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lee JJL,Zhang J,Chen WN

更新日期：2016-02-01 00:00:00

abstract：:A reactive iron barrier in a contaminated aquifer with low pH was found to dechlorinate 1,2-dichloroethane (1,2-DCA) in situ. This chlorinated ethane is known to resist abiotic reduction by zero valent iron. Samples taken up-gradient and within the barrier were used to inoculate anaerobic batch cultures amended with v...

journal_title：Applied microbiology and biotechnology

authors： Zemb O,Lee M,Low A,Manefield M

更新日期：2010-09-01 00:00:00

abstract：:Catharanthus roseus (L.) G. Don is a plant species known for its production of a variety of terpenoid indole alkaloids, many of which have pharmacological activities. Production of catharanthine in cell cultures or in hairy roots established by transformation with Agrobacterium rhizogenes is of interest because cathar...

journal_title：Applied microbiology and biotechnology

authors： Zhou ML,Hou HL,Zhu XM,Shao JR,Wu YM,Tang YX

更新日期：2011-08-01 00:00:00