Acid phosphatase production by recombinant Arxula adeninivorans.

Abstract:

:Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett-Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these were optimized using response surface methodology of central composite design. The highest enzyme yields were attained in the medium with 3.9% sucrose and 1.6% peptone at pH 3.8. Because of optimization, 3.86- and 4.19-fold enhancement in enzyme production was achieved in shake flasks (17,054 U g(-1) DYB) and laboratory fermenter (18,465 U g(-1) DYB), respectively.

authors

Minocha N,Kaur P,Satyanarayana T,Kunze G

doi

10.1007/s00253-007-1021-x

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

387-93

issue

2

eissn

0175-7598

issn

1432-0614

journal_volume

76

pub_type

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