Tailor-made mannosylerythritol lipids: current state and perspectives.

abstract：:One of the factors affecting chronological life span (CLS) in budding yeast is nutrient, especially carbon limitation. Aside from metabolites in the growth medium such as glucose, amino acids, and acetic acid, many pharmaceuticals have also been proven to alter CLS. Besides their impact on life span, these drugs are a...

journal_title：Applied microbiology and biotechnology

authors： Borklu-Yucel E,Eraslan S,Ulgen KO

更新日期：2015-08-01 00:00:00

abstract：:Inhibitory compounds liberated from lignocellulose pretreatment are representative toxic chemicals that repress microbial growth and metabolism. A tolerant strain of the industrial yeast Saccharomyces cerevisiae is able to detoxify a major class of toxic compounds while producing ethanol. Knowledge on the yeast tolera...

journal_title：Applied microbiology and biotechnology

authors： Liu ZL,Huang X,Zhou Q,Xu J

更新日期：2019-07-01 00:00:00

abstract：:This study aimed to evaluate the effects of partial reducing rumen-protected Lys (RPLys) on rumen fermentation and microbial composition in heifers. Three ruminal fistulated Holstein Friesian bulls were used to determine the effective degradability of RPLys using an in situ method at incubation times of 0, 2, 6, 12, 1...

journal_title：Applied microbiology and biotechnology

authors： Kong F,Gao Y,Tang M,Fu T,Diao Q,Bi Y,Tu Y

更新日期：2020-08-01 00:00:00

abstract：:Betalamic acid is the structural unit of all the natural pigments betalains. These are nitrogen-containing water-soluble compounds with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales. The formation of betalamic acid from the precursor amino acid 3,4-dihydroxy-L-phenylalani...

journal_title：Applied microbiology and biotechnology

authors： Gandía-Herrero F,García-Carmona F

更新日期：2014-02-01 00:00:00

abstract：:Tacrolimus (FK506), an effective immunosuppressant, is widely used in the treatment of autoimmune diseases. In this study, we identified that BulZ, a Streptomyces antibiotic regulatory protein (SARP) family regulator, acted as a positive regulator for spore differentiation and tacrolimus production. A knockout of bulZ...

journal_title：Applied microbiology and biotechnology

authors： Ma D,Wang C,Chen H,Wen J

更新日期：2018-06-01 00:00:00

abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

journal_title：Applied microbiology and biotechnology

authors： Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

更新日期：1991-03-01 00:00:00

abstract：:For the fermentative production of plant-specific flavanones (naringenin, pinocembrin) by Escherichia coli, a plasmid was constructed which carried an artificial biosynthetic gene cluster, including PAL encoding a phenylalanine ammonia-lyase from a yeast, ScCCL encoding a cinnamate/coumarate:CoA ligase from the actino...

journal_title：Applied microbiology and biotechnology

authors： Miyahisa I,Kaneko M,Funa N,Kawasaki H,Kojima H,Ohnishi Y,Horinouchi S

更新日期：2005-09-01 00:00:00

abstract：:Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Williamson G

更新日期：1995-11-01 00:00:00

abstract：:In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon-contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic...

journal_title：Applied microbiology and biotechnology

authors： Benedek T,Szentgyörgyi F,Szabó I,Farkas M,Duran R,Kriszt B,Táncsics A

更新日期：2020-07-01 00:00:00

abstract：:Ethanol fermentation ability of the thermotolerant yeast Kluyveromyces marxianus, which is able to utilize various sugars including glucose, mannose, galactose, xylose, and arabinose, was examined under shaking and static conditions at high temperatures. The yeast was found to produce ethanol from all of these sugars ...

journal_title：Applied microbiology and biotechnology

authors： Rodrussamee N,Lertwattanasakul N,Hirata K,Suprayogi,Limtong S,Kosaka T,Yamada M

更新日期：2011-05-01 00:00:00

abstract：:The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

journal_title：Applied microbiology and biotechnology

authors： Fourage L,Colas B

更新日期：2001-08-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathwa...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Cai M,Zhou X,Li Z,Zhang Y

更新日期：2016-09-01 00:00:00

abstract：:During sporulation, Bacillus thuringiensis produces insecticidal crystal inclusions (Cry proteins) encoded by cry genes. In fed-batch cultures (FBCs), spores and Cry protein yields are usually low, so we therefore studied the pattern of metabolic changes occurring in batch cultures and FBCs of a B. thuringiensis strai...

journal_title：Applied microbiology and biotechnology

authors： López-y-López VE,de la Torre M

更新日期：2005-04-01 00:00:00

abstract：:A cytochrome P450 and an iron-sulfur protein, whose expression was specifically induced during growth of Mycobacterium sp. strain HE5 on morpholine as the sole source of carbon, nitrogen, and energy were purified to apparent homogeneity. Due to the lack of enzymatic activity, carbon monoxide difference spectra and det...

journal_title：Applied microbiology and biotechnology

authors： Sielaff B,Andreesen JR,Schräder T

更新日期：2001-08-01 00:00:00

abstract：:Crude cell extracts of Pseudomonas putida F6 transformed 4-substituted fluoro-, chloro-, bromo- and iodo-phenol without the exogenous addition of cofactors. The rate of substrate consumption decreased with increasing substituent size (F>Cl>Br>I). Biotransformations resulted in greater than 95% utilisation of the halog...

journal_title：Applied microbiology and biotechnology

authors： Brooks SJ,Doyle EM,Hewage C,Malthouse JP,Duetz W,O' Connor KE

更新日期：2004-05-01 00:00:00

abstract：:After crude protein of the marine yeast strains maintained in this laboratory was estimated by the method of Kjehldahl, we found that the G7a strain which was identified to be a strain of Cryptococcus aureus according to the routine identification and molecular methods contained high level of protein and could grow on...

journal_title：Applied microbiology and biotechnology

authors： Gao L,Chi Z,Sheng J,Ni X,Wang L

更新日期：2007-12-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, is the main pathogenic agent of the rapidly spreading pneumonia called coronavirus disease 2019 (COVID-19). SARS-CoV-2 infects much more people, especially the elder population, around the world than other coronavirus, such as SAR...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Liao B,Cheng L,Peng X,Xu X,Li Y,Hu T,Li J,Zhou X,Ren B

更新日期：2020-09-01 00:00:00

abstract：:Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. To achieve high efficient biotransformation of benzene to benzene cis-diols, Pseudomonas putida KT2442, Pseudomonas stutzeri 1317, and Aeromonas hydrophila 4AK4 were used as hosts to express TDO gene tod. Plasmid pSPM01, a deriva...

journal_title：Applied microbiology and biotechnology

authors： Ouyang SP,Sun SY,Liu Q,Chen J,Chen GQ

更新日期：2007-02-01 00:00:00

abstract：:Metarhizium robertsii is a fungus with two lifestyles; it is a plant root symbiont and an insect pathogen. A spontaneously phenotypically degenerated strain of M. robertsii strain ARSEF 2575 (M. robertsii lc-2575; lc = low conidiation) showed a reduction in conidiation and fungal virulence after successive subculturin...

journal_title：Applied microbiology and biotechnology

authors： Hu S,Bidochka MJ

更新日期：2020-06-01 00:00:00

abstract：:Plants as well as pro- and eukaryotic microorganisms are able to synthesise lysine via de novo synthesis. While plants and bacteria, with some exceptions, rely on variations of the meso-diaminopimelate pathway for lysine biosynthesis, fungi exclusively use the α-aminoadipate pathway. Although bacteria and fungi are, i...

journal_title：Applied microbiology and biotechnology

authors： Fazius F,Zaehle C,Brock M

更新日期：2013-05-01 00:00:00

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:Optimization of yields and productivities in reductive whole-cell biotransformations is an important issue for the industrial application of such processes. In a recent study with Escherichia coli, we analyzed the reduction of the prochiral β-ketoester methyl acetoacetate by an R-specific alcohol dehydrogenase (ADH) t...

journal_title：Applied microbiology and biotechnology

authors： Siedler S,Bringer S,Blank LM,Bott M

更新日期：2012-02-01 00:00:00

abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...

journal_title：Applied microbiology and biotechnology

authors： Ehrenreich A

更新日期：2006-11-01 00:00:00

abstract：:Due to their high secretion capacity, Gram-positive bacteria from the genus Bacillus are important expression hosts for the high-yield production of enzymes in industrial biotechnology; however, to date, strains from only few Bacillus species are used for enzyme production at industrial scale. Herein, we introduce Pae...

journal_title：Applied microbiology and biotechnology

authors： Heinze S,Zimmermann K,Ludwig C,Heinzlmeir S,Schwarz WH,Zverlov VV,Liebl W,Kornberger P

更新日期：2018-12-01 00:00:00

abstract：:Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

journal_title：Applied microbiology and biotechnology

authors： He X,Zhang Q,Cooney MJ,Yan T

更新日期：2015-07-01 00:00:00

abstract：:Despite the known hazardous effects of antimony (Sb) on human health, Sb monitoring biosensors have not been as actively investigated as arsenic (As) biosensors. Whole-cell bioreporters (WCBs) employing an arsenic-responsive operon and a regulatory protein (ArsR) are reportedly capable of monitoring arsenite, arsenate...

journal_title：Applied microbiology and biotechnology

authors： Lee W,Kim H,Jang G,Kim BG,Yoon Y

更新日期：2020-03-01 00:00:00

abstract：:The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

journal_title：Applied microbiology and biotechnology

authors： Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

更新日期：2006-10-01 00:00:00

abstract：:DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identit...

journal_title：Applied microbiology and biotechnology

authors： Sandalli C,Singh K,Modak MJ,Ketkar A,Canakci S,Demir I,Belduz AO

更新日期：2009-08-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) employing nucleic acid mimics as probes is becoming an emerging molecular tool in the microbiology area for the detection and visualization of microorganisms. However, the impact that locked nucleic acid (LNA) and 2'-O-methyl (2'-OMe) RNA modifications have on the probe that i...

journal_title：Applied microbiology and biotechnology

authors： Fontenete S,Carvalho D,Guimarães N,Madureira P,Figueiredo C,Wengel J,Azevedo NF

更新日期：2016-07-01 00:00:00