Analysis of acyl CoA ester intermediates of the mevalonate pathway in Saccharomyces cerevisiae.


:The mevalonate pathway plays an important role in providing the cell with a number of essential precursors for the synthesis of biomass constituents. With respect to their chemical structure, the metabolites of this pathway can be divided into two groups: acyl esters [acetoacetyl CoA, acetyl CoA, hydroxymethylglutaryl (HMG) CoA] and phosphorylated metabolites (isopentenyl pyrophosphate, dimethylallyl pyrophosphate, geranyl pyrophosphate, farnesyl pyrophosphate). In this study, we developed a method for the precise analysis of the intracellular concentration of acetoacetyl CoA, acetyl CoA and HMG CoA; and we used this method for quantification of these metabolites in Saccharomyces cerevisiae, both during batch growth on glucose and on galactose and in glucose-limited chemostat cultures operated at three different dilution rates. The level of the metabolites changed depending on the growth phase/specific growth rate and the carbon source, in a way which indicated that the synthesis of acetoacetyl CoA and HMG CoA is subject to glucose repression. In the glucose batch, acetyl CoA accumulated during the growth on glucose and, just after glucose depletion, HMG CoA and acetoacetyl CoA started to accumulate during the growth on ethanol. In the galactose batch, HMG CoA accumulated during the growth on galactose and a high level was maintained into the ethanol growth phase; and the levels of acetyl CoA and HMG CoA were more than two-fold higher in the galactose batch than in the glucose batch.


Seker T,Møller K,Nielsen J




Has Abstract


2005-04-01 00:00:00












  • Screening of cry-type promoters with strong activity and application in Cry protein encapsulation in a sigK mutant.

    abstract::To optimize the expression of cry genes in a Bacillus thuringiensis sigK mutant failing in crystal releasing, the transcriptional activity of the cry promoters cry1A, cry3A, cry4A, and cry8E was compared using lacZ gene fusions. A beta-galactosidase assay indicated that the cry8E promoter showed the highest transcript...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhou C,Zheng Q,Peng Q,Du L,Shu C,Zhang J,Song F

    更新日期:2014-09-01 00:00:00

  • Correlation of nitric oxide produced by an inducible nitric oxide synthase-like protein with enhanced expression of the phenylpropanoid pathway in Inonotus obliquus cocultured with Phellinus morii.

    abstract::Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fung...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhao Y,Xi Q,Xu Q,He M,Ding J,Dai Y,Keller NP,Zheng W

    更新日期:2015-05-01 00:00:00

  • High level expression and purification of antimicrobial human cathelicidin LL-37 in Escherichia coli.

    abstract::The human antimicrobial peptide LL-37 is a cationic peptide with antimicrobial activity against both Gram-positive and Gram-negative microorganisms. This work describes the development of an expression system based on Escherichia coli capable of high production of the recombinant LL-37. The fusion protein Trx-LL-37 wa...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Krahulec J,Hyrsová M,Pepeliaev S,Jílková J,Cerný Z,Machálková J

    更新日期:2010-09-01 00:00:00

  • Biosynthesis of bioactive O-methylated flavonoids in Escherichia coli.

    abstract::Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kim MJ,Kim BG,Ahn JH

    更新日期:2013-08-01 00:00:00

  • Micro total analysis system (micro-TAS) in biotechnology.

    abstract::Nanobiotechnology raises fascinating possibilities for new analytical assays in various fields such as bioelectronic assembly, biomechanics and sampling techniques, as well as in chips or micromachined devices. Recently, nanotechnology has greatly impacted biotechnological research with its potential applications in s...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Lee SJ,Lee SY

    更新日期:2004-04-01 00:00:00

  • Changing glycine 21 for glutamic acid in the beta-subunit of penicillin G acylase from Kluyvera citrophila prevents protein maturation.

    abstract::Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Prieto I,Rodríguez MC,Márquez G,Pérez-Aranda A,Barbero JL

    更新日期:1992-02-01 00:00:00

  • Continuous production of poly([R]-3-hydroxybutyrate) by Cupriavidus necator in a multistage bioreactor cascade.

    abstract::Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes featur...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Atlić A,Koller M,Scherzer D,Kutschera C,Grillo-Fernandes E,Horvat P,Chiellini E,Braunegg G

    更新日期:2011-07-01 00:00:00

  • Growth of Escherichia coli MG1655 on LB medium: monitoring utilization of amino acids, peptides, and nucleotides with transcriptional microarrays.

    abstract::Analysis of gene expression data related to assimilation and biosynthesis of nitrogen-containing compounds amino acids, peptides, and nucleotides was used to monitor availability of these nutrients to Escherichia coli MG1655 growing on Luria-Bertani medium. The data indicate that free amino acids and nucleotides only ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Baev MV,Baev D,Radek AJ,Campbell JW

    更新日期:2006-07-01 00:00:00

  • PAH utilization by Pseudomonas rhodesiae KK1 isolated from a former manufactured-gas plant site.

    abstract::Pseudomonas rhodesiae KK1 was isolated from a former manufactured-gas plant site, due to its ability to grow rapidly in a mixture of polycyclic aromatic hydrocarbons (PAHs). Radiorespirometric analysis revealed that strain KK1 was found to be able to mineralize anthracene, naphthalene and phenanthrene. Notably, phenan...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kahng HY,Nam K,Kukor JJ,Yoon BJ,Lee DH,Oh DC,Kam SK,Oh KH

    更新日期:2002-12-01 00:00:00

  • Performance of an acoustic settler versus a hollow fiber-based ATF technology for influenza virus production in perfusion.

    abstract::Process intensification and integration is crucial regarding an ever increasing pressure on manufacturing costs and capacities in biologics manufacturing. For virus production in perfusion mode, membrane-based alternating tangential flow filtration (ATF) and acoustic settler are the commonly described cell retention t...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Gränicher G,Coronel J,Trampler F,Jordan I,Genzel Y,Reichl U

    更新日期:2020-06-01 00:00:00

  • Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae.

    abstract::The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

    更新日期:2018-01-01 00:00:00

  • Clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate.

    abstract::Intervention strategies for obesity are global issues that require immediate attention. The objective of this study was to assess the possibility that Clostridium butyricum and its potential components could reduce lipogenesis. Co-culture experiments of Caco-2 cells and 1 × 10(6), 1 × 10(7), and 1 × 10(8) CFU/ml of C....

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhao X,Guo Y,Liu H,Gao J,Nie W

    更新日期:2014-09-01 00:00:00

  • An Indian scenario on renewable and sustainable energy sources with emphasis on algae.

    abstract::India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Hemaiswarya S,Raja R,Carvalho IS,Ravikumar R,Zambare V,Barh D

    更新日期:2012-12-01 00:00:00

  • Low-serum medium development for human diploid fibroblast microcarrier cultures.

    abstract::A new medium supplement mixture, PPRF92, has been developed to enable the serial subculture of human diploid fibroblasts (MRC-5 cells) on microcarriers. Furthermore, the PPRF92 supplements enable cell growth at serum levels as low as 1%. Through an optimization programme, the PPRF92 supplements have evolved into a sim...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Forestell SP,Kalogerakis N,Behie LA

    更新日期:1992-11-01 00:00:00

  • The pentose phosphate pathway leads to enhanced succinic acid flux in biofilms of wild-type Actinobacillus succinogenes.

    abstract::Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four d...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Bradfield MF,Nicol W

    更新日期:2016-11-01 00:00:00

  • Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana.

    abstract::Genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as Beauveria bassiana. To date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Ying SH,Feng MG,Keyhani NO

    更新日期:2013-04-01 00:00:00

  • Engineering of pyranose 2-oxidase from Peniophora gigantea towards improved thermostability and catalytic efficiency.

    abstract::To improve the stability and catalytic efficiency of pyranose 2-oxidase (P2Ox) by molecular enzyme evolution, we cloned P2Ox cDNA by RACE-PCR from a cDNA library derived from the basidiomycete Peniophora gigantea. The P2Ox gene was expressed in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically ac...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Bastian S,Rekowski MJ,Witte K,Heckmann-Pohl DM,Giffhorn F

    更新日期:2005-06-01 00:00:00

  • Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes.

    abstract::For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Sindelar G,Wendisch VF

    更新日期:2007-09-01 00:00:00

  • Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor.

    abstract::Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

    更新日期:2013-10-01 00:00:00

  • Isolation and lipid degradation profile of Raoultella planticola strain 232-2 capable of efficiently catabolizing edible oils under acidic conditions.

    abstract::The lipids (fats and oils) degradation capabilities of soil microorganisms were investigated for possible application in treatment of lipids-contaminated wastewater. We isolated a strain of the bacterium Raoultella planticola strain 232-2 that is capable of efficiently catabolizing lipids under acidic conditions such ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Sugimori D,Watanabe M,Utsue T

    更新日期:2013-01-01 00:00:00

  • 16S rRNA gene-based characterization of bacteria potentially associated with phosphate and carbonate precipitation from a granular autotrophic nitrogen removal bioreactor.

    abstract::A bench-scale granular autotrophic nitrogen removal bioreactor (completely autotrophic nitrogen removal over nitrite (CANON) system) used for the treatment of synthetic wastewater was analyzed for the identification of microbiota with potential capacity for carbonate and phosphate biomineral formation. 16S ribosomal R...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Gonzalez-Martinez A,Rodriguez-Sanchez A,Rivadeneyra MA,Rivadeneyra A,Martin-Ramos D,Vahala R,Gonzalez-Lopez J

    更新日期:2017-01-01 00:00:00

  • Genome-centered omics insight into the competition and niche differentiation of Ca. Jettenia and Ca. Brocadia affiliated to anammox bacteria.

    abstract::Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhao Y,Feng Y,Chen L,Niu Z,Liu S

    更新日期:2019-10-01 00:00:00

  • Production of surfactant and detergent-stable, halophilic, and alkalitolerant alpha-amylase by a moderately halophilic Bacillus sp. Strain TSCVKK.

    abstract::A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase product...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kiran KK,Chandra TS

    更新日期:2008-01-01 00:00:00

  • Multiple large segment deletion method for Corynebacterium glutamicum.

    abstract::A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

    更新日期:2005-11-01 00:00:00

  • A novel self-cleavable tag Zbasic-∆I-CM and its application in the soluble expression of recombinant human interleukin-15 in Escherichia coli.

    abstract::Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

    更新日期:2017-02-01 00:00:00

  • Improving mycelial morphology and adherent growth as well as metabolism of Monascus yellow pigments using nitrate resources.

    abstract::Mycelial adhesion affects cell growth and the production of water-soluble extracellular yellow pigment (EYP) in submerged fermentation with Monascus ruber CGMCC 10910. Two nitrates, NaNO3 and KNO3, were used as nitrogen sources for mitigating mycelial adhesion and improving the production of EYP in this study. The res...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Yang SZ,Huang ZF,Liu HQ,Hu X,Wu ZQ

    更新日期:2020-11-01 00:00:00

  • Two novel cyclic hexapeptides from the genetically engineered Actinosynnema pretiosum.

    abstract::Two novel cyclic hexapeptides designated actinosynneptides A (1) and B (2), together with three tryptophan containing diketopiperazines, namely cyclo(L-Trp-L-Trp) (3), cyclo(L-Trp-N-MeL-Trp) (4), and cyclo(N-MeL-Trp-N-MeL-Trp) (5), were isolated from the culture of the genetically engineered strain HGF052::asm18 deriv...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Lu C,Xie F,Shan C,Shen Y

    更新日期:2017-03-01 00:00:00

  • Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field.

    abstract::Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Shen G,Zhang S,Liu X,Jiang Q,Ding W

    更新日期:2018-11-01 00:00:00

  • A mitogen-activated protein kinase PoxMK1 mediates regulation of the production of plant-biomass-degrading enzymes, vegetative growth, and pigment biosynthesis in Penicillium oxalicum.

    abstract::Mitogen-activated protein kinase (MAPK) cascades are broadly conserved and play essential roles in multiple cellular processes, including fungal development, pathogenicity, and secondary metabolism. Their function, however, also exhibits species and strain specificity. Penicillium oxalicum secretes plant-biomass-degra...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Ma B,Ning YN,Li CX,Tian D,Guo H,Pang XM,Luo XM,Zhao S,Feng JX

    更新日期:2021-01-01 00:00:00

  • Expression of two kinds of recombinant glutamate dehydrogenase from Aeropyrum pernix with different N-terminal sequence length in Escherichia coli.

    abstract::Two recombinant Aeropyrum pernix glutamate dehydrogenase (GDH) enzymes with different length N-termini were cloned and expressed in Escherichia coli: sGDH begins with the amino acid sequence of the extracted native enzyme (M-Q-P-T-D-P-L-E-E), whereas lGDH begins with the sequence of the predicted ORF (M-E-V-L-A-L-Q-P-...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Helianti I,Morita Y,Murakami Y,Yokoyama K,Tamiya E

    更新日期:2002-08-01 00:00:00