当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Synthetic dendrimeric peptide active against biofilm and persister cells of Pseudomonas aeruginosa.

Synthetic dendrimeric peptide active against biofilm and persister cells of Pseudomonas aeruginosa.

Abstract：

:Antimicrobial dendrimeric peptides (AMDP) are a relatively new class of agents displaying repetitive functional groups on a branched core. Previously, we have investigated the length requirement for antimicrobial activity of peptides consisting of repeated arginine (R) and tryptophan (W) side chains and found that even short linear RW repeats are active, providing a starting point for a de novo design of multivalent structures. In this study, we synthesized and tested a new synthetic dendrimer, 2D-24, for its antimicrobial activity against Pseudomonas aeruginosa, including the wild-type PAO1 and its mucoid mutant PDO300. This synthetic AMDP was found to kill planktonic cells of both PAO1 and PDO300 in a dose-dependent manner, with nearly complete killing of both strains observed when treated with 50 μM of this agent. In addition to planktonic cells, 2D-24 was also found to kill biofilm cells of both strains in a dose-dependent manner. For example, treatment with 30 μM 2D-24 led to 94.4 ± 1.4 and 93.9 ± 4.2 % killing of PAO1 and PDO300 biofilm cells, respectively. Furthermore, 2D-24 was effective in killing multidrug-tolerant persister cells of PAO1 and PDO300. While higher concentrations of 2D-24 were required to kill persister cells, combinations of 2D-24 with ciprofloxacin, tobramycin, or carbenicillin showed synergistic effects on killing persister cells of both strains. Based on hemolysis assays using sheep erythrocytes and a coculture model of PAO1 and human epithelial cells, 2D-24 was found to kill P. aeruginosa cells at concentrations that are not toxic to mammalian cells.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Bahar AA,Liu Z,Totsingan F,Buitrago C,Kallenbach N,Ren D

doi

10.1007/s00253-015-6645-7

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

8125-35

issue

19

eissn

0175-7598

issn

1432-0614

journal_volume

99

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli.

    abstract：:Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters, the one for alcohol dehydrogenase (PadhE) was found most effective. Recombinant E. coli JM 109 (pWCY09) ha...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1816-4

    authors： Wei XX,Shi ZY,Yuan MQ,Chen GQ

    更新日期：2009-03-01 00:00:00

  • Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis.

    abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-4728-x

    authors： Seo MH,Kim KR,Oh DK

    更新日期：2013-10-01 00:00:00

  • Inhibition of oxidative stress-induced amyloid beta formation in NT2 neurons by culture filtrate of a strain of Streptomyces antibioticus.

    abstract：:Actinomycetes isolated from Iran soil habitats were tested for the capacity to produce compounds which can protect neurons from cell death generated by oxidative stress in NT2 neurons. Confirmation of our initial hit was accomplished via the determination of amyloid beta level using the enzyme-linked immunosorbent ass...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2456-z

    authors： Eftekharzadeh B,Hamedi J,Mohammadipanah F,Khodagholi F,Maghsoudi N,Klenk HP

    更新日期：2010-05-01 00:00:00

  • DNA microarray technology for the microbiologist: an overview.

    abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-006-0584-2

    authors： Ehrenreich A

    更新日期：2006-11-01 00:00:00

  • Prophylactic effects of probiotic Bifidobacterium spp. in the resolution of inflammation in arthritic rats.

    abstract：:In the present study, the modulatory effects of bifidobacterial spp. (Bifidobacterium breve NCIM 5671, Bifidobacterium longum NCIM 5672 and Bifidobacterium bifidum NCIM 5697) on adjuvant induced arthritis in rats were evaluated. Arthritis was induced in male Wistar rats by injecting 250 μg of Freund's adjuvant directl...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-09864-2

    authors： Achi SC,Talahalli RR,Halami PM

    更新日期：2019-08-01 00:00:00

  • An efficient lipase-catalyzed enantioselective hydrolysis of (R,S)-azolides derived from N-protected proline, pipecolic acid, and nipecotic acid.

    abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4010-7

    authors： Wu CH,Pen CW,Wang PY,Tsai SW

    更新日期：2013-02-01 00:00:00

  • Effects of hydraulic retention time on aerobic granulation and granule growth kinetics at steady state with a fast start-up strategy.

    abstract：:A hydraulic retention time (HRT) of 4, 6, and 8 h was employed, respectively, in three reactors to study the effects of HRT on granulation with a newly developed fast granulation strategy, i.e., a strategy by combining strong hydraulic selection pressure with high organic loading rate (OLR). Granules with clear bounda...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6993-3

    authors： Liu YQ,Zhang X,Zhang R,Liu WT,Tay JH

    更新日期：2016-01-01 00:00:00

  • Molecular mechanism involved in the response to hydrogen peroxide stress in Acinetobacter oleivorans DR1.

    abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6914-5

    authors： Kim J,Cho Y,Jang IA,Park W

    更新日期：2015-12-01 00:00:00

  • Repeated application of carvone-induced bacteria to enhance biodegradation of polychlorinated biphenyls in soil.

    abstract：:Carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (PCB) by Arthrobacter sp. strain B1B. This study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of Aroclor-1242-contaminated soil. Control treatments compare...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051325

    authors： Gilbert ES,Crowley DE

    更新日期：1998-10-01 00:00:00

  • A homologue of cathepsin L identified in conditioned medium from Sf9 insect cells.

    abstract：:Gelatin zymography revealed the presence of proteolytic activity in conditioned medium (CM) from a serum-free, non-infected Spodoptera frugiperda, Sf9 insect cell culture. Two peptidase bands at about 49 and 39 kDa were detected and found to be proform and active form of the same enzyme. The 49-kDa form was visible on...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0181-9

    authors： Lindskog E,Svensson I,Häggström L

    更新日期：2006-07-01 00:00:00

  • Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method.

    abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-002-1108-3

    authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

    更新日期：2002-10-01 00:00:00

  • Bacterial community diversity associated with different levels of dietary nutrition in the rumen of sheep.

    abstract：:The sheep rumen microbial community plays an important role in animal performance and the environment. Few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. A total of 112 healthy Tan sheep were selected and randomly allotted to one of four dietary...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8144-5

    authors： Wang Y,Cao P,Wang L,Zhao Z,Chen Y,Yang Y

    更新日期：2017-05-01 00:00:00

  • A novel plasmid addiction system for large-scale production of cyanophycin in Escherichia coli using mineral salts medium.

    abstract：INTRODUCTION:Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase. M...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2899-2

    authors： Kroll J,Klinter S,Steinbüchel A

    更新日期：2011-02-01 00:00:00

  • Utilization of protein-rich residues in biotechnological processes.

    abstract：:A drawback of biotechnological processes, where microorganisms convert biomass constituents, such as starch, cellulose, hemicelluloses, lipids, and proteins, into wanted products, is the economic feasibility. Particularly the cost of nitrogen sources in biotechnological processes can make up a large fraction of total ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-015-7278-6

    authors： Pleissner D,Venus J

    更新日期：2016-03-01 00:00:00

  • Factors influencing antibiotic resistance burden in municipal wastewater treatment plants.

    abstract：:Municipal wastewater treatment plants are recognized reservoirs of antibiotic-resistant bacteria. Three municipal wastewater treatment plants differing on the dimensions and bio-treatment processes were compared for the loads of amoxicillin-, tetracycline-, and ciprofloxacin-resistant heterotrophic bacteria, enterobac...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2583-6

    authors： Novo A,Manaia CM

    更新日期：2010-07-01 00:00:00

  • Control of biofilm formation by poly-ethylene-co-vinyl acetate films incorporating nisin.

    abstract：:The aim of this study was to evaluate the effect of poly-ethylene-co-vinyl acetate (EVA) films incorporating different concentrations (0.1%, 0.5% and 1%) of nisin on the biofilm-forming ability of Listeria monocytogenes ATCC 7644, Staphylococcus aureus 815 and Staphylococcus epidermidis ATCC 35984. Nisin was incorpora...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2598-z

    authors： Nostro A,Scaffaro R,Ginestra G,D'Arrigo M,Botta L,Marino A,Bisignano G

    更新日期：2010-06-01 00:00:00

  • Microbiota composition of captive bluestreak cleaner wrasse Labroides dimidiatus (Valenciennes, 1839).

    abstract：:The Labroides dimidiatus is one of the most traded marine ornamental fishes worldwide, yet not much is known about the microflora associated with this fish. This study is designed to investigate the bacteria composition associated with captive L. dimidiatus and its surrounding aquarium water. The fish and carriage wat...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10781-y

    authors： Nurul AAN,Danish-Daniel AM,Okomoda VT,Asma NA

    更新日期：2020-09-01 00:00:00

  • Cloning, expression, and characterization of a synthetic analog to the bioadhesive precursor protein of the sea mussel Mytilus edulis.

    abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00228610

    authors： Salerno AJ,Goldberg I

    更新日期：1993-05-01 00:00:00

  • Comparative analysis of genetic diversity and expression of amoA in wastewater treatment processes.

    abstract：:The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1558-x

    authors： Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

    更新日期：2004-06-01 00:00:00

  • The role of N1 domain on the activity, stability, substrate specificity and raw starch binding of amylopullulanase of the extreme thermophile Geobacillus thermoleovorans.

    abstract：:In order to understand the role of N1 domain (1-257 aa) in the amylopullulanase (gt-apu) of the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion construct (gt-apuΔN) has been generated and expressed in Escherichia coli. The truncated amylopullulanase (gt-apuΔN) exhibits similar pH and tem...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6345-8

    authors： Nisha M,Satyanarayana T

    更新日期：2015-07-01 00:00:00

  • Expression of benzene dioxygenase from Pseudomonas putida ML2 in cis-1,2-cyclohexanediol-degrading pseudomonads.

    abstract：:Benzene dioxygenase (BDO; EC 1.14.12.3) from Pseudomonas putida ML2 dihydroxylates benzene to produce cis-1,2-dihydroxy-cyclohexa-3,5-diene. As well as oxidising benzene and toluene, cell-free extracts of Escherichia coli JM109 expressing recombinant BDO oxidised cyclohexene, 1-methylcyclohexene and 3-methylcyclohexen...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530100593

    authors： Swift RJ,Carter SF,Widdowson DA,Mason JR,Leak DJ

    更新日期：2001-06-01 00:00:00

  • Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles.

    abstract：:There is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. The use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . Additionally, there exists a need for the developmen...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-2032-6

    authors： Sintubin L,De Windt W,Dick J,Mast J,van der Ha D,Verstraete W,Boon N

    更新日期：2009-09-01 00:00:00

  • Induction of secondary metabolism of Aspergillus terreus ATCC 20542 in the batch bioreactor cultures.

    abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7157-1

    authors： Boruta T,Bizukojc M

    更新日期：2016-04-01 00:00:00

  • Studies on nutritional and oxygen requirements for production of L-asparaginase by Enterobacter aerogenes.

    abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530050006

    authors： Mukherjee J,Majumdar S,Scheper T

    更新日期：2000-02-01 00:00:00

  • Isolation and structural characterisation of two antibacterial free fatty acids from the marine diatom, Phaeodactylum tricornutum.

    abstract：:One solution to the global crisis of antibiotic resistance is the discovery of novel antimicrobial compounds for clinical application. Marine organisms are an attractive and, as yet, relatively untapped resource of new natural products. Cell extracts from the marine diatom, Phaeodactylum tricornutum, have antibacteria...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1714-9

    authors： Desbois AP,Lebl T,Yan L,Smith VJ

    更新日期：2008-12-01 00:00:00

  • Microbial production of 1,3-propanediol.

    abstract：:1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out by Clostridia as well as Enterobacteriaceae. The main intermediate of the oxidative pathway is pyruvate, the f...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s002530051523

    authors： Biebl H,Menzel K,Zeng AP,Deckwer WD

    更新日期：1999-09-01 00:00:00

  • Novel approaches and achievements in biosynthesis of functional isoprenoids in Escherichia coli.

    abstract：:Isoprenoids, also referred to as terpenes, are the most diverse class of natural products appearing in a variety of natural sources, specifically in higher plants, and have a wide range of biological functions. This review describes novel or recent approaches and achievements in pathway engineering of Escherichia coli...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-009-2166-6

    authors： Harada H,Misawa N

    更新日期：2009-10-01 00:00:00

  • Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent.

    abstract：:Polyhydroxyalkanoate (PHA) synthases catalyze chain transfer (CT) reaction after polymerization reaction of PHA by transferring PHA chain from PHA synthase to a CT agent, resulting in covalent bonding of CT agent to PHA chain at the carboxyl end. Previous studies have shown that poly(ethylene glycol) (PEG) is an effec...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2601-8

    authors： Tomizawa S,Saito Y,Hyakutake M,Nakamura Y,Abe H,Tsuge T

    更新日期：2010-07-01 00:00:00

  • Safe biotechnology. 8. Transport of infectious and biological materials. Working Party "Safety in Biotechnology" of the European Federation of Biotechnology.

    abstract：:The transport of infectious and biological material is regulated by a number of international organizations. This mini-review has been compiled to increase awareness within the scientific community of problems caused by differences in terminology (such as infectious materials/substances, biological products, diagnosti...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s002530051028

    authors： Lelieveld HL,Bachmayer H,Boon B,Bennett A,Brunius G,Bürki K,Cantley M,Collard JM,Collins CH,Crooy P,Doblhoff-Dier O,Dubakiene R,Economidis I,Elmqvist A,Frontali-Botti C,Havenaar R,Haymerle H,Käppeli O,Leaver G,Lex M

    更新日期：1997-08-01 00:00:00

  • Role of electrostatic interactions in cohesion of bacterial biofilms.

    abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-002-1044-2

    authors： Chen X,Stewart PS

    更新日期：2002-09-01 00:00:00