Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent.

abstract：:Corynebacterium glutamicum shows a great potential for the production of the polyamide monomer putrescine (1,4-diaminobutane). Previously, we constructed the putrescine-producing strain PUT1 by deletion of argF, the gene for ornithine transcarbamoylase (OTC), and argR, encoding the L-arginine repressor, combined with ...

journal_title：Applied microbiology and biotechnology

authors： Schneider J,Eberhardt D,Wendisch VF

更新日期：2012-07-01 00:00:00

abstract：:Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

journal_title：Applied microbiology and biotechnology

authors： Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

更新日期：2017-08-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:A series of continuous- and sequencing-batch reactor experiments were performed to assess the growth dynamics of Escherichia coli strain K12-MG1655 in chemostat systems. Previous mathematical predictions and early experimental results had shown that confined oscillatory dynamics ensue in bioreactor populations, which ...

journal_title：Applied microbiology and biotechnology

authors： Ofiţeru ID,Ferdeş M,Knapp CW,Graham DW,Lavric V

更新日期：2012-04-01 00:00:00

abstract：:Protein translation, translocation, folding, processing, and secretion in eukaryotic cells are complex and not always straightforward processes, e.g., different routes of secretion and degradation exist. Formation of malfolded proteins in the endoplasmic reticulum (ER) can be one of the major bottlenecks for recombina...

journal_title：Applied microbiology and biotechnology

authors： Pfeffer M,Maurer M,Stadlmann J,Grass J,Delic M,Altmann F,Mattanovich D

更新日期：2012-03-01 00:00:00

abstract：:The denitrifier community and associated nitrate and nitrite reduction in the bioaugmented and general sequencing batch reactors (SBRs) during the treatment of coking wastewater containing pyridine and quinoline were investigated. The efficiency and stability of nitrate and nitrite reduction in SBR was considerably im...

journal_title：Applied microbiology and biotechnology

authors： Bai Y,Sun Q,Xing R,Wen D,Tang X

更新日期：2011-05-01 00:00:00

abstract：:The main carbohydrate of red macroalgae is agarose, a heterogeneous polysaccharide composed of D-galactose and 3,6-anhydro-L-galactose. When saccharifying agarose by enzymes, the unique physical properties of agarose, namely the sol-gel transition and the near-insolubility of agarose in water, limit the accessibility ...

journal_title：Applied microbiology and biotechnology

authors： Kim JH,Yun EJ,Seo N,Yu S,Kim DH,Cho KM,An HJ,Kim JH,Choi IG,Kim KH

更新日期：2017-02-01 00:00:00

abstract：:Batch assays are currently used to study the kinetic behavior of microbial growth. However, it has been shown that the outcome of batch experiments is greatly influenced by the initial ratio of substrate concentration (S(o)) to biomass concentration (Xo). Substrate-sufficient batch culture is known to have mechanisms ...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Chen GH,Rols JL

更新日期：1999-11-01 00:00:00

abstract：:A maltotriose-producing α-amylase, AmyA, from a newly isolated bacterial strain Microbulbifer thermotolerans DAU221 was purified and characterized in the heterologous host, Escherichia coli, using the pCold I vector. The amyA gene encoded a 761-residue protein composed of a 33 amino acid secretion signal peptide. The ...

journal_title：Applied microbiology and biotechnology

authors： Lee YS,Park DJ,Choi YL

更新日期：2015-05-01 00:00:00

abstract：:Polyunsaturated fatty acids, docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3), and arachidonic acid (ARA, 20:4 n-6), have multiple beneficial effects on human health and can be used as an important ingredient in dietary supplements, food, feed and pharmaceuticals. A variety of microorganis...

journal_title：Applied microbiology and biotechnology

authors： Wu L,Roe CL,Wen Z

更新日期：2013-09-01 00:00:00

abstract：:Two sequencing batch reactors were synchronously operated to investigate the effect of manganese (II) (Mn(2+)) augmentation on aerobic granulation. Reactor 1 (R1) was added with 10 mg/L Mn(2+), while there was no Mn(2+) augmentation in reactor 2 (R2). Results showed that R1 had a faster granulation process than R2 and...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Yang T,Wang W,Zhang B,Sun Y

更新日期：2012-03-01 00:00:00

abstract：:A new and label-free capacitive immunosensor based on antibody-functionalized epoxysilane on a glassy carbon electrode has been developed for quantitative detection of human chorionic gonadotropin (hCG). Monitoring the changes in the capacitance signals of antibodies before and after the binding of the antigen provide...

journal_title：Applied microbiology and biotechnology

authors： Liao JY

更新日期：2007-04-01 00:00:00

abstract：:The objective of this study was to evaluate the effect of an embedded bio-nano hybrid system using nanoscale zinc oxide (n-ZnO) and lindane-degrading yeast Candida VITJzN04 for lindane degradation. Nano-embedding of the yeast was done with chemically synthesized n-ZnO particles (50 mg/mL) and was visualized by atomic ...

journal_title：Applied microbiology and biotechnology

authors： Salam JA,Das N

更新日期：2015-03-01 00:00:00

abstract：:Surfaces of massive chalcopyrite (CuFeS2) electrodes were modified by applying variable oxidation potential pulses under growth media in order to induce the formation of different secondary phases (e.g., copper-rich polysulfides, S n(2-); elemental sulfur, S(0); and covellite, CuS). The evolution of reactivity (oxidat...

journal_title：Applied microbiology and biotechnology

authors： Lara RH,García-Meza JV,González I,Cruz R

更新日期：2013-03-01 00:00:00

abstract：:Two extracellular enzymes (MsP1 and MsP2) capable of efficient beta-carotene degradation were purified from culture supernatants of the basidiomycete Marasmius scorodonius (garlic mushroom). Under native conditions, the enzymes exhibited molecular masses of approximately 150 and approximately 120 kDa, respectively. SD...

journal_title：Applied microbiology and biotechnology

authors： Scheibner M,Hülsdau B,Zelena K,Nimtz M,de Boer L,Berger RG,Zorn H

更新日期：2008-01-01 00:00:00

abstract：:The growing demand for sustainable animal production is compelling researchers to explore the potential approaches to reduce emissions of greenhouse gases from livestock that are mainly produced by enteric fermentation. Some potential solutions, for instance, the use of chemical inhibitors to reduce methanogenesis, ar...

journal_title：Applied microbiology and biotechnology

authors： Kumar S,Choudhury PK,Carro MD,Griffith GW,Dagar SS,Puniya M,Calabro S,Ravella SR,Dhewa T,Upadhyay RC,Sirohi SK,Kundu SS,Wanapat M,Puniya AK

更新日期：2014-01-01 00:00:00

abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

journal_title：Applied microbiology and biotechnology

authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

更新日期：2011-01-01 00:00:00

abstract：:Endophytic actinobacteria, which exist in the inner tissues of living plants, have attracted increasing attention among taxonomists, ecologists, agronomists, chemists and evolutionary biologists. Numerous studies have indicated that these prolific actinobacteria appear to have a capacity to produce an impressive array...

journal_title：Applied microbiology and biotechnology

authors： Qin S,Xing K,Jiang JH,Xu LH,Li WJ

更新日期：2011-02-01 00:00:00

abstract：:Hemidesmus indicus (L.) R. Br. ex Schult., commonly known as Indian sarsaparilla or Anantamul, is an ethnopharmacologically important medicinal plant from the family Apocynaceae. Biosynthesis of an array of bioactive compounds such as methoxybenzaldehydes and their derivatives has been accountable for its intensive me...

journal_title：Applied microbiology and biotechnology

authors： Nandy S,Singh J,Pandey DK,Dey A

更新日期：2020-10-01 00:00:00

abstract：:The present study emphasised on the development of new mathematical models based on mass balance and stoichiometry of nitrogen removal in anammox hybrid reactor (AHR). The performance of AHR at varying hydraulic retention times (HRTs) and nitrogen loading rates (NLRs) revealed that nitrogen removal efficiency (NRE) in...

journal_title：Applied microbiology and biotechnology

authors： Tomar S,Gupta SK

更新日期：2015-11-01 00:00:00

abstract：:Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical mice...

journal_title：Applied microbiology and biotechnology

authors： Mohanty S,Mukherji S

更新日期：2012-04-01 00:00:00

abstract：:Tributyl phosphate (TBP) is a toxic organophosphorous compound widely used in nuclear fuel processing and chemical industries. Rhodopseudomonas palustris, one of the most metabolically versatile photosynthetic bacteria, is shown here to degrade TBP efficiently under photosynthetic conditions. This study shows that thi...

journal_title：Applied microbiology and biotechnology

authors： Berne C,Pignol D,Lavergne J,Garcia D

更新日期：2007-11-01 00:00:00

abstract：:A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...

journal_title：Applied microbiology and biotechnology

authors： Herzog RW,Singh NK,Urry DW,Daniell H

更新日期：1997-04-01 00:00:00

abstract：:In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

journal_title：Applied microbiology and biotechnology

authors： Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

更新日期：2003-08-01 00:00:00

abstract：:In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...

journal_title：Applied microbiology and biotechnology

authors： Kim JY,Kim YG,Han YK,Choi HS,Kim YH,Lee GM

更新日期：2011-03-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous pr...

journal_title：Applied microbiology and biotechnology

authors： Gu Y,Zheng J,Feng J,Cao M,Gao W,Quan Y,Dang Y,Wang Y,Wang S,Song C

更新日期：2017-05-01 00:00:00

abstract：:Internal fragments of alpha- and beta-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5'- and 3'-rapid amplification of cDNA ends. Phytophthora capsici alpha- and beta-tubulin specific primers were then used to generate full-length cDNA b...

journal_title：Applied microbiology and biotechnology

authors： Koo BS,Park H,Kalme S,Park HY,Han JW,Yeo YS,Yoon SH,Kim SJ,Lee CM,Yoon MY

更新日期：2009-03-01 00:00:00

abstract：:Formate dehydrogenases (FDH) are useful for the regeneration of NADH, which is required for asymmetric reduction by several dehydrogenases and reductases. FDHs have relatively low activity and are labile, especially to alpha-haloketones, thus FDH cannot be applied to the industrial manufacture of optically active alph...

journal_title：Applied microbiology and biotechnology

authors： Yamamoto H,Mitsuhashi K,Kimoto N,Kobayashi Y,Esaki N

更新日期：2005-04-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00