Analysis of denitrifier community in a bioaugmented sequencing batch reactor for the treatment of coking wastewater containing pyridine and quinoline.


:The denitrifier community and associated nitrate and nitrite reduction in the bioaugmented and general sequencing batch reactors (SBRs) during the treatment of coking wastewater containing pyridine and quinoline were investigated. The efficiency and stability of nitrate and nitrite reduction in SBR was considerably improved after inoculation with four pyridine- or quinoline-degrading bacterial strains (including three denitrifying strains). Terminal restriction fragment length polymorphism (T-RFLP) based on the nosZ gene revealed that the structures of the denitrifier communities in bioaugmented and non-bioaugmented reactors were distinct and varied during the course of the experiment. Bioaugmentation protected indigenous denitrifiers from disruptions caused by pyridine and quinoline. Clone library analysis showed that one of the added denitrifiers comprised approximately 6% of the denitrifier population in the bioaugmented sludge.


Bai Y,Sun Q,Xing R,Wen D,Tang X




Has Abstract


2011-05-01 00:00:00












  • Synthesis of beta-D-glucosyl- and beta-D-fucosyl-glucoses using beta-glycosidase from Thermus thermophilus.

    abstract::The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Fourage L,Colas B

    更新日期:2001-08-01 00:00:00

  • Double Candida antarctica lipase B co-display on Pichia pastoris cell surface based on a self-processing foot-and-mouth disease virus 2A peptide.

    abstract::To develop a high efficiency Candida antarctica lipase B (CALB) yeast display system, we linked two CALB genes fused with Sacchromyces cerevisiae cell wall protein genes, the Sed1 and the 3'-terminal half of Sag1, separately by a 2A peptide of foot-and-mouth disease virus (FMDV) in a single open reading frame. The CAL...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Sun YF,Lin Y,Zhang JH,Zheng SP,Ye YR,Liang XX,Han SY

    更新日期:2012-12-01 00:00:00

  • A novel cell modification method used in biotransformation of glycerol to 3-HPA by Lactobacillus reuteri.

    abstract::The aim of the present study was to develop a new cell modification method to facilitate the cell separation from broth. In order to reduce the transfer limitation of substrate and product caused by general immobilization methods in the following biotransformation of glycerol, the carboxyl-functioned superparamagnetic...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Chen G,Chen J

    更新日期:2013-05-01 00:00:00

  • A new photobioreactor for continuous marennin production with a marine diatom: influence of the light intensity and the immobilised-cell matrix (alginate beads or agar layer).

    abstract::In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Lebeau T,Gaudin P,Moan R,Robert JM

    更新日期:2002-07-01 00:00:00

  • Identification of archaeon-producing hyperthermophilic alpha-amylase and characterization of the alpha-amylase.

    abstract::The extremely thermophilic anaerobic archaeon strain, HJ21, was isolated from a deep-sea hydrothermal vent, could produce hyperthermophilic alpha-amylase, and later was identified as Thermococcus from morphological, biochemical, and physiological characteristics and the 16S ribosomal RNA gene sequence. The extracellul...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wang S,Lu Z,Lu M,Qin S,Liu H,Deng X,Lin Q,Chen J

    更新日期:2008-09-01 00:00:00

  • Cloning, expression, and directed evolution of carbonyl reductase from Leifsonia xyli HS0904 with enhanced catalytic efficiency.

    abstract::(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol ((R)-BTPE) is a valuable chiral intermediate for the synthesis of antiemetic drug Aprepitant and Fosaprepitant. A Leifsonia xyli HS0904-derived carbonyl reductase (LXCAR), an effective biocatalyst for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone (BTAP) ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wang NQ,Sun J,Huang J,Wang P

    更新日期:2014-10-01 00:00:00

  • Efficient multi-enzyme-catalyzed CDP-choline production driven by an ATP donor module.

    abstract::Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

    更新日期:2017-02-01 00:00:00

  • Behaviour of dehydrated baker's yeast during reduction reactions in a biphasic medium.

    abstract::The behaviour of dry baker's yeast ( Saccharomyces cerevisiae type II, Sigma) used as biocatalyst without preliminary growth for the synthesis of 2-heptanol from 2-heptanone in a biphasic system is presented. Cells undergo intracellular trehalose consumption with a stoichiometric ethanol production during the first 15...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Cappaert L,Larroche C

    更新日期:2004-06-01 00:00:00

  • Opportunities for broadening the application of cell wall lytic enzymes.

    abstract::In light of emerging antibiotic resistance, bacterial cell wall lytic enzymes are promising antimicrobial agents that degrade bacterial peptidoglycan while specifically recognizing the target bacterium. The efficacy of lytic enzymes against several multi-drug-resistant pathogens infecting humans has led to many effort...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Bhagwat A,Mixon M,Collins CH,Dordick JS

    更新日期:2020-11-01 00:00:00

  • Performance of an acoustic settler versus a hollow fiber-based ATF technology for influenza virus production in perfusion.

    abstract::Process intensification and integration is crucial regarding an ever increasing pressure on manufacturing costs and capacities in biologics manufacturing. For virus production in perfusion mode, membrane-based alternating tangential flow filtration (ATF) and acoustic settler are the commonly described cell retention t...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Gränicher G,Coronel J,Trampler F,Jordan I,Genzel Y,Reichl U

    更新日期:2020-06-01 00:00:00

  • Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis.

    abstract::A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Seo MH,Kim KR,Oh DK

    更新日期:2013-10-01 00:00:00

  • Cytochromes P450 as promising catalysts for biotechnological application: chances and limitations.

    abstract::Cytochromes P450 (CYPs) belong to the superfamily of heme b containing monooxygenases with currently more than 21,000 members. These enzymes accept a vast range of organic molecules and catalyze diverse reactions. These extraordinary capabilities of CYP systems that are unmet by other enzymes make them attractive for ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Bernhardt R,Urlacher VB

    更新日期:2014-01-01 00:00:00

  • Effects of vanillin, quillaja saponin, and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen.

    abstract::This study investigated the effects of vanillin on methanogenesis and rumen fermentation, and the responses of ruminal protein-degrading bacteria to vanillin (at concentrations of 0, 0.76 and 1.52 g/L), essential oils (clove oil, 1 g/L; origanum oil, 0.50 g/L, and peppermint oil, 1 g/L), and quillaja saponin (at conce...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Patra AK,Yu Z

    更新日期:2014-01-01 00:00:00

  • Therapeutic potentials of short interfering RNAs.

    abstract::Short interfering RNA (siRNA) is one of the members of the family of RNA interference (RNAi). Coupled with the RNA-induced silencing complex (RISC), siRNA is able to trigger the cleavage of target RNAs which serve as a defensive system against pathogens. Meanwhile, siRNA in gene silencing opens a new avenue for the tr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Tam C,Wong JH,Cheung RCF,Zuo T,Ng TB

    更新日期:2017-10-01 00:00:00

  • A novel synthetic fluoro-containing jasmonate derivative acts as a chemical inducing signal for plant secondary metabolism.

    abstract::A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plan...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Qian ZG,Zhao ZJ,Xu Y,Qian X,Zhong JJ

    更新日期:2005-07-01 00:00:00

  • Protein engineering of toluene ortho-monooxygenase of Burkholderia cepacia G4 for regiospecific hydroxylation of indole to form various indigoid compounds.

    abstract::Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Rui L,Reardon KF,Wood TK

    更新日期:2005-01-01 00:00:00

  • A method to detect and quantify Phaeomoniella chlamydospora and Phaeoacremonium aleophilum DNA in grapevine-wood samples.

    abstract::Grapevines are sensitive to a wide range of fungal pathogens, including agents such as Phaeomoniella chlamydospora and Phaeoacremonium aleophilum that cause tracheomycosis. In the present study, a procedure for DNA extraction from grapevine woody tissue is first evaluated and shown to be suitable for quantitative anal...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Pouzoulet J,Mailhac N,Couderc C,Besson X,Daydé J,Lummerzheim M,Jacques A

    更新日期:2013-12-01 00:00:00

  • Internal loop photobiodegradation reactor (ILPBR) for accelerated degradation of sulfamethoxazole (SMX).

    abstract::The internal loop photobiodegradation reactor (ILPBR) was evaluated for the degradation of the pharmaceutical sulfamethoxazole (SMX) using batch experiments following three protocols: photolysis alone (P), biodegradation alone (B), and intimately coupled photolysis and biodegradation (P&B). SMX was removed more rapidl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Yan N,Xia S,Xu L,Zhu J,Zhang Y,Rittmann BE

    更新日期:2012-04-01 00:00:00

  • Enhanced vanillin production from ferulic acid using adsorbent resin.

    abstract::High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Hua D,Ma C,Song L,Lin S,Zhang Z,Deng Z,Xu P

    更新日期:2007-03-01 00:00:00

  • Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli.

    abstract::In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

    更新日期:2003-08-01 00:00:00

  • Transfer of megaplasmid pKB1 from the rubber-degrading bacterium Gordonia westfalica strain Kb1 to related bacteria and its modification.

    abstract::Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Bröker D,Arenskötter M,Steinbüchel A

    更新日期:2008-01-01 00:00:00

  • Overproduction of soluble recombinant transglutaminase from Streptomyces netropsis in Escherichia coli.

    abstract::A novel microbial transglutaminase (TGase) from the cultural filtrate of Streptomyces netropsis BCRC 12429 (Sn) was purified. The specific activity of the purified TGase was 18.2 U/mg protein with an estimated molecular mass of 38 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The TGase gen...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Yu YJ,Wu SC,Chan HH,Chen YC,Chen ZY,Yang MT

    更新日期:2008-12-01 00:00:00

  • Expression of a synthetic protein-based polymer (elastomer) gene in Aspergillus nidulans.

    abstract::A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Herzog RW,Singh NK,Urry DW,Daniell H

    更新日期:1997-04-01 00:00:00

  • Enhanced production of 5-hydroxytryptophan through the regulation of L-tryptophan biosynthetic pathway.

    abstract::5-Hydroxytryptophan (5-HTP) is the precursor of the neurotransmitter serotonin and has been used for the treatment of various diseases such as depression, insomnia, chronic headaches, and binge eating associated obesity. The production of 5-HTP had been achieved in our previous report, by the development of a recombin...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Xu D,Fang M,Wang H,Huang L,Xu Q,Xu Z

    更新日期:2020-03-01 00:00:00

  • Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau.

    abstract::Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) bacteria can play an important role in mitigating the methane emission produced in anoxic sediment layers to the atmosphere. However, the environmental factors regulating the distribution of these methane-oxidizing...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Liu Y,Zhang J,Zhao L,Li Y,Yang Y,Xie S

    更新日期:2015-03-01 00:00:00

  • β-glucosidase from Penicillium aculeatum hydrolyzes exo-, 3-O-, and 6-O-β-glucosides but not 20-O-β-glucoside and other glycosides of ginsenosides.

    abstract::A novel β-glucosidase from Penicillium aculeatum was purified as a single 110.5-kDa band on SDS-PAGE with a specific activity of 75.4 U mg⁻¹ by salt precipitation and Hi-Trap Q HP and Resource Q ion exchange chromatographies. The purified enzyme was identified as a member of the glycoside hydrolase 3 family based on i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Lee GW,Yoo MH,Shin KC,Kim KR,Kim YS,Lee KW,Oh DK

    更新日期:2013-07-01 00:00:00

  • Medicinal herbs as a potential strategy to decrease methane production by rumen microbiota: a systematic evaluation with a focus on Perilla frutescens seed extract.

    abstract::Mitigation of the methane (CH4) emission from ruminants is needed to decrease the environmental impact of ruminant animal production. Different plant materials and chemicals have been tested, but few are both effective and practical. Medicinal herbs contain biological compounds and antimicrobials that may be effective...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wang J,Liu M,Wu Y,Wang L,Liu J,Jiang L,Yu Z

    更新日期:2016-11-01 00:00:00

  • Generalizing a hybrid synthetic promoter approach in Yarrowia lipolytica.

    abstract::Both varied and strong promoters are essential for metabolic and pathway engineering applications in any host organism. To enable this capacity, here we demonstrate a generalizable method for the de novo construction of strong, synthetic hybrid promoter libraries. Specifically, we demonstrate how promoter truncation a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Blazeck J,Reed B,Garg R,Gerstner R,Pan A,Agarwala V,Alper HS

    更新日期:2013-04-01 00:00:00

  • Occurrence and function of enzymes for lignocellulose degradation in commercial Agaricus bisporus cultivation.

    abstract::The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Kabel MA,Jurak E,Mäkelä MR,de Vries RP

    更新日期:2017-06-01 00:00:00

  • Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor.

    abstract::Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxy...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Heyen U,Schüler D

    更新日期:2003-06-01 00:00:00