Abstract：
:With advances in the development of DNA vaccines and gene therapy, there is a growing need for plasmid DNA with high quality for fundamental research and clinical trials. In this report, a scalable automated process for large-scale preparation of plasmid is described. This process is based on alkaline lysis and can be easily scaled up to meet demands for larger quantities. In the process, harvested bacteria are passed through two mixing chambers at controlled speeds to affect lysis and control alkalinity. The resulting solution is passed through a series of filters to remove contaminants, and ethanol precipitated. System parameters are examined to maximize the quantity and quality of the prepared plasmid. Using this procedure, plasmid can be extracted and purified from 1 l of Escherichia coli cultures at an OD600 nm of 50 in less than 45 min. The plasmid yields are approximately 90 mg/l culture.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Li X,Jin H,Wu Z,Rayner S,Yin J,Yu Y,Zhang W,He Z,Wang C,Wang Bdoi
10.1007/s00253-007-0925-9subject
Has Abstractpub_date
2007-07-01 00:00:00pages
1217-23issue
5eissn
0175-7598issn
1432-0614journal_volume
75pub_type
杂志文章abstract：:During sporulation, Bacillus thuringiensis produces insecticidal crystal inclusions (Cry proteins) encoded by cry genes. In fed-batch cultures (FBCs), spores and Cry protein yields are usually low, so we therefore studied the pattern of metabolic changes occurring in batch cultures and FBCs of a B. thuringiensis strai...
journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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abstract：:The recent, rapid increase in bacterial antimicrobial resistance has become a major public health concern. One approach to generate new classes of antibacterials is targeting virulence rather than the viability of bacteria. Proteins of the Dsb system, which play a key role in the virulence of many pathogenic microorga...
journal_title：Applied microbiology and biotechnology
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abstract：:Biodiesel production by microalgae with photosynthetic CO2 biofixation is thought to be a feasible way in the field of bioenergy and carbon emission reduction. Knowledge of the carbon-concentrating mechanism plays an important role in improving microalgae carbon fixation efficiency. However, little information is avai...
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2014-09-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2015-11-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2012-04-01 00:00:00
abstract：:Protein translation, translocation, folding, processing, and secretion in eukaryotic cells are complex and not always straightforward processes, e.g., different routes of secretion and degradation exist. Formation of malfolded proteins in the endoplasmic reticulum (ER) can be one of the major bottlenecks for recombina...
journal_title：Applied microbiology and biotechnology
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更新日期：2012-03-01 00:00:00
abstract：:We used biodegradable plastics as fermentation substrates for the filamentous fungus Aspergillus oryzae. This fungus could grow under culture conditions that contained emulsified poly-(butylene succinate) (PBS) and emulsified poly-(butylene succinate-co-adipate) (PBSA) as the sole carbon source, and could digest PBS a...
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更新日期：2005-06-01 00:00:00
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doi：10.1007/s00253-015-6411-x
更新日期：2015-03-01 00:00:00
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更新日期：2010-07-01 00:00:00
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doi：10.1007/s00253-002-1162-x
更新日期：2003-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2014-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-013-5337-4
更新日期：2014-04-01 00:00:00
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更新日期：2004-11-01 00:00:00
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更新日期：2002-09-01 00:00:00
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更新日期：2020-08-01 00:00:00
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更新日期：2015-08-01 00:00:00
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abstract：:Biocides play an important role in the preservation of white mineral dispersions (WMD). Due to the occurrence of biocide-resistant bacteria and technical limitations in the use of biocides, new preservation strategies are required-like the enhancement of biocides by non-biocidal compounds. The aim of this study was to...
journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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更新日期：2007-03-01 00:00:00
