Improvement of protein production in lactic acid bacteria using 5'-untranslated leader sequence of slpA from Lactobacillus acidophilus. Improvement in protein production using UTLS.


:The 5'-untranslated leader sequence (UTLS) of the slpA gene from Lactobacillus acidophilus contributes to mRNA stabilization by producing a 5' stem and loop structure, and a high-level expression system for the lactic acid bacteria was developed using the UTLS in this study. A plasmid, which expresses alpha-amylase under the control of the ldh promoter, was constructed by integrating the core promoter sequence with the UTLS. The role of the UTLS in increasing the copies of the alpha-amylase mRNA was proved by measuring alpha-amylase activity in the culture supernatant and the relative expression of alpha-amylase mRNA was determined by the quantitative real-time PCR analysis. Moreover, several expression systems were constructed by combining the core promoter sequence with the UTLS or with the partially deleted UTLS and the expression level was evaluated. The use of the UTLS led to the success in improving alpha-amylase expression in the two strains of Lactobacillus casei and Lactococcus lactis. The current study showed that the improvement in protein production using the UTLS could be applied to the expression system in the lactic acid bacteria.


Narita J,Ishida S,Okano K,Kimura S,Fukuda H,Kondo A




Has Abstract


2006-11-01 00:00:00












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    pub_type: 杂志文章


    authors: Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

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    authors: Chavaroche AA,Springer J,Kooy F,Boeriu C,Eggink G

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    authors: Hofer H,Mandl T,Steiner W

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    authors: Schräder T,Andreesen JR

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    authors: Ferris CJ,Gilmore KG,Wallace GG,In het Panhuis M

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    authors: Bhushan B,Halasz A,Hawari J

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    authors: Ashby RD,Ngo HL,Solaiman DK,Strahan G

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    authors: Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

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    authors: Liu Y,Wang ZW,Qin L,Liu YQ,Tay JH

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    journal_title:Applied microbiology and biotechnology

    pub_type: 已发布勘误


    authors: Hauschild P,Röttig A,Madkour MH,Al-Ansari AM,Almakishah NH,Steinbüchel A

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    journal_title:Applied microbiology and biotechnology

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    authors: Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

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    authors: Kano H,Taguchi S,Momose H

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    pub_type: 杂志文章,评审


    authors: Murthy HN,Georgiev MI,Kim YS,Jeong CS,Kim SJ,Park SY,Paek KY

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    pub_type: 杂志文章,评审


    authors: Hassa J,Maus I,Off S,Pühler A,Scherer P,Klocke M,Schlüter A

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    pub_type: 杂志文章


    authors: Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

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    pub_type: 杂志文章


    authors: Xu Y,Zhang T,Xu Q,Han Z,Liang S,Shao Y,Ma D,Liu S

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    pub_type: 杂志文章


    authors: Scheibner M,Hülsdau B,Zelena K,Nimtz M,de Boer L,Berger RG,Zorn H

    更新日期:2008-01-01 00:00:00