Improvement of protein production in lactic acid bacteria using 5'-untranslated leader sequence of slpA from Lactobacillus acidophilus. Improvement in protein production using UTLS.


:The 5'-untranslated leader sequence (UTLS) of the slpA gene from Lactobacillus acidophilus contributes to mRNA stabilization by producing a 5' stem and loop structure, and a high-level expression system for the lactic acid bacteria was developed using the UTLS in this study. A plasmid, which expresses alpha-amylase under the control of the ldh promoter, was constructed by integrating the core promoter sequence with the UTLS. The role of the UTLS in increasing the copies of the alpha-amylase mRNA was proved by measuring alpha-amylase activity in the culture supernatant and the relative expression of alpha-amylase mRNA was determined by the quantitative real-time PCR analysis. Moreover, several expression systems were constructed by combining the core promoter sequence with the UTLS or with the partially deleted UTLS and the expression level was evaluated. The use of the UTLS led to the success in improving alpha-amylase expression in the two strains of Lactobacillus casei and Lactococcus lactis. The current study showed that the improvement in protein production using the UTLS could be applied to the expression system in the lactic acid bacteria.


Narita J,Ishida S,Okano K,Kimura S,Fukuda H,Kondo A




Has Abstract


2006-11-01 00:00:00












  • Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase.

    abstract::Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

    更新日期:2013-08-01 00:00:00

  • Biotin-mediated growth and gene expression in Staphylococcus aureus is highly responsive to environmental biotin.

    abstract::Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Satiaputra J,Eijkelkamp BA,McDevitt CA,Shearwin KE,Booker GW,Polyak SW

    更新日期:2018-04-01 00:00:00

  • Understanding the mechanisms of zinc bacitracin and avilamycin on animal production: linking gut microbiota and growth performance in chickens.

    abstract::Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production. Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Crisol-Martínez E,Stanley D,Geier MS,Hughes RJ,Moore RJ

    更新日期:2017-06-01 00:00:00

  • Batch and continuous cultivation of Anaerobiospirillum succiniciproducens for the production of succinic acid from whey.

    abstract::Batch and continuous cultivation of Anaerobiospirillum succiniciproducens were systematically studied for the production of succinic acid from whey. Addition of 2.5 g l(-1) yeast extract and 2.5 g l(-1) polypeptone per 10 g l(-1) whey was most effective for succinic acid production from both treated and nontreated whe...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Lee PC,Lee WG,Kwon S,Lee SY,Chang HN

    更新日期:2000-07-01 00:00:00

  • Effect of glucose feeding on the glycosylation quality of antibody produced by a human cell line, F2N78, in fed-batch culture.

    abstract::The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

    更新日期:2014-04-01 00:00:00

  • Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples.

    abstract::Ethidium monoazide bromide (EMA) treatment of pure culture and environmental waters at low concentrations (1.0-7.5 microg/ml) indicated effective enumeration of viable and viable but nonculturable Escherichia coli in pure cultures, creek waters, and secondary activated sludge effluent samples by quantitative polymeras...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Gedalanga PB,Olson BH

    更新日期:2009-03-01 00:00:00

  • Application of the FLP/FRT recombination system in cyanobacteria for construction of markerless mutants.

    abstract::Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Tan X,Liang F,Cai K,Lu X

    更新日期:2013-07-01 00:00:00

  • Cellulase recycling in biorefineries--is it possible?

    abstract::On a near future, bio-based economy will assume a key role in our lives. Lignocellulosic materials (e.g., agroforestry residues, industrial/solid wastes) represent a cheaper and environmentally friendly option to fossil fuels. Indeed, following suitable processing, they can be metabolized by different microorganisms t...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Gomes D,Rodrigues AC,Domingues L,Gama M

    更新日期:2015-05-01 00:00:00

  • Genome-centered omics insight into the competition and niche differentiation of Ca. Jettenia and Ca. Brocadia affiliated to anammox bacteria.

    abstract::Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhao Y,Feng Y,Chen L,Niu Z,Liu S

    更新日期:2019-10-01 00:00:00

  • Bioflavoring and beer refermentation.

    abstract::Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

    更新日期:2003-08-01 00:00:00

  • Biotechnological production of eleutherosides: current state and perspectives.

    abstract::Eleutherosides, the phenylpropanoid and lignan glycosides, are the active ingredients accumulated in the roots and stems of Eleutherococcus species and in Eleutherococcus senticosus in particular. Syringin (=eleutheroside B) and (-) syringaresinol-di-O-β-D-glucoside (=eleutheroside E) appear as the most important bioa...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Murthy HN,Kim YS,Georgiev MI,Paek KY

    更新日期:2014-09-01 00:00:00

  • Efficient secreted production of (R)-3-hydroxybutyric acid from living Halomonas sp. KM-1 under successive aerobic and microaerobic conditions.

    abstract::Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB). The second microaerobic condition, achieved by reducing the cult...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kawata Y,Kawasaki K,Shigeri Y

    更新日期:2012-11-01 00:00:00

  • Expression of a catalytic domain of a Neocallimastix frontalis endoxylanase gene (xyn3) in Kluyveromyces lactis and Penicillium roqueforti.

    abstract::A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Durand R,Rascle C,Fèvre M

    更新日期:1999-08-01 00:00:00

  • Use of azo dye ligand chromatography for the partial purification of a novel extracellular peroxidase from Streptomyces viridosporus T7A.

    abstract::Crude peroxidase preparations from the lignocellulose-degrading actinomycete, Streptomyces viridosporus T7A, were shown to decolorize several azo dye isomers and showed a correlation of dye structure to degradability similar to that shown by fungal Mn-peroxidase, an enzyme not previously described in actinomycetes. Ad...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Burke NS,Crawford DL

    更新日期:1998-05-01 00:00:00

  • Fungal biotransformation of benzo[f]quinoline, benzo[h]quinoline, and phenanthridine.

    abstract::Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

    更新日期:2005-05-01 00:00:00

  • Immunoaffinity layering of enzymes. Stabilization and use in flow injection analysis of glucose and hydrogen peroxide.

    abstract::A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described. Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated by ammoni...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Farooqi M,Sosnitza P,Saleemuddin M,Ulber R,Scheper T

    更新日期:1999-09-01 00:00:00

  • Asparaginase inhibition of adhesion of type 1-fimbriated and P-fimbriated Escherichia coli to epithelial cell receptors.

    abstract::The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

    更新日期:2004-11-01 00:00:00

  • Expression of benzene dioxygenase from Pseudomonas putida ML2 in cis-1,2-cyclohexanediol-degrading pseudomonads.

    abstract::Benzene dioxygenase (BDO; EC from Pseudomonas putida ML2 dihydroxylates benzene to produce cis-1,2-dihydroxy-cyclohexa-3,5-diene. As well as oxidising benzene and toluene, cell-free extracts of Escherichia coli JM109 expressing recombinant BDO oxidised cyclohexene, 1-methylcyclohexene and 3-methylcyclohexen...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Swift RJ,Carter SF,Widdowson DA,Mason JR,Leak DJ

    更新日期:2001-06-01 00:00:00

  • A thermophilic-like ene-reductase originating from an acidophilic iron oxidizer.

    abstract::Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

    更新日期:2017-01-01 00:00:00

  • Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein.

    abstract::Previous studies have shown that a 20-kDa protein enhances production of the insecticidal CytA and CryIVA proteins of Bacillus thuringiensis in Escherichia coli as well as CytA production and crystal formation in B. thuringiensis. To determine whether the 20-kDa protein could enhance CryIVD production, an expression v...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wu D,Federici BA

    更新日期:1995-01-01 00:00:00

  • Studies on the inactivation of the flavoprotein D-amino acid oxidase from Trigonopsis variabilis.

    abstract::Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Schräder T,Andreesen JR

    更新日期:1996-05-01 00:00:00

  • Microbial population dynamics and proteomics in membrane bioreactors with enzymatic quorum quenching.

    abstract::Quorum sensing gives rise to biofilm formation on the membrane surface, which in turn causes a loss of water permeability in membrane bioreactors (MBRs) for wastewater treatment. Enzymatic quorum quenching was reported to successfully inhibit the formation of biofilm in MBRs through the decomposition of signal molecul...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kim HW,Oh HS,Kim SR,Lee KB,Yeon KM,Lee CH,Kim S,Lee JK

    更新日期:2013-05-01 00:00:00

  • Advances in biofilm reactors for production of value-added products.

    abstract::Biofilms are defined as microbial cell layers, which are irreversibly or reversibly attached on solid surfaces. These attached cells are embedded in a self-produced exopolysaccharide matrix, and exhibit different growth and bioactivity compared with suspended cells. With their high biomass density, stability, and pote...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Cheng KC,Demirci A,Catchmark JM

    更新日期:2010-06-01 00:00:00

  • Structure/function relationship of homopolysaccharide producing glycansucrases and therapeutic potential of their synthesised glycans.

    abstract::The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Korakli M,Vogel RF

    更新日期:2006-08-01 00:00:00

  • Microbiota composition of captive bluestreak cleaner wrasse Labroides dimidiatus (Valenciennes, 1839).

    abstract::The Labroides dimidiatus is one of the most traded marine ornamental fishes worldwide, yet not much is known about the microflora associated with this fish. This study is designed to investigate the bacteria composition associated with captive L. dimidiatus and its surrounding aquarium water. The fish and carriage wat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Nurul AAN,Danish-Daniel AM,Okomoda VT,Asma NA

    更新日期:2020-09-01 00:00:00

  • Methyl jasmonate elicitation enhanced synthesis of ginsenoside by cell suspension cultures of Panax ginseng in 5-l balloon type bubble bioreactors.

    abstract::The effects of methyl jasmonate (MJ) elicitation on the cell growth and accumulation of ginsenoside in 5-l bioreactor suspension cultures of Panax ginseng were investigated. Ginsenoside accumulation was enhanced by elicitation by MJ (in the range 50-400 microM); however, fresh weight, dry weight and growth ratio of th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Thanh NT,Murthy HN,Yu KW,Hahn EJ,Paek KY

    更新日期:2005-04-01 00:00:00

  • Surface display of active lipases Lip7 and Lip8 from Yarrowia lipolytica on Saccharomyces cerevisiae.

    abstract::Lipase has been used widely in industry. In this study, we have constructed two recombinant Saccharomyces cerevisiae strains displaying two active lipases on the cell surface by cell surface engineering. The genes encoding Yarrowia lipolytica lipases Lip7 and Lip8 were fused with the gene encoding small binding subuni...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Liu WS,Pan XX,Jia B,Zhao HY,Xu L,Liu Y,Yan YJ

    更新日期:2010-10-01 00:00:00

  • BioBrick™ compatible vector system for protein expression in Rhodobacter sphaeroides.

    abstract::We report here the creation of a modular, plasmid-based protein expression system utilizing elements of the native Rhodobacter puf promoter in a BioBrick(TM)-based vector system with DsRed encoding a red fluorescent reporter protein. A suite of truncations of the puf promoter were made to assess the influence of diffe...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Tikh IB,Held M,Schmidt-Dannert C

    更新日期:2014-04-01 00:00:00

  • Functional analysis of the dehydratase domains of a PUFA synthase from Thraustochytrium in Escherichia coli.

    abstract::Thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (VLC-PUFAs), by a polyunsaturated fatty acid (PUFA) synthase comprising three large subunits with multiple catalytic dehydratase (DH) domains critical for introducing double...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Xie X,Meesapyodsuk D,Qiu X

    更新日期:2018-01-01 00:00:00

  • Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor.

    abstract::Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxy...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Heyen U,Schüler D

    更新日期:2003-06-01 00:00:00