Coordinated activation of PTA-ACS and TCA cycles strongly reduces overflow metabolism of acetate in Escherichia coli.


:Elimination of acetate overflow in aerobic cultivation of Escherichia coli would improve many bioprocesses as acetate accumulation in the growth environment leads to numerous negative effects, e.g. loss of carbon, inhibition of growth, target product synthesis, etc. Despite many years of studies, the mechanism and regulation of acetate overflow are still not completely understood. Therefore, we studied the growth of E. coli K-12 BW25113 and several of its mutant strains affecting acetate-related pathways using the continuous culture method accelerostat (A-stat) at various specific glucose consumption rates with the aim of diminishing acetate overflow. Absolute quantitative exo-metabolome and proteome analyses coupled to metabolic flux analysis enabled us to demonstrate that onset of acetate overflow can be postponed and acetate excretion strongly reduced in E. coli by coordinated activation of phosphotransacetylase-acetyl-CoA synthetase (PTA-ACS) and tricarboxylic acid (TCA) cycles. Fourfold reduction of acetate excretion (2 vs. 8 % from total carbon) at fastest growth compared to wild type was achieved by deleting the genes responsible for inactivation of acetyl-CoA synthetase protein (pka) and TCA cycle regulator arcA. The Δpka ΔarcA strain did not accumulate any other detrimental by-product besides acetate and showed identical μ max and only ~5 % lower biomass yield compared to wild type. We conclude that a fine-tuned coordination between increasing the recycling capabilities of acetate in the PTA-ACS node through a higher concentration of active acetate scavenging Acs protein and downstream metabolism throughput in the TCA cycle is necessary for diminishing overflow metabolism of acetate in E. coli and achieving higher target product production in bioprocesses.


Peebo K,Valgepea K,Nahku R,Riis G,Oun M,Adamberg K,Vilu R




Has Abstract


2014-06-01 00:00:00












  • New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Moldes C,Farinós GP,de Eugenio LI,García P,García JL,Ortego F,Hernández-Crespo P,Castañera P,Prieto MA

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  • Biotin-mediated growth and gene expression in Staphylococcus aureus is highly responsive to environmental biotin.

    abstract::Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Satiaputra J,Eijkelkamp BA,McDevitt CA,Shearwin KE,Booker GW,Polyak SW

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  • Generation of biologically contained, readily transformable, and genetically manageable mutants of the biotechnologically important Bacillus pumilus.

    abstract::Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wemhoff S,Meinhardt F

    更新日期:2013-09-01 00:00:00

  • Application of locked nucleic acid-based probes in fluorescence in situ hybridization.

    abstract::Fluorescence in situ hybridization (FISH) employing nucleic acid mimics as probes is becoming an emerging molecular tool in the microbiology area for the detection and visualization of microorganisms. However, the impact that locked nucleic acid (LNA) and 2'-O-methyl (2'-OMe) RNA modifications have on the probe that i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Fontenete S,Carvalho D,Guimarães N,Madureira P,Figueiredo C,Wengel J,Azevedo NF

    更新日期:2016-07-01 00:00:00

  • Azoreductase from alkaliphilic Bacillus sp. AO1 catalyzes indigo reduction.

    abstract::Indigo is an insoluble blue dye historically used for dyeing textiles. A traditional approach for indigo dyeing involves microbial reduction of polygonum indigo to solubilize it under alkaline conditions; however, the mechanism by which microorganisms reduce indigo remains poorly understood. Here, we aimed to identify...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Suzuki H,Abe T,Doi K,Ohshima T

    更新日期:2018-11-01 00:00:00

  • Heterologous signal peptides-directing secretion of Streptomyces mobaraensis transglutaminase by Bacillus subtilis.

    abstract::Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Mu D,Lu J,Qiao M,Kuipers OP,Zhu J,Li X,Yang P,Zhao Y,Luo S,Wu X,Jiang S,Zheng Z

    更新日期:2018-07-01 00:00:00

  • Cellular fatty acid profile and H(+)-ATPase activity to assess acid tolerance of Bacillus sp. for potential probiotic functional attributes.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Shobharani P,Halami PM

    更新日期:2014-11-01 00:00:00

  • Synthesis of beta-D-glucosyl- and beta-D-fucosyl-glucoses using beta-glycosidase from Thermus thermophilus.

    abstract::The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Fourage L,Colas B

    更新日期:2001-08-01 00:00:00

  • Biodegradation and bioremediation of hydrocarbons in extreme environments.

    abstract::Many hydrocarbon-contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, high salt concentrations, or high pressure, Hydrocarbon-degrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Margesin R,Schinner F

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  • Identification and quantification of Thiothrix eikelboomii using qPCR for early detection of bulking incidents in a full-scale water reclamation plant.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Asvapathanagul P,Olson BH,Gedalanga PB,Hashemi A,Huang Z,La J

    更新日期:2015-05-01 00:00:00

  • Roles of three TonB systems in the iron utilization and virulence of the Aeromonas hydrophila Chinese epidemic strain NJ-35.

    abstract::The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the r...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Dong Y,Geng J,Liu J,Pang M,Awan F,Lu C,Liu Y

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  • Nitrogen source and mineral optimization enhance D: -xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Slininger PJ,Dien BS,Gorsich SW,Liu ZL

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  • The occurrence of killer, sensitive, and neutral yeasts in Brazilian Riesling Italico grape must and the effect of neutral strains on killing behaviour.

    abstract::The occurrence of killer toxins amongst yeast in Brazilian Riesling Italico grape must was investigated by using the sensitive strain EMBRAPA-26B as a reference strain at 18 degrees C and 28 degrees C. From a total of 85 previously isolated yeasts, 21 strains showed ability to kill the sensitive strain on unbuffered g...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: da Silva GA

    更新日期:1996-09-01 00:00:00

  • Soybean transcription factor GmMYBZ2 represses catharanthine biosynthesis in hairy roots of Catharanthus roseus.

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    journal_title:Applied microbiology and biotechnology

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    authors: Zhou ML,Hou HL,Zhu XM,Shao JR,Wu YM,Tang YX

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    journal_title:Applied microbiology and biotechnology

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    authors: Zhang L,Huang Y,Xu D,Yang L,Qian K,Chang G,Gong Y,Zhou X,Ma K

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  • A lab-scale model system for cocoa bean fermentation.

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    journal_title:Applied microbiology and biotechnology

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    authors: Romanens E,Näf R,Lobmaier T,Pedan V,Leischtfeld SF,Meile L,Schwenninger SM

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  • A novel method for RNA extraction from Andosols using casein and its application to amoA gene expression study in soil.

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    journal_title:Applied microbiology and biotechnology

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    authors: Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

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  • A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Libert X,Chasseur C,Packeu A,Bureau F,Roosens NH,De Keersmaecker SJC

    更新日期:2016-02-01 00:00:00

  • The treatment of gaseous benzene by two-phase partitioning bioreactors: a high performance alternative to the use of biofilters.

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    journal_title:Applied microbiology and biotechnology

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    authors: Davidson CT,Daugulis AJ

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  • Fluorescence-based high-throughput screening system for R-ω-transaminase engineering and its substrate scope extension.

    abstract::ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA) limits its application in R-amine synthesis. A fluorescence-based TA activity screening system was develo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Cheng F,Chen XL,Xiang C,Liu ZQ,Wang YJ,Zheng YG

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  • Characterization of two Listeria innocua chitinases of different sizes that were expressed in Escherichia coli.

    abstract::Two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium Listeria innocua were expressed in Escherichia coli, and the gene products were characterized. The genes were close homologs of chitinases from the pathogenic bacterium Listeria monocytogenes, in which chitinases and chitin-binding pro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Honda S,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

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  • The solvent efflux system of Pseudomonas putida S12 is not involved in antibiotic resistance.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Isken S,De Bont JA

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  • Protein engineering of toluene ortho-monooxygenase of Burkholderia cepacia G4 for regiospecific hydroxylation of indole to form various indigoid compounds.

    abstract::Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Rui L,Reardon KF,Wood TK

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Hirano J,Miyamoto K,Ohta H

    更新日期:2008-08-01 00:00:00

  • A freshwater bacterial strain, Shewanella sp. Lzh-2, isolated from Lake Taihu and its two algicidal active substances, hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 2, 3-indolinedione.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Li Z,Lin S,Liu X,Tan J,Pan J,Yang H

    更新日期:2014-05-01 00:00:00

  • Impact of dibenzofuran/dibenzo-p-dioxin amendment on bacterial community from forest soil and ring-hydroxylating dioxygenase gene populations.

    abstract::The impact of dibenzofuran (DF) and dibenzo-p-dioxin (DD) on the changes in bacterial community structure and the transition of catabolic genes were studied using forest soil. The bacterial community structure of soil suspensions amended with 1 microg/g of either DF or DD was analyzed by 16S rRNA and functional gene s...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kimura N,Kamagata Y

    更新日期:2009-08-01 00:00:00

  • Evaluation of white-rot fungi for detoxification and decolorization of effluents from the green olive debittering process.

    abstract::Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi ( Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus an...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Aggelis G,Ehaliotis C,Nerud F,Stoychev I,Lyberatos G,Zervakis GI

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  • Xylose anaerobic conversion by open-mixed cultures.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Temudo MF,Mato T,Kleerebezem R,van Loosdrecht MC

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Yasueda H,Kikuchi Y,Kojima H,Nagase K

    更新日期:1991-11-01 00:00:00

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Bhagwat A,Mixon M,Collins CH,Dordick JS

    更新日期:2020-11-01 00:00:00