Steroid phosphate esters and phosphonosteroids and their biological activities.

abstract：:Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli c...

journal_title：Applied microbiology and biotechnology

authors： Yang H,Hu J,Lu X,Wang F,Shen W,Hu W,Wang L,Chen X,Liu L

更新日期：2019-01-01 00:00:00

abstract：:Brazzein is an intensely sweet-tasting plant protein with good stability, which makes it an attractive alternative to sucrose. A brazzein gene has been designed, synthesized, and expressed in Escherichia coli at 30 degrees C to yield brazzein in a soluble form and in considerable quantity. Antibodies have been produce...

journal_title：Applied microbiology and biotechnology

authors： Berlec A,Jevnikar Z,Majhenic AC,Rogelj I,Strukelj B

更新日期：2006-11-01 00:00:00

abstract：:In this study, we demonstrated that anaerobic granular sludge could be successfully transformed into aerobic granular sludge in a continuous up-flow reactor in 45 days. An aerobic microbial community successfully developed in the granules and high organic matter and nitrogen removal performance was achieved. Under an ...

journal_title：Applied microbiology and biotechnology

authors： Sun H,Yu P,Li Q,Ren H,Liu B,Ye L,Zhang XX

更新日期：2017-10-01 00:00:00

abstract：:The sheep rumen microbial community plays an important role in animal performance and the environment. Few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. A total of 112 healthy Tan sheep were selected and randomly allotted to one of four dietary...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Cao P,Wang L,Zhao Z,Chen Y,Yang Y

更新日期：2017-05-01 00:00:00

abstract：:Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes...

journal_title：Applied microbiology and biotechnology

authors： Chen CH,Tu CC,Kuo HY,Zeng RF,Yu CS,Lu HH,Liou ML

更新日期：2017-01-01 00:00:00

abstract：:The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. I...

journal_title：Applied microbiology and biotechnology

authors： Kakeshita H,Kageyama Y,Ara K,Ozaki K,Nakamura K

更新日期：2011-03-01 00:00:00

abstract：:Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

journal_title：Applied microbiology and biotechnology

authors： Rui L,Reardon KF,Wood TK

更新日期：2005-01-01 00:00:00

abstract：:Currently, increasing attention cancer treatment has focused on molecularly targeted therapies and more recently on immunotherapies targeting immune checkpoints. However, even such advanced treatment may be ineffective. The reasons for this are sought, inter alia, in the human microbiome. In our intestines, there are ...

journal_title：Applied microbiology and biotechnology

authors： Grenda A,Krawczyk P

更新日期：2021-01-25 00:00:00

abstract：:Thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (VLC-PUFAs), by a polyunsaturated fatty acid (PUFA) synthase comprising three large subunits with multiple catalytic dehydratase (DH) domains critical for introducing double...

journal_title：Applied microbiology and biotechnology

authors： Xie X,Meesapyodsuk D,Qiu X

更新日期：2018-01-01 00:00:00

abstract：:Since the last 20 years, bacteria of the genus Acinetobacter have been the leading cause of hospital-acquired infections. In addition to the ability of Acinetobacter species to acquire rapid antibiotic resistance, limited knowledge on the mechanisms of multidrug resistance to antibiotics limits the treatment options f...

journal_title：Applied microbiology and biotechnology

authors： Shin B,Park C,Park W

更新日期：2020-02-01 00:00:00

abstract：:Nonribosomal peptides (NRPs), a large family of natural products, possess numerous pharmaceutically significant bioactivities. However, many native microbial producers of NRPs are not cultivable or have low production yields making mass production infeasible. The recombinant production of natural products in a surroga...

journal_title：Applied microbiology and biotechnology

authors： Li J,Jaitzig J,Hillig F,Süssmuth R,Neubauer P

更新日期：2014-01-01 00:00:00

abstract：:Population dynamics was studied in a 52-l biotrickling filter (BTF) operated for 182 days and used to clean air contaminated with styrene vapors. In the BTF, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. PCR-amplified 16S rDNA fragments from planktonic and sessile cells within...

journal_title：Applied microbiology and biotechnology

authors： Tresse O,Lorrain MJ,Rho D

更新日期：2002-08-01 00:00:00

abstract：:Validamycin A (VAL-A) is a widely used antifungal antibiotic for the treatment of sheath blight disease of rice and other plants. It can be produced from agro-industrial by-products by Streptomyces hygroscopicus 5008. To enhance its production titer, in this work, the entire val gene cluster was amplified in tandem in...

journal_title：Applied microbiology and biotechnology

authors： Zhou TC,Kim BG,Zhong JJ

更新日期：2014-09-01 00:00:00

abstract：:Alcaligenes sp. A 7-2 immobilized on granular clay has been applied in a percolator to degrade 4-chlorophenol in sandy soil. Good adsorption rates on granular clay were achieved using cell suspensions with high titres and media at pH 8.0. The influence of various parameters such as aeration rate, pH, temperature, conc...

journal_title：Applied microbiology and biotechnology

authors： Balfanz J,Rehm HJ

更新日期：1991-08-01 00:00:00

abstract：:There is currently an increasing number of cytochrome P450 (CYP450) monooxygenase encoding genes becoming available from various genome-sequencing projects. These enzymes require association with cytochrome P450 reductase (CPR) to achieve optimal activities. In this study, the CYP53B1 gene, which encodes a benzoate pa...

journal_title：Applied microbiology and biotechnology

authors： Shiningavamwe A,Obiero G,Albertyn J,Nicaud JM,Smit M

更新日期：2006-09-01 00:00:00

abstract：:The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. ...

journal_title：Applied microbiology and biotechnology

authors： D'Orazio G,Di Gennaro P,Boccarusso M,Presti I,Bizzaro G,Giardina S,Michelotti A,Labra M,La Ferla B

更新日期：2015-11-01 00:00:00

abstract：:Immunostimulatory glucose polymers known as beta-glucans have been studied for many years. Our laboratory has prepared and characterized a novel microparticulate beta-glucan (MG) from the budding yeast Saccharomyces cerevisiae. Because MG particles are rapidly phagocytized by murine peritoneal macrophages and induce t...

journal_title：Applied microbiology and biotechnology

authors： Berner VK,Sura ME,Hunter KW Jr

更新日期：2008-10-01 00:00:00

abstract：:Stable transformants of Chromobacterium violaceum were obtained by high-voltage electroporation with a 7-kilobase binary plasmid. The technique was reliable, reproducible, and simple, with efficiencies of 10(5) transformants/microg of plasmid DNA. The electrical conditions that resulted in the highest efficiencies wer...

journal_title：Applied microbiology and biotechnology

authors： Broetto L,Cecagno R,Sant'anna FH,Weber S,Schrank IS

更新日期：2006-07-01 00:00:00

abstract：:Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

journal_title：Applied microbiology and biotechnology

authors： Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

更新日期：2003-08-01 00:00:00

abstract：:Saccharomyces bayanus, being of interest for wine-making, is not as well known as S. cerevisiae and, due to many changes in the yeast classification, accurate data concerning its metabolic activity are difficult to find. In order to produce this yeast as an active dry yeast to be used as a starter in wine-making, its ...

journal_title：Applied microbiology and biotechnology

authors： Serra A,Strehaiano P,Taillandier P

更新日期：2003-10-01 00:00:00

abstract：:By damaging DNA molecules, genotoxicants cause genetic mutations and also increase human susceptibility to cancers and genetic diseases. Over the past four decades, several assays have been developed in the budding yeast Saccharomyces cerevisiae to screen potential genotoxic substances and provide alternatives to anim...

journal_title：Applied microbiology and biotechnology

authors： Eki T

更新日期：2018-03-01 00:00:00

abstract：:L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

journal_title：Applied microbiology and biotechnology

authors： Ochsenreither K,Fischer C,Neumann A,Syldatk C

更新日期：2014-06-01 00:00:00

abstract：:Lantibiotic nukacin ISK-1 is produced by Staphylococcus warneri ISK-1. The dual functional transporter NukT, an ABC transporter maturation and secretion protein, contributes to cleavage of the leader peptide from the prepeptide (modified NukA) and the final transport of nukacin ISK-1. NukT consists of an N-terminal pe...

journal_title：Applied microbiology and biotechnology

authors： Zheng S,Nagao JI,Nishie M,Zendo T,Sonomoto K

更新日期：2018-01-01 00:00:00

abstract：:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechoc...

journal_title：Applied microbiology and biotechnology

authors： Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

更新日期：2008-03-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are sti...

journal_title：Applied microbiology and biotechnology

authors： Ye X,Yang L,Jia J,Han J,Li S,Liu Y,Xu L,Zhao H,Chen Y,Li Y,Cheng T,Xia N

更新日期：2016-03-01 00:00:00

abstract：:Four potential dehydrogenases identified through literature and bioinformatic searches were tested for L-arabonate production from L-arabinose in the yeast Saccharomyces cerevisiae. The most efficient enzyme, annotated as a D-galactose 1-dehydrogenase from the pea root nodule bacterium Rhizobium leguminosarum bv. trif...

journal_title：Applied microbiology and biotechnology

authors： Aro-Kärkkäinen N,Toivari M,Maaheimo H,Ylilauri M,Pentikäinen OT,Andberg M,Oja M,Penttilä M,Wiebe MG,Ruohonen L,Koivula A

更新日期：2014-12-01 00:00:00

abstract：:Sulfide detoxification can be catalyzed by ancient membrane-bound flavoproteins, sulfide:quinone oxidoreductases (Sqr), which have important roles in sulfide homeostasis and sulfide-dependent energy conservation processes by transferring electrons from sulfide to respiratory or photosynthetic membrane electron flow. S...

journal_title：Applied microbiology and biotechnology

authors： Duzs Á,Tóth A,Németh B,Balogh T,Kós PB,Rákhely G

更新日期：2018-06-01 00:00:00

abstract：:Acid tolerance and aroma profile are crucial factors for wine production in Saccharomyces cerevisiae. However, most wine yeasts to date fail to endure low-pH environments, therefore resulting in problems such as lengthened fermentation and poor flavor during acidic fruit wine production. In the present study, we estab...

journal_title：Applied microbiology and biotechnology

authors： Tian T,Wu D,Ng CT,Yang H,Sun J,Liu J,Lu J

更新日期：2020-04-01 00:00:00

abstract：:A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

journal_title：Applied microbiology and biotechnology

authors： Melin ES,Ferguson JF,Puhakka JA

更新日期：1997-06-01 00:00:00