Abstract：
:Acid tolerance and aroma profile are crucial factors for wine production in Saccharomyces cerevisiae. However, most wine yeasts to date fail to endure low-pH environments, therefore resulting in problems such as lengthened fermentation and poor flavor during acidic fruit wine production. In the present study, we established a multiple-step screening strategy, which was composed of atmospheric and room temperature plasma (ARTP), high-throughput screening (HTS), and laboratory adaptive evolution (ALE), to screen yeast strains for potential wine-producing with enhanced performances during low pH conditions. Importantly, we obtained the S. cerevisiae strain from the mutant library, ET008-c54, which displayed exhibited excellent performances in survival rate, fermentation time, aroma profile, and genetic stability. More specifically, the survival rate of ET008-c54 at low pH was increased by 10-fold, the fermentation time of greengage plum wine was shortened by about 70%, and the content of main aroma compounds was significantly increased by 52%. Collectively, we demonstrate the practical application of the screening platform designed for discovering mutant strains in winemaking technology.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Tian T,Wu D,Ng CT,Yang H,Sun J,Liu J,Lu Jdoi
10.1007/s00253-020-10451-zsubject
Has Abstractpub_date
2020-04-01 00:00:00pages
3097-3107issue
7eissn
0175-7598issn
1432-0614pii
10.1007/s00253-020-10451-zjournal_volume
104pub_type
杂志文章abstract：:Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production and the presence of host cell proteases is related to: (1) Isopropyl-beta- D-thiogalactopyranoside (IPTG) induction, (2) cell-mass concen...
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abstract：:Significant resources are invested into efforts to improve the production yields of natural products from Actinobacteria, a well-recognized source of leads for several industries, most notably pharmaceutical one. Introduction of changes into genes for ribosomal protein S12 (rpsL) and/or 16S rRNA methylation (rsmG) is ...
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更新日期：2019-09-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2015-03-01 00:00:00
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doi：10.1007/s00253-017-8259-8
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abstract：:Maltol derivatives are utilized in a variety of fields due to their metal-chelating abilities, and modification of the 2-methyl side chain is known to effectively expand their functional diversity. In the present study, microbial enzymes were screened for hydroxylating activity towards the 2-methyl group in a maltol d...
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更新日期：2017-09-01 00:00:00
abstract：:The published online version contains editing mistake in Table 2. See below for the corrected Table. ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,已发布勘误
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doi：10.1007/s00253-020-10781-y
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-006-0353-2
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abstract：:Evolvulus alsinoides L. is used for preparation of 'Shankhapushpi', an important popular ayurvedic drug that contributes considerably to the improvement of memory power. The improvement is attributed to the presence of furanocoumarin scopoletin, a metabolite with a wide range of biological activities. This report desc...
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更新日期：2016-04-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2015-10-01 00:00:00
abstract：:In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...
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doi：10.1007/s00253-011-3106-9
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530051041
更新日期：1997-08-01 00:00:00
abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...
journal_title：Applied microbiology and biotechnology
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更新日期：2007-08-01 00:00:00
abstract：:After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast, repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applying RB cultivation, b...
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pub_type： 杂志文章,评审
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更新日期：2018-06-01 00:00:00
abstract：:An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temper...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-002-1137-y
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-019-09661-x
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