Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase.

abstract：:This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

journal_title：Applied microbiology and biotechnology

authors： Albadran HA,Monteagudo-Mera A,Khutoryanskiy VV,Charalampopoulos D

更新日期：2020-07-01 00:00:00

abstract：:We report on a pheromone-based inter-species communication system, allowing for a controlled cell-cell communication between the two species Saccharomyces cerevisiae and Schizosaccharomyces pombe as a proof of principle. It exploits the mating response pathways of the two yeast species employing the pheromones, α- or ...

journal_title：Applied microbiology and biotechnology

authors： Hennig S,Clemens A,Rödel G,Ostermann K

更新日期：2015-02-01 00:00:00

abstract：:Many microorganisms, growing on aging flue-cured tobacco leaves, play a part in its fermentation process. These microflora were identified and described by culture-dependent methods earlier. In this study we report the identity of the microflora growing on the tobacco leaf surface by employing culture-independent meth...

journal_title：Applied microbiology and biotechnology

authors： Zhao M,Wang B,Li F,Qiu L,Li F,Wang S,Cui J

更新日期：2007-01-01 00:00:00

abstract：:Globotriose (Galα1-4Galβ1-4Glc) is an important cell surface epitope that acts as the receptor for Shiga-like toxins, and it is also the core structure of Globo H and SSEA4 that are tumor-associated glycans. Hence, the enzymatic synthesis of globotriose would be necessary for the development of carbohydrate-based ther...

journal_title：Applied microbiology and biotechnology

authors： Gong W,Xu L,Gu G,Lu L,Xiao M

更新日期：2016-08-01 00:00:00

abstract：:Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Zhou Y,Yi T,Zhao M,Xie N,Lei M,Liu Q,Shao Y,Chen F

更新日期：2016-08-01 00:00:00

abstract：:Removal of tetrachloroethylene (perchloroethylene; C2Cl4) by microbial consortia from two sites with different C2Cl4 exposure histories was examined in a bench-scale anaerobic column bioreactor. It was hypothesized that optimal removal would be observed in the reactor packed with sediments having an extensive exposure...

journal_title：Applied microbiology and biotechnology

authors： Noftsker C,Watwood ME

更新日期：1997-09-01 00:00:00

abstract：:Corynebacterium glutamicum CCTCC M201005 produces a novel polygalacturonic acid bioflocculant, REA-11, consisting of galacturonic acid as the main structural unit. A biosynthetic pathway of REA-11 in C. glutamicum CCTCC M201005 was proposed. Evidence for the biosynthetic pathway was provided by: (1) analyzing the resp...

journal_title：Applied microbiology and biotechnology

authors： Li Y,He N,Guan H,Du G,Chen J

更新日期：2003-12-01 00:00:00

abstract：:Human interleukin 6 (hIL-6) overproduced in Escherichia coli HB101 was found to partially retain the initiator methionine (Met) residue (Met-hIL-6). In order to remove the residual N-terminal Met in vivo, an attempt was made to express hIL-6 in aminopeptidase-P (Ap-P)-hyperproducing strains, since the N-terminus Met-P...

journal_title：Applied microbiology and biotechnology

authors： Yasueda H,Kikuchi Y,Kojima H,Nagase K

更新日期：1991-11-01 00:00:00

abstract：:A novel beta-glucosidase (BGL)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal BGL activity of 12.3...

journal_title：Applied microbiology and biotechnology

authors： Jeya M,Joo AR,Lee KM,Tiwari MK,Lee KM,Kim SH,Lee JK

更新日期：2010-05-01 00:00:00

abstract：:A comprehensive understanding of genotype-phenotype links in bacteria is the primary theme of bacterial functional genomics. Transposon sequencing (Tn-seq) or its equivalent methods that combine random transposon mutagenesis and next-generation sequencing (NGS) represent a powerful approach to understand gene function...

journal_title：Applied microbiology and biotechnology

authors： Kwon YM,Ricke SC,Mandal RK

更新日期：2016-01-01 00:00:00

abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

journal_title：Applied microbiology and biotechnology

authors： Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

更新日期：2020-10-01 00:00:00

abstract：:Freezing lactic acid bacteria often leads to cell death and loss of technological properties. Our objective was to provide an in-depth characterization of the biophysical properties of the Lactobacillus delbrueckii subsp. bulgaricus membrane in relation to its freeze resistance. Freezing was represented as a combinati...

journal_title：Applied microbiology and biotechnology

authors： Meneghel J,Passot S,Dupont S,Fonseca F

更新日期：2017-02-01 00:00:00

abstract：:Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escheric...

journal_title：Applied microbiology and biotechnology

authors： Carlsen S,Ajikumar PK,Formenti LR,Zhou K,Phon TH,Nielsen ML,Lantz AE,Kielland-Brandt MC,Stephanopoulos G

更新日期：2013-07-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:We describe a novel chromosome engineering technique for shuffling selected regions of chromosomes from two strains in Saccharomyces cerevisiae: The technique starts with the construction of MATa and MATalpha strains in which a particular chromosome is split at exactly the same site in both strains such that the split...

journal_title：Applied microbiology and biotechnology

authors： Sugiyama M,Yamamoto E,Mukai Y,Kaneko Y,Nishizawa M,Harashima S

更新日期：2006-10-01 00:00:00

abstract：:The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

更新日期：2012-11-01 00:00:00

abstract：:Pneumococcal surface protein A (PspA) is essential for Streptococcus pneumoniae virulence and its use either as a novel pneumococcal vaccine or as carrier in a conjugate vaccine would improve the protection and the coverage of the vaccine. Within this context, the development of scalable production and purification pr...

journal_title：Applied microbiology and biotechnology

authors： Carvalho RJ,Cabrera-Crespo J,Tanizaki MM,Gonçalves VM

更新日期：2012-05-01 00:00:00

abstract：:Bacterial cells are protected by an exoskeleton, the stabilizing and shape-maintaining cell wall, consisting of the complex macromolecule peptidoglycan. In view of its function, it could be assumed that the cell wall is a static structure. In truth, however, it is steadily broken down by peptidoglycan-cleaving enzymes...

journal_title：Applied microbiology and biotechnology

authors： Reith J,Mayer C

更新日期：2011-10-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00

abstract：:In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of β-lactam antibiotics. Kinetically controlled enzymatic syntheses of cephalosporins of novel generations in which PGA catalyzes coupling of activated acyl donor...

journal_title：Applied microbiology and biotechnology

authors： Marešová H,Plačková M,Grulich M,Kyslík P

更新日期：2014-04-01 00:00:00

abstract：:Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken a...

journal_title：Applied microbiology and biotechnology

authors： Kacena MA,Merrell GA,Manfredi B,Smith EE,Klaus DM,Todd P

更新日期：1999-02-01 00:00:00

abstract：:Plant-parasitic nematodes are estimated to cause global annual losses of more than US$ 100 billion. The number of registered nematicides has declined substantially over the last 25 years due to concerns about their non-specific mechanisms of action and hence their potential toxicity and likelihood to cause environment...

journal_title：Applied microbiology and biotechnology

authors： Degenkolb T,Vilcinskas A

更新日期：2016-05-01 00:00:00

abstract：:Escherichia coli KJ122 was previously engineered to produce high concentration and yield of succinate in mineral salt medium containing glucose and sucrose under anaerobic conditions. However, this strain does not efficiently utilize xylose. To improve the xylose uptake and utilization in the strain KJ122, xylFGH and ...

journal_title：Applied microbiology and biotechnology

authors： Khunnonkwao P,Jantama SS,Kanchanatawee S,Jantama K

更新日期：2018-01-01 00:00:00

abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...

journal_title：Applied microbiology and biotechnology

authors： Chang IA,Kim IH,Kang SC,Hou CT,Kim HR

更新日期：2007-02-01 00:00:00

abstract：:There are alarming reports of growing microbial resistance to all classes of antimicrobial agents used against different infections. Also the existing classes of anticancer drugs used against different tumours warrant the urgent search for more effective alternative agents for treatment. Broad-spectrum bioactivities o...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Kon K,Ingle A,Duran N,Galdiero S,Galdiero M

更新日期：2014-03-01 00:00:00

abstract：:The objectives of this work were to demonstrate the potential of a two-phase sequencing batch reactor in degrading xenobiotics and to evaluate the kinetic parameters leading to a mathematical model of the system. 4-Nitrophenol (4NP), a typical representative of substituted phenols, was selected as the target xenobioti...

journal_title：Applied microbiology and biotechnology

authors： Tomei MC,Annesini MC,Rita S,Daugulis AJ

更新日期：2008-10-01 00:00:00

abstract：:This study investigated the characterization and flocculation mechanism of a bioflocculant prepared using potato starch wastewater. The optimal culture conditions of this strain were determined as 4 g K2HPO4, 2 g KH2PO4, 0.2 g MgSO4, 0.1 g NaCl, and 2.0 g urea dissolved in 1.0 L potato starch wastewater with no need o...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Lau AK,Zhang Y,Zhao J

更新日期：2015-07-01 00:00:00

abstract：:Production of a novel antimicrobial tropolone, beta-thujaplicin, in Cupressus lusitanica suspension cultures was studied by using a variety of chemicals and fungal elicitors. Sodium alginate, chitin, and methyl jasmonate resulted in 2-, 2.5-, and 3-fold higher beta-thujaplicin production, respectively, than in the con...

journal_title：Applied microbiology and biotechnology

authors： Zhao J,Fujita K,Yamada J,Sakai K

更新日期：2001-04-01 00:00:00

abstract：:Arabinofuranose substitutions on xylan are known to interfere with enzymatic hydrolysis of this primary hemicellulose. In this work, two novel α-L-arabinofuranosidases (ABFs), TtABF51A from Thielavia terrestris and EpABF62C from Eupenicillium parvum, were characterized and functionally analyzed. From sequences analyse...

journal_title：Applied microbiology and biotechnology

authors： Long L,Sun L,Lin Q,Ding S,St John FJ

更新日期：2020-10-01 00:00:00

abstract：:Promoters of sorbose dehydrogenase gene sdh and sorbosone dehydrogenase gene sndh (Psdh and Psndh) in Ketogulonicigenium vulgare DSM 4025 were identified. The transcription initiation site (TIS) of Psdh was guanine 74 bp upstream of the start codon of sdh and the TIS of Psndh was adenine 113 bp upstream of the first c...

journal_title：Applied microbiology and biotechnology

authors： Fu S,Zhang W,Guo A,Wang J

更新日期：2007-07-01 00:00:00