Molecular cloning and characterization of a GH11 endoxylanase from Chaetomium globosum, and its use in enzymatic pretreatment of biomass.

abstract：:Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h(-1) but accumulated almost no poly-3-hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (mu = 0....

journal_title：Applied microbiology and biotechnology

authors： Sun Z,Ramsay JA,Guay M,Ramsay BA

更新日期：2007-02-01 00:00:00

abstract：:Inhibitory compounds liberated from lignocellulose pretreatment are representative toxic chemicals that repress microbial growth and metabolism. A tolerant strain of the industrial yeast Saccharomyces cerevisiae is able to detoxify a major class of toxic compounds while producing ethanol. Knowledge on the yeast tolera...

journal_title：Applied microbiology and biotechnology

authors： Liu ZL,Huang X,Zhou Q,Xu J

更新日期：2019-07-01 00:00:00

abstract：:Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as beta-galactosidase activity. Transcription of the crystal protein gene fusion began im...

journal_title：Applied microbiology and biotechnology

authors： Ahmed HK,Mitchell WJ,Priest FG

更新日期：1995-05-01 00:00:00

abstract：:Sphingobium sp. strain TCM1 can significantly degrade chlorinated organophosphorus flame retardants, such as tris(2-chloroethyl) phosphate. The PhoK of strain TCM1 (Sb-PhoK) is the main alkaline phosphatase (APase) that catalyzes the last step in the degradation pathway. Here, we purified and characterized Sb-PhoK pro...

journal_title：Applied microbiology and biotechnology

authors： Takahashi S,Morooka Y,Kumakura T,Abe K,Kera Y

更新日期：2020-02-01 00:00:00

abstract：:Only two polyethylene glycol terephthalate (PET)-degrading enzymes have been reported, and their mechanism for the biochemical degradation of PET remains unclear. To identify a novel PET-degrading enzyme, a putative cutinase gene (cut190) was cloned from the thermophile Saccharomonospora viridis AHK190 and expressed i...

journal_title：Applied microbiology and biotechnology

authors： Kawai F,Oda M,Tamashiro T,Waku T,Tanaka N,Yamamoto M,Mizushima H,Miyakawa T,Tanokura M

更新日期：2014-12-01 00:00:00

abstract：:A reactive iron barrier in a contaminated aquifer with low pH was found to dechlorinate 1,2-dichloroethane (1,2-DCA) in situ. This chlorinated ethane is known to resist abiotic reduction by zero valent iron. Samples taken up-gradient and within the barrier were used to inoculate anaerobic batch cultures amended with v...

journal_title：Applied microbiology and biotechnology

authors： Zemb O,Lee M,Low A,Manefield M

更新日期：2010-09-01 00:00:00

abstract：:Cyanobacterial blooms have become a serious problem in Lake Taihu during the last 20 years, and Microcystis aeruginosa and Synechococcus sp. are the two dominant species in cyanobacterial blooms of Lake Taihu. A freshwater bacterial strain, Shewanella sp. Lzh-2, with strong algicidal properties against harmful cyanoba...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Lin S,Liu X,Tan J,Pan J,Yang H

更新日期：2014-05-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:The suitability of culture supernatant from Streptomyces albus ATCC 3005 for use in the biobleaching of eucalyptus kraft pulp was investigated. S. albus was found to grow on a minimal salts medium containing oat spelts xylan and yeast extract as the main carbon and nitrogen sources, respectively. Maximal extracellular...

journal_title：Applied microbiology and biotechnology

authors： Antonopoulos VT,Hernandez M,Arias ME,Mavrakos E,Ball AS

更新日期：2001-10-01 00:00:00

abstract：:Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-E...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Guo C,Zhou L,Zhang Z,Huang Y,Yang J,Bai X,Yang K

更新日期：2015-09-01 00:00:00

abstract：:Elimination of acetate overflow in aerobic cultivation of Escherichia coli would improve many bioprocesses as acetate accumulation in the growth environment leads to numerous negative effects, e.g. loss of carbon, inhibition of growth, target product synthesis, etc. Despite many years of studies, the mechanism and reg...

journal_title：Applied microbiology and biotechnology

authors： Peebo K,Valgepea K,Nahku R,Riis G,Oun M,Adamberg K,Vilu R

更新日期：2014-06-01 00:00:00

abstract：:The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon reso...

journal_title：Applied microbiology and biotechnology

authors： Lévêque C,Ferracci G,Maulet Y,Grand-Masson C,Seagar M,El Far O

更新日期：2015-05-01 00:00:00

abstract：:Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

journal_title：Applied microbiology and biotechnology

authors： Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

更新日期：2017-11-01 00:00:00

abstract：:The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic ni...

journal_title：Applied microbiology and biotechnology

authors： Saha BC

更新日期：2006-10-01 00:00:00

abstract：:Aliphatic ketones, such as 2-butanone and 3-hexanone, with only one carbon difference among side chains adjacent to the carbonyl carbon are difficult to be reduced enantioselectively. In this study, we utilized an acetophenone reductase from Geotrichum candidum NBRC 4597 (GcAPRD) to reduce challenging aliphatic ketone...

journal_title：Applied microbiology and biotechnology

authors： Koesoema AA,Sugiyama Y,Xu Z,Standley DM,Senda M,Senda T,Matsuda T

更新日期：2019-12-01 00:00:00

abstract：:A microbial peptide amidase was found in a limited screening and purified about 500-fold from Stenotrophomonas maltophilia. The native enzyme has a molecular mass of 38 kDa (gel filtration). The sequence of the first 16 amino acids was determined by Edman degradation. The isoelectric point was found to be around 5.8. ...

journal_title：Applied microbiology and biotechnology

authors： Stelkes-Ritter U,Wyzgol K,Kula MR

更新日期：1995-12-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:Myxobacteria are Gram-negative soil microorganisms that prey on other microorganisms. Myxobacteria have significant potential for applications in biotechnology because of their extraordinary ability to produce natural products such as secondary metabolites. Myxobacteria also stand out as model organisms for the study ...

journal_title：Applied microbiology and biotechnology

authors： Ueki T,Inouye S

更新日期：2006-08-01 00:00:00

abstract：:By using immobilized baker's yeast repeatedly in isooctane with occasional reactivation by cultivation, we succeeded in the preparation of an organic solvent-tolerant strain, named KK21, which could grow in the presence of isooctane. This is the first report on an organic solvent-tolerant strain from baker's yeast. St...

journal_title：Applied microbiology and biotechnology

authors： Kawamoto T,Kanda T,Tanaka A

更新日期：2001-05-01 00:00:00

abstract：:The genus Aspergillus is widely used for the production of plant cell wall polysaccharide-degrading enzymes. The range of enzymes purified from these fungi covers nearly every function required for the complete degradation of cellulose, xyloglucan, xylan, galacto(gluco)mannan and pectin. This paper describes the Asper...

journal_title：Applied microbiology and biotechnology

authors： de Vries RP

更新日期：2003-03-01 00:00:00

abstract：:β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the...

journal_title：Applied microbiology and biotechnology

authors： Parhiz H,Ketcham SA,Zou G,Ghosh B,Fratz-Berilla EJ,Ashraf M,Ju T,Madhavarao CN

更新日期：2019-08-01 00:00:00

abstract：:Rhodococcus erythropolis U23A is a polychlorinated biphenyl (PCB)-degrading bacterium isolated from the rhizosphere of plants grown on a PCB-contaminated soil. Strain U23A bphA exhibited 99% identity with bphA1 of Rhodococcus globerulus P6. We grew Arabidopsis thaliana in a hydroponic axenic system, collected, and con...

journal_title：Applied microbiology and biotechnology

authors： Toussaint JP,Pham TT,Barriault D,Sylvestre M

更新日期：2012-09-01 00:00:00

abstract：:Two compost piles were prepared, using two ventilation systems: forced ventilation and ventilation through mechanical turning. The material to compost was a mixture of orange waste, olive pomace, and grass clippings (2:1:1 v/v). During the composting period (375 days), samples were periodically taken from both piles, ...

journal_title：Applied microbiology and biotechnology

authors： Coelho L,Reis M,Dionísio L

更新日期：2013-05-01 00:00:00

abstract：:To reveal the succession procedure of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) community structure in sequencing biofilm batch reactor (SBBR), the molecular biological techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and real-time PCR were applied. DGGE showed that th...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Zhang C,Rong H

更新日期：2014-05-01 00:00:00

abstract：:The sequence of gene xynB encoding xylanase B from Paenibacillus sp. BP-23 was determined. It revealed an open reading frame of 999 nucleotides encoding a protein of 38,561 Da. The deduced amino acid sequence of xylanase B shows that the N-terminal region of the enzyme lacks the features of a signal peptide. When the ...

journal_title：Applied microbiology and biotechnology

authors： Gallardo O,Diaz P,Pastor FI

更新日期：2003-05-01 00:00:00

abstract：:For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement...

journal_title：Applied microbiology and biotechnology

authors： Sindelar G,Wendisch VF

更新日期：2007-09-01 00:00:00

abstract：:Stereoselective carbon-carbon bond formation with aldolases has become an indispensable tool in preparative synthetic chemistry. In particular, the dihydroxyacetone phosphate (DHAP)-dependent aldolases are attractive because four different types are available that allow access to a complete set of diastereomers of vic...

journal_title：Applied microbiology and biotechnology

authors： Schümperli M,Pellaux R,Panke S

更新日期：2007-05-01 00:00:00

abstract：:Microbial genome sequencing has left a legacy of annotated yet uncharacterized genes or open reading frames, activities that may have useful applications in health and/or the environment. We are interested in the discovery and characterization of potentially new pectinolytic activities for the enzymatic retting of nat...

journal_title：Applied microbiology and biotechnology

authors： Xiao Z,Boyd J,Grosse S,Beauchemin M,Coupe E,Lau PC

更新日期：2008-04-01 00:00:00

abstract：:The Bacillus subtilis lipoprotein PrsA enhances the yield of several homologous and heterologous exported proteins in B. subtilis by being involved in the posttranslocational stage of the secretion process. In this work, we have studied the effect of B. subtilis PrsA on the secretion of Bacillus amyloliquefaciens alph...

journal_title：Applied microbiology and biotechnology

authors： Lindholm A,Ellmén U,Tolonen-Martikainen M,Palva A

更新日期：2006-12-01 00:00:00

abstract：:An enzyme preparation from the thermophilic fungus Humicola insolens, Ultraflo L, was able to solubilise more than half of the biomass of brewer's grain and wheat bran, two agro-industrial co-products. While almost all of the ferulic acid was released in the free form, the majority of diferulates were released still a...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

更新日期：2004-06-01 00:00:00