Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy.

abstract：:An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). Th...

journal_title：Applied microbiology and biotechnology

authors： Pfruender H,Amidjojo M,Hang F,Weuster-Botz D

更新日期：2005-06-01 00:00:00

abstract：:Sialylation affects circulating half-life, charge distribution, and other biochemical properties of therapeutic glycoproteins. Loss of protein sialylation during glycoprotein-producing bioprocesses could lead to a low final protein sialylation level and bring negative effects on subsequent clinical efficacy. In this w...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Liu X,Xiao Z,Liu J,Zhao L,Tan WS,Fan L

更新日期：2019-06-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:The successful conversion of plant production systems from conventional resource-exhausting to sustainable strategies depends on knowledge-based management of environmental factors. Root-inhabiting fungi came more and more into focus because their hyphae connect in ideal manner resources and challenges of the surround...

journal_title：Applied microbiology and biotechnology

authors： Franken P

更新日期：2012-12-01 00:00:00

abstract：:A customer- and environment-friendly method for the decolorization azo dyes was developed. Azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. A new reduced nicotinamide adenine dinucleotide-dependent azoreductase of Bacillus cereus, which showed high potent...

journal_title：Applied microbiology and biotechnology

authors： Pricelius S,Held C,Murkovic M,Bozic M,Kokol V,Cavaco-Paulo A,Guebitz GM

更新日期：2007-11-01 00:00:00

abstract：:Antibiotic resistance is a global challenge and represents a growing threat on human health worldwide. Wastewater treatment plants (WWTPs) are generally considered as hotspots for control and/or dissemination of antibiotic resistance. The role of antibiotics, antibacterial residues, and heavy metals played on the evol...

journal_title：Applied microbiology and biotechnology

authors： Gao P,He S,Huang S,Li K,Liu Z,Xue G,Sun W

更新日期：2015-05-01 00:00:00

abstract：:Biofilms are widely used in wastewater treatment for their particular enhancement of nitrogen removal and other significant advantages. In this study, the diversity and potential functions of nitrogen removal bacteria in suspended activated sludge (AS) and biofilm of a full-scale hybrid reactor were uncovered by metag...

journal_title：Applied microbiology and biotechnology

authors： Chao Y,Mao Y,Yu K,Zhang T

更新日期：2016-09-01 00:00:00

abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

journal_title：Applied microbiology and biotechnology

authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

更新日期：2002-10-01 00:00:00

abstract：:Physico-chemical parameters, hydrological conditions, and microbial interactions can affect the growth and persistence of cyanobacteria, but the interacting effects among these bloom-forming factors are still poorly known. This hampers our capacity to predict the occurrence of cyanobacterial bloom accurately. Here, we...

journal_title：Applied microbiology and biotechnology

authors： Song H,Xu J,Lavoie M,Fan X,Liu G,Sun L,Fu Z,Qian H

更新日期：2017-02-01 00:00:00

abstract：:Recent work has shed light on the abundance and diversity of D-amino acids in bacterial extracellular/periplasmic molecules, bacterial cell culture, and bacteria-rich environments. Within the extracellular/periplasmic space, D-amino acids are necessary components of peptidoglycan, and disruption of their synthesis lea...

journal_title：Applied microbiology and biotechnology

authors： Radkov AD,Moe LA

更新日期：2014-06-01 00:00:00

abstract：:Ionic liquid (IL) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. More in-depth research involving environment-friendly IL is much needed to explore pretreatment green route. In our case, IL 1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) was chose...

journal_title：Applied microbiology and biotechnology

authors： Li Q,Jiang X,He Y,Li L,Xian M,Yang J

更新日期：2010-06-01 00:00:00

abstract：:The gene encoding a putative triphenylmethane reductase (TMR)-like protein derived from Geobacillus thermoglucosidasius C56-Y593 (named as GtAZR) was synthesized, heterologously expressed in Escherichia coli, and extensively characterized for the first time. The recombinant GtAZR displayed its maximum activity at pH 5...

journal_title：Applied microbiology and biotechnology

authors： Gao F,Ding H,Shao L,Xu X,Zhao Y

更新日期：2015-01-01 00:00:00

abstract：:Porcine parvovirus type 1 (PPV1) is a major causative agent of embryonic and fetal death in swine. The PPV1 VP2 protein is closely associated with viral immunogenicity for eliciting neutralizing antibodies, but its antigenic structures have been largely unknown. We generated three monoclonal antibodies (MAbs) against ...

journal_title：Applied microbiology and biotechnology

authors： Sun J,Huang L,Wei Y,Wang Y,Chen D,Du W,Wu H,Feng L,Liu C

更新日期：2015-11-01 00:00:00

abstract：:Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S...

journal_title：Applied microbiology and biotechnology

authors： Bräu B,Hilgenfeld R,Schlingmann M,Marquardt R,Birr E,Wohlleben W,Aufderheide K,Pühler A

更新日期：1991-01-01 00:00:00

abstract：:The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the ...

journal_title：Applied microbiology and biotechnology

authors： Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:The primary explosive found in most land mines, 2,4,6-trinitrotoluene (2,4,6-TNT), is often accompanied by 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB) impurities. The latter two compounds, being more volatile, have been reported to slowly leak through land mine covers and permeate the soil under whic...

journal_title：Applied microbiology and biotechnology

authors： Yagur-Kroll S,Lalush C,Rosen R,Bachar N,Moskovitz Y,Belkin S

更新日期：2014-01-01 00:00:00

abstract：:L-tryptophan (L-trp) is a biosynthetic precursor of various bioactive components with pharmaceutical interest. The development of an efficient L-trp production strain using targeted molecular engineering approaches is challenging due to the requirement of several precursors and the complex regulations of the pathways ...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Zeng AP

更新日期：2017-01-01 00:00:00

abstract：:Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, produced a variety of monohydroxy androstene derivatives and an oxidized product when incubated with exogenous testosterone for 24 h at 65 degrees C. The major metabolite was identified as androst-4-en-3,17-dione while minor metabo...

journal_title：Applied microbiology and biotechnology

authors： Al-Awadi S,Afzal M,Oommen S

更新日期：2003-07-01 00:00:00

abstract：:Caffeic acid derivatives (CADs) are a group of bioactive compounds which are produced in Echinacea species especially Echinacea purpurea, Echinacea angustifolia, and Echinacea pallida. Echinacea is a popular herbal medicine used in the treatment of common cold and it is also a prominent dietary supplement used through...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Kim YS,Park SY,Paek KY

更新日期：2014-09-01 00:00:00

abstract：:Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from ...

journal_title：Applied microbiology and biotechnology

authors： Leite HA,Silva AB,Gomes FP,Gramacho KP,Faria JC,de Souza JT,Loguercio LL

更新日期：2013-03-01 00:00:00

abstract：:Shewanella decolorationis S12, a representative dissimilatory azo-reducing bacterium of Shewanella genus, can grow by coupling the oxidation of hydrogen to the reduction of azo compounds as the sole electron acceptor, indicating that an uptake hydrogenase is an important component for electron transfer for azoreductio...

journal_title：Applied microbiology and biotechnology

authors： Hong YG,Guo J,Sun GP

更新日期：2008-09-01 00:00:00

abstract：:Polyphosphosphate kinases (PPKs) catalyse the reversible transfer of the γ-phosphate group of a nucleoside-triphosphate to a growing chain of polyphosphate. Most known PPKs are specific for ATP, but some can also use GTP as a phosphate donor. In this study, we describe the properties of a PPK2-type PPK of the β-proteo...

journal_title：Applied microbiology and biotechnology

authors： Hildenbrand JC,Teleki A,Jendrossek D

更新日期：2020-08-01 00:00:00

abstract：:Exposure of Synechococcus leopoliensis to selenite in the light resulted in orange-colored granules associated with the cells. No such particles were made in dark grown cells or when selenite was replaced by selenate. Light and scanning electron microscopy revealed that the particles formed inside the cells. Furthermo...

journal_title：Applied microbiology and biotechnology

authors： Hnain A,Brooks J,Lefebvre DD

更新日期：2013-12-01 00:00:00

abstract：:C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after ab...

journal_title：Applied microbiology and biotechnology

authors： Zhu Y,Chen XB,Wang KB,Li YX,Bai KZ,Kuang TY,Ji HB

更新日期：2007-02-01 00:00:00

abstract：:Thiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial de...

journal_title：Applied microbiology and biotechnology

authors： Perruchon C,Chatzinotas A,Omirou M,Vasileiadis S,Menkissoglou-Spiroudi U,Karpouzas DG

更新日期：2017-05-01 00:00:00

abstract：:The sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium Meiothermus ruber H328 was presented with extensive data. Aerobic cultivation with moderately thermophilic strain H328 at 55 degrees C for 6 days led to the apparently complete decay of the truly intact feat...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Yamada Y,Mitsuya H,Shigeri Y,Yoshida Y,Saito Y,Matsui H,Watanabe K

更新日期：2009-04-01 00:00:00

abstract：:A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M....

journal_title：Applied microbiology and biotechnology

authors： Li Z,Geng M,Yang H

更新日期：2015-01-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii is a well-documented pathogen associated with hospital-acquired infections. In addition to multidrug resistance, A. baumannii can also become resistant to colistin, the antibiotic treatment of last resort, by the loss of the lipopolysaccharide from its outer membrane. Here, ...

journal_title：Applied microbiology and biotechnology

authors： Tseng CC,Tsai YH,Hu A,Liou JW,Chang KC,Chang HH

更新日期：2016-10-01 00:00:00

abstract：:This study was undertaken to assess the antifungal performance of three different Lactobacillus species.Experiments were conducted in vitro and in situ to extend the shelf life of wheat bread. Standard sourdough analyses were performed characterising acidity and carbohydrate levels. Overall, the strains showed good in...

journal_title：Applied microbiology and biotechnology

authors： Axel C,Brosnan B,Zannini E,Peyer LC,Furey A,Coffey A,Arendt EK

更新日期：2016-02-01 00:00:00

abstract：:Microbial natural products, in particular, the ones produced by the members of the order Actinomycetales, will continue to represent an important route to the discovery of novel classes of bioactive compounds. As a result, the search for and discovery of lesser-known and/or novel actinomycetes is of significant intere...

journal_title：Applied microbiology and biotechnology

authors： Kurtböke DI

更新日期：2012-03-01 00:00:00