Development of a short-term assay based on the evaluation of the plasma membrane integrity of the alga Pseudokirchneriella subcapitata.

Abstract:

:Membrane integrity has been used as a criterion for the definition of cell viability. In the present work, staining conditions (time and dye concentration) for the evaluation of membrane integrity in a fluorescence microplate reader, using the membrane-impermeant nucleic-acid dye SYTOX Green, were optimized. Incubating Pseudokirchneriella subcapitata algal cells with 0.5 μmol/l SYTOX Green for 40 min allowed a clear discrimination between live (intact plasma membrane) and dead cells (with compromised plasma membrane). Algal cell suspensions, labelled with SYTOX Green, exhibited a green fluorescence proportional to the fraction of the cells with a permeabilized plasma membrane. The optimized staining conditions were used to assess the toxicity of 1-pentanol on P. subcapitata in a short-term exposure (6 h) assay. The loss of membrane integrity in the cell population increased with the concentration of 1-pentanol. The 6-h EC(10) and EC(50) values were 7,617 mg/l 1-pentanol (95 % confidence limits 4,670-9,327) and 12,818 mg/l 1-pentanol (95 % confidence limits 10,929-15,183), respectively. The developed microplate-based short-term assay can be useful in the high-throughput screening of toxics or environmental samples using the alga P. subcapitata.

authors

Machado MD,Soares EV

doi

10.1007/s00253-012-4185-y

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

1035-42

issue

4

eissn

0175-7598

issn

1432-0614

journal_volume

95

pub_type

杂志文章
  • Pentachlorophenol biodegradation kinetics of an oligotrophic fluidized-bed enrichment culture.

    abstract::A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530050994

    authors: Melin ES,Ferguson JF,Puhakka JA

    更新日期:1997-06-01 00:00:00

  • Protection of chickens against H9N2 avian influenza virus challenge with recombinant Lactobacillus plantarum expressing conserved antigens.

    abstract::Avian influenza virus (AIV) is spreading worldwide and is a serious threat to the health of poultry and humans. In many countries, low pathogenic AIVs, such as H9N2, have become an enormous economic burden on the commercial poultry industry because they cause mild respiratory disease and decrease egg production. A rec...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8230-8

    authors: Yang WT,Yang GL,Shi SH,Liu YY,Huang HB,Jiang YL,Wang JZ,Shi CW,Jing YB,Wang CF

    更新日期:2017-06-01 00:00:00

  • Degradation of atrazine by Frankia alni ACN14a: gene regulation, dealkylation, and dechlorination.

    abstract::Atrazine is transformed to N-isopropylammelide through hydroxyatrazine as an intermediate as indicated by high-performance liquid chromatography/mass spectroscopy in culture filtrates of Frankia alni ACN14a and Frankia sp. EuI1c. Both Frankia strains have the ability to degrade atrazine via dechlorination and dealkyla...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5665-z

    authors: Rehan M,Kluge M,Fränzle S,Kellner H,Ullrich R,Hofrichter M

    更新日期:2014-07-01 00:00:00

  • Recent advances on physiological functions and biotechnological production of epilactose.

    abstract::Epilactose (4-O-β-D-galactopyranosyl-D-mannose), an epimer of lactose, is a rare disaccharide existing extremely small quantities in heat-treated milk, in which epilactose is produced by non-enzymatic catalysis from lactose. This disaccharide is a kind of non-digestible carbohydrate, has a good prebiotic effect, and p...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-013-4687-2

    authors: Mu W,Li Q,Fan C,Zhou C,Jiang B

    更新日期:2013-03-01 00:00:00

  • Vertical migration of aggregated aerobic and anaerobic ammonium oxidizers enhances oxygen uptake in a stagnant water layer.

    abstract::Ammonium can be removed as dinitrogen gas by cooperating aerobic and anaerobic ammonium-oxidizing bacteria (AerAOB and AnAOB). The goal of this study was to verify putative mutual benefits for aggregated AerAOB and AnAOB in a stagnant freshwater environment. In an ammonium fed water column, the biological oxygen consu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-0944-6

    authors: Vlaeminck SE,Dierick K,Boon N,Verstraete W

    更新日期:2007-07-01 00:00:00

  • Novel approaches and achievements in biosynthesis of functional isoprenoids in Escherichia coli.

    abstract::Isoprenoids, also referred to as terpenes, are the most diverse class of natural products appearing in a variety of natural sources, specifically in higher plants, and have a wide range of biological functions. This review describes novel or recent approaches and achievements in pathway engineering of Escherichia coli...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-009-2166-6

    authors: Harada H,Misawa N

    更新日期:2009-10-01 00:00:00

  • Growth of Escherichia coli MG1655 on LB medium: monitoring utilization of amino acids, peptides, and nucleotides with transcriptional microarrays.

    abstract::Analysis of gene expression data related to assimilation and biosynthesis of nitrogen-containing compounds amino acids, peptides, and nucleotides was used to monitor availability of these nutrients to Escherichia coli MG1655 growing on Luria-Bertani medium. The data indicate that free amino acids and nucleotides only ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0310-5

    authors: Baev MV,Baev D,Radek AJ,Campbell JW

    更新日期:2006-07-01 00:00:00

  • The occurrence of killer, sensitive, and neutral yeasts in Brazilian Riesling Italico grape must and the effect of neutral strains on killing behaviour.

    abstract::The occurrence of killer toxins amongst yeast in Brazilian Riesling Italico grape must was investigated by using the sensitive strain EMBRAPA-26B as a reference strain at 18 degrees C and 28 degrees C. From a total of 85 previously isolated yeasts, 21 strains showed ability to kill the sensitive strain on unbuffered g...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530050791

    authors: da Silva GA

    更新日期:1996-09-01 00:00:00

  • Isolation and characterization of a novel cyanophycin synthetase from a deep-sea sediment metagenomic library.

    abstract::Cyanophycin is non-ribosomally synthesized protein-like copolymer. Synthesis of cyanophycin is catalyzed by cyanophycin synthetase (CphA). In this study, a novel cyanophycin synthetase CphA49 belonging to NOR5 clade of Gammaproteobacteria was identified with primer-based screening from a deep-sea sediment metagenomic ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4872-3

    authors: Du J,Li L,Ding X,Hu H,Lu Y,Zhou S

    更新日期:2013-10-01 00:00:00

  • Increased levels of antibiotic resistance in urban stream of Jiulongjiang River, China.

    abstract::The rapid global urbanization and other extensive anthropogenic activities exacerbated the worldwide human health risks induced by antibiotic resistance genes (ARGs). Knowledge of the origins and dissemination of ARGs is essential for understanding modern resistome, while little information is known regarding the over...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6416-5

    authors: Ouyang WY,Huang FY,Zhao Y,Li H,Su JQ

    更新日期:2015-07-01 00:00:00

  • Microbial biosurfactants for oil spill remediation: pitfalls and potentials.

    abstract::Spillage of fossil-based oils during their conveyance through water conduits are sporadic, but significant environmental disasters. As the viscous hydrocarbons of the crude oils spread on water surface and choke aquatic life to death, their effective degradation is crucial for ecological balance. Though chemical and m...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-9434-2

    authors: Patel S,Homaei A,Patil S,Daverey A

    更新日期:2019-01-01 00:00:00

  • Distribution of Oenococcus oeni populations in natural habitats.

    abstract::Oenococcus oeni is the lactic acid bacteria species most commonly encountered in wine, where it develops after the alcoholic fermentation and achieves the malolactic fermentation that is needed to improve the quality of most wines. O. oeni is abundant in the oenological environment as well as in apple cider and kombuc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-09689-z

    authors: Lorentzen MPG,Lucas PM

    更新日期:2019-04-01 00:00:00

  • An innovative tool reveals interaction mechanisms among yeast populations under oenological conditions.

    abstract::Alcoholic fermentation of grape must is a complex process, involving several yeast genera and species. The early stages in fermentation are dominated by non-Saccharomyces yeasts that are gradually replaced by the Saccharomyces cerevisiae species, which takes over the fermentation. Quantitative studies have reported th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4660-5

    authors: Renault PE,Albertin W,Bely M

    更新日期:2013-05-01 00:00:00

  • Expression and secretion of beta-glucuronidase and Pertussis toxin S1 by Streptomyces lividans.

    abstract::Streptomyces lividans IAF18, obtained by homologous cloning, is capable of over-producing XlnA. To investigate the possibility of the expression of foreign genes, various coding regions of the xylanase A gene (xlnA) were analysed. Expression/secretion vectors were constructed containing the regulatory elements of xlnA...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530050742

    authors: Paradis FW,Shareck F,Dupont C,Kluepfel D,Morosoli R

    更新日期:1996-06-01 00:00:00

  • Identification and quantification of Thiothrix eikelboomii using qPCR for early detection of bulking incidents in a full-scale water reclamation plant.

    abstract::This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6230-5

    authors: Asvapathanagul P,Olson BH,Gedalanga PB,Hashemi A,Huang Z,La J

    更新日期:2015-05-01 00:00:00

  • Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives.

    abstract::A yeast protein fragment complementation assay (PCA) system based on dihydrofolate reductase (DHFR) is difficult to be operated because it is not as sensitive to trimethoprim (TMP) as the system using a prokaryotic microorganism. Here, the PCA system using DHFR, specific inhibitors, and a substrate in the yeast Saccha...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1624-x

    authors: Shibasaki S,Sakata K,Ishii J,Kondo A,Ueda M

    更新日期:2008-09-01 00:00:00

  • Spatial and seasonal variation of methanogenic community in a river-bay system in South China.

    abstract::River-bay system is a transitional zone connecting land and ocean and an important natural source for methane emission. Methanogens play important roles in the global greenhouse gas budget and carbon cycle since they produce methane. The abundance and community assemblage of methanogens in such a dynamic system are no...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10613-z

    authors: Zhang CJ,Chen YL,Pan J,Wang YM,Li M

    更新日期:2020-05-01 00:00:00

  • Short-term effect of acetate and ethanol on methane formation in biogas sludge.

    abstract::Biochemical processes in biogas plants are still not fully understood. Especially, the identification of possible bottlenecks in the complex fermentation processes during biogas production might provide potential to increase the performance of biogas plants. To shed light on the question which group of organism consti...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5820-6

    authors: Refai S,Wassmann K,Deppenmeier U

    更新日期:2014-08-01 00:00:00

  • Enzymatic reduction of azo and indigoid compounds.

    abstract::A customer- and environment-friendly method for the decolorization azo dyes was developed. Azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. A new reduced nicotinamide adenine dinucleotide-dependent azoreductase of Bacillus cereus, which showed high potent...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1165-8

    authors: Pricelius S,Held C,Murkovic M,Bozic M,Kokol V,Cavaco-Paulo A,Guebitz GM

    更新日期:2007-11-01 00:00:00

  • Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and future trends.

    abstract::Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-006-0380-z

    authors: Vassilev N,Vassileva M,Nikolaeva I

    更新日期:2006-06-01 00:00:00

  • Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures.

    abstract::Overproduction of recombinant secretory proteins triggers numerous physiological perturbations. Depending on a given heterologous protein characteristics, the producer cell is faced with different challenges which lead to varying responses in terms of its physiology and the target protein production rate. In the prese...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10937-w

    authors: Korpys-Woźniak P,Kubiak P,Białas W,Celińska E

    更新日期:2020-11-01 00:00:00

  • Cellulose production and cellulose synthase gene detection in acetic acid bacteria.

    abstract::The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6198-1

    authors: Valera MJ,Torija MJ,Mas A,Mateo E

    更新日期:2015-02-01 00:00:00

  • Influence of the surface speciation on biofilm attachment to chalcopyrite by Acidithiobacillus thiooxidans.

    abstract::Surfaces of massive chalcopyrite (CuFeS2) electrodes were modified by applying variable oxidation potential pulses under growth media in order to induce the formation of different secondary phases (e.g., copper-rich polysulfides, S n(2-); elemental sulfur, S(0); and covellite, CuS). The evolution of reactivity (oxidat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4099-8

    authors: Lara RH,García-Meza JV,González I,Cruz R

    更新日期:2013-03-01 00:00:00

  • Trypsin promotes efficient influenza vaccine production in MDCK cells by interfering with the antiviral host response.

    abstract::Trypsin is commonly used in Madin-Darby canine kidney (MDCK) cell culture-based influenza vaccine production to facilitate virus infection by proteolytic activation of viral haemagglutinin, which enables multi-cycle replication. In this study, we were able to demonstrate that trypsin also interferes with pathogen defe...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3569-8

    authors: Seitz C,Isken B,Heynisch B,Rettkowski M,Frensing T,Reichl U

    更新日期:2012-01-01 00:00:00

  • A new mathematical model for nitrogen gas production with special emphasis on the role of attached growth media in anammox hybrid reactor.

    abstract::The present study emphasised on the development of new mathematical models based on mass balance and stoichiometry of nitrogen removal in anammox hybrid reactor (AHR). The performance of AHR at varying hydraulic retention times (HRTs) and nitrogen loading rates (NLRs) revealed that nitrogen removal efficiency (NRE) in...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6793-9

    authors: Tomar S,Gupta SK

    更新日期:2015-11-01 00:00:00

  • Batch and continuous cultivation of Anaerobiospirillum succiniciproducens for the production of succinic acid from whey.

    abstract::Batch and continuous cultivation of Anaerobiospirillum succiniciproducens were systematically studied for the production of succinic acid from whey. Addition of 2.5 g l(-1) yeast extract and 2.5 g l(-1) polypeptone per 10 g l(-1) whey was most effective for succinic acid production from both treated and nontreated whe...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530000331

    authors: Lee PC,Lee WG,Kwon S,Lee SY,Chang HN

    更新日期:2000-07-01 00:00:00

  • Utilization of protein-rich residues in biotechnological processes.

    abstract::A drawback of biotechnological processes, where microorganisms convert biomass constituents, such as starch, cellulose, hemicelluloses, lipids, and proteins, into wanted products, is the economic feasibility. Particularly the cost of nitrogen sources in biotechnological processes can make up a large fraction of total ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-015-7278-6

    authors: Pleissner D,Venus J

    更新日期:2016-03-01 00:00:00

  • Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol.

    abstract::After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast, repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applying RB cultivation, b...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051651

    authors: Arzumanov TE,Shishkanova NV,Finogenova TV

    更新日期:2000-05-01 00:00:00

  • Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034.

    abstract::Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central co...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8395-1

    authors: Ruiz Rodríguez LG,Aller K,Bru E,De Vuyst L,Hébert EM,Mozzi F

    更新日期:2017-08-01 00:00:00

  • High production of heterologous proteins in Escherichia coli using the thermo-regulated T7 expression system.

    abstract::The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-001-0891-6

    authors: Chao YP,Law W,Chen PT,Hung WB

    更新日期:2002-03-01 00:00:00