Evaluation and screening of efficient promoters to improve astaxanthin production in Xanthophyllomyces dendrorhous.

abstract：:The primary explosive found in most land mines, 2,4,6-trinitrotoluene (2,4,6-TNT), is often accompanied by 2,4-dinitrotoluene (2,4-DNT) and 1,3-dinitrobenzene (1,3-DNB) impurities. The latter two compounds, being more volatile, have been reported to slowly leak through land mine covers and permeate the soil under whic...

journal_title：Applied microbiology and biotechnology

authors： Yagur-Kroll S,Lalush C,Rosen R,Bachar N,Moskovitz Y,Belkin S

更新日期：2014-01-01 00:00:00

abstract：:Coastal sediments contaminated by polycyclic aromatic hydrocarbons (PAHs) can be candidates for remediation via an approach like land farming. Land farming converts naturally anaerobic sediments to aerobic environments, and the response of microbial communities, in terms of community structure alterations and correspo...

journal_title：Applied microbiology and biotechnology

authors： Obi CC,Adebusoye SA,Amund OO,Ugoji EO,Ilori MO,Hedman CJ,Hickey WJ

更新日期：2017-05-01 00:00:00

abstract：:For the fermentative production of plant-specific flavanones (naringenin, pinocembrin) by Escherichia coli, a plasmid was constructed which carried an artificial biosynthetic gene cluster, including PAL encoding a phenylalanine ammonia-lyase from a yeast, ScCCL encoding a cinnamate/coumarate:CoA ligase from the actino...

journal_title：Applied microbiology and biotechnology

authors： Miyahisa I,Kaneko M,Funa N,Kawasaki H,Kojima H,Ohnishi Y,Horinouchi S

更新日期：2005-09-01 00:00:00

abstract：:Purines are a primary source of carbon and nitrogen in soil; however, their metabolism is poorly understood in Streptomyces. Using a combination of proteomics, metabolomics, and metabolic engineering, we characterized the allantoin pathway in Streptomyces coelicolor. When cells grew in glucose minimal medium with alla...

journal_title：Applied microbiology and biotechnology

authors： Navone L,Casati P,Licona-Cassani C,Marcellin E,Nielsen LK,Rodriguez E,Gramajo H

更新日期：2014-01-01 00:00:00

abstract：:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

journal_title：Applied microbiology and biotechnology

authors： Kabel MA,Jurak E,Mäkelä MR,de Vries RP

更新日期：2017-06-01 00:00:00

abstract：:Biogranulation is a promising biotechnology developed for wastewater treatment. Biogranules exhibit a matrix microbial structure, and intensive research has shown that extracellular polymeric substances (EPS) are a major component of the biogranule matrix material in both anaerobic and aerobic granules. This paper aim...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Liu Y,Tay JH

更新日期：2004-08-01 00:00:00

abstract：:Aliphatic ketones, such as 2-butanone and 3-hexanone, with only one carbon difference among side chains adjacent to the carbonyl carbon are difficult to be reduced enantioselectively. In this study, we utilized an acetophenone reductase from Geotrichum candidum NBRC 4597 (GcAPRD) to reduce challenging aliphatic ketone...

journal_title：Applied microbiology and biotechnology

authors： Koesoema AA,Sugiyama Y,Xu Z,Standley DM,Senda M,Senda T,Matsuda T

更新日期：2019-12-01 00:00:00

abstract：:Drug resistance occurrence is a global healthcare concern responsible for the increased morbidity and mortality in hospitals, time of hospitalisation and huge financial loss. The failure of the most antibiotics to kill "superbugs" poses the urgent need to develop innovative strategies aimed at not only controlling bac...

journal_title：Applied microbiology and biotechnology

authors： Bassegoda A,Ivanova K,Ramon E,Tzanov T

更新日期：2018-03-01 00:00:00

abstract：:A study was conducted to optimize the biodegradation in soil slurries of phenanthrene initially dissolved in nonaqueous-phase liquids (NAPLs). The slow rate of degradation of phenanthrene in dibutyl phthalate was increased by addition of phenanthrene-degrading microorganisms to soil slurries containing the NAPL. The r...

journal_title：Applied microbiology and biotechnology

authors： Birman I,Alexander M

更新日期：1996-03-01 00:00:00

abstract：:The production of acetone, butanol and ethanol by fermentation of renewable biomass has potential to become a valuable industrial process. Mechanisms of solvent production and sporulation involve some common regulators in some ABE-producing clostridia, although details of the links between the pathways are not clear. ...

journal_title：Applied microbiology and biotechnology

authors： Kolek J,Diallo M,Vasylkivska M,Branska B,Sedlar K,López-Contreras AM,Patakova P

更新日期：2017-11-01 00:00:00

abstract：:Seed-based expression system is an attractive platform for the production of recombinant proteins in molecular farming. Despite the many advantages of molecular farming, little is known about the effect of the different subcellular accumulation of recombinant proteins on the endoplasmic reticulum (ER) quality control ...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Ma J,Sun D,Li H,Jiang C,Li X

更新日期：2015-08-01 00:00:00

abstract：:A gene encoding beta-glucanase activity from Bacillus amyloliquefaciens was subcloned in both orientations into plasmid shuttle vector pSA3. In only one orientation could a co-integrate be generated with the conjugative plasmid pVA797. The plasmid co-integrate was conjugated into Lactobacillus helveticus strain CNRZ45...

journal_title：Applied microbiology and biotechnology

authors： Thompson K,Collins M

更新日期：1991-06-01 00:00:00

abstract：:The manganese superoxide dismutase gene (sodM) is very highly expressed in Aspergillus oryzae. To elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (GUS) as a reporter. Deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodM promot...

journal_title：Applied microbiology and biotechnology

authors： Hisada H,Sano M,Ishida H,Hata Y,Abe Y,Machida M

更新日期：2006-10-01 00:00:00

abstract：:Seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus Neocallimastix frontalis. The fractions (ES1, ES3, ES2U1, ES2U2, ES2U4, ES2U3C1 and ES2U3C2) were separated from each other and from a fraction that could solubilize crystalline cellulose...

journal_title：Applied microbiology and biotechnology

authors： Wood TM,Wilson CA,McCrae SI

更新日期：1995-12-01 00:00:00

abstract：:Human immunodeficiency virus (HIV)/AIDS continues to spread worldwide, and most of the HIV-infected people living in developing countries have little or no access to highly active antiretroviral therapy. The development of efficient and low-cost microbicides to prevent sexual transmission of HIV should be given high p...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Fan J,Kitazato K

更新日期：2010-04-01 00:00:00

abstract：:Understanding of the effects of perturbation strategies on soil microorganisms is helpful in optimizing bioremediation systems and enhancing their efficiency. Four soil mesocosms were constructed for bioremediation of highly polycyclic aromatic hydrocarbon-contaminated soil using the flowering plant Orychophragmus vio...

journal_title：Applied microbiology and biotechnology

authors： Sun GD,Xu Y,Liu Y,Liu ZP

更新日期：2014-12-01 00:00:00

abstract：:Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central co...

journal_title：Applied microbiology and biotechnology

authors： Ruiz Rodríguez LG,Aller K,Bru E,De Vuyst L,Hébert EM,Mozzi F

更新日期：2017-08-01 00:00:00

abstract：:Transaminase enzymes (TAms) are becoming increasingly valuable in the chemist's toolbox as a biocatalytic route to chiral amines. Despite high profile successes, the lack of (R)-selective TAms and robustness under harsh industrial conditions continue to prove problematic. Herein, we report the isolation of the first h...

journal_title：Applied microbiology and biotechnology

authors： Kelly SA,Magill DJ,Megaw J,Skvortsov T,Allers T,McGrath JW,Allen CCR,Moody TS,Gilmore BF

更新日期：2019-07-01 00:00:00

abstract：:The use of antibiotics may accelerate the development of antibiotic resistance genes (ARGs) and bacteria which shade health risks to humans and animals. The emerging of ARGs in the water environment is becoming an increasing worldwide concern. Hundreds of various ARGs encoding resistance to a broad range of antibiotic...

journal_title：Applied microbiology and biotechnology

authors： Zhang XX,Zhang T,Fang HH

更新日期：2009-03-01 00:00:00

abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

journal_title：Applied microbiology and biotechnology

authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

更新日期：1996-08-01 00:00:00

abstract：:The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 d...

journal_title：Applied microbiology and biotechnology

authors： Corchero JL,Carbonell X,Benito A,Villaverde A

更新日期：1995-03-01 00:00:00

abstract：:For continuous production of the antibiotic nikkomycin immobilized cells have been used in a fluidized bed bioreactor. Cells of Streptomyces tendae were immobilized on sintered glass particles. Different biomass concentrations on the particles correspond to different thicknesses of mycelial layers because growth occur...

journal_title：Applied microbiology and biotechnology

authors： Trück HU,Chmiel H,Hammes WP,Trösch W

更新日期：1990-10-01 00:00:00

abstract：:Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes...

journal_title：Applied microbiology and biotechnology

authors： Chen CH,Tu CC,Kuo HY,Zeng RF,Yu CS,Lu HH,Liou ML

更新日期：2017-01-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:Confident protein-DNA interaction (PDI) data could significantly improve our understanding of transcriptional regulation network in both prokaryotes and eukaryotes. New methods need to be established for validating and mining the protein-DNA interaction data produced by bioinformatic tools and large-scale screening as...

journal_title：Applied microbiology and biotechnology

authors： Zeng J,Li Y,Zhang S,He ZG

更新日期：2012-02-01 00:00:00

abstract：:The active efflux system contributing to the solvent tolerance of Pseudomonas putida S12 was characterized physiologically. The mutant P. putida JK1, which lacks the active efflux system, was compared with the wild-type organism. None of 20 known substrates of common multi-drug-resistant pumps had a stronger growth-in...

journal_title：Applied microbiology and biotechnology

authors： Isken S,De Bont JA

更新日期：2000-11-01 00:00:00

abstract：:Cupriavidus basilensis is a species with diverse metabolic capabilities, including degradation of xenobiotics and heavy metal resistance. Although the genomes of several strains of this species have been sequenced, no plasmid has yet been constructed for genetic engineering in this species. In this study, we identifie...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Zhang MN,Bai NL,Ding HT,Zhu XF,Zhao YH

更新日期：2017-02-01 00:00:00

abstract：:Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

journal_title：Applied microbiology and biotechnology

authors： Li B,Wang W,Wang FQ,Wei DZ

更新日期：2010-02-01 00:00:00

abstract：:Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escheric...

journal_title：Applied microbiology and biotechnology

authors： Carlsen S,Ajikumar PK,Formenti LR,Zhou K,Phon TH,Nielsen ML,Lantz AE,Kielland-Brandt MC,Stephanopoulos G

更新日期：2013-07-01 00:00:00