Abstract：
:Relative quantification real-time PCR was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific and eubacterial domain-level primers. Bacterial populations showed a clear predominance of members of the genus Prevotella, which comprised 42% to 60% of the bacterial rRNA gene copies in the samples. However, only 2% to 4% of the bacterial rRNA gene copies were represented by the classical ruminal Prevotella species Prevotella bryantii, Prevotella ruminicola and Prevotella brevis. The proportion of rRNA gene copies attributable to Fibrobacter succinogenes, Ruminococcus flavefaciens, Selenomonas ruminantium and Succinivibrio dextrinosolvens were each generally in the 0.5% to 1% range. Proportions for Ruminobacter amylophilus and Eubacterium ruminantium were lower (0.1% to 0.2%), while Butyrivibrio fibrisolvens, Streptococcus bovis, Ruminococcus albus and Megasphaera elsdenii were even less abundant, each comprising <0.03% of the bacterial rRNA gene copies. The data suggest that the aggregate abundance of the most intensively studied ruminal bacterial species is relatively low and that a large fraction of the uncultured population represents a single bacterial genus.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Stevenson DM,Weimer PJdoi
10.1007/s00253-006-0802-ysubject
Has Abstractpub_date
2007-05-01 00:00:00pages
165-74issue
1eissn
0175-7598issn
1432-0614journal_volume
75pub_type
杂志文章abstract：:The lignin-degrading, biopulping white-rot fungus Physisporinus rivulosus secretes several laccases of distinct features such as thermostability, extremely low pH optima and thermal activation for oxidation of phenolic substrates. Here we describe the cloning, heterologous expression and structural and enzymatic chara...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-012-4011-6
更新日期：2013-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-016-7638-x
更新日期：2016-11-01 00:00:00
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更新日期：2015-04-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2003-08-01 00:00:00
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doi：10.1007/s00253-013-4828-7
更新日期：2013-07-01 00:00:00
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更新日期：2019-02-01 00:00:00
abstract：:In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...
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pub_type： 杂志文章,已发布勘误
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-020-10443-z
更新日期：2020-04-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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abstract：:Lab-scale systems modelling the spontaneous cocoa bean fermentation process are useful tools to research the influence of process parameters on the fermentation and the final bean quality. In this study in Honduras, a 1-kg lab-scale fermentation (LS-F) was compared to a 300-kg on-farm fermentation (OF-F) in a multipha...
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更新日期：2009-10-01 00:00:00
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更新日期：2010-07-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-008-1535-x
更新日期：2008-08-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7635-0
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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