Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil.

abstract：:Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. ...

journal_title：Applied microbiology and biotechnology

authors： Prieto I,Rodríguez MC,Márquez G,Pérez-Aranda A,Barbero JL

更新日期：1992-02-01 00:00:00

abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee J,Majumdar S,Scheper T

更新日期：2000-02-01 00:00:00

abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

journal_title：Applied microbiology and biotechnology

authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

更新日期：1998-05-01 00:00:00

abstract：:Due to their high secretion capacity, Gram-positive bacteria from the genus Bacillus are important expression hosts for the high-yield production of enzymes in industrial biotechnology; however, to date, strains from only few Bacillus species are used for enzyme production at industrial scale. Herein, we introduce Pae...

journal_title：Applied microbiology and biotechnology

authors： Heinze S,Zimmermann K,Ludwig C,Heinzlmeir S,Schwarz WH,Zverlov VV,Liebl W,Kornberger P

更新日期：2018-12-01 00:00:00

abstract：:Microtiter plates were used as minireactors to study Starmerella bombicola growth and sophorolipid (SL) production. Compositional analysis of SL mixtures by liquid chromatography with electrospray ionization tandem mass spectrometry showed similar results on SLs produced using the laboratory scale (shake flask) and th...

journal_title：Applied microbiology and biotechnology

authors： Ribeiro IA,Bronze MR,Castro MF,Ribeiro MH

更新日期：2013-03-01 00:00:00

abstract：:Aspergillus niger var. awamori was previously transformed with a vector designed to express a fused glucoamylase-prochymosin gene and bearing the Neurospora crassa pyr4 gene as a selectable marker. Mutant strains that overproduced the glucoamylase-prochymosin fusion protein were derived from one of the transformants. ...

journal_title：Applied microbiology and biotechnology

authors： Ward M,Wilson LJ,Kodama KH

更新日期：1993-08-01 00:00:00

abstract：:Whey, the main by-product of the cheese industry, is considered as an important pollutant due to its high chemical and biological oxygen demand. Whey, often considered as waste, has high nutritional value and can be used to obtain value-added products, although some of them need expensive enzymatic synthesis. An econo...

journal_title：Applied microbiology and biotechnology

authors： Pescuma M,de Valdez GF,Mozzi F

更新日期：2015-08-01 00:00:00

abstract：:In a previous study, a marine isolate Clostridium sp. EDB2 degraded 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) under anaerobic conditions (Bhushan B, Halasz A, Thiboutot S, Ampleman G, Hawari J (2004c) Chemotaxis-mediated biodegradation of cyclic nitramine explosives RDX, HMX, and CL-20 by Clos...

journal_title：Applied microbiology and biotechnology

authors： Bhushan B,Halasz A,Hawari J

更新日期：2005-12-01 00:00:00

abstract：:The photocatalytic properties of titanium dioxide are well known and have many applications including the removal of organic contaminants and production of self-cleaning glass. There is an increasing interest in the application of the photocatalytic properties of TiO(2) for disinfection of surfaces, air and water. Rev...

journal_title：Applied microbiology and biotechnology

authors： Foster HA,Ditta IB,Varghese S,Steele A

更新日期：2011-06-01 00:00:00

abstract：:Enterobacter sp. strain FL was newly isolated from activated sludge and exhibited significant capability of auto-aggregation as well as aerobic denitrification. The removal efficiencies of NO3--N, total nitrogen (TN), and TOC by strain FL in batch culture reached 94.6, 63.9, and 72.5% in 24 h, respectively. The produc...

journal_title：Applied microbiology and biotechnology

authors： Wang X,An Q,Zhao B,Guo JS,Huang YS,Tian M

更新日期：2018-02-01 00:00:00

abstract：:The worldwide interest of the current era is to increase tendency towards the use of natural substances instead of synthetic ones. So, alternative and effective environment friendly sustainable technologies are highly needed. Due to a broad range of biological activities, fungi are considered as a significant source o...

journal_title：Applied microbiology and biotechnology

authors： Akilandeswari P,Pradeep BV

更新日期：2016-02-01 00:00:00

abstract：:The 16S ribosomal RNA (rRNA) gene has been the most commonly used sequence to characterize bacterial communities. The classical approach to obtain gene sequences to study bacterial diversity implies cloning amplicons, selecting clones, and Sanger sequencing cloned fragments. A more recent approach is direct sequencing...

journal_title：Applied microbiology and biotechnology

authors： Assunção A,Costa MC,Carlier JD

更新日期：2016-03-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:We report here the creation of a modular, plasmid-based protein expression system utilizing elements of the native Rhodobacter puf promoter in a BioBrick(TM)-based vector system with DsRed encoding a red fluorescent reporter protein. A suite of truncations of the puf promoter were made to assess the influence of diffe...

journal_title：Applied microbiology and biotechnology

authors： Tikh IB,Held M,Schmidt-Dannert C

更新日期：2014-04-01 00:00:00

abstract：:Pseudomonas chlororaphis is a plant-associated bacterium with reported antagonistic activity against different organisms and plant growth-promoting properties. P. chlororaphis possesses exciting biotechnological features shared with another Pseudomonas with a nonpathogenic phenotype. Part of the antagonistic role of P...

journal_title：Applied microbiology and biotechnology

authors： Moreno-Avitia F,Utrilla J,Bolívar F,Nogales J,Escalante A

更新日期：2020-12-01 00:00:00

abstract：:Simple phenolic components of wine, hydroxycinnamic acids (HCAs) are known to have antimicrobial properties. This study sought to determine the potential of ferulic acid as an antifungal agent for the control of Dekkera. Growth was inhibited by all HCAs examined in this study, with ferulic acid being the most potent a...

journal_title：Applied microbiology and biotechnology

authors： Harris V,Jiranek V,Ford CM,Grbin PR

更新日期：2010-03-01 00:00:00

abstract：:Microsomal fraction of fungal cells grabs the attention of many researchers for it contains enzymes that play a role in biotechnologically relevant processes. Microsomal enzymes, namely, CYP450s, were shown to metabolize a wide range of xenobiotic compounds, including PAHs, PCBs, dioxins, and endocrine disruptors, and...

journal_title：Applied microbiology and biotechnology

authors： Svobodová K,Mikesková H,Petráčková D

更新日期：2013-12-01 00:00:00

abstract：:Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system. However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota. ...

journal_title：Applied microbiology and biotechnology

authors： Song M,Zeng J,Jia T,Gao H,Zhang R,Jiang J,Li G,Su T

更新日期：2019-11-01 00:00:00

abstract：:Drug-resistant bacteria are becoming an increasingly widespread problem in the clinical setting. The current pipeline of antibiotics cannot provide satisfactory options for clinicians, which brought increasing attention to the development and application of non-traditional antimicrobial substances as alternatives. Met...

journal_title：Applied microbiology and biotechnology

authors： Ye Q,Chen W,Huang H,Tang Y,Wang W,Meng F,Wang H,Zheng Y

更新日期：2020-06-01 00:00:00

abstract：:Based on natural selection and the survival of the fittest by evolutionary adaption, a smart high-throughput system was developed to select active haloalkane dehalogenase variants from a large mutant library. Only active enzyme variants can hydrolyse toxic halogenated alkanes to promote growth, whereas inactive mutant...

journal_title：Applied microbiology and biotechnology

authors： Fibinger MP,Davids T,Böttcher D,Bornscheuer UT

更新日期：2015-11-01 00:00:00

abstract：:Stereoselective carbon-carbon bond formation with aldolases has become an indispensable tool in preparative synthetic chemistry. In particular, the dihydroxyacetone phosphate (DHAP)-dependent aldolases are attractive because four different types are available that allow access to a complete set of diastereomers of vic...

journal_title：Applied microbiology and biotechnology

authors： Schümperli M,Pellaux R,Panke S

更新日期：2007-05-01 00:00:00

abstract：:Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microc...

journal_title：Applied microbiology and biotechnology

authors： Schurig C,Mueller CW,Höschen C,Prager A,Kothe E,Beck H,Miltner A,Kästner M

更新日期：2015-01-01 00:00:00

abstract：:Microbial cell factories offer an economic approach for synthesizing "natural'" aromatic flavor compounds. During their fermentation process, the inefficient synthesis pathway and product cytotoxicity are the major barriers to the high-level production. This study combined metabolic engineering and tolerance engineeri...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-12-01 00:00:00

abstract：:Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fung...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xi Q,Xu Q,He M,Ding J,Dai Y,Keller NP,Zheng W

更新日期：2015-05-01 00:00:00

abstract：:Lactic acid is a versatile organic acid, which finds major application in the food, pharmaceuticals, and chemical industries. Microbial fermentation has the advantage that by choosing a strain of lactic acid bacteria producing only one of the isomers, an optically pure product can be obtained. The production of L: (+)...

journal_title：Applied microbiology and biotechnology

authors： Panesar PS,Kennedy JF,Knill CJ,Kosseva MR

更新日期：2007-02-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permea...

journal_title：Applied microbiology and biotechnology

authors： Pérez-García F,Peters-Wendisch P,Wendisch VF

更新日期：2016-09-01 00:00:00

abstract：:A microbial peptide amidase was found in a limited screening and purified about 500-fold from Stenotrophomonas maltophilia. The native enzyme has a molecular mass of 38 kDa (gel filtration). The sequence of the first 16 amino acids was determined by Edman degradation. The isoelectric point was found to be around 5.8. ...

journal_title：Applied microbiology and biotechnology

authors： Stelkes-Ritter U,Wyzgol K,Kula MR

更新日期：1995-12-01 00:00:00

abstract：:1,3-1,4-beta-D-glucanase is an important endoglycosidase in the brewing and animal feed industries. To achieve high-level expression of recombinant glucanase in Pichia pastoris, we designed sequences encoding the alpha-factor signal peptide from Saccharomyces cerevisiae and the truncated 1,3-1,4-beta-D-glucanase from ...

journal_title：Applied microbiology and biotechnology

authors： Huang H,Yang P,Luo H,Tang H,Shao N,Yuan T,Wang Y,Bai Y,Yao B

更新日期：2008-02-01 00:00:00

abstract：:Human interleukin 6 (hIL-6) overproduced in Escherichia coli HB101 was found to partially retain the initiator methionine (Met) residue (Met-hIL-6). In order to remove the residual N-terminal Met in vivo, an attempt was made to express hIL-6 in aminopeptidase-P (Ap-P)-hyperproducing strains, since the N-terminus Met-P...

journal_title：Applied microbiology and biotechnology

authors： Yasueda H,Kikuchi Y,Kojima H,Nagase K

更新日期：1991-11-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00