Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level.

abstract：:Homologous recombination-based recombineering is a widely used DNA cloning and modification technique; recombineering efficiency improvement would be helpful for high-throughput DNA manipulation. Escherichia coli primase DnaG variants, such as DnaG Q576A and DnaG K580A, increase the recombineering efficiency via impai...

journal_title：Applied microbiology and biotechnology

authors： Li J,Sun J,Gao X,Wu Z,Shang G

更新日期：2019-04-01 00:00:00

abstract：:Pseudomonas sp. has long been known for production of a wide range of secondary metabolites during late exponential and stationary phases of growth. Phenazine derivatives constitute a large group of secondary metabolites produced by microorganisms including Pseudomonas sp. Phenazine 1,6-di-carboxylic acid (PDC) is one...

journal_title：Applied microbiology and biotechnology

authors： Dasgupta D,Kumar A,Mukhopadhyay B,Sengupta TK

更新日期：2015-10-01 00:00:00

abstract：:Plant health is determined by the comprehensive effect of soil physicochemical and biological properties. In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plan...

journal_title：Applied microbiology and biotechnology

authors： Meng T,Wang Q,Abbasi P,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:Despite current remediation efforts, arsenic contamination in water sources is still a major health problem, highlighting the need for new approaches. In this work, strains of the nonpathogenic and highly arsenic-resistant bacterium Corynebacterium glutamicum were used as inexpensive tools to accumulate inorganic arse...

journal_title：Applied microbiology and biotechnology

authors： Villadangos AF,Ordóñez E,Pedre B,Messens J,Gil JA,Mateos LM

更新日期：2014-12-01 00:00:00

abstract：:Rhodovulum sulfidophilum DSM-1374 is a potential producer of polyester when growing in phototrophic conditions. The present study investigated on a polyester product (P3HB) by culturing Rhodovulum sulfidophilum DSM-1374 in two different photobioreactors (PBR-1 and PBR-2) both with 4-L working volumes. PBR-1 is equippe...

journal_title：Applied microbiology and biotechnology

authors： Carlozzi P,Di Lorenzo T,Ghanotakis DF,Touloupakis E

更新日期：2020-03-01 00:00:00

abstract：:The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisa...

journal_title：Applied microbiology and biotechnology

authors： Kudanga T,Le Roes-Hill M

更新日期：2014-08-01 00:00:00

abstract：:In this work, phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of beta-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g beta-sitosterol/l as the unique source of carbon. During 2 months, the bacterial cultures were transferre...

journal_title：Applied microbiology and biotechnology

authors： Vidal M,Becerra J,Mondaca MA,Silva M

更新日期：2001-10-01 00:00:00

abstract：:Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

更新日期：2015-11-01 00:00:00

abstract：:Nutrition-based strategies to optimize xylose to ethanol conversion by Pichia stipitis were identified in growing and stationary-phase cultures provided with a defined medium varied in nitrogen, vitamin, purine/pyrimidine, and mineral content via full or partial factorial designs. It is surprising to note that station...

journal_title：Applied microbiology and biotechnology

authors： Slininger PJ,Dien BS,Gorsich SW,Liu ZL

更新日期：2006-10-01 00:00:00

abstract：:Panax ginseng C.A. Meyer (ginseng) is a well-known medicinal plant that has been traditionally used in the oriental countries for centuries. Wild ginseng is a scarce and rare commodity. Field cultivation of the ginseng plant is a time-consuming and labor-intensive process. Ginsenosides, a group of glycosylated triterp...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Georgiev MI,Kim YS,Jeong CS,Kim SJ,Park SY,Paek KY

更新日期：2014-01-01 00:00:00

abstract：:Nonribosomal peptides (NRPs), a large family of natural products, possess numerous pharmaceutically significant bioactivities. However, many native microbial producers of NRPs are not cultivable or have low production yields making mass production infeasible. The recombinant production of natural products in a surroga...

journal_title：Applied microbiology and biotechnology

authors： Li J,Jaitzig J,Hillig F,Süssmuth R,Neubauer P

更新日期：2014-01-01 00:00:00

abstract：:Alternative ligands such as nucleic acid aptamers can be used for pathogen capture and detection and offer advantages over antibodies, including reduced cost, ease of production and modification, and improved stability. DNA aptamers demonstrating binding specificity to Salmonella enterica serovar Typhimurium were iden...

journal_title：Applied microbiology and biotechnology

authors： Dwivedi HP,Smiley RD,Jaykus LA

更新日期：2013-04-01 00:00:00

abstract：:Paddy fields are a significant source of methane and contribute up to 20% of total methane emissions from wetland ecosystems. These inundated, anoxic soils featuring abundant nitrogen compounds and methane are an ideal niche for nitrate-dependent anaerobic methanotrophs. After 2 years of enrichment with a continuous s...

journal_title：Applied microbiology and biotechnology

authors： Vaksmaa A,Guerrero-Cruz S,van Alen TA,Cremers G,Ettwig KF,Lüke C,Jetten MSM

更新日期：2017-09-01 00:00:00

abstract：:Cell growth needs to be monitored in biological studies and bioprocess optimization. In special circumstances, such as microbial fermentations in media containing insoluble particles, accurate cell growth quantification is a challenge with current methods. Only the Burton method is applicable in such circumstances. Th...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xiang S,Dai X,Yang K

更新日期：2013-06-01 00:00:00

abstract：:The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, was found to be highly effective against a broad range of fungal plant pathogens. Current understanding of the regulatory mechanism of rimocidin biosynthesis and morphological differentiation in S. rimosus M527 is limited. NsdA is considered a neg...

journal_title：Applied microbiology and biotechnology

authors： Liao Z,Song Z,Xu J,Ma Z,Bechthold A,Yu X

更新日期：2020-12-01 00:00:00

abstract：:Fermentation-derived products are in greater demand to meet the increasing global market as well as to overcome environmental problems. In this work, Escherichia coli has been metabolically engineered with acrylate pathway genes from Clostridium propionicum for the conversion of D-lactic acid to propionic acid. The in...

journal_title：Applied microbiology and biotechnology

authors： Kandasamy V,Vaidyanathan H,Djurdjevic I,Jayamani E,Ramachandran KB,Buckel W,Jayaraman G,Ramalingam S

更新日期：2013-02-01 00:00:00

abstract：:Ammonium can be removed as dinitrogen gas by cooperating aerobic and anaerobic ammonium-oxidizing bacteria (AerAOB and AnAOB). The goal of this study was to verify putative mutual benefits for aggregated AerAOB and AnAOB in a stagnant freshwater environment. In an ammonium fed water column, the biological oxygen consu...

journal_title：Applied microbiology and biotechnology

authors： Vlaeminck SE,Dierick K,Boon N,Verstraete W

更新日期：2007-07-01 00:00:00

abstract：:A cytochrome P450 and an iron-sulfur protein, whose expression was specifically induced during growth of Mycobacterium sp. strain HE5 on morpholine as the sole source of carbon, nitrogen, and energy were purified to apparent homogeneity. Due to the lack of enzymatic activity, carbon monoxide difference spectra and det...

journal_title：Applied microbiology and biotechnology

authors： Sielaff B,Andreesen JR,Schräder T

更新日期：2001-08-01 00:00:00

abstract：:Neuritin (also known as candidate plasticity gene 15 (cpg15)) is a neurotrophic factor that was recently discovered in a screen aimed at identifying genes involved in activity-dependent synaptic plasticity. Neuritin plays multiple roles in both neural development (Chen et al. Zhonghua Yan Ke Za Zhi 46:978-983 2010; Co...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Zhang S,Xian L,Tang J,Zhu J,Cui L,Li S,Yang L,Huang J

更新日期：2015-10-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00

abstract：:This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

journal_title：Applied microbiology and biotechnology

authors： Albadran HA,Monteagudo-Mera A,Khutoryanskiy VV,Charalampopoulos D

更新日期：2020-07-01 00:00:00

abstract：:Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

journal_title：Applied microbiology and biotechnology

authors： Rui L,Reardon KF,Wood TK

更新日期：2005-01-01 00:00:00

abstract：:We report the successful high-yield expression of Candida utilis uricase in Escherichia coli and the establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein, which was confirmed to be C. utilis uricase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis an...

journal_title：Applied microbiology and biotechnology

authors： Liu X,Wen M,Li J,Zhai F,Ruan J,Zhang L,Li S

更新日期：2011-11-01 00:00:00

abstract：:The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

journal_title：Applied microbiology and biotechnology

authors： Lu XM,Lu PZ

更新日期：2014-09-01 00:00:00

abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

journal_title：Applied microbiology and biotechnology

authors： Alonso S,Rendueles M,Díaz M

更新日期：2012-12-01 00:00:00

abstract：:In this work, fermentation and formulation aspects of the nematophagous fungus Hirsutella rhossiliensis BBA were investigated. When incubated in 2% (w/w) glucose and 0.5% (w/w) yeast extract medium in a 1-L Erlenmeyer flask without baffles, heavy pellet formation was observed. Only 40% of the mycelium had a size less ...

journal_title：Applied microbiology and biotechnology

authors： Patel AV,Jakobs-Schönwandt D,Rose T,Vorlop KD

更新日期：2011-03-01 00:00:00

abstract：:The quorum sensing signalling molecules 2-heptyl-3-hydroxy-4(1H)-quinolone, termed the "Pseudomonas quinolone signal" (PQS), and 2-heptyl-4(1H)-quinolone (HHQ) play an important role in the control of virulence gene expression in Pseudomonas aeruginosa. To construct a bioreporter for the specific and sensitive detecti...

journal_title：Applied microbiology and biotechnology

authors： Müller C,Fetzner S

更新日期：2013-01-01 00:00:00

abstract：:Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

journal_title：Applied microbiology and biotechnology

authors： Misra G,Pavlostathis SG,Perdue EM,Araujo R

更新日期：1996-07-01 00:00:00

abstract：:Stable transformants of Chromobacterium violaceum were obtained by high-voltage electroporation with a 7-kilobase binary plasmid. The technique was reliable, reproducible, and simple, with efficiencies of 10(5) transformants/microg of plasmid DNA. The electrical conditions that resulted in the highest efficiencies wer...

journal_title：Applied microbiology and biotechnology

authors： Broetto L,Cecagno R,Sant'anna FH,Weber S,Schrank IS

更新日期：2006-07-01 00:00:00