Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution.

abstract：:Exophiala jeanselmei is an opportunistic pathogenic black yeast growing in humid environments such as water reservoirs of air-conditioning systems. Because this fungal contaminant could be vaporized into the air and subsequently cause health problems, its monitoring is recommended. Currently, this monitoring is based ...

journal_title：Applied microbiology and biotechnology

authors： Libert X,Chasseur C,Packeu A,Bureau F,Roosens NH,De Keersmaecker SJC

更新日期：2016-02-01 00:00:00

abstract：:Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

journal_title：Applied microbiology and biotechnology

authors： Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

更新日期：2013-08-01 00:00:00

abstract：:Pneumocandin B0, the precursor of the antifungal drug caspofungin, is a secondary metabolite of the fungus Glarea lozoyensis. In this study, we investigated the effects of mannitol as the sole carbon source on pneumocandin B0 production by G. lozoyensis. The osmotic pressure is more important in enhancing pneumocandin...

journal_title：Applied microbiology and biotechnology

authors： Song P,Huang B,Zhang S,Zhang K,Yuan K,Ji X,Ren L,Wen J,Huang H

更新日期：2018-12-01 00:00:00

abstract：:To measure the toxicity experienced by superoxide-generating compounds, two plasmids were constructed in which the superoxide-inducible fpr and zwf promoters from Escherichia coli were fused to promoterless Vibrio fischeri luxCDABE operon present in plasmid pUCD615. The bioluminescent response of E. coli harboring the...

journal_title：Applied microbiology and biotechnology

authors： Niazi JH,Kim BC,Gu MB

更新日期：2007-04-01 00:00:00

abstract：:Lyme borreliosis is a bacterial infection that can be spread to humans by infected ticks and may severely affect many organs and tissues. Nearly four decades have elapsed since the discovery of the disease agent called Borrelia burgdorferi. Although there is a plethora of knowledge on the infectious agent and thousand...

journal_title：Applied microbiology and biotechnology

authors： Strnad M,Grubhoffer L,Rego ROM

更新日期：2020-03-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii is a well-documented pathogen associated with hospital-acquired infections. In addition to multidrug resistance, A. baumannii can also become resistant to colistin, the antibiotic treatment of last resort, by the loss of the lipopolysaccharide from its outer membrane. Here, ...

journal_title：Applied microbiology and biotechnology

authors： Tseng CC,Tsai YH,Hu A,Liou JW,Chang KC,Chang HH

更新日期：2016-10-01 00:00:00

abstract：:L-Asparaginases (EC 3.5.1.1) are enzymes that catalyze the hydrolysis of L-asparagine to L-aspartic acid and found in a variety of organisms from microorganisms to mammals. However, they are mainly expressed and produced by microorganisms. Microbial L-asparaginases have received sustained attention due to their irrepl...

journal_title：Applied microbiology and biotechnology

authors： Zuo S,Zhang T,Jiang B,Mu W

更新日期：2015-02-01 00:00:00

abstract：:The microbes present in bioethanol production processes have been previously studied in laboratory-scale experiments, but there is a lack of information on full-scale industrial processes. In this study, the microbial communities of three industrial bioethanol production processes were characterized using several meth...

journal_title：Applied microbiology and biotechnology

authors： Aho VTE,Tolonen T,Haverinen J,Jaakkola M,Paulin L,Auvinen P,Laine MM

更新日期：2020-09-01 00:00:00

abstract：:Wastewater treatment plants (WWTPs) are major collection pools of antibiotics of which low concentrations may induce antibiotic resistance in their microbial communities and pose threat to human health. However, information is still limited on the microbial community alteration in WWTPs upon exposure to low-dose antib...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Fan C,Low A,He J

更新日期：2014-03-01 00:00:00

abstract：:A set of mutants was generated by targeted deletion of the hsdR loci of two type I restriction modification systems (RMS) identified in Bacillus licheniformis DSM13. Single as well as double knock-outs resulted in strains being readily transformable with plasmids isolated from Bacilli. Introduction of shuttle plasmids...

journal_title：Applied microbiology and biotechnology

authors： Waschkau B,Waldeck J,Wieland S,Eichstädt R,Meinhardt F

更新日期：2008-02-01 00:00:00

abstract：:Polymalic acid (PMA) is a biodegradable polymer produced by the polyextremotolerant fungi Aureobasidium pullulans and has been shown to have potential applications in environmental fields. In this work, a high PMA yield mutant FJ-D2 was screened from T-DNA-based mutant libraries and showed a 12.9% increase in PMA tite...

journal_title：Applied microbiology and biotechnology

authors： Feng J,Li T,Zhang X,Chen J,Zhao T,Zou X

更新日期：2019-08-01 00:00:00

abstract：:The fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete Coriolus versicolor. The fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. Under primary metabolic conditions, C. v...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Nakamizo M,Wariishi H,Tanaka H

更新日期：2002-03-01 00:00:00

abstract：:Brazzein is an intensely sweet-tasting plant protein with good stability, which makes it an attractive alternative to sucrose. A brazzein gene has been designed, synthesized, and expressed in Escherichia coli at 30 degrees C to yield brazzein in a soluble form and in considerable quantity. Antibodies have been produce...

journal_title：Applied microbiology and biotechnology

authors： Berlec A,Jevnikar Z,Majhenic AC,Rogelj I,Strukelj B

更新日期：2006-11-01 00:00:00

abstract：:Due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4'-trihydroxystilbene) has attracted great research interest. The objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol productio...

journal_title：Applied microbiology and biotechnology

authors： Shi J,Zeng Q,Liu Y,Pan Z

更新日期：2012-07-01 00:00:00

abstract：:Lipoxygenase (LOX; EC 1.13.11.12) is an enzyme which is widely used in food industry to improve aroma and rheological or baking properties of foods. A series of studies have proven that the flexible regions negatively relates to the thermal stability of enzymes. In this study, two highly flexible regions, residues(20-...

journal_title：Applied microbiology and biotechnology

authors： Lu X,Liu S,Feng Y,Rao S,Zhou X,Wang M,Du G,Chen J

更新日期：2014-02-01 00:00:00

abstract：:Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry w...

journal_title：Applied microbiology and biotechnology

authors： Bengtsson L,Johansson B,Hackett TJ,McHale L,McHale AP

更新日期：1995-01-01 00:00:00

abstract：:Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetica...

journal_title：Applied microbiology and biotechnology

authors： Wang JJ,Cai Q,Qiu L,Ying SH,Feng MG

更新日期：2018-02-01 00:00:00

abstract：:There is an error in the Original Publication of this paper for "Acknowledgements" section was missing. ...

journal_title：Applied microbiology and biotechnology

authors： Hauschild P,Röttig A,Madkour MH,Al-Ansari AM,Almakishah NH,Steinbüchel A

更新日期：2020-03-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:In natural settings, anaerobic digestion can take place in a wide temperature range, but industrial digesters are usually operated under either mesophilic (~35 °C) or thermophilic (~55 °C) conditions. The ability of anaerobic digestion microbiota to switch from one operating temperature to the other remains poorly doc...

journal_title：Applied microbiology and biotechnology

authors： Chapleur O,Mazeas L,Godon JJ,Bouchez T

更新日期：2016-02-01 00:00:00

abstract：:Pneumocandin B0 is a hydrophobic secondary metabolite that accumulates in the mycelia of Glarea lozoyensis and inhibits fungal 1,3-β-glucan synthase. Extractive batch fermentation can promote the release of intracellular secondary metabolites into the fermentation broth and is often used in industry. The addition of e...

journal_title：Applied microbiology and biotechnology

authors： Yuan K,Huang B,Qin T,Song P,Zhang K,Ji X,Ren L,Zhang S,Huang H

更新日期：2019-08-01 00:00:00

abstract：:Cupriavidus basilensis is a species with diverse metabolic capabilities, including degradation of xenobiotics and heavy metal resistance. Although the genomes of several strains of this species have been sequenced, no plasmid has yet been constructed for genetic engineering in this species. In this study, we identifie...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Zhang MN,Bai NL,Ding HT,Zhu XF,Zhao YH

更新日期：2017-02-01 00:00:00

abstract：:Human immunodeficiency virus (HIV)/AIDS continues to spread worldwide, and most of the HIV-infected people living in developing countries have little or no access to highly active antiretroviral therapy. The development of efficient and low-cost microbicides to prevent sexual transmission of HIV should be given high p...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Fan J,Kitazato K

更新日期：2010-04-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:C (2) to C (4) chloroalkanes have been used for a wide range of industrial applications. Consequently, numerous leaks to the environment have occurred. It is generally observed that the lower chlorinated members of the group, containing 1-3 chlorine atoms, accumulate in environments where reductive conditions prevail....

journal_title：Applied microbiology and biotechnology

authors： De Wildeman S,Verstraete W

更新日期：2003-04-01 00:00:00

abstract：:The effect of sodium chloride (NaCl) on pullulan production by batch culture of Aureobasidium pullulans CCTCC M 2012259 was investigated. NaCl at 3 g/L improved the pullulan titer by 26.7% but reduced the molecular weight of pullulan to only 46.8% of that obtained in the control without NaCl. In order to elucidate the...

journal_title：Applied microbiology and biotechnology

authors： Wang DH,Ni TF,Ju XM,Wei GY

更新日期：2018-10-01 00:00:00

abstract：:Twenty-six species of Phellinus sensu lato, reported as medicinal mushrooms, are enumerated in this review. The species' names were checked and revised according to contemporary taxonomy and the latest version of the International Code of Botanical Nomenclature (Vienna Code); two misapplied names of Phellinus baumii P...

journal_title：Applied microbiology and biotechnology

authors： Dai YC,Zhou LW,Cui BK,Chen YQ,Decock C

更新日期：2010-08-01 00:00:00

abstract：:A histidine oligopeptide (hexa-His) with the ability to chelate divalent heavy metal ions was displayed on the yeast cell surface for the purpose of enhanced adsorption of heavy metal ions. We genetically fused a hexa-His-encoding gene with the gene encoding the C-terminal half of alpha-agglutinin that includes a glyc...

journal_title：Applied microbiology and biotechnology

authors： Kuroda K,Shibasaki S,Ueda M,Tanaka A

更新日期：2001-12-01 00:00:00

abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

journal_title：Applied microbiology and biotechnology

authors： Seo MH,Kim KR,Oh DK

更新日期：2013-10-01 00:00:00