Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis.

abstract：:Ammonium can be removed as dinitrogen gas by cooperating aerobic and anaerobic ammonium-oxidizing bacteria (AerAOB and AnAOB). The goal of this study was to verify putative mutual benefits for aggregated AerAOB and AnAOB in a stagnant freshwater environment. In an ammonium fed water column, the biological oxygen consu...

journal_title：Applied microbiology and biotechnology

authors： Vlaeminck SE,Dierick K,Boon N,Verstraete W

更新日期：2007-07-01 00:00:00

abstract：:Exposure to bioaerosols can adversely influence human health through respiratory tract, eye, and skin irritation. Bioaerosol composition is unique on the International Space Station (ISS), where the size distribution of particles in the air differs from those on Earth. This is due to the lack of gravitational settling...

journal_title：Applied microbiology and biotechnology

authors： Haines SR,Bope A,Horack JM,Meyer ME,Dannemiller KC

更新日期：2019-09-01 00:00:00

abstract：:Porcine parvovirus type 1 (PPV1) is a major causative agent of embryonic and fetal death in swine. The PPV1 VP2 protein is closely associated with viral immunogenicity for eliciting neutralizing antibodies, but its antigenic structures have been largely unknown. We generated three monoclonal antibodies (MAbs) against ...

journal_title：Applied microbiology and biotechnology

authors： Sun J,Huang L,Wei Y,Wang Y,Chen D,Du W,Wu H,Feng L,Liu C

更新日期：2015-11-01 00:00:00

abstract：:A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose conce...

journal_title：Applied microbiology and biotechnology

authors： Santos LO,Gonzales TA,Ubeda BT,Monte Alegre R

更新日期：2007-12-01 00:00:00

abstract：:Microbial deterioration accounts for a significant percentage of the degradation processes that occur on archeological/historical objects and artworks, and identifying the causative agents of such a phenomenon should therefore be a priority, in consideration of the need to conserve these important cultural heritage it...

journal_title：Applied microbiology and biotechnology

authors： Mazzoli R,Giuffrida MG,Pessione E

更新日期：2018-08-01 00:00:00

abstract：:Glycerol is used by the cosmetic, paint, automotive, food, and pharmaceutical industries and for production of explosives. Currently, glycerol is available in commercial quantities as a by-product from biodiesel production, but the purity and the cost of its purification are prohibitive. The industrial production of g...

journal_title：Applied microbiology and biotechnology

authors： Semkiv MV,Dmytruk KV,Abbas CA,Sibirny AA

更新日期：2017-06-01 00:00:00

abstract：:One of the most important limitations of mammalian cell-based processes is the secretion and accumulation of lactate as a by-product of their metabolism. Among the cell lines commonly used in industrial bioprocesses, HEK293 has been gaining importance over the last years. Up recently, HEK293 cells were known to consum...

journal_title：Applied microbiology and biotechnology

authors： Liste-Calleja L,Lecina M,Lopez-Repullo J,Albiol J,Solà C,Cairó JJ

更新日期：2015-12-01 00:00:00

abstract：:With their ability to lyse Gram-positive bacteria, phage lytic enzymes (or lysins) have received a great deal of attention as novel anti-infective agents. The number of known genes encoding these peptidoglycan hydrolases has increased markedly in recent years, due in large part to advances in DNA sequencing technology...

journal_title：Applied microbiology and biotechnology

authors： Schmitz JE,Ossiprandi MC,Rumah KR,Fischetti VA

更新日期：2011-03-01 00:00:00

abstract：:Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters, the one for alcohol dehydrogenase (PadhE) was found most effective. Recombinant E. coli JM 109 (pWCY09) ha...

journal_title：Applied microbiology and biotechnology

authors： Wei XX,Shi ZY,Yuan MQ,Chen GQ

更新日期：2009-03-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

journal_title：Applied microbiology and biotechnology

authors： Dillon AJ,Zorgi C,Camassola M,Henriques JA

更新日期：2006-05-01 00:00:00

abstract：:In insects, glutathione S-transferases (GSTs) are enzymes involved in detoxification of insecticides. However, few data are available for the codling moth, Cydia pomonella (L.). In this study, we cloned a delta class GST gene CpGSTd1 from C. pomonella. Real-time quantitative PCR shows that CpGSTd1 was up-regulated wit...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Yang X,Zhang Y

更新日期：2014-11-01 00:00:00

abstract：:Surface sediments are the inner source of contaminations in aquatic systems and usually maintain aerobic conditions. As the key participators of nitrification process, little is known about the activities and contributions of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in the surface sediments. In this study, w...

journal_title：Applied microbiology and biotechnology

authors： Dang C,Liu W,Lin Y,Zheng M,Jiang H,Chen Q,Ni J

更新日期：2018-04-01 00:00:00

abstract：:Despite airborne microorganisms representing a relevant fraction of atmospheric suspended particles, only a small amount of information is currently available on their abundance and diversity and very few studies have investigated the environmental factors influencing the structure of airborne bacterial communities. I...

journal_title：Applied microbiology and biotechnology

authors： Bertolini V,Gandolfi I,Ambrosini R,Bestetti G,Innocente E,Rampazzo G,Franzetti A

更新日期：2013-07-01 00:00:00

abstract：:Recently, a new gene encoding beta-glucuronidase from Streptococcus equi subsp. zooepidemicus (SEZ) was identified and expressed in Escherichia coli. In this paper, the characterization of the enzyme is described. Specific enzyme activity was 120,000 U/mg purified protein at 37 degrees C and pH = 7.0. The temperature ...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Krahulcová J

更新日期：2007-04-01 00:00:00

abstract：:Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

journal_title：Applied microbiology and biotechnology

authors： Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

更新日期：2017-11-01 00:00:00

abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

journal_title：Applied microbiology and biotechnology

authors： Vinuselvi P,Lee SK

更新日期：2011-10-01 00:00:00

abstract：:The cell envelope-associated proteinase (CEP) of Lactobacillus delbrueckii subsp. lactis CRL 581 (PrtL) has an essential role in bacterial growth, contributes to the flavor and texture development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. The genome of L. del...

journal_title：Applied microbiology and biotechnology

authors： Villegas JM,Brown L,Savoy de Giori G,Hebert EM

更新日期：2015-05-01 00:00:00

abstract：:The main objective of this work was the optimization of the production of the beta-ketolase, acetopyruvate hydrolase, from Pseudomonas putida O1. Orcinol was used as an inducer for enzyme production. The growth medium was optimized in two steps. In the first step, screening for optimal glucose concentration was perfor...

journal_title：Applied microbiology and biotechnology

authors： Hofer H,Mandl T,Steiner W

更新日期：2002-11-01 00:00:00

abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

journal_title：Applied microbiology and biotechnology

authors： Wemhoff S,Meinhardt F

更新日期：2013-09-01 00:00:00

abstract：:Rhodococcus erythropolis cells contain a large set of enzymes that allow them to carry out an enormous number of bioconversions and degradations. Oxidations, dehydrogenations, epoxidations, hydrolysis, hydroxylations, dehalogenations and desulfurisations have been reported to be performed by R. erythropolis cells or e...

journal_title：Applied microbiology and biotechnology

authors： de Carvalho CC,da Fonseca MM

更新日期：2005-06-01 00:00:00

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:Clostridium acetobutylicum is an important industrial microorganism for n-butanol bioproduction, and its transcription factor AbrB0310 regulates various important cellular processes. However, the roles of two abrB homologues, abrB1941 and abrB3647, have not been determined because they appear inactive during transcrip...

journal_title：Applied microbiology and biotechnology

authors： Xue Q,Yang Y,Chen J,Chen L,Yang S,Jiang W,Gu Y

更新日期：2016-11-01 00:00:00

abstract：:Hyaluronic acid (HA) is a ubiquitous polysaccharide found in humans, animals, bacteria, algae and molluscs. Simple yet sophisticated, HA demonstrates unique and valuable rheological properties. In solution, HA behaves as a stiffened random coil and the resultant behaviour, even at low concentrations, is far from Newto...

journal_title：Applied microbiology and biotechnology

authors： Marcellin E,Steen JA,Nielsen LK

更新日期：2014-08-01 00:00:00

abstract：:N-acetyl glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine that is inhibited by its end product L-arginine in Corynebacterium glutamicum (C. glutamicum). In this study, the potential binding sites of arginine and the residues essential for its inhibition were identified by homology modeling, inhib...

journal_title：Applied microbiology and biotechnology

authors： Huang Y,Zhang H,Tian H,Li C,Han S,Lin Y,Zheng S

更新日期：2015-09-01 00:00:00

abstract：:Inonotus obliquus (Fr.) Pilat is a white rot fungus belonging to the family Hymenochaetaceae in the Basidiomycota. In nature, this fungus rarely forms a fruiting body but usually an irregular shape of sclerotial conk called 'Chaga'. Characteristically, I. obliquus produces massive melanins released to the surface of C...

journal_title：Applied microbiology and biotechnology

authors： Zheng W,Miao K,Liu Y,Zhao Y,Zhang M,Pan S,Dai Y

更新日期：2010-07-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:In microorganisms, the enzyme acetate kinase (AK) catalyses the formation of ATP from ADP by de-phosphorylation of acetyl phosphate into acetic acid. A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type. In th...

journal_title：Applied microbiology and biotechnology

authors： Kuit W,Minton NP,López-Contreras AM,Eggink G

更新日期：2012-05-01 00:00:00

abstract：:Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

journal_title：Applied microbiology and biotechnology

authors： Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

更新日期：2013-08-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00