Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain.

abstract：:Malate synthase (Mls), a key enzyme in the glyoxylate cycle, is required for virulence in microbial pathogens. In this study, we identified the AoMls gene from the nematode-trapping fungus Arthobotrys oligospora. The gene contains 4 introns and encodes a polypeptide of 540 amino acids. To characterize the function of ...

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Wang Y,Zhao Y,Huang Y,Zhang KQ,Yang J

更新日期：2014-03-01 00:00:00

abstract：:A fast and convenient bacterial immobilization method was proposed as an attempt to improve the anode efficiency of a microbial fuel cell, in which bacteria were entrapped into carbon nanoparticle matrix. The direct electron transfer from the entrapped bacterial cells to the anode was verified using cyclic voltammogra...

journal_title：Applied microbiology and biotechnology

authors： Yuan Y,Zhou S,Xu N,Zhuang L

更新日期：2011-03-01 00:00:00

abstract：:This study investigated the characterization and flocculation mechanism of a bioflocculant prepared using potato starch wastewater. The optimal culture conditions of this strain were determined as 4 g K2HPO4, 2 g KH2PO4, 0.2 g MgSO4, 0.1 g NaCl, and 2.0 g urea dissolved in 1.0 L potato starch wastewater with no need o...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Lau AK,Zhang Y,Zhao J

更新日期：2015-07-01 00:00:00

abstract：:Chiral alcohols are valuable as diverse chemicals and synthetic intermediate materials. Phenylacetaldehyde reductase (PAR) is an enzyme that converts a wide variety of ketones into chiral alcohols with high optical purity. When an alcohol such as 2-propanol is used as a hydrogen donor, PAR itself will also mediate the...

journal_title：Applied microbiology and biotechnology

authors： Makino Y,Itoh N

更新日期：2014-05-01 00:00:00

abstract：:Natural genetic competence enables bacteria to take in and establish exogenously supplied DNA and thus constitutes a valuable tool for strain improvement. Extensively studied in the Gram-positive model organism Bacillus subtilis genetic competence has indeed proven successful for genetic manipulation aiming at enhance...

journal_title：Applied microbiology and biotechnology

authors： Jakobs M,Meinhardt F

更新日期：2015-02-01 00:00:00

abstract：:Clonorchis sinensis (C. sinensis), an important fishborne zoonotic parasite threatening public health, is of major socioeconomic importance in epidemic areas. Effective strategies are still urgently expected to prevent against C. sinensis infection. In the present study, paramyosin of C. sinensis (CsPmy) was stably an...

journal_title：Applied microbiology and biotechnology

authors： Sun H,Shang M,Tang Z,Jiang H,Dong H,Zhou X,Lin Z,Shi C,Ren P,Zhao L,Shi M,Zhou L,Pan H,Chang O,Li X,Huang Y,Yu X

更新日期：2020-02-01 00:00:00

abstract：:Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry w...

journal_title：Applied microbiology and biotechnology

authors： Bengtsson L,Johansson B,Hackett TJ,McHale L,McHale AP

更新日期：1995-01-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00

abstract：:Cupriavidus basilensis is a species with diverse metabolic capabilities, including degradation of xenobiotics and heavy metal resistance. Although the genomes of several strains of this species have been sequenced, no plasmid has yet been constructed for genetic engineering in this species. In this study, we identifie...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Zhang MN,Bai NL,Ding HT,Zhu XF,Zhao YH

更新日期：2017-02-01 00:00:00

abstract：:Our previous work showed that there is a limitation in the use of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification systems in dhfr-non-deficient HEK293 cells, as endogenous dhfr may interfere with the amplification process. In the present study, we successfully generated Epstein-Barr virus...

journal_title：Applied microbiology and biotechnology

authors： Park SH,Park JH,Lee JH,Lee HM,Kang YJ,Lee EJ,Shin S,Lee GM,Kim YG

更新日期：2021-01-01 00:00:00

abstract：:The efficient production of recombinant proteins such as antibodies typically involves the screening of an extravagant number of clones in order to finally select a stable and high-producing cell line. Thereby, the underlying principles of a powerful protein machinery, but also potential expression limitations, often ...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Sommeregger W,Debreczeny M,Gludovacz E,Kunert R

更新日期：2014-07-01 00:00:00

abstract：:Certain lactic acid bacteria, especially heterofermentative strains, are capable to produce mannitol under adequate culture conditions. In this study, mannitol production by Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in modified MRS medium containing a mixture of fructose and glucose in a 6.5:1...

journal_title：Applied microbiology and biotechnology

authors： Rodríguez C,Rimaux T,Fornaguera MJ,Vrancken G,de Valdez GF,De Vuyst L,Mozzi F

更新日期：2012-03-01 00:00:00

abstract：:A bacterial strain C21 isolated from constructed wetland soil was identified as Arthrobacter sp. based on 16S rRNA gene sequence analysis and physio-biochemical characteristics and was capable of utilizing di-n-butyl phthalate (DBP) as a carbon and energy source for growth. Strain C21 can also utilize other phthalates...

journal_title：Applied microbiology and biotechnology

authors： Wen ZD,Gao DW,Wu WM

更新日期：2014-05-01 00:00:00

abstract：:L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

journal_title：Applied microbiology and biotechnology

authors： Ochsenreither K,Fischer C,Neumann A,Syldatk C

更新日期：2014-06-01 00:00:00

abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

journal_title：Applied microbiology and biotechnology

authors： Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

更新日期：2017-01-01 00:00:00

abstract：:The first two steps in xylose metabolism are catalyzed by NAD(P)H-dependent xylose reductase (XR) (EC 1.1.1.21) and NAD(P)-dependent xylitol dehydrogenase (XDH) (EC 1.1.1.9), which lead to xylose-->xylitol-->xylulose conversion. Xylitol has high commercial value, due to its sweetening and anticariogenic properties, as...

journal_title：Applied microbiology and biotechnology

authors： Lima LH,das Graças de Almeida Felipe M,Vitolo M,Torres FA

更新日期：2004-11-01 00:00:00

abstract：:The sheep rumen microbial community plays an important role in animal performance and the environment. Few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. A total of 112 healthy Tan sheep were selected and randomly allotted to one of four dietary...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Cao P,Wang L,Zhao Z,Chen Y,Yang Y

更新日期：2017-05-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:Blowfly (Diptera: Calliphoridae) species Lucilia sericata (Meigen) and related species Lucilia cuprina (Wiedmann) are important agricultural pests, assist in forensic fields and also have a therapeutic role in medicine. Both species (though predominantly L. sericata) are utilised in a clinical setting for maggot debri...

journal_title：Applied microbiology and biotechnology

authors： Gasz NE,Geary MJ,Doggett SL,Harvey ML

更新日期：2021-01-07 00:00:00

abstract：:Although a large number of AroA enzymes (5-enopyruvylshikimate-3-phosphate synthase [EPSPS]) have been identified, cloned and tested for glyphosate resistance, only AroA variants derived from Agrobacterium tumefaciens strain CP4 have been successfully used commercially. We have now used a polymerase chain reaction (PC...

journal_title：Applied microbiology and biotechnology

authors： Tian YS,Xu J,Xiong AS,Zhao W,Gao F,Fu XY,Peng RH,Yao QH

更新日期：2012-01-01 00:00:00

abstract：:A novel immobilisation design for increasing the final concentration of the heterologous protein lysozyme by a genetically engineered fungus, Aspergillus niger B1, was developed. A central composition design was used to investigate different immobilised polymer types (alginate and pectate), polymer concentration [24% ...

journal_title：Applied microbiology and biotechnology

authors： Parra R,Aldred D,Magan N

更新日期：2005-05-01 00:00:00

abstract：:Corynebacterium glutamicum, a high GC content gram-positive soil bacterium in Actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. Since its genome sequence was first published, its versatile metabolic pa...

journal_title：Applied microbiology and biotechnology

authors： Toyoda K,Inui M

更新日期：2016-01-01 00:00:00

abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

journal_title：Applied microbiology and biotechnology

authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

更新日期：2010-08-01 00:00:00

abstract：:Two cephalosporin genes from Acremonium chrysogenum, pcbAB and pcbC encode the ACV (alpha-aminoadipyl-cysteinyl-valine) synthetase and isopenicillin N-synthetase, respectively. The two adjacent genes are orientated in opposite directions on the chromosomal DNA, separated by a 1.2-kb non-translated sequence, carrying t...

journal_title：Applied microbiology and biotechnology

authors： Menne S,Walz M,Kück U

更新日期：1994-10-01 00:00:00

abstract：:There are alarming reports of growing microbial resistance to all classes of antimicrobial agents used against different infections. Also the existing classes of anticancer drugs used against different tumours warrant the urgent search for more effective alternative agents for treatment. Broad-spectrum bioactivities o...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Kon K,Ingle A,Duran N,Galdiero S,Galdiero M

更新日期：2014-03-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechoc...

journal_title：Applied microbiology and biotechnology

authors： Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

更新日期：2008-03-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

journal_title：Applied microbiology and biotechnology

authors： Shen G,Zhang S,Liu X,Jiang Q,Ding W

更新日期：2018-11-01 00:00:00

abstract：:An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temper...

journal_title：Applied microbiology and biotechnology

authors： Singh RK,Tiwari MK,Kim D,Kang YC,Ramachandran P,Lee JK

更新日期：2013-08-01 00:00:00