Improved production of dibenzocyclooctadiene lignans in the elicited microshoot cultures of Schisandra chinensis (Chinese magnolia vine).

abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

journal_title：Applied microbiology and biotechnology

authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

更新日期：1998-05-01 00:00:00

abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

journal_title：Applied microbiology and biotechnology

authors： Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

更新日期：2015-07-01 00:00:00

abstract：:Red koji or red mold rice is prepared by growing a genus Monascus on steamed rice. For centuries, it has been used in Asia for the production of fermented foods including red rice wine and fermented tofu. Although red koji is an important source of various hydrolytic enzymes critical for food fermentation, information...

journal_title：Applied microbiology and biotechnology

authors： Yasuda M,Tachibana S,Kuba-Miyara M

更新日期：2012-10-01 00:00:00

abstract：:A beta-lactamase-free penicillin amidase from Alcaligenes sp. active against various beta-lactams was purified to homogeneity. The enzyme can hydrolyze penicillin G to 6-amino penicillanic acid (6-APA) and furnish penicillin G from 6-APA and phenyl acetic acid by condensation. The penicillin amidase is a heterodimer o...

journal_title：Applied microbiology and biotechnology

authors： Das S,Gayen JR,Pal A,Ghosh K,Rosazza JP,Samanta TB

更新日期：2004-08-01 00:00:00

abstract：:Potato pulp, one of the agricultural waste products obtained in high quantities during starch production, contains starch, cellulose, hemicelluloses, pectin, proteins, free amino acids and salts. It exhibits physical and physicochemical properties of a typical colloid. It is mainly used, in a dried and pelleted form, ...

journal_title：Applied microbiology and biotechnology

authors： Mayer F,Hillebrandt JO

更新日期：1997-10-01 00:00:00

abstract：:A simple structured model for the dynamics of phosphate-limited batch fermentations with Streptomyces tendae is presented. The model describes the influence of intracellular phosphate storage upon the growth behav our of the culture. The development of the model takes into account the possible internal regulatory proc...

journal_title：Applied microbiology and biotechnology

authors： Mundry C,Kuhn KP

更新日期：1991-06-01 00:00:00

abstract：:Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were...

journal_title：Applied microbiology and biotechnology

authors： Zheng Y,Yang W,Sun X,Wang SP,Rui YC,Luo CY,Guo LD

更新日期：2012-03-01 00:00:00

abstract：:This study aimed to evaluate the effects of partial reducing rumen-protected Lys (RPLys) on rumen fermentation and microbial composition in heifers. Three ruminal fistulated Holstein Friesian bulls were used to determine the effective degradability of RPLys using an in situ method at incubation times of 0, 2, 6, 12, 1...

journal_title：Applied microbiology and biotechnology

authors： Kong F,Gao Y,Tang M,Fu T,Diao Q,Bi Y,Tu Y

更新日期：2020-08-01 00:00:00

abstract：:Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in the environment is often limited due to unfavorable nutrient conditions for the bacteria that use these PAHs as sole source of carbon and energy. Mycobacterium and Sphingomonas are 2 PAH-degrading specialists commonly present in PAH-polluted soil, but not mu...

journal_title：Applied microbiology and biotechnology

authors： Leys NM,Bastiaens L,Verstraete W,Springael D

更新日期：2005-03-01 00:00:00

abstract：:A microbial peptide amidase was found in a limited screening and purified about 500-fold from Stenotrophomonas maltophilia. The native enzyme has a molecular mass of 38 kDa (gel filtration). The sequence of the first 16 amino acids was determined by Edman degradation. The isoelectric point was found to be around 5.8. ...

journal_title：Applied microbiology and biotechnology

authors： Stelkes-Ritter U,Wyzgol K,Kula MR

更新日期：1995-12-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:The present review describes the possibility of in vitro meat production with the help of advanced co-culturing methods. In vitro meat production method could be a possible alternative for the conventional meat production. Originally, the research on in vitro meat production was initiated by the National Aeronautics a...

journal_title：Applied microbiology and biotechnology

authors： Pandurangan M,Kim DH

更新日期：2015-07-01 00:00:00

abstract：:Biochemical processes in biogas plants are still not fully understood. Especially, the identification of possible bottlenecks in the complex fermentation processes during biogas production might provide potential to increase the performance of biogas plants. To shed light on the question which group of organism consti...

journal_title：Applied microbiology and biotechnology

authors： Refai S,Wassmann K,Deppenmeier U

更新日期：2014-08-01 00:00:00

abstract：:The molecular mechanisms active during the endophytic phase of the fungus Pochonia chlamydosporia are still poorly understood. In particular, few data are available on the links between the endophyte and the root response, as modulated by noncoding small RNAs. In this study, we describe the microRNAs (miRNAs) that are...

journal_title：Applied microbiology and biotechnology

authors： Pentimone I,Lebrón R,Hackenberg M,Rosso LC,Colagiero M,Nigro F,Ciancio A

更新日期：2018-01-01 00:00:00

abstract：:This research develops a safe, inexpensive, and more accessible source for synthesis of silver nanoparticles. The bioactive silver composites synthesized by Lactococcus lactis 56 KY484989 (LCLB56-AgCs) were characterized by various physico-chemical techniques and investigated for their antimicrobial activity and cytot...

journal_title：Applied microbiology and biotechnology

authors： Viorica RP,Pawel P,Kinga M,Michal Z,Katarzyna R,Boguslaw B

更新日期：2017-10-01 00:00:00

abstract：:One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique ...

journal_title：Applied microbiology and biotechnology

authors： Landgrebe D,Haake C,Höpfner T,Beutel S,Hitzmann B,Scheper T,Rhiel M,Reardon KF

更新日期：2010-09-01 00:00:00

abstract：:Extremophilic microorganisms are adapted to survive in ecological niches such as at high temperatures, extremes of pH, high salt concentrations and high pressure. These microorganisms produce unique biocatalysts that function under extreme conditions comparable to those prevailing in various industrial processes. Some...

journal_title：Applied microbiology and biotechnology

authors： Niehaus F,Bertoldo C,Kähler M,Antranikian G

更新日期：1999-06-01 00:00:00

abstract：:Bacterial cells are known to adapt to challenging environmental conditions such as osmotic stress. However, most of the work done in this field describes the adaptation of growing populations where the new generations acquire traits that improve their ability to survive. In the present study, the responses of Rhodococ...

journal_title：Applied microbiology and biotechnology

authors： de Carvalho CC,Marques MP,Hachicho N,Heipieper HJ

更新日期：2014-06-01 00:00:00

abstract：:Despite the known hazardous effects of antimony (Sb) on human health, Sb monitoring biosensors have not been as actively investigated as arsenic (As) biosensors. Whole-cell bioreporters (WCBs) employing an arsenic-responsive operon and a regulatory protein (ArsR) are reportedly capable of monitoring arsenite, arsenate...

journal_title：Applied microbiology and biotechnology

authors： Lee W,Kim H,Jang G,Kim BG,Yoon Y

更新日期：2020-03-01 00:00:00

abstract：:Pichia pastoris is widely used as a host system for heterologous protein expression in both academia and industry. Production is typically accomplished by a fed-batch induction process that is known to have negative impacts on cell physiology that impose limits on both protein yields and quality. We have analysed reco...

journal_title：Applied microbiology and biotechnology

authors： Hesketh AR,Castrillo JI,Sawyer T,Archer DB,Oliver SG

更新日期：2013-11-01 00:00:00

abstract：:A cytochrome P450 and an iron-sulfur protein, whose expression was specifically induced during growth of Mycobacterium sp. strain HE5 on morpholine as the sole source of carbon, nitrogen, and energy were purified to apparent homogeneity. Due to the lack of enzymatic activity, carbon monoxide difference spectra and det...

journal_title：Applied microbiology and biotechnology

authors： Sielaff B,Andreesen JR,Schräder T

更新日期：2001-08-01 00:00:00

abstract：:We are beginning to see how the microbiota of the human gastrointestinal tract (GIT) can drive the development of new products to benefit human health and wellbeing. Despite the growing market for prebiotics and probiotics, there are currently no commercial products available that aid or increase the attachment of hea...

journal_title：Applied microbiology and biotechnology

authors： Morrin ST,Hickey RM

更新日期：2020-02-01 00:00:00

abstract：:A cDNA encoding a bifunctional acetylxylan esterase/xylanase, XynS20E, was cloned from the ruminal fungus Neocallimastix patriciarum. A putative conserved domain of carbohydrate esterase family 1 was observed at the N-terminus and a putative conserved domain of glycosyl hydrolase family 11 was detected at the C-termin...

journal_title：Applied microbiology and biotechnology

authors： Pai CK,Wu ZY,Chen MJ,Zeng YF,Chen JW,Duan CH,Li ML,Liu JR

更新日期：2010-02-01 00:00:00

abstract：:Flow cytometry is a rapid and quantitative method to determine bacterial viability. Although different stains can be used to establish viability, staining protocols are inconsistent and lack a general optimization approach. Very few "true" multicolor protocols, where dyes are combined in one sample, have been develope...

journal_title：Applied microbiology and biotechnology

authors： Buysschaert B,Byloos B,Leys N,Van Houdt R,Boon N

更新日期：2016-11-01 00:00:00

abstract：:α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone. We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergi...

journal_title：Applied microbiology and biotechnology

authors： Ishikawa M,Kawasaki M,Shiono Y,Koseki T

更新日期：2018-04-01 00:00:00

abstract：:The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. ...

journal_title：Applied microbiology and biotechnology

authors： Nguyen TKM,Ki MR,Son RG,Pack SP

更新日期：2019-03-01 00:00:00

abstract：:Cyanophycin is non-ribosomally synthesized protein-like copolymer. Synthesis of cyanophycin is catalyzed by cyanophycin synthetase (CphA). In this study, a novel cyanophycin synthetase CphA49 belonging to NOR5 clade of Gammaproteobacteria was identified with primer-based screening from a deep-sea sediment metagenomic ...

journal_title：Applied microbiology and biotechnology

authors： Du J,Li L,Ding X,Hu H,Lu Y,Zhou S

更新日期：2013-10-01 00:00:00

abstract：:Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...

journal_title：Applied microbiology and biotechnology

authors： Zhang Q,Wu ZM,Hao CL,Tang XL,Zheng RC,Zheng YG

更新日期：2019-07-01 00:00:00

abstract：:Agrobacterium tumefaciens has been widely used as a tool for transgenesis in plants. The availability of its genome sequence should facilitate the directed engineering of improved properties; however, the current genome engineering options are laborious. Here, we investigated whether the lambda Red operon can be appli...

journal_title：Applied microbiology and biotechnology

authors： Hu S,Fu J,Huang F,Ding X,Stewart AF,Xia L,Zhang Y

更新日期：2014-03-01 00:00:00

abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

journal_title：Applied microbiology and biotechnology

authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

更新日期：2004-05-01 00:00:00