Laccase-catalysed synthesis of coupling products of phenolic substrates in different reactors.

abstract：:AmyC, a glycoside hydrolase family 57 (GH57) enzyme of Thermotoga maritima MSB8, has previously been identified as an intracellular α-amylase playing a role in either maltodextrin utilization or storage polysaccharide metabolism. However, the α-amylase specificity of AmyC is questionable as extensive phylogenetic anal...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Leemhuis H,Janeček Š,Martinovičová M,Pijning T,van der Maarel MJEC

更新日期：2019-08-01 00:00:00

abstract：:Vanillin can be produced on a commercial scale by depolymerising renewable lignin. One product of microbial metabolism of vanillin by common soil microbes, such as Acinetobacter baylyi, is a tricarboxylic acid with a butadiene backbone known as 3-carboxy muconate (3CM). Three enzymes, 4-hydroxy benzaldehyde dehydrogen...

journal_title：Applied microbiology and biotechnology

authors： Gosling A,Fowler SJ,O'Shea MS,Straffon M,Dumsday G,Zachariou M

更新日期：2011-04-01 00:00:00

abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Vassileva M,Nikolaeva I

更新日期：2006-06-01 00:00:00

abstract：:A metabolic heat-based model was used for estimating the growth of Kluyveromyces marxianus, and the modified Luedeking-Piret kinetic model was used for describing the inulinase production kinetics. For the first time, a relationship was developed to relate inulinase production kinetics directly to metabolic heat gener...

journal_title：Applied microbiology and biotechnology

authors： Santharam L,Samuthirapandi AB,Easwaran SN,Mahadevan S

更新日期：2017-03-01 00:00:00

abstract：:A newly isolated Aspergillus niger strain containing epoxide hydrolase was used to resolve racemic glycidyl azide and four derivatives to the (R)-enantiomers. After optimization of the biotransformation conditions, (R)-glycidyl azide was produced with good enantioselectivity (e.e.s > 95 %, E > 20). The substrate struc...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Shen H,Wei C,Zhu Q

更新日期：2013-03-01 00:00:00

abstract：:For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The su...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Xie Y,Yu Z,Meng G,Wu Z

更新日期：2019-10-01 00:00:00

abstract：:An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temper...

journal_title：Applied microbiology and biotechnology

authors： Singh RK,Tiwari MK,Kim D,Kang YC,Ramachandran P,Lee JK

更新日期：2013-08-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been modified successfully for production of numerous metabolites and therapeutic proteins through metabolic engineering, but has not been utilized to date for the production of lipid-derived compounds. We developed a lipid metabolic engineering strategy in S. cerevisiae based up...

journal_title：Applied microbiology and biotechnology

authors： Dyer JM,Chapital DC,Kuan JW,Mullen RT,Pepperman AB

更新日期：2002-07-01 00:00:00

abstract：:Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. Th...

journal_title：Applied microbiology and biotechnology

authors： Deng L,Zeng G,Fan C,Lu L,Chen X,Chen M,Wu H,He X,He Y

更新日期：2015-10-01 00:00:00

abstract：:Tacrolimus (FK506), an effective immunosuppressant, is widely used in the treatment of autoimmune diseases. In this study, we identified that BulZ, a Streptomyces antibiotic regulatory protein (SARP) family regulator, acted as a positive regulator for spore differentiation and tacrolimus production. A knockout of bulZ...

journal_title：Applied microbiology and biotechnology

authors： Ma D,Wang C,Chen H,Wen J

更新日期：2018-06-01 00:00:00

abstract：:Even though salbutamol (SAL) had remarkable effects on the enhancement of growth rate and carcass composition in different livestock species such as cattle, pigs, sheep and poultry, it was banned as a growth promoter because of its adverse effects on health. However, the specific mechanism by which salbutamol enhances...

journal_title：Applied microbiology and biotechnology

authors： Lu C,Zhou J,Li Y,Zhang D,Wang Z,Li Y,Cheong L,Zhang C,Su X

更新日期：2017-07-01 00:00:00

abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

journal_title：Applied microbiology and biotechnology

authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

更新日期：2012-01-01 00:00:00

abstract：:The hemiascomycete Ashbya gossypii develops a mycelium. Nutritional stress leads to its differentiation into sporangia. These generate spores. In parallel, the yellow pigment riboflavin is produced. Intracellularly accumulated riboflavin, made visible as a bright green fluorescence, was observed in only 60% of the hyp...

journal_title：Applied microbiology and biotechnology

authors： Nieland S,Stahmann KP

更新日期：2013-12-01 00:00:00

abstract：:Streptolydigin, a secondary metabolite produced by Streptomyces lydicus, is a potent inhibitor of bacterial RNA polymerases. It has been suggested that streptolydigin biosynthesis is associated with polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS). Thus, there is great interest in understanding the...

journal_title：Applied microbiology and biotechnology

authors： Zhao GR,Luo T,Zhou YJ,Jiang X,Qiao B,Yu FM,Yuan YJ

更新日期：2009-05-01 00:00:00

abstract：:Cloning and biochemical characterization of a novel extracellular medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase from Streptomyces exfoliatus K10 DSMZ 41693 are described. The primary structure of the depolymerase (PhaZSex2) includes the lipase consensus sequence (serine-histidine-aspartic acid) which...

journal_title：Applied microbiology and biotechnology

authors： Martínez V,de Santos PG,García-Hidalgo J,Hormigo D,Prieto MA,Arroyo M,de la Mata I

更新日期：2015-11-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:The rapid global urbanization and other extensive anthropogenic activities exacerbated the worldwide human health risks induced by antibiotic resistance genes (ARGs). Knowledge of the origins and dissemination of ARGs is essential for understanding modern resistome, while little information is known regarding the over...

journal_title：Applied microbiology and biotechnology

authors： Ouyang WY,Huang FY,Zhao Y,Li H,Su JQ

更新日期：2015-07-01 00:00:00

abstract：:Prenyltransferase NovQ is a vital class involved in the biosynthesis of secondary metabolites such as clorobiocin and novobiocin. To investigate the relationship between structure and catalytic properties of NovQ, here, we have analyzed the substrate-binding site, namely PT barrel, and revealed that menadione hydroqui...

journal_title：Applied microbiology and biotechnology

authors： Ni W,Zheng Z,Liu H,Wang P,Wang H,Sun X,Yang Q,Fang Z,Tang H,Zhao G

更新日期：2020-05-01 00:00:00

abstract：:The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the ...

journal_title：Applied microbiology and biotechnology

authors： Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:The temporal and spatial distribution of the genus Bacillus and Clostridium histolyticum group in swine manure composting was determined by fluorescent in situ hybridization using fluorescently labeled 16S rRNA-targeted oligonucleotide probes LGC353b and Chis150, respectively. The temporal distribution of total bacter...

journal_title：Applied microbiology and biotechnology

authors： Yi J,Zheng R,Li F,Chao Z,Deng CY,Wu J

更新日期：2012-03-01 00:00:00

abstract：INTRODUCTION:Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase. M...

journal_title：Applied microbiology and biotechnology

authors： Kroll J,Klinter S,Steinbüchel A

更新日期：2011-02-01 00:00:00

abstract：:Microsomal fraction of fungal cells grabs the attention of many researchers for it contains enzymes that play a role in biotechnologically relevant processes. Microsomal enzymes, namely, CYP450s, were shown to metabolize a wide range of xenobiotic compounds, including PAHs, PCBs, dioxins, and endocrine disruptors, and...

journal_title：Applied microbiology and biotechnology

authors： Svobodová K,Mikesková H,Petráčková D

更新日期：2013-12-01 00:00:00

abstract：:The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS(-) (modified MRS - omitting glucose, acetate and Tween 80) and distilled water (H(2)O). In MRS(-), addition ...

journal_title：Applied microbiology and biotechnology

authors： Lüthi-Peng Q,Dileme FB,Puhan Z

更新日期：2002-07-01 00:00:00

abstract：:The engineered Saccharomyces cerevisiae strain △faa1△faa4 [Acot5s] was demonstrated to accumulate more free fatty acids (FFA) previously. Here, comparative proteomic analysis was performed to get a global overview of metabolic regulation in the strain. Over 500 proteins were identified, and 82 of those proteins were f...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lee JJL,Zhang J,Chen WN

更新日期：2016-02-01 00:00:00

abstract：:The effects of three heavy metals (Co, Ni and Cd) on the growth kinetics of five bacterial strains with different characteristics (Pseudomonas sp., Phaeobacter sp. strain 27-4, Listonella anguillarum, Carnobacterium piscicola and Leuconostoc mesenteroides subsp. lysis) were studied in a batch system. A bivariate model...

journal_title：Applied microbiology and biotechnology

authors： Rial D,Vázquez JA,Murado MA

更新日期：2011-05-01 00:00:00

abstract：:The genetic improvement of winemaking yeasts is a virtually infinite process, as the design of new strains must always cope with varied and ever-evolving production contexts. Good wine yeasts must feature both good primary traits, which are related to the overall fermentative fitness of the strain, and secondary trait...

journal_title：Applied microbiology and biotechnology

authors： Bonciani T,De Vero L,Mezzetti F,Fay JC,Giudici P

更新日期：2018-03-01 00:00:00

abstract：:This study focused on the use the thermophilic anaerobic granulae in the start-up of 70 degrees C acetate-fed upflow anaerobic sludge-blanket (UASB) reactors and the kinetics of granulae grown at 70 degrees C. In the UASB reactors, chemical oxygen demand removal commenced within 48 h of the start-up. The maximum reduc...

journal_title：Applied microbiology and biotechnology

authors： Lepistö R,Rintala JA

更新日期：1995-11-01 00:00:00

abstract：:Dyes and dyestuffs find use in a wide range of industries but are of primary importance to textile manufacturing. Wastewater from the textile industry can contain a variety of polluting substances including dyes. Increasingly, environmental legislation is being imposed to control the release of dyes, in particular azo...

journal_title：Applied microbiology and biotechnology

authors： McMullan G,Meehan C,Conneely A,Kirby N,Robinson T,Nigam P,Banat IM,Marchant R,Smyth WF

更新日期：2001-07-01 00:00:00

abstract：:Recent progress in enzymatic (R)-phenylacetylcarbinol (PAC) production has established the need for low cost and efficient biocatalyst preparation. Pyruvate decarboxylase (PDC) added in the form of Candida utilis cells showed higher stability towards benzaldehyde and temperature in comparison with partially purified p...

journal_title：Applied microbiology and biotechnology

authors： Satianegara G,Breuer M,Hauer B,Rogers PL,Rosche B

更新日期：2006-03-01 00:00:00

abstract：:Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

journal_title：Applied microbiology and biotechnology

authors： McIntyre DA,Harlander SK

更新日期：1993-11-01 00:00:00