Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH.

abstract：:Quorum sensing (QS) regulates virulence and biofilm formation in Pseudomonas aeruginosa and other medically relevant bacteria. Human paraoxonases (hPONs) are a family of closely related enzymes with multiple functions, including inactivation of the QS signal molecule in P. aeruginosa. However, there is no direct evide...

journal_title：Applied microbiology and biotechnology

authors： Ma F,Wang Y,Zhang Y,Xiong N,Yang B,Chen S

更新日期：2009-05-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After th...

journal_title：Applied microbiology and biotechnology

authors： An C,Kuda T,Yazaki T,Takahashi H,Kimura B

更新日期：2014-05-01 00:00:00

abstract：:A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...

journal_title：Applied microbiology and biotechnology

authors： Park HY,Kim M,Han J

更新日期：2011-08-01 00:00:00

abstract：:The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an optimal C. glutamicum genome, a precise site-directed gene integration method was developed by using a pair of mutant lox sites, one a right element (RE) mutant lox site and the other a left element (LE)...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Inui M,Yukawa H

更新日期：2007-12-01 00:00:00

abstract：:Twenty-three genotypes of maitake (Grifola frondosa) from Asia, North America and Europe were compared for biological efficiency (BE), quality and crop cycle time. Significant differences among lines were found for BE and crop cycle time when mushrooms were produced on nutrient-supplemented (15% millet, 10% wheat bran...

journal_title：Applied microbiology and biotechnology

authors： Shen Q,Royse DJ

更新日期：2002-02-01 00:00:00

abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

journal_title：Applied microbiology and biotechnology

authors： Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

更新日期：2015-07-01 00:00:00

abstract：:Benzene dioxygenase (BDO; EC 1.14.12.3) from Pseudomonas putida ML2 dihydroxylates benzene to produce cis-1,2-dihydroxy-cyclohexa-3,5-diene. As well as oxidising benzene and toluene, cell-free extracts of Escherichia coli JM109 expressing recombinant BDO oxidised cyclohexene, 1-methylcyclohexene and 3-methylcyclohexen...

journal_title：Applied microbiology and biotechnology

authors： Swift RJ,Carter SF,Widdowson DA,Mason JR,Leak DJ

更新日期：2001-06-01 00:00:00

abstract：:Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

更新日期：2015-11-01 00:00:00

abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

journal_title：Applied microbiology and biotechnology

authors： Alonso S,Rendueles M,Díaz M

更新日期：2012-12-01 00:00:00

abstract：:Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to delta7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Delta7-desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofact...

journal_title：Applied microbiology and biotechnology

authors： Valcarce G,Florin-Christensen J,Nudel C

更新日期：2000-05-01 00:00:00

abstract：:Substrate oxidation of aromatic substances by the enzyme laccase followed by a heteromolecular coupling with a co-substrate is a promising possibility for the synthesis of new compounds. To find a suitable reactor for the effective production of new compounds, the laccase-catalysed coupling of 3-(3,4-dihydroxyphenyl)p...

journal_title：Applied microbiology and biotechnology

authors： Pilz R,Hammer E,Schauer F,Kragl U

更新日期：2003-02-01 00:00:00

abstract：:A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules throug...

journal_title：Applied microbiology and biotechnology

authors： Moldes C,Farinós GP,de Eugenio LI,García P,García JL,Ortego F,Hernández-Crespo P,Castañera P,Prieto MA

更新日期：2006-08-01 00:00:00

abstract：:Bioconversion of lignocellulosic biomass to high-value bioproducts by fermentative microorganisms has drawn extensive attentions worldwide. Lignocellulosic biomass cannot be efficiently utilized by microorganisms, such as Saccharomyces cerevisiae, but has to be pretreated prior to fermentation. Aldehyde compounds, as ...

journal_title：Applied microbiology and biotechnology

authors： Wang H,Li Q,Kuang X,Xiao D,Han X,Hu X,Li X,Ma M

更新日期：2018-12-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:Paddy fields are a significant source of methane and contribute up to 20% of total methane emissions from wetland ecosystems. These inundated, anoxic soils featuring abundant nitrogen compounds and methane are an ideal niche for nitrate-dependent anaerobic methanotrophs. After 2 years of enrichment with a continuous s...

journal_title：Applied microbiology and biotechnology

authors： Vaksmaa A,Guerrero-Cruz S,van Alen TA,Cremers G,Ettwig KF,Lüke C,Jetten MSM

更新日期：2017-09-01 00:00:00

abstract：:The quorum sensing signalling molecules 2-heptyl-3-hydroxy-4(1H)-quinolone, termed the "Pseudomonas quinolone signal" (PQS), and 2-heptyl-4(1H)-quinolone (HHQ) play an important role in the control of virulence gene expression in Pseudomonas aeruginosa. To construct a bioreporter for the specific and sensitive detecti...

journal_title：Applied microbiology and biotechnology

authors： Müller C,Fetzner S

更新日期：2013-01-01 00:00:00

abstract：:A family I cellulose-binding domain (CBD) and a serine- and threonine-rich linker peptide were cloned from the fungi Aspergillus japonicus and Aspergillus aculeatus. A glutathione S-transferase (GST) fusion protein comprising GST and a peptide linker with the CBD fused to its C-terminus, was expressed in Escherichia c...

journal_title：Applied microbiology and biotechnology

authors： Quentin M,Ebbelaar M,Derksen J,Mariani C,van Der Valk H

更新日期：2002-04-01 00:00:00

abstract：:The ability to produce (R)- or (S)-beta-phenylalanine ethyl ester (3-amino-3-phenylpropionic acid ethyl ester, BPAE) from racemic BPAE through stereoselective hydrolysis was screened for in BPAE-assimilating microorganisms. Sphingobacterium sp. 238C5 and Arthrobacter sp. 219D2 were found to be potential catalysts for ...

journal_title：Applied microbiology and biotechnology

authors： Ogawa J,Mano J,Shimizu S

更新日期：2006-05-01 00:00:00

abstract：:Regulatable promoters are important genetic tools, particularly for assigning function to essential and redundant genes. They can also be used to control the expression of enzymes that influence metabolic flux or protein secretion, thereby optimizing product yield in bioindustry. This review will focus on regulatable ...

journal_title：Applied microbiology and biotechnology

authors： Fuller KK,Dunlap JC,Loros JJ

更新日期：2018-05-01 00:00:00

abstract：:Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

journal_title：Applied microbiology and biotechnology

authors： Dillon AJ,Zorgi C,Camassola M,Henriques JA

更新日期：2006-05-01 00:00:00

abstract：:Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as beta-galactosidase activity. Transcription of the crystal protein gene fusion began im...

journal_title：Applied microbiology and biotechnology

authors： Ahmed HK,Mitchell WJ,Priest FG

更新日期：1995-05-01 00:00:00

abstract：:The dynamics of bacterial communities play an important role in solid-state fermentation (SSF). Poly-γ-glutamic acid (γ-PGA) was produced by Bacillus amyloliquefaciens C1 in SSF using dairy manure compost and monosodium glutamate production residuals as basic substrates. The production of γ-PGA reached a maximum of 0....

journal_title：Applied microbiology and biotechnology

authors： Yong X,Cui Y,Chen L,Ran W,Shen Q,Yang X

更新日期：2011-11-01 00:00:00

abstract：:A novel biosuperabsorbent protein hydrogel was prepared from protein-rich alcoholic-alkali soluble parts of zygomycete Rhizomucor pusillus biomass. The fungal protein content was 46.8%, and the lipid content was 13.1%. Extraction of protein from this microorganism through the method applied prevents protein decomposit...

journal_title：Applied microbiology and biotechnology

authors： Majdejabbari S,Barghi H,Taherzadeh MJ

更新日期：2011-12-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00

abstract：:Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from ...

journal_title：Applied microbiology and biotechnology

authors： Leite HA,Silva AB,Gomes FP,Gramacho KP,Faria JC,de Souza JT,Loguercio LL

更新日期：2013-03-01 00:00:00

abstract：:The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the r...

journal_title：Applied microbiology and biotechnology

authors： Dong Y,Geng J,Liu J,Pang M,Awan F,Lu C,Liu Y

更新日期：2019-05-01 00:00:00

abstract：:Polyunsaturated fatty acids (PUFAs) are essential lipids for cell function, normal growth, and development, serving as key structural components of biological membranes and modulating critical signal transduction events. Omega-3 (n-3) long chain PUFAs (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic ...

journal_title：Applied microbiology and biotechnology

authors： Wang M,Chen H,Ailati A,Chen W,Chilton FH,Todd Lowther W,Chen YQ

更新日期：2018-01-01 00:00:00

abstract：:Corynebacterium glutamicum shows a great potential for the production of the polyamide monomer putrescine (1,4-diaminobutane). Previously, we constructed the putrescine-producing strain PUT1 by deletion of argF, the gene for ornithine transcarbamoylase (OTC), and argR, encoding the L-arginine repressor, combined with ...

journal_title：Applied microbiology and biotechnology

authors： Schneider J,Eberhardt D,Wendisch VF

更新日期：2012-07-01 00:00:00

abstract：:Expression of recombinant proteins in Escherichia coli often requires use of inducible promoters to shorten the lag phase and improve protein productivity and final protein titer. Synthetic molecules that cannot be metabolized by E. coli, such as isopropyl thiogalactopyranoside (IPTG), have been frequently used to tri...

journal_title：Applied microbiology and biotechnology

authors： Anilionyte O,Liang H,Ma X,Yang L,Zhou K

更新日期：2018-08-01 00:00:00