Double Candida antarctica lipase B co-display on Pichia pastoris cell surface based on a self-processing foot-and-mouth disease virus 2A peptide.

abstract：:A looming global energy crisis has directly increased biomethanation processes using anaerobic digestion technology. However, much knowledge on the microbial community structure, their distribution within the digester and related functions remains extremely scanty and unavailable in some cases, yet very valuable in th...

journal_title：Applied microbiology and biotechnology

authors： Ambuchi JJ,Liu J,Wang H,Shan L,Zhou X,Mohammed MO,Feng Y

更新日期：2016-05-01 00:00:00

abstract：:Silver nanoparticles (AgNPs) have received tremendous attention due to their significant antimicrobial properties. Large numbers of reports are available on the physical, chemical, and biological syntheses of colloidal AgNPs. Since there is a great need to develop ecofriendly and sustainable methods, biological system...

journal_title：Applied microbiology and biotechnology

authors： Singh R,Shedbalkar UU,Wadhwani SA,Chopade BA

更新日期：2015-06-01 00:00:00

abstract：:meta-Cleavage product (MCP) hydrolases can catalyze relatively low reactive carbon-carbon bond hydrolysis of products, which are derived from the meta-cleavage of catechols. The strict substrate selectivity of MCP hydrolases attracts an interest to understand the determinants of substrate specificity. Compared with co...

journal_title：Applied microbiology and biotechnology

authors： Zhou H,Qu Y,Shen E,Kong C,Zhang X,Ma Q,Zhou J

更新日期：2013-06-01 00:00:00

abstract：:Listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. In this study, we investigated L. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species o...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Zhang H,Wu C,Deng W,Wang D,Zhao G,Song J,Jiang Y

更新日期：2016-04-01 00:00:00

abstract：:Human interleukin 6 (hIL-6) overproduced in Escherichia coli HB101 was found to partially retain the initiator methionine (Met) residue (Met-hIL-6). In order to remove the residual N-terminal Met in vivo, an attempt was made to express hIL-6 in aminopeptidase-P (Ap-P)-hyperproducing strains, since the N-terminus Met-P...

journal_title：Applied microbiology and biotechnology

authors： Yasueda H,Kikuchi Y,Kojima H,Nagase K

更新日期：1991-11-01 00:00:00

abstract：:Cordyceps spp. is the herbal medication initially used in China and has been reported as the unique resource of cordycepin. Cordycepin exhibits many health benefits, including anti-photoaging and anti-pigmentation; therefore, it potentially is a bioactive ingredient of cosmetic products. In order to enrich cordycepin ...

journal_title：Applied microbiology and biotechnology

authors： Kunhorm P,Chaicharoenaudomrung N,Noisa P

更新日期：2019-02-01 00:00:00

abstract：:Dengue virus (DENV) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses. Together, they caused most arthropod-borne diseases in the world. Previously, we had demonstrated that both live attenuated and inactivated JE vaccines elicited cross-protection against DENV infection, and a DNA ...

journal_title：Applied microbiology and biotechnology

authors： Gao N,Li J,Sheng Z,Chen H,Fan D,Wang P,An J

更新日期：2019-06-01 00:00:00

abstract：:Panax ginseng C.A. Meyer (ginseng) is a well-known medicinal plant that has been traditionally used in the oriental countries for centuries. Wild ginseng is a scarce and rare commodity. Field cultivation of the ginseng plant is a time-consuming and labor-intensive process. Ginsenosides, a group of glycosylated triterp...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Georgiev MI,Kim YS,Jeong CS,Kim SJ,Park SY,Paek KY

更新日期：2014-01-01 00:00:00

abstract：:A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

journal_title：Applied microbiology and biotechnology

authors： Melin ES,Ferguson JF,Puhakka JA

更新日期：1997-06-01 00:00:00

abstract：:Five types of pharmaceuticals and personal care products (PPCPs) substances were selected as pollutants in this study. The effects of the removal of these pollutants and the microbial succession process in a granular sludge membrane bioreactor (GMBR) were investigated. Results showed that wastewater containing PPCPs i...

journal_title：Applied microbiology and biotechnology

authors： Wang XC,Shen JM,Chen ZL,Zhao X,Xu H

更新日期：2016-09-01 00:00:00

abstract：:Severe dengue is more likely found during secondary heterologous dengue virus (DENV) infection or primary infection of infants born to dengue-immune mothers and led to the hypothesis of antibody-dependent enhancement (ADE). It has been reported that pre-membrane (prM)-reactive antibodies do not efficiently neutralize ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Si L,Luo Y,Guo X,Zhou J,Fang D,Yan H,Zeng G,Jiang L

更新日期：2015-11-01 00:00:00

abstract：:Sulfide detoxification can be catalyzed by ancient membrane-bound flavoproteins, sulfide:quinone oxidoreductases (Sqr), which have important roles in sulfide homeostasis and sulfide-dependent energy conservation processes by transferring electrons from sulfide to respiratory or photosynthetic membrane electron flow. S...

journal_title：Applied microbiology and biotechnology

authors： Duzs Á,Tóth A,Németh B,Balogh T,Kós PB,Rákhely G

更新日期：2018-06-01 00:00:00

abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...

journal_title：Applied microbiology and biotechnology

authors： Sakai T,Nakamura N,Umitsuki G,Nagai K,Wachi M

更新日期：2007-08-01 00:00:00

abstract：:Anaerobic digestion treatments have often been used for biological stabilization of solid wastes. These treatment processes generate biogas which can be used as a renewable energy sources. Recently, anaerobic digestion of solid wastes has attracted more interest because of current environmental problems, most especial...

journal_title：Applied microbiology and biotechnology

authors： Muhammad Nasir I,Mohd Ghazi TI,Omar R

更新日期：2012-07-01 00:00:00

abstract：:Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

journal_title：Applied microbiology and biotechnology

authors： Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

更新日期：2013-08-01 00:00:00

abstract：:Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...

journal_title：Applied microbiology and biotechnology

authors： Zhang Q,Wu ZM,Hao CL,Tang XL,Zheng RC,Zheng YG

更新日期：2019-07-01 00:00:00

abstract：:Despite being a yellow pigment visible to the human eye, coelimycin (CPK) remained to be an undiscovered secondary metabolite for over 50 years of Streptomyces research. Although the function of this polyketide is still unclear, we now know that its "cryptic" nature is attributed to a very complex and precise mechanis...

journal_title：Applied microbiology and biotechnology

authors： Bednarz B,Kotowska M,Pawlik KJ

更新日期：2019-08-01 00:00:00

abstract：:Mannosylerythritol lipids (MEL), which are abundantly secreted by yeasts, are one of the most promising biosurfactants known. To obtain various types of MEL and to attain a broad range of applications for them, screening of novel producers was undertaken. Thirteen strains of yeasts were successfully isolated as potent...

journal_title：Applied microbiology and biotechnology

authors： Konishi M,Morita T,Fukuoka T,Imura T,Kakugawa K,Kitamoto D

更新日期：2007-06-01 00:00:00

abstract：:Influence of pressure (P) and oxygen partial pressure (PO2) on cultivation of various Streptomyces spp. and Micromonospora purpurea was examined in pressurized air-lift and stirred tank fermenters. The maximum PO2 was 2100 mbar. Growth and product formation of all cultures tested were markedly influenced by PO2 higher...

journal_title：Applied microbiology and biotechnology

authors： Liefke E,Kaiser D,Onken U

更新日期：1990-03-01 00:00:00

abstract：:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and ...

journal_title：Applied microbiology and biotechnology

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2014-03-01 00:00:00

abstract：:The pyrene-degrading Mycobacterium strain AP1 was found to utilize fluoranthene as a sole source of carbon and energy. Identification of metabolites formed from fluoranthene (by growing cells and washed-cell suspensions), the kinetics of metabolite accumulation, and metabolite-feeding studies all indicated that strain...

journal_title：Applied microbiology and biotechnology

authors： López Z,Vila J,Minguillón C,Grifoll M

更新日期：2006-05-01 00:00:00

abstract：:Drought, high/low temperature, and salinity are abiotic stress factors accepted as the main reason for crop yield losses in a world with growing population and food price increases. Additional problems create nutrient limitations and particularly low P soil status. The problem of phosphate fertilizers, P plant nutriti...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Eichler-Löbermann B,Vassileva M

更新日期：2012-08-01 00:00:00

abstract：:A laccase from Pycnoporus sanguineus was purified by two steps using phenyl-Sepharose columm. A typical procedure provided 54.1-fold purification, with a yield of 8.37%, using syringaldazine as substrate. The molecular weight of the purified laccase was 69 and 68 kDa as estimated by 12% (w/v) SDS-PAGE gel and by gel f...

journal_title：Applied microbiology and biotechnology

authors： Garcia TA,Santiago MF,Ulhoa CJ

更新日期：2007-05-01 00:00:00

abstract：:Optimization of yields and productivities in reductive whole-cell biotransformations is an important issue for the industrial application of such processes. In a recent study with Escherichia coli, we analyzed the reduction of the prochiral β-ketoester methyl acetoacetate by an R-specific alcohol dehydrogenase (ADH) t...

journal_title：Applied microbiology and biotechnology

authors： Siedler S,Bringer S,Blank LM,Bott M

更新日期：2012-02-01 00:00:00

abstract：:Streptokinase, a therapeutically important thrombolytic agent, is prone to C-terminal degradation and plasmin-mediated proteolytic processing. Since the protein was glycosylated during secretion from Pichia pastoris, therefore, the role of carbohydrate moieties on its stability was analyzed via in vivo blocking of N-g...

journal_title：Applied microbiology and biotechnology

authors： Adivitiya,Babbal,Mohanty S,Khasa YP

更新日期：2018-12-01 00:00:00

abstract：:Empirical approaches to discover anticancer drugs and cancer treatments have made limited progress in the past several decades in finding a cure for cancer. The expanded knowledge of the molecular basis of tumorigenesis and metastasis, together with the inherently vast structural diversity of natural compounds found i...

journal_title：Applied microbiology and biotechnology

authors： Zaidman BZ,Yassin M,Mahajna J,Wasser SP

更新日期：2005-06-01 00:00:00

abstract：:Salicylate is an important intermediate in the bacterial degradation of polycyclic aromatic hydrocarbons and salicylate hydroxylases play essential roles in linking the peripheral and ring-cleavage catabolic pathways. Unlike the well-characterized salicylate 1-hydroxylases, the rarely occurred salicylate 5-hydroxylase...

journal_title：Applied microbiology and biotechnology

authors： Fang T,Zhou NY

更新日期：2014-01-01 00:00:00

abstract：:Bacterial cells are known to adapt to challenging environmental conditions such as osmotic stress. However, most of the work done in this field describes the adaptation of growing populations where the new generations acquire traits that improve their ability to survive. In the present study, the responses of Rhodococ...

journal_title：Applied microbiology and biotechnology

authors： de Carvalho CC,Marques MP,Hachicho N,Heipieper HJ

更新日期：2014-06-01 00:00:00

abstract：:Catalytic antibodies are currently being investigated in order to understand their role under physio-pathological situations. To this end, the knowledge of structure-function relationships is of great interest. Recombinant scFv fragments are smaller and easier to genetically manipulate than whole antibodies, making th...

journal_title：Applied microbiology and biotechnology

authors： Ben Naya R,Matti K,Guellier A,Matagne A,Boquet D,Thomas D,Friboulet A,Avalle B,Padiolleau-Lefèvre S

更新日期：2013-09-01 00:00:00

abstract：:Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Di Gioia D,Marchetti L,Quattroni G

更新日期：1996-05-01 00:00:00