Abstract:
BACKGROUND:To promote understanding of immunoglobulin A nephropathy (IgAN) pathophysiology, we tried to elucidate glomerular protein profiles in IgAN, using microsieving that we established recently to isolate glomeruli from renal biopsy samples and proteomic approaches. METHODS:Glomeruli were isolated from renal biopsy samples of patients with IgAN (n = 5) and with minimal change nephrotic syndrome (MCNS; n = 5) using microsieving. Proteins extracted from the isolated glomeruli were separated by 2-dimensional differential gel electrophoresis (2D-DIGE). Proteins with different amounts between the two groups were identified by mass spectrometry. One of the identified proteins, α-actinin-4 (ACTN4), was further analyzed by Western blotting, RT-polymerase chain reaction (PCR), and immunohistochemistry. RESULTS:By 2D-DIGE, 72 out of the detected 1,170 protein spots showed significantly different intensity between the two groups (p < 0.05). Thirty-four out of the 72 protein spots showed more than 1.5-fold or less than 1/1.5-fold intensity, out of which 16 protein spots were successfully identified. No microbial protein was identified. ACTN4 molecules with a low molecular weight of approximately 77 kDa were found to increase in the IgAN group. Lack of an N-terminal part of ACTN4 was demonstrated by Western blotting. No defect of mRNA for ACTN4 was evidenced by RT-PCR. Predominant existence of ACTN4 in capillary walls of glomeruli of IgAN patients was demonstrated by immunohistochemistry in glomerular sections of patients with IgAN. CONCLUSION:Use of microsieving enabled us to biochemically analyze glomerular proteins in renal biopsy samples from patients with glomerular diseases. With this method, we demonstrated skewed glomerular protein profiles in IgAN.
journal_name
Am J Nephroljournal_title
American journal of nephrologyauthors
Kojima S,Koitabashi K,Iizuka N,Okamoto K,Arito M,Sato T,Kurokawa MS,Suematsu N,Shibagaki Y,Yasuda T,Kimura K,Kato Tdoi
10.1159/000357788subject
Has Abstractpub_date
2014-01-01 00:00:00pages
36-45issue
1eissn
0250-8095issn
1421-9670pii
000357788journal_volume
39pub_type
杂志文章abstract::In general, nonrheumatic atrial fibrillation is associated with a high risk of stroke. However, its impact on stroke in the setting of chronic hemodialysis treatment is insufficiently addressed in the literature. We assessed the incidence of stroke among 430 chronic hemodialysis patients and the impact of atrial fibri...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000046216
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Recent reports have highlighted that diabetic patients with kidney failure are at increased risk of technique failure and transfer to haemodialysis within 90 days of initiating peritoneal dialysis (PD). We wished to determine whether there were differences between diabetic and non-diabetic patients within th...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000477326
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:A poor nutritional state is associated with an increased risk of cardiovascular events in end-stage renal disease. It is unclear whether malnutrition itself can induce cardiovascular disease (CVD). We examined the impact of nutritional status on patients' arterial stiffness in peritoneal dialysis (PD) patien...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000206697
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND/AIM:While the familial nature of chronic kidney disease (CKD) has been recognized, it has primarily been defined from studies of first-degree relatives of selected sets of cases. The goal of this study is an evaluation of the familial clustering of end-stage renal disease (ESRD) and CKD mortality using a pop...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000092280
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND/AIMS:There is evidence that hereditary predisposition contributes to the development of diabetic nephropathy and hypertension. Polymorphisms in the genes for bradykinin receptors (B(1)R and B(2)R) were found to be associated with decreased risk of the development of end-stage renal disease. This study examin...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000068035
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Almost 20 million people in the US have chronic kidney disease (CKD). Cardiovascular disease and arterial wall abnormalities are common in this population. Because angiotensin II may have adverse effects on the arterial wall, we hypothesized that an angiotensin receptor blocker (ARB) would improve arterial c...
journal_title:American journal of nephrology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000087211
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND/AIMS:Cystic epithelia in polycystic kidney disease display features similar to malignant cells. Thiazolidinediones have been shown to have anti-neoplastic properties, therefore we tested the hypothesis that pioglitazone reduces cyst formation, improves renal function, and prolongs survival in a mouse model o...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000242432
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Older adults with advanced non-dialysis-dependent chronic kidney disease (NDD-CKD) face a high risk of hospitalization and related adverse events. METHODS:This prospective cohort study followed nephrology clinic patients ≥60 years old with NDD-CKD stages 4-5. After an eligible patient's office visit, study ...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000509046
更新日期:2020-01-01 00:00:00
abstract::We studied plasma, dialysate, and muscle carnitine levels in patients with stable chronic renal failure on hemodialysis, and intermittent peritoneal, or continuous ambulatory peritoneal dialysis (CAPD). In patients on hemodialysis, plasma carnitine levels fell from 46.2 +/- 4.5 mumol/l (mean +/- SEM) to 18.8 +/- 2.7 m...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000166711
更新日期:1983-07-01 00:00:00
abstract::The pathogenesis of calciphylaxis, a potentially life-threatening condition, is not well understood. Several factors such as end-stage renal disease (azotemia), hyperparathyroidism, hyperphosphatemia, hypercalcemia, a high calcium-phosphate product, and the use of steroids and cytotoxic drugs after kidney transplantat...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000169021
更新日期:1996-01-01 00:00:00
abstract:AIMS:This study investigated the protective effect of the angiotensin-converting enzyme inhibitor, enalapril, and the vitamin D analog, paricalcitol, alone or in combination, on cardiac oxidative stress in uremic rats. METHODS:Rats were made uremic by 5/6 nephrectomy and treated for 4 months as follows: (1) uremic + v...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000178251
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:There is continued and significant debate regarding the salient etiologies associated with graft loss and racial disparities in kidney transplant recipients. METHODS:This was a longitudinal cohort study of all adult kidney transplant recipients, comparing patients with early graft loss (<5 years) to those w...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000363393
更新日期:2014-01-01 00:00:00
abstract::Tubulointerstitial disease is an invariant finding in proteinuric renal disease regardless of the underlying disease or the compartment in which the disease originates. Such histologic changes are functionally significant in that scores of such injury rather than glomerular histologic injury correlate with decrements ...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000168652
更新日期:1993-01-01 00:00:00
abstract::We report on a Mycobacterium marinum infection in a diabetic woman 8 years after undergoing a combined pancreas-kidney transplantation. This is, to our knowledge, the first case report on an isolated skin infection with atypical mycobacteria after simultaneous pancreas-kidney transplantation. A genetic probe categoriz...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000065271
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:Apolipoprotein A1 (APOL1) gene variants occurring in people of West African descent contribute to the greater burden of kidney disease among African Americans. These variants are associated with increased risk of nondiabetic nephropathy, more rapid progression of chronic kidney disease, and shorter survival ...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000502675
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:Advanced glycation end products (AGEs) induce epithelial mesenchymal transition (EMT) in renal proximal tubular epithelial cells (PTECs) by promoting the two EMT regulators, transforming growth factor beta (TGF-β) and connective tissue growth factor (CTGF). However, the exact signaling mechanism remains...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000381464
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Serum protein profiling patterns can reflect the pathological state of a patient and therefore may be useful for clinical diagnostics. Here, we present results from a pilot study of proteomic expression patterns in hemodialysis patients designed to evaluate the range of serum proteomic alterations in this po...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000077409
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Uric acid has been reported to be a risk factor for the development of chronic kidney disease; however, no study has examined whether uric acid may confer a risk for acute kidney injury. METHODS:We investigated the relation between serum uric acid and the incidence of postoperative acute kidney injury in pa...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000238824
更新日期:2009-01-01 00:00:00
abstract::The relationship of the duration of anuria to the recovery in glomerular filtration rate (GFR) was studied in 71 children with diarrhea-associated hemolytic uremic syndrome. A significant relationship was found, and regression analysis revealed that y = 114.61 - 5.68 x, where y is predicted GFR (ml/min/1.73 m2) and x ...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168617
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Protein-energy wasting (PEW) is common and associated with poor outcome in hemodialysis patients. In hemodialysis patients, geriatric nutritional risk index (GNRI) and decoy receptor 3 (DcR3) have been shown as the nutritional and inflammatory markers, respectively. The present study aimed to assess the pred...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000366457
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND/AIM:Although clinical and immunological findings in steroid-sensitive nephrotic syndrome (SSNS) favor an immunopathogenesis, many issues remain unsolved. Comprehensive studies analyzing cellular and humoral immunity in SSNS are scarce, and few studies addressed the effect of steroids on immunological factors...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000085357
更新日期:2005-03-01 00:00:00
abstract::Administration of a single dose of 1,25-OH(2)D(3) can lower PTH levels for up to 4 days in chronic hemodialysis patients. Our purpose was to verify the effects of the same weekly dose of calcitriol per os given in one, two or three administrations, to patients on dialysis with secondary hyperparathyroidism. Thirty pat...
journal_title:American journal of nephrology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000046197
更新日期:2000-11-01 00:00:00
abstract:BACKGROUND:Prolonged use of corticosteroids continues to be the mainstay in the management of most proteinuric glomerulopathies, but is limited by extensive side effects. Alternative medications such as adrenocorticotropic hormone (ACTH) have been recently used to treat refractory glomerulopathies and have shown superi...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000506854
更新日期:2020-01-01 00:00:00
abstract::Therapeutic use of D-penicillamine has been associated with a range of adverse effects. We present a patient with scleroderma, treated with D-penicillamine for 5 years, who developed severe renal failure due to rapidly progressive glomerulonephritis with both crescents and subepithelial immune deposits on renal biopsy...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000169183
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Hyperoxaluria may result from increased endogenous production or overabsorption of dietary oxalate in the gastrointestinal tract leading to nephrolithiasis and, in some, to oxalate nephropathy and chronic kidney disease. ALLN-177 is an oral formulation of a recombinant, oxalate specific, microbial enzyme oxa...
journal_title:American journal of nephrology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000448766
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIMS:Current status of primary hyperoxaluria (PH) has not been surveyed in Japan. METHODS:Japanese patients with PH were reviewed in the published literature. RESULTS:Fifty-nine patients were diagnosed as PH from 1962 to 2003. The median ages both at diagnosis and at the onset of initial symptoms were 17 (...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000086361
更新日期:2005-05-01 00:00:00
abstract::A 22-year-old woman presented glomerulonephritis with Schönlein-Henoch-like syndrome and monoclonal abnormality. One month later, she developed a rapidly progressive glomerulonephritis with hypertension and persistent purpura. In the two renal biopsies performed during the first and the second attack, mesangial expans...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168276
更新日期:1991-01-01 00:00:00
abstract::This study investigated the possible beneficial effect of intraperitoneal proteolytic enzyme administration on the development of hypertension-induced renal injury in the rat model of 2-kidney 1-clip (2K1C) Goldblatt hypertension. Male Wistar rats (120-150 g) underwent either sham surgery (control, n = 5) or clipping ...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000013411
更新日期:1998-01-01 00:00:00
abstract::To evaluate the determinants of rhabdomyolysis in the diabetic state, we compared biochemical and clinical features of diabetic patients with (group 1, 41 patients) and without (group 2, 36 patients) rhabdomyolysis. There was no difference in values for serum potassium, bicarbonate, phosphate and calcium between the t...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168357
更新日期:1991-01-01 00:00:00
abstract::In hypoaldosteronemic patients with chronic renal insufficiency, administration of a mineralocorticoid steroid such as fludrocortisone can ameliorate hyperkalemia and metabolic acidosis, but this therapy is not always safe owing to the deleterious consequences of extracellular fluid volume expansion resulting from min...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000166827
更新日期:1984-01-01 00:00:00