Abstract:
:This study investigated the possible beneficial effect of intraperitoneal proteolytic enzyme administration on the development of hypertension-induced renal injury in the rat model of 2-kidney 1-clip (2K1C) Goldblatt hypertension. Male Wistar rats (120-150 g) underwent either sham surgery (control, n = 5) or clipping of the left renal artery. From day one 2K1C rats were randomized into 2 groups, placebo treatment (n = 7), and proteolytic enzyme treatment (n = 9). To the verum group a fixed mixture of trypsin (2.42 mg), bromelain (4.54 mg), and rutin (5.04 mg) dissolved in 2 ml of sterile 0.9% NaCl was administered intraperitoneally daily, while the placebo group received only vehicle. Rats were pair-fed. The duration of the study was 7 weeks. All 2K1C rats developed hypertension and the mean values of systolic blood pressure (SBP) did not differ significantly between the groups at any time recorded (SBP at sacrifice: controls 122.0 +/- 8.5 mm Hg; placebo 191.4 +/- 7. 6 mm Hg; enzyme 180.5 +/- 6.5 mm Hg). Enzyme treatment prevented the rise in proteinuria (controls 12.4 +/- 2.6 mg/24 h; placebo 19.7 +/- 3.9 mg/24 h; enzyme 12.2 +/- 1.3 mg/24 h; p < 0.05) and ameliorated the increase in serum urea concentrations. Histomorphologically, signs of malignant nephrosclerosis were not found in control rats, while they were present in 4/7 (57%) of placebo-treated rats, but only in 1/9 (11%) of the enzyme-treated group. The volume fraction of renocortical interstitium was increased in both 2K1C groups in comparison with controls; however, enzyme treatment decreased the accumulation of interstitial tissue significantly (-22%) compared to placebo treatment. Cellular infiltration with mononuclear cells was also lower in the protease-treated group. To summarize, in the rat model of 2K1C hypertension, systemic treatment with proteases ameliorates the severity of nephrosclerosis and tubulointerstitial fibrosis in the non-clipped kidney, as well as proteinuria, without affecting high blood pressure.
journal_name
Am J Nephroljournal_title
American journal of nephrologyauthors
Sebeková K,Dämmrich J,Fierlbeck W,Krivosiková Z,Paczek L,Heidland Adoi
10.1159/000013411subject
Has Abstractpub_date
1998-01-01 00:00:00pages
570-6issue
6eissn
0250-8095issn
1421-9670pii
13411journal_volume
18pub_type
杂志文章abstract::The development of new therapies for chronic diseases, such as diabetic kidney disease (DKD), will continue to be hampered by lack of sufficient biomarkers that will provide insights and will be responsive to treatment interventions. The recent application of metabolomic technologies, such as nuclear magnetic resonanc...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000447954
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cisplatin (CIS) induces nephrotoxicity partly through renal vasoconstriction and decreased glomerular filtration effects thought to involve adenosine acting on adenosine A(1) receptors (A1Rs). We studied the effect of the orally active, A1R antagonist tonapofylline (BG9928) on biochemical measures of re...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000248762
更新日期:2009-01-01 00:00:00
abstract::Accelerated atherosclerosis is both a cause and a consequence of chronic renal failure. Vascular smooth muscle cells (VSMCs) are an important component of atherosclerotic plaques, responsible for promoting plaque stability in advanced lesions. In contrast, VSMC apoptosis has been implicated in a number of deleterious ...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000097815
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Nephrology research is expanding, and harnessing the much-needed information and data for the practice of evidence-based medicine is becoming more challenging. In this study, we used the natural language processing and text mining approach to mitigate some of these challenges. METHODS:We analyzed 17,412 abs...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000504871
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Health-related quality of life (HRQOL) reflects a patient's perceived disease burden, treatment effectiveness, and health status. Given the time burden and physiologic effects of hemodialysis, patients who spend dialysis time (9-15 h/week) physically or intellectually engaged may have better HRQOL. We charac...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000492623
更新日期:2018-01-01 00:00:00
abstract::We describe an elderly women who died of renal failure secondary to spontaneous renal atheroembolic disease. The sole clinical clue to this diagnosis was a profound eosinophilia up to 19,100/mm3 and a relative eosinophil count of 80%. Renal atheroembolic disease should be a prominent consideration in any patient with ...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000168476
更新日期:1992-01-01 00:00:00
abstract::The objective was to evaluate the effect of high-flux hemodialysis on quality of life, intra- and interdialytic symptoms and neuropsychological function. The study was double-blind single cross-over with random allocation to order of treatment. The patients were stable adult hospital hemodialysis patients. Both the co...
journal_title:American journal of nephrology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000168491
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Tacrolimus (FK) is currently widely used in transplant immunosuppression and the treatment of autoimmune diseases. However, FK induces nephrotoxicity which is characterized by functional and structural renal injury. The ubiquitous protein annexin A1 (ANXA1) has potent anti-inflammatory effects and protects a...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000309756
更新日期:2010-01-01 00:00:00
abstract::During the period from April 4, 1988, to April 20, 1988, nine pyrogenic reactions and five gram-negative bacteremias occurred in 11 patients undergoing dialysis. All pyrogenic reactions and gram-negative bacteremias occurred among patients in whom a reprocessed dialyzer was used. The rate of pyrogenic reactions or bac...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168155
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cystic epithelia in polycystic kidney disease display features similar to malignant cells. Thiazolidinediones have been shown to have anti-neoplastic properties, therefore we tested the hypothesis that pioglitazone reduces cyst formation, improves renal function, and prolongs survival in a mouse model o...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000242432
更新日期:2009-01-01 00:00:00
abstract::A young woman presented with nephrotic syndrome due to membranous nephropathy and a localized form of giant lymph node hyperplasia (Castleman's disease) occurring as a large intra-abdominal mass. Five months after surgical removal of the mass, only mild proteinuria persisted. Twenty months later a second kidney biopsy...
journal_title:American journal of nephrology
pub_type: 杂志文章,评审
doi:10.1159/000168127
更新日期:1990-01-01 00:00:00
abstract::In order to evaluate the changes in causes and outcome of acute renal failure (ARF) during the years 1975-1989, 710 patients treated in our dialysis center were analyzed. We compared the etiology, the severity and catabolic state of ARF, the techniques of renal replacement therapy, which were employed and the ages and...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168466
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:While chronic dialysis treatment has been suggested to increase pulmonary pressure values, right ventricular dysfunction (RVD) is a major cause of death in patients with end-stage renal disease. We investigated the impact of different dialysis treatments on right ventricular function. METHODS:We examined 22...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000320755
更新日期:2010-01-01 00:00:00
abstract::Whole plasma from 6 normolipidemic chronic renal failure (CRF) patients undergoing hemodialysis treatment was passed through the anti-apolipoprotein (Apo) AI immunosorbent column connected to the anti-Apo B immunoaffinity column. Apo AI and B containing particles were analyzed for lipid and Apo contents. The results w...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000169011
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:There has been an increasing incidence of hemodialysis (HD) due to old age and comorbid condition such as diabetes. In general, socioeconomic status (SES) is known as one of the most important risk factors for patient mortality and morbidity. Whether low SES is associated with poorer outcome in HD patients i...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000512855
更新日期:2021-01-13 00:00:00
abstract::Maimonides (Moses ben Maimon) was born in 1135 in Cordoba, the son of a Jewish rabbi. After a seminomadic upbringing in Spain and North Africa during the rule of the Almohades, Maimonides settled in Fostate (Old Cairo), where he became renowned as a physician, eventually being appointed as court physician to Saladin a...
journal_title:American journal of nephrology
pub_type: 传,历史文章,杂志文章
doi:10.1159/000168739
更新日期:1994-01-01 00:00:00
abstract::A patient with renal failure due to myeloma kidney and coincident digitalis intoxication due to prescribed daily digoxin administration was treated with digoxin-specific antibody fragments and plasmapheresis. Rapid response to therapy was noted, removal of digoxin-antidigoxin antibody complexes was confirmed, and prev...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168179
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Evidence suggests that recombinant human erythropoietin (rHuEPO) protects neurons and cardiomyocytes from acute insults. We investigated the protective effect of rHuEPO on cyclosporine (CsA)-induced renal injury. METHODS:CsA (15 mg/kg/day) was given to rats for 1 or 4 weeks, and rHuEPO was concurrently admi...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000084275
更新日期:2005-01-01 00:00:00
abstract::Five patients with the clinical patterns of Bartter's syndrome underwent a series of clearance studies in order to characterize the underlying tubule defect. Free water generation during maximal water diuresis (CH2O), expressed as percentage of the distal delivery (CH2O + CCl), was lower in the patients (72.5 +/- 3.2%...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168451
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Chronic metabolic acidosis is a common metabolic disturbance and its clinical impact can be severe and extensive. The role and the change of the intrarenal renin-angiotensin system (RAS) during metabolic acidosis are uncertain, and whether acidosis can evoke inflammation remains unclear. METHODS:Male Spragu...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000328742
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND/AIMS:Although the effect of exercise on health is well established, nephrologists seldom consider physical activity in the treatment of chronic kidney disease (CKD) or CKD in the presence of diabetes mellitus (DM/CKD). The aim of the present study was to analyze the benefits of leisure-time physical activity...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000357133
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Three million people in the United States are estimated to have chronic kidney disease (CKD). Management of these CKD patients in the outpatient primary care clinic setting has not been well studied. HYPOTHESIS:Primary care management of CKD can be assessed and opportunities for improvement can be identifie...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000066296
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:2009 influenza A has spread globally. Respiratory complications and renal failure have been the leading causes for hospitalization and critical illness. We describe the risk factors and complications of acute kidney injury (AKI) in patients with influenza A. METHODS:Observational study of adult patients tes...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000328386
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Vascular calcification is seen in most patients on dialysis and is strongly associated with cardiovascular mortality. Vascular calcification is promoted by phosphate, which generally reaches higher levels in hemodialysis than in peritoneal dialysis. However, whether vascular calcification develops less in pe...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000494665
更新日期:2018-01-01 00:00:00
abstract::This study describes 10 cases of IgM nephropathy in whom the main morphological findings consisted of diffuse mesangial deposition of IgM and varying degrees of mesangial cell proliferation. In addition, focal segmental sclerosis was present in 1 patient and global sclerosis in another. An ill-defined electron-dense d...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000167485
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND/AIMS:The elderly are the fastest growing subpopulation with end-stage renal disease. The goal of our study was to define characteristics of elderly patients who were considered ineligible for transplantation compared to those who were listed. METHODS:984 patients were referred for evaluation during a 2-year...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000354483
更新日期:2013-01-01 00:00:00
abstract::Skin ulceration is a well-characterized thrombotic complication of the heparin-induced thrombocytopenia (HIT) syndrome. We present the case of a 73-year-old diabetic woman nearing end-stage renal failure who developed extensive upper thigh, abdominal and buttock ulceration following initiation of subcutaneous heparin ...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000046263
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND:Kidney failure is believed to have a negative impact on cognitive function, and cognitive impairment is common among maintenance hemodialysis (HD) patients. Previous studies have shown a beneficial effect of kidney transplantation in certain cognitive tests but not across all cognitive domains assessed. But,...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000330849
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Transglutaminase 2 (TGM2) is a calcium-dependent enzyme that can cross-link nearly all extracellular matrix (ECM) proteins and can facilitate cell-ECM interaction through integrins. Given the importance of the ECM in vascular calcification we tested the hypothesis that increased TGM2 activity may accelerate ...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000347031
更新日期:2013-01-01 00:00:00
abstract::Radioisotopic methods for the determination of the glomerular filtration rate (GFR) are highly accurate but require the collection of multiple blood and urine samples and are costly to perform due to personnel, material, and analysis costs. Nonradioactive methods of GFR determination have the potential of minimizing p...
journal_title:American journal of nephrology
pub_type: 杂志文章
doi:10.1159/000168985
更新日期:1996-01-01 00:00:00