Abstract:
:The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase fromEuglena gracilis decays steadily when exposed to agents that induce oxidative modification of cysteine residues (Cu(2+), benzofuroxan, disulfides, arsenite, oxidized ascorbate). Inactivation takes place with a concomitant loss of cysteine sulfhydryl groups and dimerization of large subunits of the enzyme. 40% activity loss induced by the vicinal thiol-reagent arsenite is caused by modification of a few neighbor residues while the almost complete inactivation achieved with disulfides is due to extensive oxidation leading to formation of mixed disulfides with critical cysteines of the protein. In most cases oxidative inactivation is also accompanied by an increased sensitivity to proteolysis by trypsin, chymotrypsin or proteinase K. Both enzymatic activity and resistance to proteolysis can be restored through treatment with several thiols (cysteamine, cysteine, dithiothreitol and, more slowly, reduced glutathione). Redox effectors which are thought to regulate the chloroplast activity (NADPH, ferredoxin and thioredoxin) do not reactivate the oxidized enzyme. When ribulose-1,5-bisphoshate carboxylase/oxygenase is incubated with cystamine/cysteamine mixtures having different disulfide/thiol ratio (r), inactivation takes place around r=1.5 while proteolytic sensitization occurs under more oxidative conditions (r=4). It is suggested that oxidative modification may happen in vivo under exceptional circumstances, such as senescence, bleaching or different kinds of stress, leading to enzyme inactivation and triggering the selective degradation of the carboxylase that has been repeatedly observed during these processes.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
García-Ferris C,Moreno Jdoi
10.1007/BF02185411subject
Has Abstractpub_date
1993-01-01 00:00:00pages
55-66issue
1eissn
0166-8595issn
1573-5079journal_volume
35pub_type
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journal_title:Photosynthesis research
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更新日期:2006-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2007-07-01 00:00:00
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更新日期:1993-07-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016274
更新日期:1993-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-11-01 00:00:00
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更新日期:1994-07-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9828-z
更新日期:2013-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00