Comparison of photosystem II complexes isolated from tobacco and two chlorophyll deficient tobacco mutants.

Abstract:

:A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids with occasional membrane doublings, has been carried out. The corresponding photosystem II complexes had an O2 evolving activity ranging from 290 (for the wild type) to 1100 μmol O2 x mg chlorophyll(-1) x h(-1) (for the mutant Su/su var. Aurea). The reduced photosynthetic unit size was also obvious in the mangenese and cytochromeb559 content. The photosystem II complex from the wild type contained 4 Mn and 1 cytochromeb559 per 200 to 280 chlorophylls, while the corresponding value for the mutant Su/su var. Aurea was 4 Mn and 1 cytochromeb559 per 35 to 60 chlorophylls. We have also examined the polypeptide composition and show that the photosystem II complex from the wild type consisted of polypeptides of 48, 42, 33, 32, 30, 28, 23, 21, 18, 16 and 10 kDa, while the mutant complex mainly contained the polypeptides of 48, 42, 33, 32, 30, 28 and 10 kDa. In the mutant photosystem II complex the light-harvesting chlorophyll protein (peptide of 28 kDa) was reduced by a factor of 5 to 6 as compared to the wild type. With respect to the peptide composition and the photosynthetic unit size, the Triton-solubilized photosystem II complex from the mutant Su/su var. Aurea was very similar to O2 evolving photosystem II reaction center core complexes.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Specht S,Pistorius EK,Schmid GH

doi

10.1007/BF00032264

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

47-56

issue

1

eissn

0166-8595

issn

1573-5079

journal_volume

13

pub_type

杂志文章
  • Photosystem II cycle activity and alternative electron transport in the diatom Phaeodactylum tricornutum under dynamic light conditions and nitrogen limitation.

    abstract::Alternative electron sinks are an important regulatory mechanism to dissipate excessively absorbed light energy particularly under fast changing dynamic light conditions. In diatoms, the cyclic electron transport (CET) around Photosystem II (PS II) is an alternative electron transport pathway (AET) that contributes to...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-015-0209-7

    authors: Wagner H,Jakob T,Lavaud J,Wilhelm C

    更新日期:2016-05-01 00:00:00

  • Long-distance translocation of photosynthates: a primer.

    abstract::The storage of light energy in chemical form through photosynthesis is the key process underlying life as we know it. To utilize photosynthates efficiently as structural materials or as fuel to drive endergonic processes, they have to be transported from where they are produced to where they are needed. In this primer...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-013-9867-5

    authors: Knoblauch M,Peters WS

    更新日期:2013-11-01 00:00:00

  • Photosynthetic responses of cassava cultivars (Manihot esculenta Crantz) from different habitats to temperature.

    abstract::Maximum photosynthetic CO2 exchange rates (Pn) of single attached leaves were determined for several cassava cultivars selected from different habitats and grown in pots outdoors at CIAT, Colombia, S.A. Pn rates were in a narrow range of 22 to 26 μmol CO2 m(-2)s(-1) for all cultivars tested when measured at high photo...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00030025

    authors: El-Sharkawy MA,Cock JH,Held AA

    更新日期:1984-09-01 00:00:00

  • Carbohydrate regulation of leaf development: Prolongation of leaf senescence in Rubisco antisense mutants of tobacco.

    abstract::Previous investigations have shown that increased source strength as a result of elevated CO(2) can alter the timing of the phases of change that occur in photosynthetic rates during dicot leaf ontogeny [Miller et al. (1997) Plant Physiol 115: 1195-1200]. To evaluate the converse situation of decreased source strength...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/A:1006367719639

    authors: Miller A,Schlagnhaufer C,Spalding M,Rodermel S

    更新日期:2000-01-01 00:00:00

  • The Soret absorption properties of carotenoids and chlorophylls in antenna complexes of higher plants.

    abstract::The absorption spectra of two light harvesting complexes from higher plants, CP29 and LHC II, have been analysed in the Soret region in order to obtain a description in terms of the absorption spectra of the individual pigments. This information is of great practical use when applying spectroscopic techniques to the s...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/A:1006455230379

    authors: Croce R,Cinque G,Holzwarth AR,Bassi R

    更新日期:2000-01-01 00:00:00

  • Interactions among leaf photosynthetic rates, flowering and pod set in soybeans.

    abstract::The seasonal maximum in photosynthetic CO2 exchange rate (CER) and the cessation of leaf expansion in soybeans (Glycine max (L.) Merr) accompany fruiting under normal agricultural conditions. To investigate whether these phenomena were obligatively tied together, we caused early flowering of long-season varieties by i...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00034108

    authors: Enos WT,Alfich RA,Hesketh JD,Woolley JT

    更新日期:1982-12-01 00:00:00

  • Chemical oxidation of the FMO antenna protein from Chlorobaculum tepidum.

    abstract::Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-013-9878-2

    authors: Bina D,Blankenship RE

    更新日期:2013-09-01 00:00:00

  • High-throughput pyrosequencing of the chloroplast genome of a highly neutral-lipid-producing marine pennate diatom, Fistulifera sp. strain JPCC DA0580.

    abstract::The chloroplast genome of the highly neutral-lipid-producing marine pennate diatom Fistulifera sp. strain JPCC DA0580 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918 bp ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-011-9622-8

    authors: Tanaka T,Fukuda Y,Yoshino T,Maeda Y,Muto M,Matsumoto M,Mayama S,Matsunaga T

    更新日期:2011-09-01 00:00:00

  • The inhibitory effects of acidification and augmented oxygen pressure on water oxidation.

    abstract::Cyanobacteria, algae, and plants produce dioxygen from water. Driven and clocked by light quanta, the catalytic Mn(4)Ca Tyrosine centre accumulates four oxidizing equivalents before it abstracts four electrons from water and liberates dioxygen and protons. Intermediates of this reaction cascade are short-lived (<100 m...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-008-9321-2

    authors: Clausen J,Junge W

    更新日期:2008-10-01 00:00:00

  • Chlorosome proteins studied by MALDI-TOF-MS: topology of CsmA in Chlorobium tepidum.

    abstract::Chlorosomes, the light-harvesting apparatus of green bacteria, are a unique antenna system, in which pigments are organized in aggregates rather than associated with proteins. Isolated chlorosomes from the green sulphur bacterium Chlorobium tepidum contain 10 surface-exposed proteins. Treatment of chlorosomes from Chl...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-005-3757-4

    authors: Milks KJ,Danielsen M,Persson S,Jensen ON,Cox RP,Miller M

    更新日期:2005-11-01 00:00:00

  • Examination of abiotic cofactor assembly in photosynthetic biomimetics: site-specific stereoselectivity in the conjugation of a ruthenium(II) tris(bipyridine) photosensitizer to a multi-heme protein.

    abstract::To understand design principles for assembling photosynthetic biohybrids that incorporate precisely-controlled sites for electron injection into redox enzyme cofactor arrays, we investigated the influence of chirality in assembly of the photosensitizer ruthenium(II)bis(2,2'-bipyridine)(4-bromomethyl-4'-methyl-2,2'-bip...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-019-00697-8

    authors: Ponomarenko NS,Kokhan O,Pokkuluri PR,Mulfort KL,Tiede DM

    更新日期:2020-02-01 00:00:00

  • Light effects on leaf development and photosynthetic capacity of Hydrocotyle bonariensis Lam.

    abstract::Rates of net CO2 uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m(-2) day(-1) PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m(-2) day(-1)). Maximum net...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00028750

    authors: Longstreth DJ,Hartsock TL,Nobel PS

    更新日期:1981-06-01 00:00:00

  • Nigericin insensitive post-illumination reduction in fluorescence yield in Dunaliella tertiolecta (chlorophyte).

    abstract::Cells of the green alga Dunaliella tertiolecta grown in a light/dark cycle were exposed to high light for about 15 min. In light, energy-dependent quenching reduced fluorescence emission and decreased PS II efficiency. Within 3 minutes after darkening fluorescence quenching largely relaxed. However, PS II fluorescence...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00033120

    authors: Casper-Lindley C,Björkman O

    更新日期:1996-12-01 00:00:00

  • The use of photothermal radiometry in assessing leaf photosynthesis: I. General properties and correlation of energy storage to P700 redox state.

    abstract::Energy storage measurements by modulated photothermal radiometry (PTR) were carried out on intact leaves to assess the value of the PTR method for photosynthesis research. In particular, correlations to the redox state of P700 under various conditions were examined. PTR monitors modulated light conversion to heat by s...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00035379

    authors: Malkin S,Schreiber U,Jansen M,Canaani O,Shalgi E,Cahen D

    更新日期:1991-08-01 00:00:00

  • Monitoring Migration and Measuring Biomass in Benthic Biofilms: The Effects of Dark/far-red Adaptation and Vertical Migration on Fluorescence Measurements.

    abstract::Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/B:PRES.0000028397.86495.b5

    authors: Consalvey M,Jesus B,Perkins RG,Brotas V,Underwood GJ,Paterson DM

    更新日期:2004-01-01 00:00:00

  • Structural Basis of Redox Signaling in Photosynthesis: Structure and Function of Ferredoxin:thioredoxin Reductase and Target Enzymes.

    abstract::The role of the ferredoxin:thioredoxin system in the reversible light activation of chloroplast enzymes by thiol-disulfide interchange with thioredoxins is now well established. Recent fruitful collaboration between biochemists and structural biologists, reflected by the shared authorship of the paper, allowed to solv...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/B:PRES.0000017194.34167.6d

    authors: Dai S,Johansson K,Miginiac-Maslow M,Schürmann P,Eklund H

    更新日期:2004-01-01 00:00:00

  • Temperature-induced alterations of in vivo chlorophyll a fluorescence induction in cucumber as affected by DCMU.

    abstract::Induction of chlorophyll a fluorescence and photosynthesis as affected by temperature were measured in cucumber leaf discs. Abrupt changes of the maximal variable fluorescence, Fv(p), and photosynthesis were observed around 9° and 21°C when the temperature was decreased from 30° to 0°C. The temperature-dependent maxim...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00035259

    authors: Janssen LH,Van Hasselt PR

    更新日期:1988-02-01 00:00:00

  • The decline in photosynthetic rate upon transfer from high to low light is linked to the slow kinetics of chloroplast ATP synthase in Bletilla striata.

    abstract::Upon a sudden transition from high to low light, the rate of CO2 assimilation (AN) in some plants first decreases to a low level before gradually becoming stable. However, the underlying mechanisms remain controversial. The activity of chloroplast ATP synthase (gH+) is usually depressed under high light when compared ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-020-00725-y

    authors: Yang YJ,Zhang SB,Wang JH,Huang W

    更新日期:2020-04-01 00:00:00

  • Isolation and characterization of outer and inner envelope membranes of cyanelles from a glaucocystophyte, Cyanophora paradoxa.

    abstract::Envelope membranes were isolated by sucrose density gradient floatation centrifugation from the homogenate of cyanelles prepared from Cyanophora paradoxa. Two yellow bands were separated after 40 h of centrifugation. The buoyant density of one of the two fractions (fraction Y2) coincided with that of inner envelope me...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-007-9156-2

    authors: Koike H,Ikeda Y,Yusa F,Kashino Y,Satoh K

    更新日期:2007-07-01 00:00:00

  • Energy coupling, membrane lipids and structure of thylakoids of Lupin plants submitted to water stress.

    abstract::Bioenergetic properties of thylakoids from plants submitted to a water stress stress (watering stopped for 6-15 days) have been measured in two lupin genotypes characterized as resistant or susceptible to drought. This energy coupling was assessed by flow-force relationships relating the phosphorylation rate to the ma...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00035944

    authors: Meyer S,Phung Nhu Hung S,Trémolières A,de Kouchkovsky Y

    更新日期:1992-05-01 00:00:00

  • An LED-based fluorometer for chlorophyll quantification in the laboratory and in the field.

    abstract::The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the s...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-012-9777-y

    authors: Lamb JJ,Eaton-Rye JJ,Hohmann-Marriott MF

    更新日期:2012-10-01 00:00:00

  • From chloroplasts to chaperones: how one thing led to another.

    abstract::Two lessons I have learned during my research career are the importance of following up unexpected observations and realizing that the most obvious interpretation of such observations can be rational but wrong. When you carry out an experiment there is usually an expectation that the result will fall within a range of...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/B:PRES.0000030439.62331.d0

    authors: John Ellis R

    更新日期:2004-01-01 00:00:00

  • Tissue specific protochlorophyll(ide) forms in dark-forced shoots of grapevine (Vitis viniferaL.).

    abstract::Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-004-1061-3

    authors: Böddi B,Bòka K,Sundqvist C

    更新日期:2004-01-01 00:00:00

  • In one era and out the other.

    abstract::A guided tour through much of photosynthesis research as I saw it, 1936-2001, is presented here. For earlier perspectives, see Myers 1974 (Plant Physiol 54: 420-426) and 1996 (Photosynth Res 50: 195-208). ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/A:1020475502933

    authors: Myers J

    更新日期:2002-01-01 00:00:00

  • Ozone tolerance and antioxidant enzyme activity in soybean cultivars.

    abstract::The current study confirmed earlier conclusions regarding differential ozone (O(3)) tolerances of two soybean cultivars, Essex and Forrest, and evaluated antioxidant enzyme activities of these two varieties based on their performance under environmentally relevant, elevated O(3) conditions. The experiment was conducte...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/A:1026500911237

    authors: Chernikova T,Robinson JM,Lee EH,Mulchi CL

    更新日期:2000-01-01 00:00:00

  • The purple photosynthetic bacterium Rhodopseudomonas acidophila contains multiple puc peripheral antenna complex (LH2) genes: Cloning and initial characterisation of four β/α pairs.

    abstract::The genome of the purple non-sulphur photosynthetic bacterium Rhodopseudomonas acidophila has been found to contain multiple copies of puc light-harvesting (LH2) peripheral antenna complex genes. Three wild-type isolates each exhibiting dissimilar peripheral antenna complex phenotypes in response to growth at reduced ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00034783

    authors: Gardiner AT,Mackenzie RC,Barrett SJ,Kaiser K,Cogdell RJ

    更新日期:1996-09-01 00:00:00

  • The response to ribulose bisphosphate(4-) (RuBP (4-)) and RuBP-Mg (2-) in catalysis by structurally divergent RuBP carboxylase/oxygenases.

    abstract::Free ribulose hisphosphate (RuBP(4-)) rather than its magnesium complex (RuBP-Mg(2-)) was the apparent substrate for spinach ribulose bisphosphate carboxylase/oxygenase. The apparent Km for total RuBP (pH 8.0 at 30° C) increased with increasing Mg(2+) concentrations from 11.6 μM at 13.33 mM Mg(2+) to 32.6 μM at 40.33 ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00041806

    authors: Roach DJ,McFadden BA

    更新日期:1983-01-01 00:00:00

  • Light-harvesting features revealed by the structure of plant photosystem I.

    abstract::Oxygenic photosynthesis is driven by two multi-subunit membrane protein complexes, Photosystem I and Photosystem II. In plants and green algae, both complexes are composed of two moieties: a reaction center (RC), where light-induced charge translocation occurs, and a peripheral antenna that absorbs light and funnels i...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/B:PRES.0000036881.23512.42

    authors: Ben-Shem A,Frolow F,Nelson N

    更新日期:2004-01-01 00:00:00

  • Heliobacterial photosynthesis.

    abstract::Heliobacteria contain Type I reaction centers (RCs) and a homodimeric core, but unlike green sulfur bacteria, they do not contain an extended antenna system. Given their simplicity, the heliobacterial RC (HbRC) should be ideal for the study of a prototypical homodimeric RC. However, there exist enormous gaps in our kn...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-007-9162-4

    authors: Heinnickel M,Golbeck JH

    更新日期:2007-04-01 00:00:00

  • Exciton interactions in synthetic porphyrin dimers.

    abstract::The Soret absorption spectra of six synthetic rigid porphyrin dimers whose crystal structures have been determined are simulated using simple exciton theory. The objective is to test the validity of the point dipole and associated approximations; the electronic interaction parameters are thus calculated using data obt...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00048299

    authors: Won Y,Friesner RA,Johnson MR,Sessler JL

    更新日期:1989-12-01 00:00:00