Abstract:
:Chlorosomes, the light-harvesting apparatus of green bacteria, are a unique antenna system, in which pigments are organized in aggregates rather than associated with proteins. Isolated chlorosomes from the green sulphur bacterium Chlorobium tepidum contain 10 surface-exposed proteins. Treatment of chlorosomes from Chlorobium tepidum with protease caused changes in the spectral properties of bacteriochlorophyll c and digestion of chlorosome proteins. Using SDS-PAGE analysis, immunoblotting and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) we have investigated the topology of the 59-residue CsmA protein. Our results show that at the N-terminus, the only amino acid available for protease degradation is the methionine. At the C-terminus, amino acids can be removed by protease treatment to produce a residual protein containing at least the sequence between residues 2 and 38. These results indicate that the N-terminal portion of the CsmA protein, which is predicted to be mainly hydrophobic, is buried in the chlorosome envelope.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Milks KJ,Danielsen M,Persson S,Jensen ON,Cox RP,Miller Mdoi
10.1007/s11120-005-3757-4subject
Has Abstractpub_date
2005-11-01 00:00:00pages
113-21issue
1-2eissn
0166-8595issn
1573-5079journal_volume
86pub_type
杂志文章abstract::Water vapor was added differentially to the gas streams entering the cells of three makes of differential infrared carbon dioxide analysers. Analyser deflections were compared with those expected from dilution of the carbon dioxide by the additional gas. Tests were made at 0, 365, and 730 cm(3) m(-3) concentrations of...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054104
更新日期:1985-12-01 00:00:00
abstract::A chlorosome is an antenna complex located on the cytoplasmic side of the inner membrane in green photosynthetic bacteria that contains tens of thousands of self-assembled bacteriochlorophylls (BChls). Green bacteria are known to incorporate various esterifying alcohols at the C-17 propionate position of BChls in the ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0017-5
更新日期:2014-10-01 00:00:00
abstract::A study of the absorption and fluorescence characteristics of the D1/D2/cytb-559 reaction centre complex of Photosystem II has been carried out by gaussian decomposition of absorption spectra both at room temperature and 72 K and of the room temperature fluorescence spectrum. A five component fit was found in which th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02183048
更新日期:1994-09-01 00:00:00
abstract::Chlorosomes of green photosynthetic bacterium Chlorobium tepidum contain aggregates of bacteriochlorophyll c (BChl c) with carotenoids and isoprenoid quinones. BChl aggregates with very similar optical properties can be prepared also in vitro either in non-polar solvents or in aqueous buffers with addition of lipids a...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9259-9
更新日期:2008-02-01 00:00:00
abstract::The possible origins of the different fluorescence decay components in green plants are discussed in terms of a random walk and Butler's bipartite model. The interaction of the excitations with the photosystem II reaction centers and, specifically, the regeneration of theses excitations by charge recombination within ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00118288
更新日期:1986-01-01 00:00:00
abstract::The relationship between the redox state of P700, the primary donor of PS I, monitored using absorbance changes at 830 nm and photochemical energy storage in PS I reaction centers assayed with the photoacoustic method (PA) was studied in isolated PS I submembrane particles aspirated onto nitrocellulose filters. Severa...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1021237026500
更新日期:2002-01-01 00:00:00
abstract::Biological water oxidation, performed by a single enzyme, photosystem II, is a central research topic not only in understanding the photosynthetic apparatus but also for the development of water splitting catalysts for technological applications. Great progress has been made in this endeavor following the report of a ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-019-00648-3
更新日期:2019-10-01 00:00:00
abstract::Photoinhibition of the light-induced Photosystem I (PS I) electron transfer activity from the reduced dichlorophenol indophenol to methyl viologen was studied. PS I preparations with Chl/P700 ratios of about 180 (PS I-180), 100 (PS I-100) and 40 (PS I(HA)-40) were isolated from spinach thylakoid membranes by the treat...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016175
更新日期:1996-02-01 00:00:00
abstract::The effect of desiccation and rehydration on the function of Photosystem II has been studied in the desiccation tolerant lichen Cladonia convoluta by thermoluminescence. We have shown that in functional fully hydrated thalli thermoluminescence signals can be observed from the recombination of the S2(3)QB (-) (B band),...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041010
更新日期:1996-05-01 00:00:00
abstract::Polyamines (PAs) are small metabolites that are produced and oxidized in chloroplasts with an obscure mode of action. Recently, we showed that qE is stimulated by PAs in higher plants (Nicotiana tabacum) and in genetically modified plants with elevated thylakoid-associated PAs (Ioannidis and Kotzabasis Biochim Biophys...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9617-x
更新日期:2011-02-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii acclimates to low-CO2 (LC) conditions by actively transporting inorganic carbon (Ci) into the cell, resulting in an increase in photosynthetic efficiency. This mechanism is called the carbon-concentrating mechanism (CCM), and soluble protein LCIB is essential for th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9963-6
更新日期:2014-09-01 00:00:00
abstract::The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosy...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054145
更新日期:1983-12-01 00:00:00
abstract::Oxygenic photosynthesis requires chlorophyll (Chl) for the absorption of light energy, and charge separation in the reaction center of photosystem I and II, to feed electrons into the photosynthetic electron transfer chain. Chl is bound to different Chl-binding proteins assembled in the core complexes of the two photo...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-015-0154-5
更新日期:2015-12-01 00:00:00
abstract::Two lessons I have learned during my research career are the importance of following up unexpected observations and realizing that the most obvious interpretation of such observations can be rational but wrong. When you carry out an experiment there is usually an expectation that the result will fall within a range of...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000030439.62331.d0
更新日期:2004-01-01 00:00:00
abstract::Dry weight and Relative Growth Rate of Lemna gibba were significantly increased by CO2 enrichment up to 6000 μl CO2 l(-1). This high CO2 optimum for growth is probably due to the presence of nonfunctional stomata. The response to high CO2 was less or absent following four days growth in 2% O2. The Leaf Area Ratio decr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029048
更新日期:1985-03-01 00:00:00
abstract::Minimal photosynthetic catalytic F1(αβ) core complexes, containing equimolar ratios of the α and β subunits, were isolated from membrane-bound spinach chloroplast CF1 and Rhodospirillum rubrum chromatophore RrF1. A CF1-α3β3 hexamer and RrF1-α1β1 dimer, which were purified from the respective F1(αβ) complexes, exhibit ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020418
更新日期:1995-11-01 00:00:00
abstract::A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-depe...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054124
更新日期:1991-06-01 00:00:00
abstract::NADP:ferredoxin oxidoreductase (EC. 1.6.7.1.) isolated from a thermophilic blue-green alga, Synechococcus sp., was stable at temperatures up to 65°C. The diaphorase and cytochrome c reductase activities of the enzyme were low at 25°C but increased with elevated temperature to reach a maximum at about 60°C. The pH-prof...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020595
更新日期:1980-09-01 00:00:00
abstract::The study of the light-harvesting complex II (LHC-II) phosphatase activity has been difficult due to the membrane association of its substrate. Thylakoid membranes labeled with [γ-(32)P]ATP were incubated with chymotrypsin, releasing phosphopeptides which served as labeled substrates for LHC-II phosphatase. Utilizing ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018301
更新日期:1995-05-01 00:00:00
abstract::Species in the Laxa and Grandia groups of the genus Panicum are adapted to low, wet areas of tropical and subtropical America. Panicum milioides is a species with C3 photosynthesis and low apparent photorespiration and has been classified as a C3/C4 intermediate. Other species in the Laxa group are C3 with normal phot...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00024189
更新日期:1986-01-01 00:00:00
abstract::Data on cadmium accumulation in chloroplasts of terrestrial plants are scarce and contradictory. We introduced CdSO4 in hydroponic media to the final concentrations 80 and 250 μM and studied the accumulation of Cd in chloroplasts of Hordeum vulgare and Zea mays. Barley accumulated more Cd in the chloroplasts as compar...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0047-z
更新日期:2015-08-01 00:00:00
abstract::In appreciation of his contribution to the Photosystsem II research and commemoration of the book Photosystem II: The Light-Driven Water-Plastoquinone Oxido-Reductase, co-edited with Kimiyuki Satoh, I present here some of my recollections of Thomas John Wydrzynski and by several others with whom he has associated over...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-008-9341-y
更新日期:2008-10-01 00:00:00
abstract::Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000028397.86495.b5
更新日期:2004-01-01 00:00:00
abstract::We present the wavelength dependence of homogeneous holewidths of persistent spectral holes burnt in O2-evolving Photosystem II core complexes isolated from spinach, in the temperature range 2.5-8 K. The data supports the assignment that those chlorophylls which undergo persistent spectral hole-burning are specific CP...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7927-6
更新日期:2005-06-01 00:00:00
abstract::A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033446
更新日期:1992-11-01 00:00:00
abstract::The functional peculiarities and responses of the photosynthetic system in the flowering homoiochlorophyllous desiccation-tolerant (HDT) Haberlea rhodopensis and the non-desiccation-tolerant spinach were compared during desiccation and rehydration. Increasing rate of water loss clearly modifies the kinetic parameters ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-2440-0
更新日期:2005-08-01 00:00:00
abstract::Productivity of most macroalgae is not currently considered limited by dissolved inorganic carbon (DIC), as the majority of species have CO2-concentrating mechanisms (CCM) allowing the active uptake of DIC. The alternative, diffusive uptake of CO2 (non-CCM), is considered rare (0-9% of all macroalgal cover in a given ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0114-0
更新日期:2015-05-01 00:00:00
abstract::The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9244-3
更新日期:2008-02-01 00:00:00
abstract::Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00036216
更新日期:1991-09-01 00:00:00
abstract::The afterglow (AG) band of thermoluminescence (TL) has been investigated in leaves of Arabidopsis thaliana. Excitation of dark-adapted leaves with two saturating single turn-over flashes induced the appearance of a complex TL glow curve that could be well simulated by three components: the two components, B1 and B2, o...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7311-6
更新日期:2005-06-01 00:00:00